Impact of biostimulants based amino acids and irrigation frequency on agro-physiological characteristics and productivity of broccoli plants


  • Hossam S. EL-BELTAGI King Faisal University, College of Agriculture and Food Sciences, Agricultural Biotechnology Department, Al-Ahsa 31982; Cairo University, Faculty of Agriculture, Biochemistry Department, Giza 12613 (SA)
  • Mohamed E. ABUARAB Cairo University, Faculty of Agriculture, Agricultural Engineering Department, Giza 12613 (EG)
  • Mahmoud A.M. FAHMY Beni-Suef University, Faculty of Agriculture, Horticulture Department, Beni-Suef 62517 (EG)
  • Suzy M. ABDELAZIZ Agricultural Research Centre, Horticulture Research Institute, Cross Pollinated Vegetable Research Department, 12611 (EG)
  • Sara G. ABDEL-HAKIM Cairo University, Faculty of Agriculture, Soil Science Department, Giza 12613 (EG)
  • Emad A. ABDELDAYM Cairo University, Faculty of Agriculture, Vegetable Crops Department, Giza, 12613 (EG)
  • Ghada A. TAWFIC Cairo University, Faculty of Agriculture, Vegetable Crops Department, Giza, 12613 (EG)



amino acids, biostimulants, Brassica oleracea, irrigation times, yield quantity and quality


Abstract scientists are concerned about the utilizing of biostimulants-based amino acids in plant feeding and mitigating negative effects of water stress. As a result, two-year field experiments were conducted at Faculty of Agriculture's experimental station at Cairo University to study the effectiveness of liquid yeast waste (CMS) and Cargo amino acids (AAs) on the agro-physiological features of broccoli plants with different irrigation frequencies (every 5, 10, and 15 days). Compared with control plants, the foliar application of AAs and CMS significantly increased plant height, photosynthesis pigments, head weight, head diameter, head height, plant yield, and quality at different irrigation frequencies. Likewise, both treatments (CMS and AAs) significantly improved ascorbic acids by 16.65% and 15.95% and increased total phenol content by 24.10% and 36.60%, respectively, compared to control plants. Accordingly, the highest productivity was achieved for broccoli grown under irrigation every five days with the CMS bio-stimulant, where it was 3111.17 kg ha-1, while the lowest productivity was achieved for the control treatment in which no biostimulants were added and irrigation frequency every 15 days, and the productivity was 1376.22 kg ha-1. Plants irrigated every 15 days produced the highest levels of abscisic acid (ABA) and superoxide dismutase (SOD), followed by plants irrigated every 10 days and every 5 days. Exogenous application of amino acids bio-stimulants could be suggested to improve vegetative growth, biochemical characteristics, productivity, and nutritional value as well as to mitigate negative effects of water scarcity. 


Abdel-Mawgoud A, El-Bassiouny A, Ghoname A, Abou-Hussein S (2011). Foliar application of amino acids and micronutrients enhance performance of green bean crop under newly reclaimed land conditions. Australian Journal for Basic and Applied Sciences 5:51-55.

Abdelaziz ME, Atia MA, Abdelsattar M, Abdelaziz SM, Ibrahim TA, Abdeldaym EA (2021). Unravelling the role of Piriformospora indica in combating water deficiency by modulating physiological performance and chlorophyll metabolism-related genes in Cucumis sativus. Horticulturae 7:399.

Abou El-Leel OF, Maraei RW, Aly AA (2019). Studying the response of nigella sativa plants to different fertilizers. Analele Universitatii din Oradea, Fascicula Biologie 26:14-20.

Acharya U, Thapa S, Sharma N (2015). Study on the growth, yield and soil nutrient status of broccoli under organic nutrient management. International Journal of Current Research 7:13029-13032.

Ahmad P (2016). Water stress and crop plants: a sustainable approach. John Wiley & Sons.

Al-Shammari AMA, Abood MA, Hamdi GJ (2018). Tecamin flower® foliar application to alleviate water deficit effects on growth, yield and water use efficiency of tomato. Journal of Agricultural Science XXIX:115-120.

Al-shammari AMA, Abood MA, Hamdi GJ (2020). Improvement in production, fruit quality and water use efficiency of three tomato cultivars by foliar application of Tecamin flower® under water deficit conditions. Journal of Central European Agriculture 21:379-385.

