In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe

Authors

  • Surapon SAENSOUK Mahasarakham University, Walai Rukhavej Botanical Research Institute, Mahasarakham 44150; Mahasarakham University, Diversity of Family Zingiberaceae and Vascular Plant for Its Applications Research Unit, Mahasarakham 44150 (TH)
  • Wipa YAOWACHAI Mahasarakham University, Faculty of Science, Department of Biology, Mahasarakham 44150 (TH)
  • Theeraphan CHUMROENPHAT Ubon Ratchathani Rajabhat University, Faculty of Thai Traditional and Alternative Medicine, Aesthetic Sciences and Health Program, Ubonratchathani 34000 (TH)
  • Sukanya NONTHALEE Horticultural Research Institute, Trang Horticultural Research Center, Trang 92150 (TH)
  • Piyaporn SAENSOUK Mahasarakham University, Faculty of Science, Department of Biology, Mahasarakham 44150; Mahasarakham University, Diversity of Family Zingiberaceae and Vascular Plant for Its Applications Research Unit, Mahasarakham 44150 (TH)

DOI:

https://doi.org/10.15835/nbha51413190

Keywords:

antioxidant, GC-MS, Kaempferia angustifolia, micropropagation, TFC, TPC

Abstract

Kaempferia angustifolia Roscoe is a medicinal plant in the family Zingiberaceae, with wild occurrence decreasing as a result of both natural and human threats. Tissue culture techniques provide an alternative method of propagation for mass production. Microshoots of K. angustifolia (1 cm in length) were cultured on MS medium supplemented with various concentrations of cytokinins (BA and Kinetin) and auxins (NAA and IAA) for 8 weeks. Phytochemical profiles were evaluated by total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ⁄ antioxidant power (FRAP) assay) and Gas chromatography–mass spectrometry (GC–MS) analysis. Maximum number of shoots was observed in medium supplemented with 2 mg/l BA plus 0.5 mg/l NAA (6.33 shoots/explant) and optimal rooting was induced in MS medium with 2 mg/l Kinetin plus 1 mg/l NAA (35.70 roots/explant). Highest numbers of shoots and roots were obtained when cultured on liquid MS medium supplemented with 2 mg/l BA, 2 mg/l Kinetin and 1 mg/l NAA (8.73 shoots/explant and 29.67 roots/explant, respectively). Regenerated plantlets of K. angustifolia were transferred to pots containing different types of plant materials under natural conditions for 8 weeks. Optimal survival rate was 100% when transplanting K. angustifolia to soil, sand, soil and sand (1:1) or soil and small pieces of rock (1:1) ratio. The methanol extract of leaves K. angustifolia from natural plants and in vitro derives plants showed a significantly higher amount of TFC and antioxidant activity. GC-MS analysis identified 52 phytochemical compounds in leaves of K. angustifolia.  This study may be helpful to increase the value of commercial production of K. angustifolia, pharmaceutical and medicinal purpose.

References

Ajayi GO, Akinsanya MA, Agbabiaka AT, Oyebanjo KS, Hungbo TD, Olagunju JA (2019). D-Limonene: a major bioactive constituent in Allium fistulosum identified by GC-MS analysis. The Journal of Phytopharmacology 8(5):257-59. https://doi.org/10.31254/phyto.2019.8509

Alabi OS, Koleoso OB, Abiala AM (2019). Antimicrobial screening and GC-MS analysis of bioactive compounds from strains of Pseudomonas aeruginosa isolated from poultry fecal littered soil in Ibadan, Nigeria. Journal of Pure and Applied Science 32(1):3347-57. https://doi.org/10.6084/m9.figshare.12278864

Alizah Z, Nurulaishah Y, Adilah A (2019). In vitro propagation of Curcuma aeruginosa Roxb in liquid culture. South Asian Research Journal of Biology and Applied Biosciences 1(3):87-89. https://doi.org/10.36346/SARJBAB.2019.v01i03.006

Al-Rekaby LS, Atiyah KM (2020). Antibacterial activities for root extracts of (goldenrods) Solidago canadensis L. treated by nano and bio fertilizer. Al-Qadisiyah Journal of Pure Science 25(4):1-14. https://doi.org/10.29350/qjps.2020.25.4.1201

Anbazhagan M, Balachandran B, Arumugam K, Nagar A (2015). In vitro propagation of Kaempferia galanga (L.)-an endangered medicinal plant. Journal of Phytology 15:63-69.

