Conservation of commercial quality and bioactive compounds of guava pieces by application of an alginate-acemannan coating


  • Edén A. LUNA-ZAPIÉN Juárez University of the State of Durango, Faculty of Chemical Sciences, Gómez Palacio-Avenida Article 123 S/N, Fraccionamiento Filadelfia, CP. 35010, Gómez Palacio, Durango (MX)
  • Rafael MINJARES-FUENTES Juárez University of the State of Durango, Faculty of Chemical Sciences, Gómez Palacio-Avenida Article 123 S/N, Fraccionamiento Filadelfia, CP. 35010, Gómez Palacio, Durango (MX)
  • Erick SIERRA-CAMPOS Juárez University of the State of Durango, Faculty of Chemical Sciences, Gómez Palacio-Avenida Article 123 S/N, Fraccionamiento Filadelfia, CP. 35010, Gómez Palacio, Durango (MX)
  • Jolanta E. MARSZALEK Autonomous University of Coahuila, Faculty of Biological Sciences, Torreón-Matamoros Km 7.5, CP. 27276 Torreón, Coahuila (MX)
  • Sergio I. BARRAZA-GUERRERO Antonio Narro Autonomous Agrarian University, Laguna Unit, Raúl López Sanchez, Valle Verde, CP. 27054 Torreon, Coahuila (MX)
  • Jorge A. MEZA-VELÁZQUEZ Juárez University of the State of Durango, Faculty of Chemical Sciences, Gómez Palacio-Avenida Article 123 S/N, Fraccionamiento Filadelfia, CP. 35010, Gómez Palacio, Durango (MX)



acemannan, edible coating, fresh-cut, guava, sodium alginate


The objective of this study was to evaluate the effect of an edible coating based on alginate and acemannan on the commercial quality and concentration of bioactive compounds of guava pieces stored in refrigeration. Pieces of guava were coated with an alginate film enriched with acemannan (A-Ac), with samples without cover used as a control. The fruit pieces with and without the A-Ac coating were stored for 12 days at 6 ± 2 °C. Analyses of colour, firmness, ethylene production, ascorbic acid, total polyphenols, antioxidant potential, and polyphenol profile were performed at 0, 3, 6, 9, and 12 days of storage. In addition, a sensory analysis was carried out after nine days of refrigeration. The coated guava pieces retained firmness at 2.8 N, while in the uncoated pieces, firmness decreased to 1.3 N. Similarly, the coated pieces showed better colour retention (ΔE<9) and lower ethylene emission during storage. Also, the content of ascorbic acid, total polyphenols, antioxidant capacity, and the concentration of individually identified polyphenols were higher in the pieces of guava coated with the A-Ac film. In addition, coated guava pieces, stored for nine days in refrigeration, showed greater sensory acceptability compared to uncoated samples. Therefore, applying the A-Ac cover could be a good alternative for conserving sensory attributes and bioactive compounds of guava pieces stored at low temperatures.


Acevedo CA, López DA, Tapia MJ, Enrione J, Skurtys O, Pedreschi F, … Osorio F (2012). Using RGB Image processing for designing an alginate edible film. Food and Bioprocess Technology 5(5):1511-1520.

Ahmed MJ, Singh Z, Khan AS (2009). Postharvest Aloe vera gel-coating modulates fruit ripening and quality of ‘Arctic Snow’ nectarine kept in ambient and cold storage. International Journal of Food Science & Technology 44(5):1024-1033.

Alkaabi S, Sobti B, Mudgil P, Hasan F, Ali A, Nazir A (2022). Lemongrass essential oil and aloe vera gel based antimicrobial coatings for date fruits. Applied Food Research 2(1):100127.

Azarakhsh N, Osman A, Ghazali HM, Tan CP, Mohd Adzahan N (2014). Lemongrass essential oil incorporated into alginate-based edible coating for shelf-life extension and quality retention of fresh-cut pineapple. Postharvest Biology and Technology 88:1-7.

Babu M, Suriyakala MA, Kodiveri Muthukaliannan G (2012). Varietal impact on phytochemical contents and antioxidant properties of Musa acuminata (Banana). Journal of Pharmaceutical Sciences and Research 4:1950-1955.

