Nutraceutical and antioxidant potential of selected wild edible plants from the cold-arid desert of Ladakh, India

Authors

  • Zohra BATOOL Plant Sciences and Agrotechnology Division, CSIR Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, Jammu and Kashmir; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002 (IN)
  • Javaid F. LONE Plant Sciences and Agrotechnology Division, CSIR Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, Jammu and Kashmir; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002 (IN)
  • Kanwaljeet SINGH Department of Education in Science and Mathematics, Regional Institute of Education, Mysuru, 570006, National Council of Educational Research and Training, New Delhi (IN)
  • Sumeet GAIROLA Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002; Department of Botany and Microbiology, HNB Garhwal University, Srinagar, Garhwal 246174, Uttarakhand (IN)

DOI:

https://doi.org/10.15835/nbha52113286

Keywords:

antioxidants, carbohydrate, Ladakh, protein, vitamin C, wild edible plants

Abstract

Ladakh’s wild edible plant species (WEPs) provide a vital source of food, nutrition, and medicine to the region’s indigenous populations. However, WEPs’ nutritional and antioxidant properties are missing in the region. The current study examined the nutritional values, total phenolic, total flavonoid, and antioxidant capabilities of 11 WEPs from the cold-arid region of Ladakh, India. Lactucata tatarica had the greatest carbohydrate content (36.48 ± 0.15 mg g-1Dw) among 11 wild edible plant species.  Malva verticillata had a greater glucose concentration (292.74 ± 0.01 ng g-1Dw), and Urtica hyperborea had higher protein and vitamin C levels (38.07 ± 2.44 and 49.95 ± 1.18 mg g-1Dw, respectively). The plant samples collected were also shown to have high total phenolic, flavonoid, and antioxidant capabilities. Our findings revealed that these species should be promoted as a natural source of nutraceuticals, with the potential to supplement the diets of locals. To boost food availability in a location noted for its intense weather conditions and short vegetative period, an agroforestry system that promotes sustainable use and increases the output of these species is critical.

References

Abu Bakar MF, Mohamed M, Rahmat A, Fry J (2009). Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus). Food Chemistry 113(2):479-483. https://doi.org/10.1016/j.foodchem.2008.07.081

Alam MK, Rana ZH, Islam SN, Akhtaruzzaman M (2020). Comparative assessment of nutritional composition, polyphenol profile, antidiabetic and antioxidative properties of selected edible wild plant species of Bangladesh. Food Chemistry 320:126646. https://doi.org/10.1016/j.foodchem.2020.126646

Ali B, Rawal YK, Sidharth, Thayes C (2022). Nutritional composition, phytochemical screening, antioxidant, GC-MS, and FTIR analysis of methanolic extract of Prunus armeniaca. Arabian Journal of Medicinal and Aromatic Plants 8(3):149-169. https://doi.org/10.48347/IMIST.PRSM/ajmap-v8i3.33827

AliV, Khajuria M, Bhat R, Rashid A, Faiz S, Vyas D (2021). Comparative phytochemical analysis of Lepidium latifolium L. sprouts from Ladakh Himalayas suggest a novel combination of 2-propenyl and benzyl glucosinolate. LWT 138:110713. https://doi.org/10.1016/j.lwt.2020.110713

Ali Z, Yadav A, Stobdan T, Singh SB (2012). Traditional methods for storage of vegetables in cold arid region of Ladakh, India. Indian Journal of Traditional Knowledge 11(2):351-353.

Ammo K, Adhikari BS, Rawat GS (2012). Changing aspects of Traditional Healthcare System in Western Ladakh, India. Journal of Ethnopharmacology 143(2):621-630. https://doi.org/10.1016/j.jep.2012.07.017

AOAC (2019). Official methods of analysis, 21st ed. Association of Official Analytical Chemists (AOAC), Arlington, Virginia.

AvasthiAS, Bhatnagar M, Sarkar N, Kitchlu S, Ghosal S (2016). Bioassay guided screening, optimization and characterization of antioxidant compounds from high altitude wild edible plants of Ladakh. Journal of Food Science and Technology 53(8):3244-3252. https://doi.org/10.1007/s13197-016-2300-2

Ballabh B, Chaurasia OP (2007). Traditional medicinal plants of cold desert Ladakh-Used in treatment of cold, cough and fever. Journal of Ethnopharmacology 112(2):341-349. https://doi.org/10.1016/j.jep.2007.03.020

Ballabh B, Chaurasia OP, Ahmed Z (2007). Raw edible plants of cold desert Ladakh. Indian Journal of Traditional Knowledge 06(1):182-184.

