Biocontrol of Fusarium wilt disease in pepper plant by plant growth promoting Penicillium expansum and Trichoderma harzianum

Authors

  • Amer M. ABDELAZIZ Al-Azhar University, Faculty of Science, Botany and Microbiology Department, Cairo (EG)
  • Mohamed H. SHARAF Al-Azhar University, Faculty of Science, Botany and Microbiology Department, Cairo (EG)
  • Amr H. HASHEM Al-Azhar University, Faculty of Science, Botany and Microbiology Department, Cairo (EG)
  • Abdulaziz A. AL-ASKAR King Saud University, Faculty of Science, Department of Botany and Microbiology, Riyadh (SA)
  • Samy A. MAREY King Saud University, Riyadh, 11451 (SA)
  • Fares A. MOHAMED Al-Azhar University, Faculty of Science, Botany and Microbiology Department, Cairo (EG)
  • Mohamed N. ABDELSTAR Al-Azhar University, Faculty of Science, Botany and Microbiology Department, Cairo (EG)
  • Mosad A. ZAKI Al-Azhar University, Faculty of Science, Botany and Microbiology Department, Cairo (EG)
  • Hamada ABDELGAWAD Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, 2020 Antwerp (BE)
  • Mohamed S. ATTIA Al-Azhar University, Faculty of Science, Botany and Microbiology Department, Cairo (EG)

DOI:

https://doi.org/10.15835/nbha51313302

Keywords:

Fusarium, biochemical defense, Trichoderma, Penicillium, primary metabolites, POD, PPO

Abstract

Plant growth promoting fungi (PGPF) were employed in the present study to biocontrol Fusarium wilt disease in pepper plants. Two of the five fungal isolates were chosen based on biochemical characteristics such as their production of hydrocyanic acid, siderophores, and IAA, phosphate solubilization, and in vitro antifungal activities. The most potent fungal isolates were identified as Penicillium expansum (P. expansum) and Trichoderma harzianum (T. harzianum). Using GC-MS, it was found that PGPF extracts contain compounds with antifungal activity, antioxidants, and plant growth stimulators. The combined effect of T. harzianum and P. expansum increased the protection against fusarial wilt by (76.74%), followed by T. harzianum by (50%), then P. expansum by (17.64%). Significant improvement because of using the mixture (T. harzianum and P. expansum) showed an increase in shoot length (59.4%), root length (129%), and number of leaves (52.6%). Chlorophyll A and B levels in infected plants were consistently raised by 28.71% and 67.58%, respectively; as a result of application the mixture (T. harzianum and P. expansum). Also, there was an increase in soluble proteins and carbohydrates in infected plants treated with (T. harzianum) by 25.42% and 31.78% over untreated infected plants, respectively. It could be recommended that the use of targeted PGPF strains, especially a mixture of T. harzianum and P. expansum could be commercially used as therapeutic nutrients against Fusarium wilt of pepper plants.

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Published

2023-09-26

How to Cite

ABDELAZIZ, A. M., SHARAF, M. H., HASHEM, A. H., AL-ASKAR, A. A., MAREY, S. A., MOHAMED, F. A., ABDELSTAR, M. N., ZAKI, M. A., ABDELGAWAD, H., & ATTIA, M. S. (2023). Biocontrol of Fusarium wilt disease in pepper plant by plant growth promoting Penicillium expansum and Trichoderma harzianum. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(3), 13302. https://doi.org/10.15835/nbha51313302

Issue

Section

Research Articles
CITATION
DOI: 10.15835/nbha51313302

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