Protective role of biosynthesized silver nanoparticles synthesized using sesame oil as biocontrol approach against Erwinia amylovora causing fire blight in pears (Pyrus communis L.)

Authors

  • Mohamed S. ATTIA Al-Azhar University, Faculty of Science, Botany & Microbiology Department, Nasr City, Cairo (EG)
  • Mohamed A. ABDALWAHAB Horticulture Research Institute, Agricultural Research Center, Giza (EG)
  • Adel A. AWAD Agricultural Microbiology Research Department, Soils, Water &Environment Research Institute (SWERI), Agricultural Research Center (ARC), Giza (EG)
  • Eslam K. KANDIL Al-Azhar University, Faculty of Science, Botany & Microbiology Department, Nasr City, Cairo (EG)
  • Noha M. ASHRY Benha University, Faculty of Agriculture, Agriculture Microbiology Department, Qalubia 13736 (EG)
  • Mostafa A. ABDEL-MAKSOUD King Saud University, College of Science, Botany and Microbiology Department, P.O. Box 2455, Riyadh 11451 (SA)
  • Abdul MALIK King Saud University, College of Pharmacy, Department of Pharmaceutics (SA)
  • Bushra H. KIANI Worcester Polytechnic Institute, Department of Biology and Biotechnology, Worcester, Massachuesetts, 01609 (US)
  • Sherif S. SALEH Horticulture Research Institute, Agricultural Research Center, Giza, Egypt (EG)

DOI:

https://doi.org/10.15835/nbha52313769

Keywords:

biocontrol agents, Erwinia amylovora, nanoparticles, sesame oil

Abstract

Erwinia amylovora, the primary cause of fire blight, is thought to be one of the most difficult crop diseases to eradicate. The study aimed to evaluate the performance of essential sesame oil (SO) and silver nanoparticle synthesized by sesame oil SO-AgNPs against E. amylovora. Using GC-MS, it was found that essential sesame oil contains the main component of the oil was sesamin, asarninin, heptane, c-Sitosterol, anethol, and trimethyl -6- ((s) - 4-methylcyclohexan – 3 – en-1-yl)) tetrahydro -2H-pyran. The diluted sesame oil (SO) was used as a reducing agent in synthesis of AgNPs in aqueous solution. By using UV-Visible spectrophotometry (UV–Vis), Dynamic Light Scattering (DLS), High Resolution Transmission Electron Microscopy (HRTEM), and Fourier Transformer InfraRed (FTIR) analysis, the produced Ag NPs were studied. The average particle size of the spherical Ag NPs was determined to be 54.98 nm using data from HRTEM and DLS. SO-Ag NPs (20 µg/ml) showed a promising antibacterial against E. amylovora, producing a 22.9 mm Zone of Inhibition (ZOI) against E. amylovora, followed by SO-Ag NPs (10 ug/ml) that gave 18.2 mm ZOI., compared to gentamicin that produced 13.2 mm ZOI. The most effective inducers were SO-Ag NPs at 10 ug/ml, which decreased the percentage of disease severity by 27.5 and increased the percentage of protection against disease infection by 68.39%. SO-Ag NPs was the most effective inducers which decreased the contents of Malonaldehyde (MDA) and H2O2 by 41.3% and 77.1%. Applying SO-Ag NPs or SO lowered the level of malondialdehyde (MDA) and hydrogen peroxide (H2O2) and improved the fruit set and yield in infected trees. We could assume that, to prevent E. amylovora fire blight disease in pears, SO-Ag NPs, SO are safe, effective, and environmentally friendly alternatives to conventional antibiotics. 