Benítez S, Achaerandio I, Pujolà M, Sepulcre F (2015). Aloe vera as an alternative to traditional edible coatings used in fresh-cut fruits: A case of study with kiwifruit slices. LWT - Food Science and Technology 61(1): 184-193.

Blanco-Pascual N, Montero MP, Gómez-Guillén MC (2014). Antioxidant film development from unrefined extracts of brown seaweeds Laminaria digitata and Ascophyllum nodosum. Food Hydrocolloids 37:100-110.

Botondi R, Barone M, Grasso C (2021). A review into the effectiveness of ozone technology for improving the safety and preserving the quality of fresh-cut fruits and vegetables. Foods 10(4):748.

Bourbon AI, Pinheiro AC, Cerqueira MA, Rocha CMR, Avides MC, Quintas MAC, Vicente AA (2011). Physico-chemical characterization of chitosan-based edible films incorporating bioactive compounds of different molecular weight. Journal of Food Engineering 106(2):111-118.

Brasil I, Gomes, C, Puerta-Gomez A, Castell-Perez M, Moreira R (2012). Polysaccharide-based multilayered antimicrobial edible coating enhances quality of fresh-cut papaya. LWT - Food Science and Technology 47:39-45.

Castro-Acosta ML, Smith L, Miller RJ, McCarthy DI, Farrimond JA, Hall WL (2016). Drinks containing anthocyanin-rich blackcurrant extract decrease postprandial blood glucose, insulin and incretin concentrations. Journal of Nutrition and Biochemistry 38:154-161.

Chen J, Wu A, Yang M, Ge Y, Pristijono P, Li J, … Mi H (2021). Characterization of sodium alginate-based films incorporated with thymol for fresh-cut apple packaging. Food Control 126:108063.

Chokboribal J, Tachaboonyakiat W, Sangvanich P, Ruangpornvisuti V, Jettanacheawchankit S, Thunyakitpisal P (2015). Deacetylation affects the physical properties and bioactivity of acemannan, an extracted polysaccharide from Aloe vera. Carbohydrate Polymers 133:556-566.

Díaz-Mula HM, Serrano M, Valero D (2012). Alginate coatings preserve fruit quality and bioactive compounds during storage of sweet cherry fruit. Food and Bioprocess Technology 5(8):2990-2997.

Duncan SE, Chang H-H (2012). Implications of light energy on food quality and packaging selection. In: Henry J (Ed). Advances in Food and Nutrition Research. Academic Press, pp 25-73.

Ehteshami S, Dastjerdi AM, Ramezanian A, Etemadipoor R, Abdollahi F, Salari M, Shamili M (2022). Effects of edible alginate coating enriched with organic acids on quality of mango fruit during storage. Journal of Food Measurement and Characterization 16(1): 400-409.

Eim VS, Urrea D, Rosselló C, García-Pérez JV, Femenia A, Simal S (2013). Optimization of the drying process of carrot (Daucus carota v. Nantes) on the basis of quality criteria. Drying Technology 31(8):951-962.

Fakhouri FM, Martelli SM, Caon T, Velasco JI, Mei LHI (2015). Edible films and coatings based on starch/gelatin: Film properties and effect of coatings on quality of refrigerated Red Crimson grapes. Postharvest Biology and Technology 109:57-64.

Forato LA, de Britto D, de Rizzo JS, Gastaldi TA, Assis OBG (2015). Effect of cashew gum-carboxymethylcellulose edible coatings in extending the shelf-life of fresh and cut guavas. Food packaging and Shelf Life 5:68-74.

González-Centeno MR, Jourdes M, Femenia A, Simal S, Rosselló C, Teissedre P-L (2012). Proanthocyanidin composition and antioxidant potential of the stem winemaking byproducts from 10 different grape varieties (Vitis vinifera L.). Journal of Agricultural and Food Chemistry 60(48):11850-11858.

Guerreiro AC, Gago CML, Faleiro ML, Miguel MGC, Antunes MDC (2017). The effect of edible coatings on the nutritional quality of ‘Bravo de Esmolfe’ fresh-cut apple through shelf-life. LWT-Food Science and Technology 75:210-219.

Guo X, Mei N (2016). Aloe vera: A review of toxicity and adverse clinical effects. Journal of environmental science and health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews 34(2):77-96.

