Separation and identification of individual anthocyanins from the petals of some rose cultivars

Authors

  • Ionela C. PASERE University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Department of Biochemistry, Faculty of Animal Science and Biotechnology, 3-5 Manastur St., 400372 Cluj-Napoca (RO)
  • Gabriela M. ROMAN University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Horticultural Research Station, 5 Horticultorilor St., Cluj-Napoca (RO)
  • Claudiu I. BUNEA University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Department of Horticulture and Landscape, 3-5 Manastur St., 400372 Cluj-Napoca (RO)
  • Andrea BUNEA University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Department of Biochemistry, Faculty of Animal Science and Biotechnology, 3-5 Manastur St., 400372 Cluj-Napoca (RO)
  • Alexandru NICOLESCU University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Department of Horticulture and Landscape, 3-5 Manastur St., 400372 Cluj-Napoca (RO)
  • Adela PINTEA University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Department of Biochemistry, Faculty of Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-Napoca (RO)

DOI:

https://doi.org/10.15835/nbha52313772

Keywords:

anthocyanins, antioxidants, HPLC, total phenolic content, total flavonoid content, rose cultivars

Abstract

Rose cultivars represent a source with potential for therapeutical applications due to the high content in bioactive phytochemicals, including phenolic derivatives and lipophilic compounds, such as carotenoids. Anthocyanins, a phenolic subclass, represent the major compounds responsible for the colors of roses. In this study, we have aimed to extract, separate, and identify the anthocyanin compounds found in the petals of 25 different rose cultivars. High performance liquid chromatography followed by electrospray ionization mass spectrometry (HPLC-ESI-MS) was used to separate, identify and quantify the individual anthocyanins. The results showed the presence of 9 anthocyanin compounds, whereas the total anthocyanin content of rose cultivars ranged from 20.267-1206.841 mg/100 g FW, from which cyanidin-caffeoyl-glucoside was the most quantitatively represented anthocianin, followed by cyanidin-glucoside and pelargonidin-glucoside. In addition to this, the antioxidant activity was applied using the established DPPH in vitro method (1403 ± 301-304 ± 37 µmol TE/g FW), as well as the total phenolic content (2600±452-208±23 mg GAE/100 g FW) and the total flavonoid content (160±23-705±49 mg QE/100 g FW). Among the evaluated cultivars, the highest content in anthocyanin was determined for the ‘Amalia’ cultivar. Between the 25 rose cultivars analyzed, the varieties ‘Amalia’, ‘Foc de tabără’, ‘Fruhrot’, and ‘First Red’ followed as being the richest in anthocyanins content and the lowest values were recorded for the varieties ‘Jimini Cricket’, ‘Cri-Cri’, ‘Fortuna’, and ‘Zburlici’.  The same trend was observed for the total phenolic and total flavonoid content but also the antioxidant activity. The importance of analyzing anthocyanin content in different rose varieties came from the use of anthocyanins as natural dyes, having potential pharmaceutical ingredients that give various beneficial health effects.

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Published

2024-09-29

How to Cite

PASERE, I. C., ROMAN, G. M., BUNEA, C. I., BUNEA, A., NICOLESCU, A., & PINTEA, A. (2024). Separation and identification of individual anthocyanins from the petals of some rose cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(3), 13772. https://doi.org/10.15835/nbha52313772

Issue

Vol. 52 No. 3 (2024)

Section

Research Articles
CITATION
DOI: 10.15835/nbha52313772

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