Mineral contents and antioxidant activities of Karakılçık durum wheat (Triticum turgidum subsp. durum (Desf.) Husn.) genotypes


  • Fatma Ruveyda ALKAN Field Crops Central Research Institute, Department of Biodiversity and Genetic Resources, Türkiye Seed Gene Bank, Ankara 06172 (TR)
  • Melehat AVCI BİRSİN Ankara University, Faculty of Agriculture, Department of Field Crops, Ankara 06110 (TR)




biodiversity, breeding, DPPH, genotypes, ICP-OES, nutrition


The importance of antioxidants and minerals in food has increased with the growing awareness of consumers in recent years. Türkiye is rich in genetic diversity for wheat as it is located at the intersection of three floristic regions and is part of the Fertile Crescent. In this study, the antioxidant activity of Karakılçık durum wheat (Triticum turgidum subsp. durum (Desf.) Husn.) populations from 12 different regions of Türkiye was determined using the DPPH method. In addition, the contents of mineral nutrients (Ca, Cu, Fe, Mg, Mn, Zn) were determined in an ICP-OES device with milled wheat. Karakılçık genotypes were sowed in a field using an augmented trial design consisting of five rows and six blocks, with each block containing five control varieties. The result of the study was that the landrace Karakılçık genotypes exhibited antioxidant activity varying between 14.7-40.2 μmol TE g-1. The Ca contents of landrace Karakılçık genotypes varied between 13.0-249.9 ppm, Cu contents 0.6-22.7 ppm, Fe contents 28.7-93.9 ppm, Mg contents 583.7-1194.0 ppm, Mn contents 31.4-87.7 ppm, and Zn contents 22.3-68.5 ppm. The results of the study reveal that Karakılçık landrace genotypes have large differences in antioxidant and mineral content, that these differences have the potential to be used in wheat breeding programs, and that wheat flour obtained from Karakılçık landrace genotypes is important.


Adom KK, Sorrells EM, Liu RH (2005). Phytochemicals and antioxidant activity of milled fractions of different wheat varieties. Journal of Agricultural and Food Chemistry 53:2297-2306. https://doi.org/10.1021/jf048456d

Anglani C (1998). Wheat minerals -A review. Plant Foods for Human Nutrition 52(2):177-186.

Beta T, Nam E, Dexter JE, Sapirstein HD (2005). Phenolic content and antioxidant activity of pearled wheat and roller-milled fractions. Cereal Chemistry 82(4):390-393. https://doi.org/10.1094/CC-82-0390

Braun HJ, Atlin G, Payne T (2010). Multi-location testing as a tool to identify plant response to global climate change. In: Reynolds MP (Ed). Climate change and crop production. London, UK, CAB International. pp 115-138

Briguglio M, Hrelia S, Malaguti M, Lombardi G, Riso P, Porrini M, Banfi G (2020). The central role of iron in human nutrition: From folk to contemporary medicine. Nutrients 12(6):1761. https://doi.org/10.3390/nu12061761

Brown KH, Wuehler SE, Peerson JM (2001). The importance of zinc in human nutrition and estimation of the global prevalence of zinc deficiency. Food and Nutrition Bulletin 22(2):113-125. https://doi.org/10.1177/156482650102200201

Corbo MR, Bevilacqua A, Petruzzi L, Casanova FP, Sinigaglia M (2014). Functional beverages: the emerging side of functional foods: commercial trends, research, and health implications. Comprehensive reviews in food science and food safety 13(6):1192-1206. https://doi.org/10.1111/1541-4337.12109

Duthie G, Crozier A (2000). Plant-derived phenolic antioxidants. Current Opinion in Lipidology 11:43-47. https://doi.org/10.1097/00075197-200011000-00006

Ekinci R, Ünal S (2002). Türkiye’nin farklı bölgelerinde üretilen değişik tipte unların mineral madde miktarları [Mineral contents of various flour types produced in different Region of Türkiye]. Pamukkale University Journal of Engineering Sciences 8(1):91-96.

Esposito F, Arlotti G, Bonifati AM, Napolitane A, Vitale D, Fogliano V (2005). Antioxidant activity and dietary fiber in durum wheat bran by products. Food Research International 38(10):1167-1173. https://doi.org/10.1016/j.foodres.2005.05.002

Gomez-Becerra HF, Erdem H, Yazıcı A, Tutuş Y, Torun B, Öztürk L, Çakmak İ (2010). Grain concentrations of protein and mineral nutrients in a large collection of spelt wheat grown under different environments. Journal of Cereal Science 52(3):342-349. https://doi.org/10.1016/j.jcs.2010.05.003

Gulcin İ (2020). Antioxidants and antioxidant methods: An updated overview. Archives of Toxicology 94(3):651-715. https://doi.org/10.1007/s00204-020-02689-3

Hekmatnia M, Safdari M, Ahmadi A, Monibi H (2022). National savings in freshwater consumption in Iran by virtual water imports (a case study on cereals). Water and Environment Journal 36(4):656-666. https://doi.org/10.1111/wej.12805

Hernandez-Espinosa N, Laddomada B, Payne T, Huerta-Espino J, Govindan V, Ammar K, Guzman C (2020). Nutritional quality characterization of a set of durum wheat landraces from Iran and Mexico. LWT 124:109-198. https://doi.org/10.1016/j.lwt.2020.109198

Huang D, Ou B, Prior RL (2005). The chemistry behind antioxidant capacity assay. Journal of Agricultural and Food Chemistry 53(6):1841-1856. https://doi.org/10.1021/jf030723c

Hussain A, Larsson H, Kuktaite R, Johansson E (2010). Mineral composition of organically grown wheat genotypes: contribution to daily minerals ıntake. International Journal of Environmental Research Public Health 7(9):3442-3456. https://doi.org/10.3390/ijerph7093442

Jones JB (2001). Laboratory guide for conducting soil tests and plant analysis. CRC press, Boca Raton.