Hadi A, Nawab A, Alam F, Zehra K (2022). Alginate/aloe vera films reinforced with tragacanth gum. Food Chemistry: Molecular Sciences 4:100105.

Hambleton A, Perpiñan-Saiz N, Fabra MJ, Voilley A, Debeaufort F (2012). The Schroeder paradox or how the state of water affects the moisture transfer through edible films. Food Chemistry 132(4):1671-1678.

Hayama H, Shimada T, Fujii H, Ito A, Kashimura Y (2006). Ethylene-regulation of fruit softening and softening-related genes in peach. Journal of Experimental Botany 57(15):4071-4077.

Hu W, Sarengaowa Guan Y, Feng K (2022). Biosynthesis of phenolic compounds and antioxidant activity in fresh-cut fruits and vegetables [Review]. Frontiers in Microbiology 13.

Jideani AIO, Anyasi TA, McHau GRA, Udoro EO, Onipe OO (2017). Processing and preservation of fresh-cut fruit and vegetable products. Postharvest Handling 47.

Kanatt SR, Makwana SH (2020). Development of active, water-resistant carboxymethyl cellulose-poly vinyl alcohol-Aloe vera packaging film. Carbohydrate Polymers 227:115303.

Koh PC, Mohd Adzahan NA, Karim R, Rosli S (2017). Application of edible coatings and repetitive pulsed light for shelf life extension of fresh-cut cantaloupe ( Cucumis melo L. reticulatus cv. Glamour). Postharvest Biology and Technology 129:64-78.

Kumar S, Kumar R (2019). Role of acemannan O-acetyl group in murine radioprotection. Carbohydrate Polymers 207:460-470.

Li X, Li B, Li M, Fu X, Zhao X, Min D, … Zhang X (2022). Ethylene pretreatment induces phenolic biosynthesis of fresh-cut pitaya fruit by regulating ethylene signaling pathway. Postharvest Biology and Technology 192:112028.

Maan AA, Reiad Ahmed ZF, Iqbal Khan MK, Riaz A, Nazir A (2021). Aloe vera gel, an excellent base material for edible films and coatings. Trends in Food Science & Technology 116:329-341.

Maqbool M, Ali A, Ramachandran S, Smith DR, Alderson PG (2010). Control of postharvest anthracnose of banana using a new edible composite coating. Crop Protection 29(10):1136-1141.

Maringgal B, Hashim N, Mohamed Amin Tawakkal IS, Muda Mohamed MT (2020). Recent advance in edible coating and its effect on fresh/fresh-cut fruits quality. Trends in Food Science & Technology 96:253-267.

Martínez-Burgos WJ, Serra JL, Marsiglia FRM, Montoya P, Sarmiento-Vásquez Z, Marin O, … Paternina-Arboleda CD (2022). Aloe vera: From ancient knowledge to the patent and innovation landscape – A review. South African Journal of Botany 147:993-1006.

Mascaraque C, Aranda C, Ocón B, Monte MJ, Suárez MD, Zarzuelo A, … de Medina FS (2014). Rutin has intestinal antiinflammatory effects in the CD4+ CD62L+ T cell transfer model of colitis. Pharmacological Research 90:48-57.

Mendy TK, Misran A, Mahmud TMM, Ismail SI (2019). Application of Aloe vera coating delays ripening and extend the shelf life of papaya fruit. Scientia Horticulturae 246:769-776.

Minjares-Fuentes R, Rodríguez-González VM, González-Laredo RF, Eim V, González-Centeno MR, Femenia A (2017). Effect of different drying procedures on the bioactive polysaccharide acemannan from Aloe vera (Aloe barbadensis Miller). Carbohydrate Polymers 168:327-336.

Montone AMI, Malvano F, Pham PL, Cinquanta L, Capparelli R, Capuano F, Albanese D (2022). Alginate-based coatings charged with hydroxyapatite and quercetin for fresh-cut papaya shelf life. International Journal of Food Science and Technology 57(8):5307-5318.

Mostafidi M, Sanjabi MR, Shirkhan F, Zahedi MT (2020). A review of recent trends in the development of the microbial safety of fruits and vegetables. Trends in Food Science & Technology 103:321-332.

Nair MS, Saxena A, Kaur C (2018). Effect of chitosan and alginate-based coatings enriched with pomegranate peel extract to extend the postharvest quality of guava (Psidium guajava L.). Food Chemistry 240:245-252.