Ashmore SE (1997). Status report on the development and application of in vitro techniques for the conservation and use of plant genetic resources. International Plant Genetic Resources Institute, Rome.

Babri RA, Khokhar I, Mahmud S (2015). Antioxidant potential and iron chelating activity of some bioactive compounds. Journal of the Chemical Society of Pakistan 37(3):514-519.

Bhattacharya M, Sen A (2013). In vitro regeneration of pathogen free Kaempferia galanga L.

-a rare medicinal plant. Research in Plant Biology 3(3):24-30.

Chavan MJ, Wakte PS, Shinde DB (2010). Analgesic and anti-inflammatory activity of caryophyllene oxide from Annona squamosa L. Bark. Phytomedicine 17(2):149-51. https://doi.org/10.1016/j.phymed. 2009.05.016

Chong YH, Khalafalla MM, Bhatt A, Chan LK (2012). The effects of culture systems and explant incision on in vitro propagation of Curcuma zedoaria Rosc. Pertanika Tropical Agricultural Science 35(4):863-874.

Devi RB, Barkath TN, Vijayaraghavan P, Rejiniemon TS (2018). GC-MS analysis of phytochemical from Psidium guajava Linn. leaf extract and their in vitro antimicrobial activities. International Journal of Pharmacy and Biological Sciences 8(1):583-589.

Gopalakrishnan S, Vadivel E (2011). GC-MS analysis of some bioactive constituents of Mussaenda frondosa Linn. International Journal of Pharma and Bio Sciences 2(1):313-320.

Hachlafi NEL, Aanniz T, Menyiy NEl, Baaboua AEl, Omari NEl, Balahbib A, … Bouyahya A. (2021). In vitro and in vivo biological investigations of camphene and its mechanism insights: a review. Food Reviews International 2-28. https://doi.org/10.1080/87559129.2021.1936007

Haque SM, Ghosh B (2018). Micropropagation of Kaempferia angustifolia Roscoe-an aromatic, essential oil yielding, underutilized medicinal plant of Zingiberaceae family. Journal of Crop Science and Biotechnology 21(2):147-53. https://doi.org/10.1007/s12892-017-0051-0

Ibemhal A, Laishram JM, Dhananjoy C, Naorem B, Toijam R (2012). In vitro induction of multiple shoot and root from the rhizome of Kaempferia galanga L. An International Journal of Environment and Biodiversity 3(3):46-50.

Kalpana M, Anbazhagan M (2009). In vitro production of Kaempferia galanga L.-an endangered medicinal plant. Journal of Phytology 1(1):56-61.

Kang GQ, Duan WG, Lin GS, Yu YP, Wang XY, Lu SZ (2019). Synthesis of bioactive compounds from 3-Carene (II): synthesis, antifungal activity and 3D-QSAR study of (Z)- and (E)-3-caren-5-one oxime sulfonates. Molecules 24(3):1-14. https://doi.org/10.3390/molecules24030477

Khandaker MM, Rasdi MZMD, Naeimah NN, Mat N (2017). Effects of naphthalene acetic acid (NAA) on the plant growth and sugars effects on the cut flowers Mokara chark kuan orchid. Bioscience Journal 33(1):19-30. https://doi.org/10.14393/BJ-v33n1a2017-34908

Kochuthressia KP, Britto SJ, Jaseentha MO (2012). In vitro multiplication of Kaempferia galanga L. an endangered species. International Research Journal of Biotechnology 3(2):27-31.

Mao QQ, Xu XY, Cao SY, Gan RY, Corke H, Beta T, Li HB (2019). Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods 8(185):1-21. https://doi.org/10.3390%2Ffoods8060185

Mehrotra, S, Goel MK, Kukreja AK and Mishra BN (2007). Efficiency of liquid culture systems over conventional micropropagation: a progress towards commercialization. African Journal of Biotechnology 6(13):1484-1492.

