In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L.

Authors

  • Wesam G. SHAHROUR Al-Balqa Applied University, Faculty of Agricultural Technology, Department of Biotechnology, Al-Salt 19117 (JO)
  • Mohamad A. SHATNAWI Al-Balqa Applied University, Faculty of Agricultural Technology, Department of Biotechnology, Al-Salt 19117 (JO)
  • Mohammad AL-ALAWI Al-Balqa Applied University, Faculty of Agricultural Technology, Department of Plant Production and Protection, Al-Salt 19117 (JO)
  • Rida A. SHIBLI University of Jordan, Faculty of Agriculture, Department of Horticulture and Crop Sciences, Amman; The University of Jordan, Hamdi Mango Center for Scientific Research (HMCSR), Amman (JO)
  • Tamara S. ALQUDAH The University of Jordan, Hamdi Mango Center for Scientific Research (HMCSR), Amman (JO)
  • Majdi M. MAJDALAWI Al-Balqa Applied University, Zarqa University College, Zarqa (JO)
  • Abdel Rahman AL-TAWAHA Al Hussein bin Talal University, Department of Biological Sciences, P.O. Box 20, Maan (JO)
  • Ahmad ALJAMMAL Al-Balqa Applied University, Faculty of Agricultural Technology, Department of Biotechnology, Al-Salt 19117 (JO)

DOI:

https://doi.org/10.15835/nbha52113609

Keywords:

antimicrobial activity, Bemisia tabaci, Capparis spinosa L., in vitro multiplication, insecticidal activity, medical plant

Abstract

Caper (Capparis spinosa L.) is a medical plant grown in Jordan. Mass harvesting of caper plants from their origin environments caused a reduction of these germplasm. Therefore, an easy and consistent method for clonal proliferation and callus induction was established for this species. C. spinosa L. in vitro culture affected in MS medium provided by 0.5 mg/L BAP gave 5.9 microshoots/explant. Two months later MS medium supplemented with 2.0 mg/L NAA developed a maximum callus induction of 33.1 mm. Ex vitro, in vitro, and callus growth of C. spinosa L. using ethanolic and methanolic extracts were tested for their antimicrobial activity against different species of bacteria and fungi. Both ex-vitro and in vitro plants exhibited similar antimicrobial activity. Maximum ex vitro plant antibacterial activity was (23 mm ± 0.58 inhibition zone) against Staphylococcus epidermidi. In comparison, callus extracts gave the highest antibacterial activity against Bacillus cereus and Escherichia coli. Moreover, caper plant extracts showed different antifungal effects against the tested fungi species. Investigation of the data showed that ex-vitro extract exhibited maximum antifungal activity compared to in vitro plants. Additionally, exposed Bemisia tabaci 4th nymphal instar to C. spinosa L. extracts suffered mortality ranging from 2 to 28%.  In most instances, both ethanolic and methanolic extracts affected the survival of B. tabaci more than the control. The current study confirmed that C. spinosa L. has a wide range of antibacterial, antifungal, and insecticidal activity. 

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Published

2024-03-22

How to Cite

SHAHROUR, W. G., SHATNAWI, M. A., AL-ALAWI, M., SHIBLI, R. A., ALQUDAH, T. S., MAJDALAWI, M. M., AL-TAWAHA, A. R., & ALJAMMAL, A. (2024). In vitro multiplication, antimicrobial, and insecticidal activity of Capparis spinosa L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13609. https://doi.org/10.15835/nbha52113609

Issue

Vol. 52 No. 1 (2024)

Section

Research Articles
CITATION
DOI: 10.15835/nbha52113609

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Copyright (c) 2024 Wesam G. SHAHROUR, Mohamad A. SHATNAWI, Mohammad AL-ALAWI, Rida A. SHIBLI, Tamara S. ALQUDAH, Majdi M. MAJDALAWI, Abdel Rahman AL-TAWAHA, Ahmad ALJAMMAL

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