Nazir S, Wani IA (2022). Development and characterization of an antimicrobial edible film from basil seed (Ocimum basilicum L.) mucilage and sodium alginate. Biocatalysis and Agricultural Biotechnology 44:102450.

Nishimura M, Ohkawara T, Sato Y, Satoh H, Suzuki T, Ishiguro K, … Nishihira J (2016). Effectiveness of rutin-rich Tartary buckwheat (Fagopyrum tataricum Gaertn.) ‘Manten-Kirari’ in body weight reduction related to its antioxidant properties: A randomised, double-blind, placebo-controlled study. Journal of Functional Foods 26:460-469.

Nora CD, Müller CD-R, de Bona GS, Rios AdO, Hertz PF, Jablonski A, … Flôres SH (2014). Effect of processing on the stability of bioactive compounds from red guava (Psidium cattleyanum Sabine) and guabiju (Myrcianthes pungens). Journal of Food Composition and Analysis 34(1):18-25.

Olivas GI, Barbosa-Cánovas GV (2008). Alginate–calcium films: Water vapor permeability and mechanical properties as affected by plasticizer and relative humidity. LWT - Food Science and Technology 41(2):359-366.

Oliveira DS, Lobato AL, Ribeiro SM, Santana AM, Chaves JB, Pinheiro-Sant'Ana HM (2010). Carotenoids and vitamin C during handling and distribution of guava (Psidium guajava L.), mango (Mangifera indica L.), and papaya (Carica papaya L.) at commercial restaurants. Journal of Agricultural and Food Chemistry 58(10):6166-6172.

Oms-Oliu G, Rojas-Graü MA, González LA, Varela P, Soliva-Fortuny R, Hernando MIH, … Martín-Belloso O (2010). Recent approaches using chemical treatments to preserve quality of fresh-cut fruit: A review. Postharvest Biology and Technology 57(3):139-148.

Paula GA, Benevides NMB, Cunha AP, de Oliveira AV, Pinto AMB, Morais JPS, Azeredo HMC (2015). Development and characterization of edible films from mixtures of κ-carrageenan, ι-carrageenan, and alginate. Food Hydrocolloids 47:140-145.

Peretto G, Du W-X, Avena-Bustillos RJ, De J Berrios J, Sambo P, McHugh TH (2017). Electrostatic and conventional spraying of alginate-based edible coating with natural antimicrobials for preserving fresh strawberry quality. Food and Bioprocess Technology 10(1):165-174.

Qadri OS, Yousuf B, Srivastava AK (2015). Fresh-cut fruits and vegetables: Critical factors influencing microbiology and novel approaches to prevent microbial risks – A review. Cogent Food & Agriculture 1(1):1121606.

Radev R, Dimitrov G (2019). Water vapor permeability of edible films with different composition Scientific Works of University of Food Technologies 64(1):96-102.

Reyes-Avalos MC, Femenia A, Minjares-Fuentes R, Contreras-Esquivel JC, Aguilar-González CN, Esparza-Rivera JR, Meza-Velázquez JA (2016). Improvement of the quality and the shelf life of figs (Ficus carica) using an alginate–chitosan edible film. Food and Bioprocess Technology 9(12):2114-2124.

Reyes-Avalos MC, Minjares-Fuentes R, Femenia A, Contreras-Esquivel JC, Quintero-Ramos A, Esparza-Rivera JR, Meza-Velázquez JA (2019). Application of an alginate–chitosan edible film on figs (Ficus carica): Effect on bioactive compounds and antioxidant capacity. Food and Bioprocess Technology 12(3):499-511.

Robles-Sánchez RM, Rojas-Graü, MA, Odriozola-Serrano, I, González-Aguilar, G, Martin-Belloso O (2013). Influence of alginate-based edible coating as carrier of antibrowning agents on bioactive compounds and antioxidant activity in fresh-cut Kent mangoes. LWT - Food Science and Technology 50(1)240-246.

Rodríguez-González VM, Femenia A, González-Laredo RF, Rocha-Guzmán NE, Gallegos-Infante JA, Candelas-Cadillo MG, … Rosselló C (2011). Effects of pasteurization on bioactive polysaccharide acemannan and cell wall polymers from Aloe barbadensis Miller. Carbohydrate Polymers 86(4):1675-1683.