Allen RG (2000). Using the FAO-56 dual crop coefficient method over an irrigated region as part of an evapotranspiration intercomparison study. Journal of Hydrology 229:27-41.

Aly AA, Eliwa NE, Abd El Megid MH (2019). Improvement of growth, productivity and some chemical properties of hot pepper by foliar application of amino acids and yeast extract. Potravinarstvo 13(1):831-839.

Aly AA, Latif HH (2011). Differential effects of paclobutrazol on water stress alleviation through electrolyte leakage, phytohormones, reduced glutathione and lipid peroxidation in some wheat genotypes (Triticum aestivum L.) grown in-vitro. Romanian Biotechnological Letters 16(6):6710-6767.

Ardebili ZO, Moghadam ARL, Ardebili NO, Pashaie AR (2012). The induced physiological changes by foliar application of amino acids in Aloe vera L. plants. Plant Omics 5:279.

Bader BR, Abood MA, Aldulaimy SEH, Al-Mehmdya SMH, Hamdi GJ (2020). Effect of water deficit and foliar application of amino acids on growth and yield of eggplant irrigated by two drip systems under greenhouse conditions. Journal of Agricultural Science XXXI:131-138.

Beauchamp C, Fridovich I (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44:276-287.

Sadak MS, Bakry BA, Abdel-Razik TM, Hanafy RS (2023). Amino acids foliar application for maximizing growth, productivity and quality of peanut grown under sandy soil. Brazilian Journal of Biology 83.

Caverzan A, Casassola A, Brammer SP (2016). Reactive oxygen species and antioxidant enzymes involved in plant tolerance to stress. Abiotic and Biotic Stress in Plants-Recent Advances and Future Perspectives 17:463-480.

Chai Q, Gan Y, Turner NC, Zhang R-Z, Yang C, Niu Y, Siddique KH (2014). Water-saving innovations in Chinese agriculture. Advances in Agronomy 126:149-201.

De Tullio M, Arrigoni O (2004). Hopes, disillusions and more hopes from vitamin C. Cellular and Molecular Life Sciences CMLS 61:209-219.

Dewis J, Freitas F (1970). Physical and chemical methods of soil and water analysis. FAO soils Bulletin.

El-Bassiouny HMS, Abdallah M, El-Enany MAM, Sadak MS (2020). Nano-zinc oxide and arbuscular mycorrhiza effects on physiological and biochemical aspects of wheat cultivars under saline conditions. Pakistan Journal of Biological Sciences: PJBS 23:478-490.

El-Beltagi HS, Mohamed AA (2010). Changes in non-protein thiols, some antioxidant enzymes activity and ultrastructural alteration in radish plant (Raphanus sativus L.) grown under lead toxicity. Notulae Botanica Horti Agrobotanici Cluj-Napoca 38:76-85.

El-Beltagi HS, Ali MR, Ramadan KM, Anwar R, Shalaby TA, Rezk AA, El-Ganainy SM, Mahmoud SF, Alkafafy M, El-Mogy MM (2022). Exogenous postharvest application of calcium chloride and salicylic acid to maintain the quality of broccoli florets. Plants 11:1513.

El-Beltagi HS, El-Sayed SM, Abdelhamid AN, Hassan KM, Elshalakany WA, Nossier MI, Alabdallah NM, Al-Harbi NA, Al-Qahtani SM, Darwish DBE, et al. (2023). Potentiating biosynthesis of alkaloids and polyphenolic substances in Catharanthus roseus plant using κ-Carrageenan. Molecules 28:3642.

El-Shabasi M, Mohamed S, Mahfouz S (2005). Effect of foliar spray with amino acids on growth, yield and chemical composition of garlic plants. In: The 6th Arabian Conference for Horticulture. Ismailia, Egypt.

Enchalew B, Gebre S, Rabo M, Hindaye B, Kedir M, Musa Y, Shafi A (2016). Effect of deficit irrigation on water productivity of onion (Allium cepa l.) under drip irrigation. Irrigation & Drainage Systems Engineering 5:2.

FAO (2009). Food and Agriculture Organization of the United Nations. Retrieved 2023 October 28 from:

Gonzalez C, Zheng Y, Lovatt C (2008). Properly timed foliar fertilization can and should result in a yield benefit and net increase in grower income. In; VI International Symposium on Mineral Nutrition of Fruit Crops 868. pp 273-286.

