Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva

Authors

  • Mostafa EL-SHEEKH Tanta University, Faculty of Science, Botany Department, Tanta 31527 (EG)
  • Samha DEWIDAR Tanta University, Faculty of Science, Botany Department, Tanta 31527 (EG)
  • Azza HAMAD Tanta University, Faculty of Science, Botany Department, Tanta 31527 (EG)
  • Abdelghafar M. ABU-ELSAOUD Imam Muhammad bin Saud Islamic University (IMSIU), College of Science, Department of Biology, Riyadh 11623; Suez Canal University, Faculty of Science, Department of Botany and Microbiology, Ismailia, 41522 (EG)
  • Maha ALHARBI Princess Nourah bint Abdulrahman University, College of Science, Department of Biology, P.O. Box 84428, Riyadh 11671 (SA)
  • Amr ELKELISH Imam Muhammad bin Saud Islamic University (IMSIU), College of Science, Department of Biology, Riyadh 11623; Suez Canal University, Faculty of Science, Department of Botany and Microbiology, Ismailia, 41522 (EG)

DOI:

https://doi.org/10.15835/nbha52113426

Keywords:

antioxidant enzymes, Dunaliella parva, photosynthesis, salinity

Abstract

The growth of the marine green alga Dunaliella parva was studied and optimized under different salinity levels of NaCl (0.5, 1, 2, 2.5, and 3.5 M). The growth was monitored by cell number pigment content (Chl. a, Chl. b, and carotenoids). The grown alga, under the optimal conditions, was exposed to different stresses (nitrogen, phosphorus starvation, and salinity either singly or combined. Under nitrogen and phosphorus starvation, either singly or combined, the growth rate and the metabolic activities were decreased. Under salt stress (2.5 M NaCl) combined with N starvation and heavy metals stress, glycerol production increased, while glycerol synthesis decreased under salt stress of 1 M NaCl and P starvation. Also, free radicals (total antioxidant, reducing power, DPPH, and Lipid peroxidation), pigment content, and activity of antioxidant enzymes were recorded. D. parva grown under salinity level (2.5 M NaCl) combined with nutrient starvation correlated with more efficient enzymatic antioxidant activity accumulation. This study strongly suggested that the induction of antioxidant defense was one component of the tolerance mechanism of D. parva to salinity, as evidenced by its growth behavior.

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Published

2024-03-11

How to Cite

EL-SHEEKH, M., DEWIDAR, S., HAMAD, A., ABU-ELSAOUD, A. M., ALHARBI, M., & ELKELISH, A. (2024). Effect of salinity, nitrogen and phosphorus stresses on growth and photosynthetic activity of the marine microalga Dunaliella parva. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13426. https://doi.org/10.15835/nbha52113426

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DOI: 10.15835/nbha52113426