Rößle C, Brunton N, Gormley RT, Wouters R, Butler F (2011). Alginate coating as carrier of oligofructose and inulin and to maintain the quality of fresh-cut apples. Journal of Food Science 76(1):19-29.

Salama HE, Abdel Aziz SM (2021). Development of active edible coating of alginate and aloe vera enriched with frankincense oil for retarding the senescence of green capsicums. LWT-Food Science and Technology 145:111341.

Santos WNLd, da Silva Sauthier MC, dos Santos AMP, de Andrade Santana D, Almeida Azevedo RS, da Cruz Caldas J (2017). Simultaneous determination of 13 phenolic bioactive compounds in guava (Psidium guajava L.) by HPLC-PAD with evaluation using PCA and Neural Network Analysis (NNA). Microchemical Journal 133:583-592.

Sarengaowa Hu, Feng K, Xiu Z, Jiang A, Lao Y (2019). Thyme oil alginate-based edible coatings inhibit growth of pathogenic microorganisms spoiling fresh-cut cantaloupe. Food Bioscience 32:100467.

Sipahi R, Castell-Perez M, Moreira R, Gomes C, Castillo A (2013). Improved multilayered antimicrobial alginate-based edible coating extends the shelf life of fresh-cut watermelon (Citrullus lanatus). LWT - Food Science and Technology 51: 9-15.

Song X, Zuo G, Chen F (2018). Effect of essential oil and surfactant on the physical and antimicrobial properties of corn and wheat starch films. International Journal of Biological Macromolecules 107:1302-1309.

Soukoulis C, Yonekura L, Gan H-H, Behboudi-Jobbehdar S, Parmenter C, Fisk I (2014). Probiotic edible films as a new strategy for developing functional bakery products: The case of pan bread. Food Hydrocolloids 39(100):231-242.

Supapvanich S, Mitrsang P, Srinorkham P, Boonyaritthongchai P, Wongs-Aree C (2016). Effects of fresh Aloe vera gel coating on browning alleviation of fresh cut wax apple (Syzygium samarangenese) fruit cv. Taaptimjaan. Journal of Food Science and Technology 53(6):2844-2850.

Szymanowska U, Złotek U, Karaś M, Baraniak B (2015). Anti-inflammatory and antioxidative activity of anthocyanins from purple basil leaves induced by selected abiotic elicitors. Food Chemistry 172:71-77.

Tabassum N, Khan MA (2020). Modified atmosphere packaging of fresh-cut papaya using alginate based edible coating: Quality evaluation and shelflife study. Scientia Horticulturae 259:108853.

Tridge (2020). Global Production of Guava. Retrieved 07 de mayo 2022 from

Verdini RA, Zorrilla SE, Rubiolo AC (2008). Calcium Uptake during immersion of strawberries in CaCl2 solutions. Journal of Food Science 73(7):533-539.

Wang H, Ding F, Ma L, Zhang Y (2021). Edible films from chitosan-gelatin: Physical properties and food packaging application. Food Bioscience 40:100871.

Wang SY, Gao H (2013). Effect of chitosan-based edible coating on antioxidants, antioxidant enzyme system, and postharvest fruit quality of strawberries (Fragaria x ananassa Duch.). LWT - Food Science and Technology 52(2):71-79.

Yadav A, Kumar N, Upadhyay A, Fawole OA, Mahawar MK, Jalgaonkar K, . . . Mekhemar M (2022). Recent advances in novel packaging technologies for shelf-life extension of guava fruits for retaining health benefits for longer duration. Plants 11(4):547.

Zhang L, Li S, Dong Y, Zhi H, Zong W (2016). Tea polyphenols incorporated into alginate-based edible coating for quality maintenance of Chinese winter jujube under ambient temperature. LWT-Food Science and Technology 70:155-161.



How to Cite

LUNA-ZAPIÉN, E. A., MINJARES-FUENTES, R., SIERRA-CAMPOS, E., MARSZALEK, J. E., BARRAZA-GUERRERO, S. I., & MEZA-VELÁZQUEZ, J. A. (2023). Conservation of commercial quality and bioactive compounds of guava pieces by application of an alginate-acemannan coating. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(1), 13080.



Research Articles
DOI: 10.15835/nbha51113080

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