References

Abbas MM, Ismael WH, Mahfouz AY, Daigham GE, Attia MS (2024). Efficacy of endophytic bacteria as promising inducers for enhancing the immune responses in tomato plants and managing Rhizoctonia root-rot disease. Scientific Reports 14(1):1331. https://doi.org/10.1038/s41598-023-51000-8

Abd Alhakim A, Hashem A, Abdelaziz AM, Attia MS (2022). Impact of plant growth promoting fungi on biochemical defense performance of tomato under fusarial infection. Egyptian Journal of Chemistry 65(13):291-301. https://doi.org/10.21608/EJCHEM.2022.124008.5532

Abdelaziz AM, El-Wakil DA, Hashem AH, Al-Askar AA, AbdElgawad H, Attia MS (2023). Efficient role of endophytic Aspergillus terreus in biocontrol of Rhizoctonia solani causing damping-off disease of Phaseolus vulgaris and Vicia faba. Microorganisms 11(6):1487. https://doi.org/10.3390/microorganisms11061487

Abdelhalim MAK, Mady MM, Ghannam MM (2012). Physical properties of different gold nanoparticles: ultraviolet-visible and fluorescence measurements. Journal of Nanomedicine and Nanotechology 3(3):178-194. https://doi.org/10.4172/2157-7439.1000133

Anju V, Busi S, Ranganathan S, Ampasala DR, Kumar S, Suchiang K, Kumavath R, Dyavaiah M (2021). Sesamin and sesamolin rescues Caenorhabditis elegans from Pseudomonas aeruginosa infection through the attenuation of quorum sensing regulated virulence factors. Microbial Pathogenesis 155:104912. https://doi.org/10.1016/j.micpath.2021.104912

Artiga-Artigas M, Montoliu-Boneu J, Salvia-Trujillo L, Martín-Belloso O (2019). Factors affecting the formation of highly concentrated emulsions and nanoemulsions. Colloids and Surfaces A: Physicochemical and Engineering Aspects 578:123577. https://doi.org/10.1016/j.colsurfa.2019.123577

Attia MS, El-Sayyad GS, Saleh SS, Balabel NM, El-Batal AI (2019). Spirulina platensis-polysaccharides promoted green silver nanoparticles production using gamma radiation to suppress the expansion of pear fire blight-producing Erwinia amylovora. Journal of Cluster Science 30: 919-935. https://doi.org/10.1007/s10876-019-01550-7

Bahadou SA, Ouijja A, Karfach A, Tahiri A, Lahlali R (2018). New potential bacterial antagonists for the biocontrol of fire blight disease (Erwinia amylovora) in Morocco. Microbial Pathogenesis 117:7-15. https://doi.org/10.1016/j.micpath.2018.02.011

Cai L, Chen J, Liu Z, Wang H, Yang H, Ding W (2018). Magnesium oxide nanoparticles: effective agricultural antibacterial agent against Ralstonia solanacearum. Frontiers in Microbiology 9:790. https://doi.org/10.3389/fmicb.2018.00790

Chandrakar V, Tapadia K, Wag G (2022). Green fabrication of silver nanoparticles via Ipomea carnea latex extract: Antibacterial activity. Journal of the Indian Chemical Society 99(9):100648. https://doi.org/10.1016/j.jics.2022.100648

Cheraghi E, Ameri E, Moheb A (2016). Continuous biosorption of Cd (II) ions from aqueous solutions by sesame waste: thermodynamics and fixed-bed column studies. Desalination and Water Treatment 57(15):6936-6949. https://doi.org/10.1080/19443994.2015.1012744

Chopra B, Dhingra AK (2021). Natural products: A lead for drug discovery and development. Phytotherapy Research 35(9):4660-4702. https://doi.org/10.1002/ptr.7099

Ciriolo MR, Palamara AT, Incerpi S, Lafavia E, Bue MC, De Vito P, ... Rotilio G (1997). Loss of GSH, oxidative stress, and decrease of intracellular pH as sequential steps in viral infection. Journal of Biological Chemistry 272(5):2700-2708. https://doi.org/10.1074/jbc.272.5.2700

Costa FS, Silva PP, Morais CL, Arantes TD, Milan EP, Theodoro RC, Lima KM (2016). Attenuated total reflection Fourier transform-infrared (ATR-FTIR) spectroscopy as a new technology for discrimination between Cryptococcus neoformans and Cryptococcus gattii. Analytical Methods 8(39):7107-7115. https://doi.org/10.1039/C6AY01893A

