Unveiling the genetic landscape: Exploring the SSR-based genetic architecture and amino acid dissection of Gossypium barbadense and G. darwinii genomes

Authors

  • Allah DITTA Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Institute of Cotton Research, Anyang 455000, Henan; Nuclear Institute for Agriculture and Biology (NIAB), P.O. Box 128, Jhang Road, Faisalabad 38000, Punjab; NIAB-C, Pakistan Institute of Applied and Engineering Sciences (PIEAS), Nilor Islamabad (CN)
  • Zhongli ZHOU Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Institute of Cotton Research, Anyang 455000, Henan (CN)
  • Muhammad SHEHZAD Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Institute of Cotton Research, Anyang 455000, Henan (CN)
  • Xiaoyan CAI Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Institute of Cotton Research, Anyang 455000, Henan (CN)
  • Yanchao XU Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Institute of Cotton Research, Anyang 455000, Henan (CN)
  • Yuqing HOU Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Institute of Cotton Research, Anyang 455000, Henan (CN)
  • Fang LIU Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Institute of Cotton Research, Anyang 455000, Henan; National Nanfan Research Institute of Chinese Academy of Agriculture Sciences, Sanya (CN)
  • Kunbo WANG Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Institute of Cotton Research, Anyang 455000, Henan (CN)
  • Muhammad K.R. KHAN Nuclear Institute for Agriculture and Biology (NIAB), P.O. Box 128, Jhang Road, Faisalabad 38000, Punjab; NIAB-C, Pakistan Institute of Applied and Engineering Sciences (PIEAS), Nilor Islamabad (PK)
  • Kotb A. ATTIA King Saud University, College of Science, Department of Biochemistry, P.O. Box 2455, Riyadh 11451 (SA)
  • Yaser M. HAFEZ Kafr-el-sheikh University, Faculty of Agriculture, Plant Pathology and Biotechnology Laboratory, Agriculture Botany Department, EPCRS Excellence Center, 33516 (EG)
  • Arif A. MOHAMMED King Saud University, College of Science, Department of Biochemistry, P.O. Box 2455, Riyadh 11451 (SA)
  • Baohua WANG Nantong University, School of Life Sciences (CN)
  • Sajid FIAZ The University of Lahore, Institute of Molecular Biology and Biotechnology, 59540 Lahore (PK)
  • Baohong ZHANG East Carolina University, Department of Biology, Greenville, NC 27858 (US)

DOI:

https://doi.org/10.15835/nbha52313545

Keywords:

amino acid, fiber quality, genetic map, genetic variation, interspecific cross, wild cotton

Abstract

Genetic maps highlight the genome organization and structure but also provide the chance of tagging superior traits for crop improvement through marker-assisted selection. Amino acids are building blocks of proteins and perform crucial function in regulating the signaling of molecules involved in the development and growth of plants. Plant architecture also have an impact on crop productivity. In order to select elite cultivars for breeding and identification of favorable alleles and their functional properties, a deep understanding of genetic architecture and development of genetic map is essential. In present investigation, an interspecific cross of Gossypium barbadense XH-18 × G. darwinii 5-7 was made to develop a genetic map utilizing single sequence repeat markers for the dissection of amino acids involved in genetic architecture of G. barbadense and G. darwinii. We measured chromosomal distribution of 20 amino acids across the whole genome of both species. The map consists of 613 markers spread across all 26 chromosomes, covering 2371.4 cM of cotton genome with an average inter-marker distance of 9.35 cM. The marker number anchored on the chromosomes varied from 5 to 76 with an average of 23.57 on each chromosome. The Dt sub-genome had more markers (83.03%) than the At sub-genome (15.66%). Moreover, the longest chromosome was 143.387 cM, the shortest was 58.430 cM, and the average length was 91.207 cM. The Dt subgenome spans a greater genomic distance than the At subgenome. A sum of 21,035 genes were discovered, covering the complete genome of G. barbadense; G. darwinii and have been found to be involved in tRNA 3'-trailer cleavage, macromolecule modification, peptide deformylase activity, response to biotic stimulus and defense response. The minimum Glutamic acid (Glu), Histidine (His), and Lysine (Lys) were found on Chr.13 (0.00-17.74), Chr.02 (0.00-8.01), and Chr.06 (0.00-17.97), respectively found through chromosomal amino acid dissection. The genome-wide SSR interspecific genetic map of G. barbadense and G. darwinii is first of its kind, and studying chromosomal distribution of amino acids will set a landmark step to dissect the genome structure of G. darwinii.

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Published

2024-08-20

How to Cite

DITTA, A., ZHOU, Z., SHEHZAD, M., CAI, X., XU, Y., HOU, Y., LIU, F., WANG, K., KHAN, M. K., ATTIA, K. A., HAFEZ, Y. M., MOHAMMED, A. A., WANG, B., FIAZ, S., & ZHANG, B. (2024). Unveiling the genetic landscape: Exploring the SSR-based genetic architecture and amino acid dissection of Gossypium barbadense and G. darwinii genomes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(3), 13545. https://doi.org/10.15835/nbha52313545

Issue

Vol. 52 No. 3 (2024)

Section

Research Articles
CITATION
DOI: 10.15835/nbha52313545

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Copyright (c) 2024 Sajid Fiaz, Allah Ditta, Zhongli Zhou, Muhammad Shehzad, Xiaoyan Cai, Yanchao Xu, Yuqing Hou, Fang Liu, Kunbo Wang, Muhammad Kashif Riaz Khan, Kotb A. Attia, Asmaa M. Abushady, Baohua Wang, Baohong Zhang

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