Comprehensive assessment of genetic variation in native heterostylous primrose genotypes of Türkiye


  • Mehmet TÜTÜNCÜ Ondokuz Mayıs University, Faculty of Agriculture, Department of Horticulture, Samsun (TR)
  • Akife DALDA-SEKERCI Erciyes University, Faculty of Agriculture, Department of Horticulture, Kayseri (TR)
  • Fatma BULUT Erciyes University, Faculty of Agriculture, Department of Horticulture, Kayseri (TR)
  • Özhan ŞİMŞEK Erciyes University, Faculty of Agriculture, Department of Horticulture, Kayseri (TR)



ISSR, ornamental plants, Primula


Primula vulgaris L. is an essential species of ornamental plant value with attractive flowers distributed in humid and cold regions. It spreads around Giresun, Gümüşhane, Trabzon, and Rize in Türkiye’s Eastern Black Sea region. Through molecular and morphological markers, this study determined the genetic diversity and similarity levels of Primula genotypes sampled from different areas (Samsun, Trabzon, Ordu, and Giresun). Variation was determined regarding morphological characteristics, and that plant shape, flower, and leaf properties were highly variable. Molecular data support morphological features. Thirteen ISSR primers determined the dimensions of genetic diversity, and the genotypes’ similarity indexes were between 0.70 and 0.92. It is seen that there is no clustering in the two- and three-dimensional graphics created in line with the genetic parameters, and the genotypes are distributed. Also, plant characteristics were correlated with the ISSR data, and many DNA profiles were detected. This study has characterized Primula genotypes that naturally spread in the Black Sea region of Türkiye. It is a guiding study for transferring some observed properties to culture varieties.


Anşin R (1994). Tohumlu bitkiler (Gymnospermae). [Flowering plants (Gymnospermae)]. Karadeniz Teknik Üniversitesi Yayınevi, Trabzon.

Baasanmunkh, S, Kovtonyuk, NK, Oyuntsetseg, B, Tsegmed, Z, Han, IV, Choi, HJ (2020). Diversity and distribution of the genus Primula L. (Primulaceae) in Mongolia. Journal of Asia-Pacific Biodiversity 13(4):687-700.

Baytop T (1994). Türkçe bitki adları sözlüğü [Turkish plant names dictionary]. Türk Dil Kurumu Yayınları, Ankara.

Bruun HG (1932). Cytological studies in Primula with special reference to the relation between the karyology and taxonomy of the genus (Doctoral dissertation, Acta Universitatis Upsaliensis).

Cservenka J, Endre G, Mihalik E (2002). Phenotypic and genetic pattern of the populations of Primula veris L., Primula vulgaris Huds. and their hybrids (Primula x brevistyla DC.). In International Conference on Medicinal and Aromatic Plants. Possibilities and Limitations of Medicinal and Aromatic Plant 576:75-80.

Dice LR (1945) Measures of the amount of ecologic association between species. Ecology 26:297-302.

Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11-15.

Fico G, Rodondi G, Flamini G, Passarella D, Tomé F (2007). Comparative phytochemical and morphological analyses of three Italian Primula species. Phytochemistry 68(12):1683-1691.

Gültepe M, Uzuner U, Coşkunçelebi K, Beldüz AO, Terzi̇oğlu S (2010). Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turkish Journal of Botany 34(3):147-157.

Guner A, Aslan S, Ekim T, Vural M, Babac MT (2012). Türkiye bitkileri listesi (Damarlı Bitkiler) [Türkiye plants list (Vascular plants)]. Flora Araştırmaları Derneği ve Nezahat Gokyiğit Botanik Bahcesi Yayını, İstanbul.

Heidari EF, Rahimmalek M, Mohammadi S, Ehtemam MH (2016). Genetic structure and diversity of ajowan (Trachyspermum ammi) populations based on molecular, morphological markers, and volatile oil content. Industrial Crops and Products 92:186-196.

Kato J, Inari-ikeda M, Hayashi M, Amano J, Ohashi H, Mii M (2018). Primula. In: Van Huylenbroeck J (Ed). Ornamental crops. Springer, Cham, pp 627-647.

Khan S, Shaheen H, Mehmood A, Nasar S, Khan T (2022). Ethnobotanical and antibacterial study of Primula plants traditionally used in the indigenous communities of Western Himalaya, Pakistan. Saudi Journal of Biological Sciences.

Kovtonyuk NK, Goncharov AA (2009). Phylogenetic relationships in the genus Primula L. (Primulaceae) inferred from the ITS region sequences of nuclear rDNA. Russian Journal of Genetics 45(6):663-670.

Majid A, Hassan S, Hussain W, Khan A, Hassan A, Khan A, Rehman MU (2014). In vitro approaches of Primula vulgaris leaves and roots extraction against human pathogenic bacterial strains. World Applied Sciences Journal 30(5):575-580.

Manfield IW, Pavlov VK, Li J, Cook HE, Hummel F, Gilmartin PM (2005). Molecular characterization of DNA sequences from the Primula vulgaris S-locus. Journal of Experimental Botany 56(414):1177-1188.

Morozowska M (2004). Vegetative development, flowering, fruiting and saponin content in cultivated cowslip (Primula veris L.) plants. Herba Polonica 2(50).

Parveen I, Gafner S, Techen N, Murch SJ, Khan IA (2016). DNA barcoding for the identification of botanicals in herbal medicine and dietary supplements: strengths and limitations. Planta Medica 82(14):1225-1235.

Richards J (2003). Primula L. Second ed., Timber Press, Portland, Oregon, USA.

Rohlf FJ (2000). NTSYS 2.1: numerical taxonomic and multivariate analysis system. Exeter Software, New York.

Simsek O, Donmez D, Saridas M, Paydas-Kargi S, Aka Kaçar, Y (2020). Genetic relationship and polymorphism of Turkish myrtles (Myrtus communis L.) as revealed by inter simple sequence repeat (ISSR). Applied Ecology Environmental Research 18(1):1141-1149.

Smith WW, Fletcher HR (1949). The Genus Primula: Sections Cuneifolia, Floribundae, Parryi, and Auricula. Earth and Environmental Science Transactions of The Royal Society of Edinburgh 61(3):631-686.

Van Geert A, Van Rossum, F, Stiers I, Sierens T, Barker JH, Triest L (2006). Isolation and characterization of microsatellite loci in primrose (Primula vulgaris). Belgian Journal of Botany 261-264.

Yang HB, Kang WH, Nahm SH, Kang, BC (2015). Methods for developing molecular markers. In: Current technologies in plant molecular breeding. Springer, Dordrecht, pp 15-50.

Yayli N (2001). Triterpenoid saponin from Primula elatior subsp. meyeri. Journal of Asian Natural Products Research 3(4):347-352.

Zhang LB, Kadereit JW (2004). Classification of Primula sect. Auricula (Primulaceae) based on two molecular data sets (ITS, AFLPs), morphology, and geographical distribution. Botanical Journal of the Linnean Society 146(1):1-26.

Zhang LB, Zhang LB, Kadereit JW (2005). Typification and synonymization in Primula sect. Auricula (Primulaceae). Taxon 54(3):775-788.



How to Cite

TÜTÜNCÜ, M., DALDA-SEKERCI, A., BULUT, F., & ŞİMŞEK, Özhan. (2023). Comprehensive assessment of genetic variation in native heterostylous primrose genotypes of Türkiye. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(4), 13276.



Research Articles
DOI: 10.15835/nbha51413276