Haghighi M, Barzegar Sadeghabad A, Abolghasemi R (2022). Effect of exogenous amino acids application on the biochemical, antioxidant, and nutritional value of some leafy cabbage cultivars. Scientific Reports 12:17720.

Haghighi M, Saadat S, Abbey L (2020). Effect of exogenous amino acids application on growth and nutritional value of cabbage under drought stress. Scientia Horticulturae 272:109561.

Haliński ŁP, Stepnowski P (2016). Short‐term water deficit changes cuticular sterol profile in the eggplant (Solanum melongena). Chemistry & Biodiversity 13:719-726.

Hancock RD, Viola R (2005). Biosynthesis and catabolism of L-ascorbic acid in plants. Critical Reviews in Plant Sciences 24:167-188.

Hildebrandt TM (2018). Synthesis versus degradation: directions of amino acid metabolism during Arabidopsis abiotic stress response. Plant Molecular Biology 98:121-135.

Hildebrandt TM, Nesi AN, Araújo WL, Braun H-P (2015). Amino acid catabolism in plants. Molecular Plant 8:1563-1579.

Howell T, Cuenca R, Solomon K (1990). Crop yield response. management of farm irrigation systems. Trans. ASAE Monograph Chap S. USA.

Islam M, Mollah AMD, Kaium A, Amin R, Sarkar MD (2014). Performance of different mulch materials on growth and yield of broccoli. Journal of Experimental Biosciences 5:43-48.

Jackson, M. (1967). Soil chemical analysis prentice. Hall of India Private Limited, New Delhi, pp 498.

Kim W, Iizumi T, Nishimori M (2019). Global patterns of crop production losses associated with droughts and floods from 1984 to 2009. In: 99th American Meteorological Society Annual Meeting. AMS.

Klute A, Page A (1986). Methods of soil analysis. American Society of Agronomy. Agronomy Monograph 9.

Koukounaras A, Tsouvaltzis P, Siomos AS (2013). Effect of root and foliar application of amino acids on the growth and yield of greenhouse tomato in different fertilization levels. Journal of Food, Agriculture & Environment 11:644-648.

Kumari A, Patel N, Mishra A (2018). Response of drip irrigated broccoli (Brassica oleracea var. italica) in different irrigation levels and frequencies at field level. Journal of Applied and Natural Science 10:12-16.

Lichtenthaler HK, Wellburn AR (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Portland Press Ltd.

Lordwin G, Tayal Z, Abineet R (2007). Yield, irrigation production efficiency and economic return of broccoli (Brassica oleracea var. italica) under different irrigation methods and schedules. Ethiopian Journal of Science and Technology 4:47-60.

Mahmoud AWM, Rashad HM, Esmail SE, Alsamadany H, Abdeldaym EA (2023). Application of silicon, zinc, and zeolite nanoparticles—a tool to enhance drought stress tolerance in coriander plants for better growth performance and productivity. Plants 12:2838.

Mehanna H, Hussein M, Gafaar N (2013). Using of growth regulators for improving water use of canola under water deficit. Middle East Journal of Applied Sciences 3:161-168.

Mobini M, Khoshgoftarmanesh A, Ghasemi S (2014). The effect of partial replacement of nitrate with arginine, histidine, and a mixture of amino acids extracted from blood powder on yield and nitrate accumulation in onion bulb. Scientia Horticulturae 176:232-237.

Mohamed AA, El-Beltagi HS, Rashed MM (2009). Cadmium stress induced change in some hydrolytic enzymes, free radical formation and ultrastructural disorders in radish plant. Electronic Journal of Environmental, Agricultural and Food Chemistry 8:969-983.

Mohammadipour N, Souri MK (2019). Effects of different levels of glycine in the nutrient solution on the growth, nutrient composition, and antioxidant activity of coriander (Coriandrum sativum L.). Acta Agrobotanica 72.

Moschen S, Di Rienzo JA, Higgins J, Tohge T, Watanabe M, González S, … Hopp HE (2017). Integration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.). Plant Molecular Biology 94:549-564.

Nora L, Dalmazo GO, Nora FR, Rombaldi CV (2012). Controlled water stress to improve fruit and vegetable postharvest quality. Water Stress 25:59-72.