Batool Z, Singh K, Gairola S (2023). Medicinal plants traditionally used in the health care practices by the indigenous communities of the Trans-Himalayan Region of Ladakh, India. Journal of Ethnopharmacology 317:116837. https://doi.org/10.1016/J.JEP.2023.116837

Benzie IFF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry 239(1):70-76. https://doi.org/10.1006/abio.1996.0292

Bhoyar MS, Mishra GP, Naik PK, Singh SB (2018). Evaluation of antioxidant capacities and total polyphenols in various edible parts of Capparis spinosa L. collected from Trans-Himalayas. Defence Life Science Journal 3(2):140. https://doi.org/10.14429/dlsj.3.12570

Boesi A (2014). Traditional knowledge of wild food plants in a few Tibetan communities. Journal of Ethnobiology and Ethnomedicine 10:75. https://doi.org/10.1186/1746-4269-10-75

Chanwitheesuk A, Teerawutgulrag A, Rakariyatham N (2005). Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food Chemistry 92(3):491-497. https://doi.org/10.1016/j.foodchem.2004.07.035

Dhar P, Tayade AB, Kumar J, Chaurasia OP, Srivastava RB, Singh SB (2013). Nutritional profile of phyto-cocktail from trans-Himalayan plants. PLoS One 8(12):e83008. https://doi.org/10.1371/journal.pone.0083008

Dorjey K (2015). Exploration of plant based traditional knowledge from Sham Region of Ladakh (J&K), India. Journal of Plant Development Sciences 7(5):429-433.

Dorjey K, Tamchos S, Kumar S (2012). Ethnobotanical observations in Trans-Himalayan Region of Ladakh. Journal of Plant Development Sciences 4(4):459-464.

Gairola S, Sharma J, Bedi YS (2014). A cross-cultural analysis of Jammu, Kashmir and Ladakh (India) medicinal plant use. Journal of Ethnopharmacology 155(2):925-986. https://doi.org/10.1016/j.jep.2014.06.029

Guleria S, Tiku AK, Singh G, Vyas D, Bhar DWajA (2011). Antioxidant activity and protective effect against plasmid DNA strand scission of leaf, bark, and heartwood extracts from Acacia catechu. Journal of Food Science 76(7):C959-C964. https://doi.org/10.1111/j.1750-3841.2011.02284.x

Gupta S, Jyothi Lakshmi A, Manjunath MN, Prakash J (2005). Analysis of nutrient and antinutrient content of underutilized green leafy vegetables. LWT 38(4):339-345. https://doi.org/10.1016/j.lwt.2004.06.012

Haq SM, Yaqoob U, Calixto ES, Rahman IU, Hashem A, Abdallah EF, Alakeel MA, Alqarawi AA, Abdalla M, Hassan M, Bussmann RW, Abbasi AM, Rahman SU, Ijaz F (2021). Plant resources utilization among different ethnic groups of Ladakh in trans-Himalayan region. Biology 10(9):827. https://doi.org/10.3390/biology10090827

Jacinto-AzevedoB, Valderrama N, Henríquez K, Aranda M, Aqueveque P (2021). Nutritional value and biological properties of Chilean wild and commercial edible mushrooms. Food Chemistry 356:129651. https://doi.org/10.1016/j.foodchem.2021.129651

Jiménez-González A, Quispe C, Bórquez J, Sepúlveda B, Riveros F, Areche C, Nagles E, García-Beltrán O, Simirgiotis MJ (2018). UHPLC-ESI-ORBITRAP-MS analysis of the native Mapuche medicinal plant palo negro (Leptocarpha rivularis DC.–Asteraceae) and evaluation of its antioxidant and cholinesterase inhibitory properties. Journal of Enzyme Inhibition and Medicinal Chemistry 33(1):936-944. https://doi.org/10.1080/14756366.2018.1466880

Kachroo U, Sapru BL, Dhar U (1977). Flora of Ladakh. An ecological and taxonomical appraisal. Bishen Singh Mahendra Pal Singh, New Delhi.

Kaul MK (1997). Medicinal plants of Kashmir and Ladakh: Temperate and cold arid Himalaya. Indus publishing, New Delhi.

Kaur T, Hussain K, Koul S, Vishwakarma R, Vyas D (2013). Evaluation of nutritional and antioxidant status of Lepidium latifolium Linn.: A novel phyto-food from Ladakh. PLoS One 8(8):e69112. https://doi.org/10.1371/journal.pone.0069112

Kobeasy MI, Abdel-Fatah OM, Abd El-Salam SM, Zahrat El-Ola MM (2011). Biochemical studies on Plantago major L. and Cyamopsis tetragonoloba L. International Journal of Biodiversity and Conservation 3:83-91.

Maikhuri RK, Parshwan DS, Kewlani P, Negi VS, Rawat S, Rawat LS (2021). Nutritional composition of seed kernel and oil of wild edible plant species from Western Himalaya, India. International Journal of Fruit Science 21(1):609-618. https://doi.org/10.1080/15538362.2021.1907009

Nirmala C, Shahar B, Dolma N, Santosh O (2022). Promising underutilized wild plants of cold desert Ladakh, India for nutritional security and health benefits. Applied Food Research 2(2):100145. https://doi.org/10.1016/j.afres.2022.100145

Pal Murugan M, Janifer Raj X, Phani Kumar G, GuptaS, Singh SB (2010). Phytofoods of Nubra valley, Ladakh - The cold desert. Indian Journal of Traditional Knowledge 9(2):303-308.