Das SS, Verma P, Kar S, Singh SK (2020). Quercetin-loaded nanomedicine as oncotherapy. Nanomedicine for Bioactives: Healthcare Applications 155-183. https://doi.org/10.1007/9

Dsouza FP, Dinesh S, Sharma S (2024). Understanding the intricacies of microbial biofilm formation and its endurance in chronic infections: a key to advancing biofilm-targeted therapeutic strategies. Archives of Microbiology 206(2): 85. https://doi.org/10.1007/s00203-023-03802-7

Elad Y, Pertot I (2014). Climate change impacts on plant pathogens and plant diseases. Journal of Crop Improvement 28(1):99-139. https://doi.org/10.1080/15427528.2014.865412

Elakraa AA, Salem SS, El-Sayyad GS, Attia MS (2022). Cefotaxime incorporated bimetallic silver-selenium nanoparticles: promising antimicrobial synergism, antibiofilm activity, and bacterial membrane leakage reaction mechanism. RSC Advances 12(41):26603-26619. https://doi.org/10.1039/D2RA04717A

El-Batal AI, El-Sayyad GS, Al-Shammari BM, Abdelaziz AM, Nofel MM, Gobara M, ... Attia MS (2023). Protective role of iron oxide nanocomposites on disease index, and biochemical resistance indicators against Fusarium oxysporum induced-cucumber wilt disease: In vitro, and in vivo studies. Microbial Pathogenesis 180:106131. https://doi.org/10.1016/j.micpath.2023.106131

El-Batal AI, Ismail MA, Amin MA, El-Sayyad GS, Osman MS (2023). Selenium nanoparticles induce growth and physiological tolerance of wastewater‑stressed carrot plants. Biologia 78(9):2339-2355. https://doi.org/10.1007/s11756-023-01401-x

Elkhodary BH, Attia MS, El-Sayyad GS, Salem MS (2023). Effectiveness of bimetallic ZnO-B2O3 nanoparticles produced by Streptomyces gancidicus as prospective antifungal agents and therapeutic nutrients to enhance pea plant immunity against damping off-causing Pythium irregulare: in vivo and in vitro investigations. Biomass Conversion and Biorefinery 1-24. https://doi.org/10.1007/s13399-023-04913-3

Fouda HM, Saied E, Abdelmouty ES, Osman MS (2024). Ameliorative role of copper nanoparticle in alleviating salt-induced oxidative stress in fenugreek (Trigonella foenum-graecum L.) plants. Biocatalysis and Agricultural Biotechnology 57: 103095. https://doi.org/10.1016/j.bcab.2024.103095

Hashem AH, Saied E, Amin BH, Alotibi FO, Al-Askar AA, Arishi AA, ... Elbahnasawy MA (2022). Antifungal activity of biosynthesized silver nanoparticles (AgNPs) against aspergilli causing aspergillosis: Ultrastructure Study. Journal of Functional Biomaterials 13(4):242. https://doi.org/10.3390/jfb13040242

Haynes E, Ramwell C, Griffiths T, Walker D, Smith J (2020). Review of antibiotic use in crops, associated risk of antimicrobial resistance and research gaps. Report to Department for Environment, Food and Rural Affairs (Defra) & The Food Standards Agency (FSA), 1-83. https://doi.org/10.46756/sci.fsa.vnq132

Hu Z, Richter H, Sparovek G, Schnug E (2004). Physiological and biochemical effects of rare earth elements on plants and their agricultural significance: a review. Journal of Plant Nutrition 27(1):183-220. https://doi.org/10.1081/PLN-120027555

Jalill RDA (2018). Chemical analysis and anticancer effects of Juniperus polycarpos and oak gall plants extracts. Research Journal of Pharmacy and Technology 11(6): 2372-2387. https://doi.org/10.5958/0974-360X.2018.00440.7

Khan H, Ali F, Khan NM, Shah A, Ur Rahman S (2016). GC-MS analysis of fixed oil from Nelumbo nucifera Gaertn seeds: evaluation of antimicrobial, antileishmanial and urease inhibitory activities. Journal of the Chemical Society of Pakistan 38(6).

