Proteomic alterations in various plant tissues of maize under induced chromium stress

Authors

  • Asma ZULFIQAR Institute of Botany, University of the Punjab, Lahore (PK)
  • Rushaankkauser KIANI Institute of Botany, University of the Punjab, Lahore (PK)
  • Muneera A. SALEH Taif University, College of Science, Department of Biology, P.O. Box 11099, Taif 21944 (SA)
  • Ibtisam M. ALSUDAYS Qassim University, College of Science and Arts, Department of Biology, Unaizah 56452 (SA)
  • Sami Asir AL-ROBAI Al-Baha University, Faculty of Science, Department of Biology, Al-Baha (SA)
  • Khalid H. ALAMER King Abdulaziz University, Faculty of Science and Arts, Biological Sciences Department, Rabigh 21911 (SA)
  • Rehana SARDAR Institute of Botany, University of the Punjab, Lahore (PK)
  • Kamran ASHRAF State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237 (CN)
  • Faisal MEHMOOD The University of Lahore, Department of Environmental Sciences, Lahore 54590 (PK)
  • Qamar uz ZAMAN The University of Lahore, Department of Chemistry, Lahore 54590 (PK)

DOI:

https://doi.org/10.15835/nbha51413323

Keywords:

chromium, gel electrophoresis, growth, maize, protein bands

Abstract

Heavy metal contamination is becoming a major cause of pollution in the environment, impacting humans, animals, and plants directly. Because of its widespread use in the tanning industry, chromium (Cr) regarded as a highly dangerous environmental toxin. The goal of this study was to investigate growth and proteins changes in different plant tissues (leaves, shoots, and seeds) of two maize cultivars (NMH-360 and DKC 61-42) under the stress of Cr (0, 50, and 150 ppm Cr). Sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) was used to observe plant proteome modification in response to Cr stress. Results revealed that at 150 ppm, both maize cultivars showed a decrease in plant growth attributes. Furthermore, it was noticed that the plant proteome changed in response to Cr stress in leaf and shoot tissues. A few proteins were up-regulated (70 kDa in C1 variety), while others were down-regulated (154, 140, 115, 80, 70 and 53 kDa in C1 cultivar shoots while 154, 65, 60, 17 kDa bands in shoots of C2 cultivar). Some protein bands were induced at 50 ppm, some at 150 ppm, and yet others at both concentrations. The findings of this study could aid in the selection of Cr-tolerant plant cultivars as well as the design of new protein biomarkers that can be utilized as a monitoring tool in heavy metal stress responses.

Author Biographies

Asma ZULFIQAR, Institute of Botany, University of the Punjab, Lahore

 

 

Rushaankkauser KIANI, Institute of Botany, University of the Punjab, Lahore

 

 

Muneera A. SALEH, Taif University, College of Science, Department of Biology, P.O. Box 11099, Taif 21944

 

 

Ibtisam M. ALSUDAYS, Qassim University, College of Science and Arts, Department of Biology, Unaizah 56452

 

 

Sami Asir AL-ROBAI, Al-Baha University, Faculty of Science, Department of Biology, Al-Baha

 

 

Khalid H. ALAMER, King Abdulaziz University, Faculty of Science and Arts, Biological Sciences Department, Rabigh 21911

 

 

Rehana SARDAR, Institute of Botany, University of the Punjab, Lahore

 

 

Kamran ASHRAF, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237

 

 

Faisal MEHMOOD, The University of Lahore, Department of Environmental Sciences, Lahore 54590

 

 

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Published

2023-11-17

How to Cite

ZULFIQAR, A., KIANI, R., SALEH, M. A., ALSUDAYS, I. M., AL-ROBAI, S. A., ALAMER, K. H., SARDAR, R., ASHRAF, K., MEHMOOD, F., & uz ZAMAN , Q. (2023). Proteomic alterations in various plant tissues of maize under induced chromium stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(4), 13323. https://doi.org/10.15835/nbha51413323

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Research Articles
CITATION
DOI: 10.15835/nbha51413323