Polunin O, Stainton A (1985). Flowers of the Himalaya. Oxford University Press, New Delhi. https://doi.org/10.2307/2806554

Kumar GMP, Shiddamallayya N (2021). Nutritional and anti-nutritional analysis of wild edible plants in Hassan district of Karnataka, India. Indian Journal of Natural Products and Resources 12(2):281-290. https://doi.org/10.56042/ijnpr.v12i2.22944

Raj X, Bajpai P, Murugan M, Srivastava R (2012). Antioxidant activity of Urtica hyperborea from cold arid desert Ladakh. Madras Agricultural Journal 4(6):181-184.

Rana JC, Pradheep K, Chaurasia OP, Sood S, Sharma RM, Singh A, Negi R (2012). Genetic resources of wild edible plants and their uses among tribal communities of cold arid region of India. Genetic Resources and Crop Evolution 59(1):135-149. https://doi.org/10.1007/s10722-011-9765-7

Rana ZH, Alam MK, Akhtaruzzaman M (2019). Nutritional composition, total phenolic content, antioxidant and α-amylase inhibitory activities of different fractions of selected wild edible plants. Antioxidants 8(7):203. https://doi.org/10.3390/antiox8070203

Razak S, Afsar T, Al-Disi D, Almajwal A, Arshad M, Alyousef AA, Chowdary RA (2020). GCMS fingerprinting, in vitro pharmacological activities, and in vivo anti-inflammatory and hepatoprotective effect of selected edible herbs from Kashmir valley. Journal of King Saud University - Science 32(6):2868-2879. https://doi.org/10.1016/j.jksus.2020.07.011

Rezzan A, Ozan EE, Huseyin S, Oktay Y, Nimet B (2013). Phenolic components, antioxidant activity, and mineral analysis of Capparis spinosa L. African Journal of Biotechnology 12(47):6643-6649. https://doi.org/10.5897/ajb2013.13241

Samancioglu A, Sat IG, Yildirim E, Ercisli S, Jurikova T, Mlcek J (2016). Total phenolic and vitamin C content and antiradical activity evaluation of traditionally consumed wild edible vegetables from Turkey. Indian Journal of Traditional Knowledge 15(2):208-213.

Šamec D, Pavlović I, Radojčić Redovniković I, Salopek-Sondi B (2018). Comparative analysis of phytochemicals and activity of endogenous enzymes associated with their stability, bioavailability and food quality in five Brassicaceae sprouts. Food Chemistry 269:96-102. https://doi.org/10.1016/j.foodchem.2018.06.133

Sharma VK, DWivedi SK, Awasthi OP, Verma MK (2014). Variation in nutrient composition of sea buckthorn (Hippophae rhamnoides L.) leaves collected from different locations of Ladakh. Indian Journal of Horticulture 71(3):421-423.

Stewart RR (1916). The Flora of Ladak, Western Tibet. I. Discussion of the Flora. Bulletin of the Torrey Botanical Club 43(11):571. https://doi.org/10.2307/2479609

Stobdan T, Stanzin A, Dorjey A, Eli P, Thinles D, Tashi T, Chaurasia OP (2017). Vegetable production scenario in Trans-Himalayan Leh Ladakh Region, India. Defence Life Science Journal 3(1):85-92. https://doi.org/10.14429/dlsj.3.11661

Tag H, Tsering J, Hui PK, Gogoi BJ, Veer V (2014). Nutritional potential and traditional uses of high-altitude wild edible plants in eastern Himalayas, India. International Science Index 8(3):238-243.

Tayade AB, Dhar P, Sharma M, Chauhan RS, Chaurasia OP, Srivastava RB (2013). Antioxidant capacities, phenolic Contents, and GC/MS analysis of Rhodiola imbricata Edgew. Root extracts from Trans-Himalaya. Journal of Food Science 78(3):C402-C410. https://doi.org/10.1111/1750-3841.12054

Uniyal MR (1981). A preliminary study of medicinal plants from Suru valley in Ladakh. Bulletin of Medico-Ethno-Botanical Research 2:316-326.

Vishwakarma KL, Dubey V (2011). Nutritional analysis of indigenous wild edible herbs used in eastern Chhattisgarh, India. Emirates Journal of Food and Agriculture 23(6):554-560.

Xavier JR, Bajpai PK, Palm M (2011). Trans-Himalayan phytofoods – A rich source of antioxidants. Journal of Medicinal and Aromatic Plant Sciences 33(1):21-26.

Yemm EW, Wills AJ (1954). The estimation of carbohydrates in plant extracts by anthrone. The Biochemical Journal 57(3):508-514. https://doi.org/10.1042/bj0570508

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Published

2024-03-28

How to Cite

BATOOL, Z., LONE, J. F., SINGH, K., & GAIROLA, S. (2024). Nutraceutical and antioxidant potential of selected wild edible plants from the cold-arid desert of Ladakh, India. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13286. https://doi.org/10.15835/nbha52113286

Issue

Vol. 52 No. 1 (2024)

Section

Research Articles
CITATION
DOI: 10.15835/nbha52113286

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