Mohanty S, Panda M, Sahoo S, Nayak S (2011). Micropropagation of Zingiber rubens and assessment of genetic stability through RAPD and ISSR markers. Biologia Plantarum 55(1):16-20. https://doi.org/10.1007/s10535-011-0002-1

Mondello F, Bernardis FD, Girolamo A, Cassone A, Salvatore G (2006). In vivo activity of terpinen-4-ol, the main bioactive component of Melaleuca alternifolia Cheel (tea tree) oil against azole-susceptible and-resistant human pathogenic Candida species. BMC Infectious Diseases 6(158):1-8. https://doi.org/10.1186/1471-2334-6-158

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassay tobacco tissue culture. Physiologia Plantarum 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Nonthalee S, Maneechai S, Saensouk S, Saensouk P (2022). In vitro propagation, microrhizome induction, and evaluation of genetic variation by RAPD markers of Kaempferia siamensis Sirirugsa. Propagation of Ornamental Plants 22(1):11-22.

Nonthalee S, Maneechai S, Saensouk S, Saensouk P (2023). Comparative phytochemical profiling (GC-MS and HPLC) and evaluation of antioxidant activities of wild, in vitro cultured and greenhouse plants of Kaempferia grandifolia Saensouk and Jenjitt and Kaempferia siamensis Sirirugsa; rare plant species in Thailand. Pharmacognosy Magazine 19(1):1-12. https://doi.org/10.1177/09731296221145066

Parida R, Mohanty S, Kuanar A, Nayak S (2010). Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture. Electronic Journal of Biotechnology 13(4):1-8. https://doi.org/10.2225/vol13-issue4-fulltext-12

Park HY, Kim K, Ak G, Zengin G, Cziáky Z, Jekő J, Adaikalam K, Song K, Kim DH, Sivanesan I (2021). Establishment of a rapid micropropagation system for Kaempferia parviflora Wall. Ex Baker: phytochemical analysis of leaf extracts and evaluation of biological activities. Plants, 10(698):2-28. https://doi.org/10.3390/plants10040698

Pereira I, Severino P, Santos AC, Silva AM, Souto EB (2018). Linalool bioactive properties and potential applicability in drug delivery systems. Colloids and Surfaces B: Biointerfaces 171(1):566-578. https://doi.org/10.1016/j.colsurfb.2018.08.001

Rahman MM, Amin MN, Ahamed T, Ahmad S (2005). In vitro rapid propagation of black thorn (Kaempferia galanga L.): a rare medicinal and aromatic plant of Bangladesh. Journal of Biological Sciences 5(3):300-304. https://doi.org/10.3923/jbs.2005.300.304

Rahman ZA, Othman1 AN, Ghazalli MN, Adlan NAS (2022). Micropropagation of Kaempferia angustifolia Roscoe via direct regeneration. American Journal of Plant Sciences 13:734-43. https://doi.org/10.4236/ajps.2022.136049

Ren B, Zhu Y, Zhang J, Dong S, Liu P, Zhao B (2016). Effects of spraying exogenous hormone 6-benzyladenine (6-BA) after water logging on grain yield and growth of summer maize. Field Crops Research 188:96-04. https://doi.org/10.1016/j.fcr.2015.10.016

Saensouk P, Muangsan N, Saensouk S, Sirinajun P (2016). In vitro propagation of Kaempferia marginata Carey ex Roscoe, a native plant species to Thailand. The Journal of Animal and Plant Sciences 26(5):1405-1410.

Sahoo S, Parida R, Singh S, Padhy RN, Nayak S (2014). Evaluation of yield, quality and antioxidant activity of essential oil of in vitro propagated Kaempferia galanga Linn. Journal of Acute Disease 3(2):124-130. https://doi.org/10.1016/S2221-6189(14)60028-7

Shareef HK, Muhammed HJ, Hussein HM, Hameed IH (2016). Antibacterial effect of ginger (Zingiber officinale) Roscoe and bioactive chemical analysis using gas chromatography mass spectrum. Oriental Journal of Chemistry 32(2):817-37. https://doi.org/10.13005/ojc/320207

Shirin F, Kumar S, Mishra Y (2000). In vitro plantlet production system for Kaempferia galanga L., a rare Indian medicinal herb. Plant Cell, Tissue and Organ Culture 63:193-97. https://doi.org/10.1023/A:1010635920518

Siriamornpun S, Tangkhawanit E, Kaewseejan N (2016). Reducing retrogradation and lipid oxidation of normal and glutinous rice flours by adding mango peel powder. Food Chemistry 201:160-67. https://doi.org/10.1016/j.foodchem.2016.01.094

Stanly C, Keng CL (2007). Micropropagation of Curcuma zedoaria Roscoe and Zingiber zerumbet Smith. Biotechnology 6(4):555-560. https://doi.org/10.3923/biotech.2007.555.560

Suphrom N, Sonyota W, Insumronga K, Sawangsupa, P, Sutamuanga, P, Ingkaninanb, K (2017). GC-MS analysis and in vitro anti-androgenic activity of Kaempferia rotunda Linn. extract. Naresuan University Journal: Science and Technology 4(25):34-43.