Kini AS, Prema K, Pai SN (2024). Early stage black pepper leaf disease prediction based on transfer learning using ConvNets. Scientific Reports 14(1): 1404. https://doi.org/10.1038/s41598-024-51884-0

Kumar S, Aeron A, Pandey P, Maheshwari DK (2011). Ecofriendly management of charcoal rot and Fusarium wilt diseases in sesame (Sesamum indicum L.). Bacteria in Agrobiology: Crop Ecosystems 387-405. https://doi.org/10.1007/978-3-642-18357-7_14

Lashin I, Hasanin M, Hassan SA, Hashem AH (2023). Green biosynthesis of zinc and selenium oxide nanoparticles using callus extract of Ziziphus spina-christi: Characterization, antimicrobial, and antioxidant activity. Biomass Conversion and Biorefinery 13(11):10133-10146. https://doi.org/10.1007/s13399-021-01873-4

Lesnichaya M, Perfileva A, Nozhkina O, Gazizova A, Graskova I (2022). Synthesis, toxicity evaluation and determination of possible mechanisms of antimicrobial effect of arabinogalactane-capped selenium nanoparticles. Journal of Trace Elements in Medicine and Biology 69:126904. https://doi.org/10.1016/j.jtemb.2021.126904

M Fahmy N (2020). Isolation and characterization of Streptomyces sp. NMF76 with potential antimicrobial activity from mangrove sediment, Red Sea, Egypt. Egyptian Journal of Aquatic Biology and Fisheries 24(6):479-495. https://doi.org/10.21608/EJABF.2020.117578

Medhioub I, Cheffi M, Tounsi S, Triki MA (2022). Study of Bacillus velezensis OEE1 potentialities in the biocontrol against Erwinia amylovora, causal agent of fire blight disease of rosaceous plants. Biological Control 167:104842. https://doi.org/10.1016/j.biocontrol.2022.104842

Mohamed MA, Hamed A, Kotb ANAE, Kamel MS (2021). GC/MS analyses of avocado and sesame fixed oils. SSRN Electronic Journal 6(4):721-725. http://dx.doi.org/10.2139/ssrn.3776629

Mukherjee S, Choudhuri M (1983). Implications of water stress‐induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiologia Plantarum 58(2):166-170. https://doi.org/10.1111/j.1399-3054.1983.tb04162

Mulyono N (2010). Identification of chemical constituents in stone dammar extracts and their potencies as antibacterial agents. Prosiding Seminar Nasional Universitas Terbuka. Retrieved 2012 May 19 from: http://www.pustaka.ut.ac.id/dev25/pdfprosiding2/fmipa201020.pdf

Munne-Bosch S, Penuelas J (2003). Photo-and antioxidative protection, and a role for salicylic acid during drought and recovery in field-grown Phillyrea angustifolia plants. Planta 217: 758-766.

Mushtaq A, Hanif MA, Ayub MA, Bhatti IA, Jilani MI (2020). Sesame. Medicinal Plants of South Asia. Elsevier: 601-615. https://doi.org/10.1007/s00425-003-1037-0

Narayanasamy P, Narayanasamy P (2013). Mechanisms of action of bacterial biological control agents. In: Biological Management of Diseases of Crops: Volume 1: Characteristics of Biological Control Agents: 295-429. https://doi.org/10.1007/978-94-007-6380

Negi DS, Negi N, Kumar A, Matsunami K, Schulz S, Jones PG (2013). (±)-Asarinin. Acta Crystallographica Section C: Crystal Structure Communications 69(1):87-89. https://doi.org/10.1107/S0108270112049657