Polle A, Otter T, Mehnejakobs B (1994). Effect of magnesium‐deficiency on antioxidative systems in needles of Norway spruce [Picea abies (L.) Karst.] grown with different ratios of nitrate and ammonium as nitrogen sources. New Phytologist 128:621-628.

Ramadan KMA, El-Beltagi HS, El-Mageed TAA, Saudy HS, Al-Otaibi HH, Mahmoud MAA (2023). The changes in various physio-biochemical parameters and yield traits of faba bean due to humic acid plus 6-benzylaminopurine application under deficit irrigation. Agronomy 13:1227.

Rodrigues R, Pizetta S, Hott M, Reis E, Teixeira A (2013). Desenvolvimento inicial de brócolis em diferentes disponibilidades hídricas. Enciclopédia Biosfera 9.

Sadak MS (2016). Physiological role of signal molecules in improving plant tolerance under abiotic stress. International Journal of ChemTech Research 9:46-60.

Sahebi M, Hanafi MM, Siti Nor Akmar A, Rafii MY, Azizi P, Tengoua FF, Nurul Mayzaitul Azwa J, Shabanimofrad M (2015). Importance of silicon and mechanisms of biosilica formation in plants. BioMed Research International, 15:1-16.

Saini RK, Yu J-W, Song M-H, Ahn H-Y, Lee J-H, Keum Y-S, Lee J-H (2022). Profiling of redox-active lipophilic constituents in leaf mustard (Brassica juncea (L.) Czern.) cultivars using LC-MS and GC-MS. Antioxidants 11:2464.

Sh Sadak M, Abdelhamid MT, Schmidhalter U (2015). Effect of foliar application of aminoacids on plant yield and some physiological parameters in bean plants irrigated with seawater. Acta Biológica Colombiana 20:141-152.

Shalom L, Samuels S, Zur N, Shlizerman L, Doron-Faigenboim A, Blumwald E, Sadka A (2014). Fruit load induces changes in global gene expression and in abscisic acid (ABA) and indole acetic acid (IAA) homeostasis in citrus buds. Journal of Experimental Botany 65:3029-3044.

Shekari G, Javanmardi J (2017). Effects of foliar application pure amino acid and amino acid containing fertilizer on broccoli (Brassica oleracea L. var. italica) transplant. Advances in Crop Science and Technology 5:280-287.

Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture 16:144-158.

Spann TM, Little HA (2010). Effect of Stimplex® crop biostimulant on drought tolerance of ‘Hamlin’sweet orange. In: Proceedings of the Florida State Horticultural Society 123:100-104.

Vomocil J (1957). Measurement of soil bulk density and penetrability: A review of methods. Advances in Agronomy 9:159-175.

Watts KT, Mijts BN, Lee PC, Manning AJ, Schmidt-Dannert C (2006). Discovery of a substrate selectivity switch in tyrosine ammonia-lyase, a member of the aromatic amino acid lyase family. Chemistry & Biology 13:1317-1326.

Weiland M, Mancuso S, Baluska F (2015). Signalling via glutamate and GLRs in Arabidopsis thaliana. Functional Plant Biology 43:1-25.

Wenneck GS, Saath R, Rezende R, Andrean AFBA, Santi DC (2021). Resposta agronômica de couve-flor à adição de silício ao solo sob estresse hídrico. Pesquisa Agropecuária Tropical 51.

Xie Z, Song R, Shao H, Song F, Xu H, Lu Y (2015). Silicon improves maize photosynthesis in saline-alkaline soils. The Scientific World Journal 2015:245072.

Zhang H, Birch J, Xie C, Yang H, Bekhit AE-D. (2019). Optimization of ultrasound assisted extraction method for phytochemical compounds and in-vitro antioxidant activity of New Zealand and China Asparagus cultivars (officinalis L.) roots extracts. Food chemistry 294:276-284.



How to Cite

EL-BELTAGI, H. S., ABUARAB, M. E., FAHMY, M. A., ABDELAZIZ, S. M., ABDEL-HAKIM, S. G., ABDELDAYM, E. A., & TAWFIC, G. A. (2023). Impact of biostimulants based amino acids and irrigation frequency on agro-physiological characteristics and productivity of broccoli plants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(4), 13454.



Research Articles
DOI: 10.15835/nbha51413454

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