Tang SW, Sukari MA, Neoh BK, Yeap YS, Abdul AB, Kifli N (2014). Phytochemicals from Kaempferia angustifolia Rosc. and their cytotoxic and antimicrobial activities. BioMed Research International 417674:1-6. https://doi.org/10.1155/2014/417674

Tang SW, Sukari MA, Rahmani M, Lajis NH, Ali AM (2011). A new abietene diterpene and other constituents from Kaempferia angustifolia Rosc. Molecules 16(4):3018-3028. https://doi.org/10.3390/molecules16043018

Thammapat P, Meeso N, Siriamornpun S (2015). Effects of NaCl and soaking temperature on the phenolic compounds, α-tocopherol, γ-oryzanol and fatty acids of glutinous rice. Food Chemistry 175:218-224. https://doi.org/10.1016/j.foodchem.2014.11.146

Vadassery J, Ritter C, Venus Y, Camehl I, Varma A, Shahollari B (2008). The role of auxins and cytokinins in the mutualistic interaction between Arabidopsis and Piriformospora indica. Molecular Plant-Microbe Interactions 21(10):1371-1383. https://doi.org/10.1094/MPMI-21-10-1371

Wang Y, Wang J, Shi B, Yu T, Qi J, Meyerowitz EM (2014). The stem cell niche in leaf axils is established by auxin and cytokinin in Arabidopsis. The Plant Cell 26(5):2055-2067. https://doi.org/10.1105/tpc.114.123083

Wanyo P, Kaewseejan N, Meeso N, Siriamornpun S (2016). Bioactive compounds and antioxidant properties of different solvent extracts derived from Thai rice by products. Applied Biological Chemistry 59:373-384. https://doi.org/10.1007/s13765-016-0173-8

Werner T, Motyka V, Strnad M, Schmulling T (2001). Regulation of plant growth by cytokinin. Proceedings of the National Academy of Sciences of the United States of America 98(18):10487104-92. https://doi.org/10.1073/pnas.171304098

Werner T, Schmulling T (2009). Cytokinin action in plant development. Current Opinion in Plant Biology 12(5):527-38. https://doi.org/10.1016/j.pbi.2009.07.002

Yaowachai W, Saensouk S, Saensouk P (2020). In vitro propagation and determination of total phenolic compounds, flavonoid contents and antioxidative activity of Globba globulifera Gagnep. Pharmacognosy Journal 12(6):1-8. https://doi.org/10.5530/pj.2020.12.236

Yeap YSY, Kassim NK, Ng RC, Ee GCL, Yazan LS, Musa KH (2017). Antioxidant properties of ginger (Kaempferia angustifolia Rosc.) and its chemical markers. International Journal of Food Properties 20:1158-1172. https://doi.org/10.1080/10942912.2017.1286508

Downloads

Published

2023-11-15

How to Cite

SAENSOUK, S., YAOWACHAI, W., CHUMROENPHAT, T., NONTHALEE, S., & SAENSOUK, P. (2023). In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(4), 13190. https://doi.org/10.15835/nbha51413190

Issue

Vol. 51 No. 4 (2023)

Section

Research Articles
CITATION
DOI: 10.15835/nbha51413190

License

Copyright (c) 2023 Surapon SAENSOUK, Wipa YAOWACHAI, Theeraphan CHUMROENPHAT, Sukanya NONTHALEE, Piyaporn SAENSOUK

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

License:

© Articles by the authors; licensee UASVM and SHST, Cluj-Napoca, Romania. Papers published in the journal Notulae Botanicae Horti Agrobotanici Cluj-Napoca are open access distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses).

Open Access Journal:

The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.

Most read articles by the same author(s)