Nyongesa BO, Were BAI, Gudu S, Dangasuk OG, Onkware AO (2013). Genetic diversity in cultivated sesame (Sesamum indicum L.) and related wild species in East Africa. Journal of Crop Science and Biotechnology 16:9-15. https://doi.org/10.1007/s12892-012-0114-y

Passari AK, Chandra P, Leo VV, Mishra VK, Kumar B, Singh BP (2017). Production of potent antimicrobial compounds from Streptomyces cyaneofuscatus associated with fresh water sediment. Frontiers in Microbiology 8. https://doi.org/10.3389/fmicb.2017.00068

Prajapati SK, Jain D, Parveen S, Maji S, Deb PK (2022). Nanodelivery of antioxidant herbal extracts, spices, and dietary constituents. Phytoantioxidants and Nanotherapeutics: 145-171. https://doi.org/10.1002/9781119811794.ch8

Rahuman HBH, Dhandapani R, Palanivel V, Thangavelu S, Paramasivam R, Muthupandian S (2021). Bioengineered phytomolecules-capped silver nanoparticles using Carissa carandas leaf extract to embed on to urinary catheter to combat UTI pathogens. PloS One 16(9):e0256748. https://doi.org/10.1371/journal.pone.0256748

Ratri PJ, Ayurini M, Khumaini K, Rohbiya A (2020). Clove oil extraction by steam distillation and utilization of clove buds waste as potential candidate for eco-friendly packaging. Jurnal Bahan Alam Terbarukan 9(1):47-54. https://doi.org/10.15294/jbat.v9i1.24935

Reshma M, Balachandran C, Arumughan C, Sunderasan A, Sukumaran D, Thomas S, Saritha S (2010). Extraction, separation and characterisation of sesame oil lignan for nutraceutical applications. Food Chemistry 120(4):1041-1046. https://doi.org/10.1016/j.foodchem.2009.11.047

Rocca JR, Nation JL, Strekowski L, Battiste MA (1992). Comparison of volatiles emitted by male Caribbean and Mexican fruit flies. Journal of Chemical Ecology 18:223-244. https://doi.org/10.1007/BF00993755

Roy R, Tiwari M, Donelli G, Tiwari V (2018). Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action. Virulence 9(1): 522-554. https://doi.org/10.1080/21505594.2017.1313372

Sagheer R, Nasibullah M, Iqbal N (2024). Recent trends in antimicrobial drug resistance and implications for the needs of microbial toxicology research. Antimicrobial Resistance in Agriculture and its Consequences, CRC Press 131-156. https://doi.org/10.1201/9781003269380

Saied E, Hashem AH, Ali OM, Selim S, Almuhayawi MS, Elbahnasawy MA (2022). Photocatalytic and antimicrobial activities of biosynthesized silver nanoparticles using Cytobacillus firmus. Life 12(9):1331. https://doi.org/10.3390/life12091331

Saied E, Salem SS, Al-Askar AA, Elkady FM, Arishi AA, Hashem AH (2022). Mycosynthesis of hematite (α-Fe2O3) nanoparticles using Aspergillus niger and their antimicrobial and photocatalytic activities. Bioengineering 9(8):397. https://doi.org/10.3390/life12091331

Salem SS, El-Belely EF, Niedbała G, Alnoman MM, Hassan SED, Eid AM, ... Fouda A (2020). Bactericidal and in-vitro cytotoxic efficacy of silver nanoparticles (Ag-NPs) fabricated by endophytic actinomycetes and their use as coating for the textile fabrics. Nanomaterials 10(10):2082. https://doi.org/10.3390/nano10102082

Sangave PC, Matkar NM, Suvarna V (2020). Antimicrobial activity of metallic nanoparticles using prokaryotic model organisms. Model Organisms to Study Biological Activities and Toxicity of Nanoparticles 59-81. https://doi.org/10.1007/978-981-15-1702

Sani I, Sule FA, Warra AA, Bello F, Fakai IM, Abdulhamid A (2013). Phytochemicals and mineral elements composition of white Sesamum indicum L. seed oil. International Journal of Traditional and Natural Medicines 2:118-130.

