Genetic diversity assessment and in vitro propagation of some date palm (Phoenix dactylifera L.) varieties


  • Ahmed M. ABDELGHAFFAR Zagazig University, Faculty of Agriculture, Genetics Department, Zagazig 44511 (EG)
  • Rana M. ALSHEGAIHI Department of Biological Sciences, College of Science, University of Jeddah, 21493, Jeddah (SA)
  • Mariam A. ALKHATEEB Princess Nourah bint Abdulrahman University, College of Science, Department of Biology, P.O.BOX 84428, Riyadh 11671 (SA)
  • Rahma ALSHAMRANI Department of Biological Sciences, College of Science, King Abdulaziz University, Jeddah (SA)
  • Amani O. ABUZAID Department of Biological Sciences, College of Science, King Abdulaziz University, Jeddah (SA)
  • Said SOLIMAN Zagazig University, Faculty of Agriculture, Genetics Department, Zagazig 44511 (EG)
  • Tarek ISMAIL Zagazig University, Faculty of Agriculture, Genetics Department, Zagazig 44511 (EG)
  • Ahmed ELZOHERY Zagazig University, Faculty of Agriculture, Genetics Department, Zagazig 44511 (EG)
  • Diaa Abd EL-MONEIM Arish University, Faculty of Environmental and Agricultural Sciences, Department of Plant Production (Genetic Branch), El-Arish 45511 (EG)
  • Wessam F. FELEMBAN Department of Biological Sciences, College of Science, King Abdulaziz University, Jeddah; Immunology Unit, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah (SA)
  • Ameina S. ALMOSHADAK Department of Biological Sciences, College of Science, King Abdulaziz University, Jeddah (SA)
  • Abdallah A. HASSANIN Zagazig University, Faculty of Agriculture, Genetics Department, Zagazig 44511 (EG)



date palm, diversity assessment, genetic polymorphism, ISSR-PCR, micropropagation, molecular markers, regeneration


The evaluation of genetic diversity is crucial for breeders to develop strategies and improve the resilience, quality, and adaptability of the date palm. In this study, the genetic diversity of three date palm varieties was performed using ISSR-PCR molecular markers to determine its relationship with in vitro propagation response of these varieties. The molecular profiling was performed using ISSR-PCR. A total of 49 loci were produced by the PCR reactions, 38 of which were polymorphic while 11 were monomorphic. The level of polymorphism revealed by ISSR-PCR varied from 33.33% to 100%. The three date palm varieties were grouped into two clusters based on the results of cluster analyses that used morphological data and molecular profiles. Cluster I comprised the ‘Barhy’ variety and Cluster II included ‘Magdoul’ and ‘Amri’ varieties. The clustering analyses revealed the independence of the ‘Barhy’ variety in its characteristics from the other varieties based on either morphological or molecular data. The results of in vitro propagation showed that the ‘Amri’ variety exhibited the highest callus induction frequency (86.28%), callus weight (2.33 g), number of somatic embryos (9.32), number of shoots (14.62), number of roots (4.11), root length (4.63 cm), shoot length (13.61 cm) followed by ‘Magdoul’ and ‘Barhy’ varieties. The ‘Amri’ variety had the shortest callus induction period, at 23.26 days while the ‘Barhy’ variety exhibited the longest period of callus induction (28.55). It was deduced from the study that the ISSR marker reproduced trustworthy patterns of bands to determine the genetic diversity among different date palm varieties that are considered the cornerstone for the genetic improvement of date palms. The understanding of the relationship between genetic diversity and in vitro propagation response of date palm is essential for ensuring the long-term sustainability of its crop. This will facilitate better conservation and development of new date palm varieties that fulfil the needs of farmers and consumers.


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How to Cite

ABDELGHAFFAR, A. M., ALSHEGAIHI, R. M., ALKHATEEB, M. A., ALSHAMRANI, R., ABUZAID, A. O., SOLIMAN, S., ISMAIL, T., ELZOHERY, A., Abd EL-MONEIM, D., FELEMBAN, W. F., ALMOSHADAK, A. S., & HASSANIN , A. A. (2023). Genetic diversity assessment and in vitro propagation of some date palm (Phoenix dactylifera L.) varieties. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(4), 13449.



Research Articles
DOI: 10.15835/nbha51413449

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