Сultivation experiment of Origanum vulgare L. in Northern Kazakhstan using nitrogen fertilizer


  • Svetlana KABANOVA A.N. Bukeikhan Kazakh Research Institute of Forestry and Agroforestry, 58 Kirov str., Shchuchinsk (KZ)
  • Pavel SHAKHMATOV A.N. Bukeikhan Kazakh Research Institute of Forestry and Agroforestry, 58 Kirov str., Shchuchinsk (KZ)
  • Valeriy BORTSOV A.N. Bukeikhan Kazakh Research Institute of Forestry and Agroforestry, 58 Kirov str., Shchuchinsk (KZ)
  • Matvey DANCHENKO National Research Tomsk State University, Lenin Street, Tomsk City (KZ)
  • Sabina SCOTT The Ohio State University (US)
  • Andrey KABANOV A.N. Bukeikhan Kazakh Research Institute of Forestry and Agroforestry, 58 Kirov str., Shchuchinsk (KZ)
  • Yana KREKOVA A.N. Bukeikhan Kazakh Research Institute of Forestry and Agroforestry, 58 Kirov str., Shchuchinsk (KZ)
  • Igor KOCHEGAROV A.N. Bukeikhan Kazakh Research Institute of Forestry and Agroforestry, 58 Kirov str., Shchuchinsk (KZ)




cultivation, essential oil, growth, mineral fertilizer, Origanum vulgare L.


The purpose of the research is to determine the effect of ammonium nitrate fertilizer on the growth and yield of Origanum vulgare L. essential oil grown in Northern Kazakhstan. One-factor analysis proved that the introduction of ammonium nitrate positively affected the growth and yield of O. vulgare L., the yield of essential oil and the content of its individual components. Plant height increased by 19.3%, bush size – by 17.4%, yield – by 11.8%. Essential oil output of O. vulgare L. at the experimental site, compared to the control site, increased by 20% and the yield of extraction reached 0.60% and 0.48%, respectively. The largest number of components was related to monoterpenes (52.57% in the experimental section, -50.10% in the control section), the most common were γ-Terpinene, β-Sabinene, Carvacrol and trans-β-Ocimene. Sesquiterpene content in the experimental sample was 37.19%, compared to 38.5250% in the control sample, in which Germacrene D and Caryophyllene were the most common components. Cluster analysis showed that the essential oil composition of O. vulgare L. from Kazakhstan is similar to the composition of the essential oil of plants from Moldova, Poland, Turkey (Anatolia), Latvia and Russia. Germacrene D, Carvacrol and β-Sabinene were the binding components of plants from Kazakhstan and Moldova. The similarity between plants from Kazakhstan and other countries in the same cluster was due to the Germacrene D content (cluster’s average value was 10.1%) and the content of Spathulenol (4.9%).


Ajdarhanova GS, Novak AP, Imasheva BS, Tashev A (2019). Ocenka resursov lekarstvennyh rastenij v lesah Kazahstanskoj chasti Altaya i ih ekologicheskoe sostoyanie [Assessment of medicinal plant resources in the forests of the Kazakh part of Altai and their ecological status]. Vestnik Karagandinskogo universiteta. Seriya Biologiya. Medicina. Geografiya 3(95):72-79.

Akbari B, Baghaei-Yazdi N, Bahmaie M, Abhari FM (2022). The role of plant-derived natural antioxidants in reduction of oxidative stress. Biofactors 48(570):1-23. https://doi.org/10.1002/biof.1831

Al Kamaly O, Alanazi A, Conte R, Imtara H (2023). Phytochemical composition and insight into antibacterial potential of Origanum vulgare essential oil from Saudi Arabia using in vitro and in silico approaches. Processes 11(3):650-655. https://doi.org/10.3390/pr11030650

Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou I (2001). Composition and antimicrobial activity of the essential oils of two Origanum species. Journal of Agricultural and Food Chemistry 49(9):4168-70. https://doi.org/10.1021/jf001494m

Al-Kalaldeh JZ, Abu-Dahab R, Afifi F (2010). Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells. Nutrition Research 30(4):271-279. https://doi.org/10.1016/j.nutres.2010.04.001

Alkan M (2023). Essential oils of Origanum species from Turkey: repellent activity against stored product insect pests. Tarim bilimleri dergisi 29:103-110. https://doi.org/10.15832/ankutbd.898708

Alyakin AA, Efremov AA, Kachin SV, Danilova OO (2010). Frakcionnyj sostav efirnogo masla dushicy obyknovennoj Krasnoyarskogo kraya [Fractional composition of oregano essential oil of the Krasnoyarsk Territory]. Himiya rastitel'nogo syr'ya 1:99-104.