Khodaei D, Javanmardi F, Khaneghah AM (2021). The global overview of the occurrence of mycotoxins in cereals: A three-year survey. Current Opinion in Food Science 39:36-42. https://doi.org/10.1016/j.cofs.2020.12.012

Kim KH, Tsa R, Yang R, Cui SW (2006). Phenolic acid profiles and antioxidant activities of wheat bran extracts and effect of hydrolysis conditions. Food Chemistry 95:466-473. https://doi.org/10.1016/j.foodchem.2005.01.032

Kumar J, Kumar N, Sati N, Hota PK (2020). Antioxidant properties of ethenyl indole: DPPH assay and TDDFT studies. New Journal of Chemistry 44(21):8960-8970. https://doi.org/10.1039/d0nj01317j

Li L, Shewry PR, Ward JL (2008). Phenolic acids in wheat varieties in the health grain diversity screen. Journal of Agricultural and Food Chemistry 56:9732-9739. https://doi.org/10.1021/jf801069s

Macdiarmid JI, Clark H, Whybrow S, De Ruiter H, McNeill GM (2018). Assessing national nutrition security: The UK reliance on imports to meet population energy and nutrient recommendations. PLoS One 13(2):e0192649. https://doi.org/10.1371/journal.pone.0192649

Mckenzie JD, Goldman JMR (2004). The Student Guide to MINITAB Release 14 + MINITAB Student Release 14 Statistical Software (Book + CD). Pearson Education, Boston, MA.

Moore J, Liu J, Zhou K, Yu LL (2006). Effects of genotype and environment on the antioxidant properties of hard winter wheat bran. Journal of Agricultural and Food Chemistry 54:5313-5322. https://doi.org/10.1021/jf060381l

Mpofu A, Sapirstein HD, Beta T (2006). Genotype and environmental variation in phenolic content, phenolic acid composition and antioxidant activity of hard spring wheat. Journal of Agricultural and Food Chemistry 54:1265-1270. https://doi.org/10.1021/jf052683d

Okarter N, Liu CS, Sorrells ME, Liu RH (2010). Phytochemical content and antioxidant activity of six diverse varieties of whole wheat. Food Chemistry 119:249-257. https://doi.org/10.1016/j.foodchem.2009.06.021

Serpen A, Gökmen V, Karagöz A, Köksel H (2008). Phytochemical quantification and total antioxidant capacities of emmer (Triticum dicoccon Schrank) and einkorn (Triticum monococcum L.) wheat landraces. Journal of Agricultural and Food Chemistry 56(16):7285-7292. https://doi.org/10.1021/jf8010855

Shahidi F, Naczk M (1995). Food phenolics: Sources, chemistry, effects, applications. Technomic Pub. Co., Lancaster, Pa (1th ed), USA.

Söderström L, Rosenblad A, Adolfsson ET, Bergkvist L (2017). Malnutrition is associated with increased mortality in older adults regardless of the cause of death. British Journal of Nutrition 117(4):532-540. https://doi.org/10.1017/S0007114517000435

Stefoska-Needham A, Beck E J, Johnson SK, Tapsell LC (2015). Sorghum: an underutilized cereal whole grain with the potential to assist in the prevention of chronic disease. Food Reviews International 31(4):401-437. https://doi.org/10.1080/87559129.2015.1022832

Topçu A, Saldamlı İ, Sağlam F (1995). Vitamins and minerals. Hacettepe University Publising, Ankara, Türkiye.

Vaher M, Matso K, Levandi T, Helmja K, Kaljurand M (2010). Phenolic compounds and the antioxidant activity of the bran, flour and whole grain of different wheat varieties. Procedia Chemistry 2:76-82. https://doi.org/10.1016/j.proche.2009.12.013

WWF (2016). Wheat Atlas of Turkey. Retrieved 2023 September 19 from: http://www.wwf.org.tr/?6140

Yiğit A (2019). Determination of Yield, Bread Making Quality and Antioxidant Properties of Wheat Genotypes Under Different Ecological Conditions. PhD Thesis, Aydın Adnan Menderes University, Aydın, Türkiye.

Yu L, Haley S, Perret J, Harris M (2004). Comparison of wheat flours grown at different locations for their antioxidant properties. Food Chemistry 86(1):11-16. https://doi.org/10.1016/j.foodchem.2003.08.037

Zhang Y, Song Q, Yan J, Tang J, Zhao R, Zhang Y, He Z, Zou C, Ortiz-Monasterio I (2010). Mineral element concentrations in grains of Chinese wheat cultivars. Euphytica 174(3):303-313. https://doi.org/10.1007/s10681-009-0082-6

Zhou K, Yu LL (2004). Antioxidant properties of bran extracts from Trego wheat grown at different location. Journal of Agricultural and Food Chemistry 52:1112-1117. https://doi.org/10.1021/jf030621m



How to Cite

ALKAN, F. R., & AVCI BİRSİN, M. (2023). Mineral contents and antioxidant activities of Karakılçık durum wheat (Triticum turgidum subsp. durum (Desf.) Husn.) genotypes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(4), 13233. https://doi.org/10.15835/nbha51413233



Research Articles
DOI: 10.15835/nbha51413233