Sarheed NM, Jaffat HS (2022). Detection of chemical compounds and its antioxidant activity of aniseeds extract. AIP Conference Proceedings, AIP Publishing. https://doi.org/10.1063/5.0093811

Sarkar S, Jana AD, Samanta SK, Mostafa G (2007). Facile synthesis of silver nano particles with highly efficient anti-microbial property. Polyhedron 26(15):4419-4426. https://doi.org/10.1016/j.poly.2007.05.056

Sharaf MH, Abdelaziz AM, Kalaba MH, Radwan AA, Hashem AH (2022). Antimicrobial, antioxidant, cytotoxic activities and phytochemical analysis of fungal endophytes isolated from Ocimum basilicum. Applied Biochemistry and Biotechnology 1-19. https://doi.org/10.1007/s12010-021-03702.

Shehabeldine AM, Doghish AS, El-Dakroury WA, Hassanin MM, Al-Askar AA, AbdElgawad H, Hashem AH (2023). Antimicrobial, antibiofilm, and anticancer activities of Syzygium aromaticum essential oil nanoemulsion. Molecules 28(15):5812. https://doi.org/10.3390/molecules28155812

Srivastava S, Bist V, Srivastava S, Singh PC, Trivedi PK, Asif MH, Chauhan PS, Nautiyal CS (2016). Unraveling aspects of Bacillus amyloliquefaciens mediated enhanced production of rice under biotic stress of Rhizoctonia solani. Frontiers in Plant Science 7:587. https://doi.org/10.3389/fpls.2016.00587

Straßmann C, Krämer NC (2017). A categorization of virtual agent appearances and a qualitative study on age-related user preferences. Intelligent Virtual Agents: 17th International Conference, IVA 2017, Stockholm, Sweden, August 27-30, 2017, Proceedings 17. https://doi.org/10.1007/978-3-319-67401

Tiwari S, Mishra S, Misra DR, Upadhyay R (2016). Identification of new bioactive compounds from fruit of Abutilon indicum through GCMS analysis. Biology Forum—An International Journal 8:548-554.

Turek C, Stintzing FC (2013). Stability of essential oils: a review. Comprehensive Reviews in Food Science and Food Safety 12(1):40-53. https://doi.org/10.1111/1541-4337.12006

Vielma JR, Bonilla E, Chacín-Bonilla L, Mora M, Medina-Leendertz S, Bravo Y (2014). Effects of melatonin on oxidative stress, and resistance to bacterial, parasitic, and viral infections: a review. Acta Tropica 137:31-38. https://doi.org/10.1016/j.actatropica.2014.04.021

Wallis AE (2020). Investigating the Impacts of Antibiotics and Alternatives on the Sustainable Management, Distribution, and Spread of Fire Blight. Cornell University.

Warra A, Jonathan B, Ibrahim B, Adedara A (2016). GC-MS analysis of hexane extracts of two varieties of sesame (Sesamum indicum L.) seed oil. IJCPT 1(1):1-9.

Ye SF, Zhou YH, Sun Y, Zou LY, Yu JQ (2006). Cinnamic acid causes oxidative stress in cucumber roots, and promotes incidence of Fusarium wilt. Environmental and Experimental Botany 56(3):255-262. https://doi.org/10.1016/j.envexpbot.2005.02.010

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2024-08-20

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ATTIA, M. S., ABDALWAHAB, M. A., AWAD, A. A., KANDIL, E. K., ASHRY, N. M., ABDEL-MAKSOUD, M. A., MALIK, A., KIANI, B. H., & SALEH, S. S. (2024). Protective role of biosynthesized silver nanoparticles synthesized using sesame oil as biocontrol approach against Erwinia amylovora causing fire blight in pears (Pyrus communis L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(3), 13769. https://doi.org/10.15835/nbha52313769

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Vol. 52 No. 3 (2024)

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Research Articles
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DOI: 10.15835/nbha52313769

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