Andrade T, Luiz S, Dutra J, Cruz M, Santos H (2023). Evaluation of the inhibition of strains of Candida albicans by the essential oil of Оriganum vulgare. Brazilian Journal of Development 9:8951-8961. https://doi.org/10.34117/bjdv9n3-008

Asensio СМ, Grosso NR, Juliani Н (2015). Quality characters, chemical composition and biological activities of oregano (Origanum spp.) еessential oils from Central and Southern Argentina. Industrial Сrops and Рroducts 63:203-213. https://doi.org/10.1016/j.indcrop.2014.09.056

Aytac Z, Gülbandılar A, Kürkçüoğlu M (2022). Humic acid improves plant yield, antimicrobial activity and essential oil composition of Oregano (Origanum vulgare L. subsp. hirtum (Link.) Ietswaart). Agronomy 12(9):2086. https://doi.org/10.3390/agronomy12092086

Azizi A, Yan F, Honermeier B (2009). Herbage yield, essential oil content and composition of three oregano (Origanum vulgare L.) populations as affected by soil moisture regimes and nitrogen supply. Industrial crops and products 29:554-561. https://doi.org/10.1016/j.indcrop.2008.11.001

Barreyro R, Ringuelet J, Agricola S (2005). Nitrogen fertilization and yield in Oregan (Origanum хapplii). Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Argentina. https://doi.org/10.7764/rcia.v32i1.305

Başer KНС, Tümen G, Duman H (1997). Essential оil of Origanum acutidens (Hand.-Mazz.) letswaart. Journal of Essential Oil Research 9(1):91-92. https://doi.org/10.1080/10412905.1997.9700721

Bernаth J (1997). Some scientific and practical aspects of production and utilization of oregano in central Europe. Padulosi, S. (Ed.), Oregano, 14. Proceedings of the IPGRI International Workshop. Italy, Rome pp 76-93.

Black C, Haughey S, Chevallier O, Galvin-King P, Elliott C (2016). A comprehensive strategy to detect the fraudulent adulteration of herbs: The oregano approach. Food Chemistry 210:551-557. https://doi.org/10.1016/j.foodchem.2016.05.004

Braga P, DalSasso M, Culici M, Bianchi T, Bordoni L, Marabini L (2006). Anti-inflammatory activity of thymol: inhibitory effect on the release of human neutrophil elastase. Pharmacology 77:130-136. https://doi.org/10.1159/000093790

Bulatova LN (2011). Lekarstvennye dikorastushchie rasteniya predgorij Zailijskogo Alatau [Medicinal wild plants of the foothills of the Trans-Ili Alatau]. Aktual'nye problemy gumanitarnyh i estestvennyh nauk 5:22-26.

Bushkovskaya LM, Pushkina GP, Morozov AI (2011). Regulyatory rosta rastenij v tekhnologiyah zashchity lekarstvennyh kul'tur [Plant growth regulators in medicinal crop protection technologies]. Zashchita i karantin rastenij 9:31-33.

Carrasco A, Perez E, Cutillas AB, Martinez-Gutierrez R, Thomas В, Tudela J (2016). Origanum vulgare and Thymbra capitata essential oils from Spain: determination of aromatic profile and bioactivities. Natural Products Communication 11(1):113-133. https://doi.org/10.1177/1934578X1601100133

Chishti S, Kaloo Z, Sultan Р, Hamida-Tun-Nisa, Nahida-Tun-Nisa (2016). Quantitative analysis of the essential oil of Origanum vulgare (L) growing in the Kashmir valley. International Journal of Applied Research 2(2):552-557.

Chun SS, Vattem DA, Lin Y-T, Shetty K (2005). Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Рrocess Biochemistry 40(2):809-816. https://doi.org/10.1016/j.procbio.2004.02.018

Danin A, Ravid U, Umano K, Shibamoto T (1997). Essential oil composition of Origanum ramonense Danin leaves from Israel. Journal of Essential Oil Research 18(3):250-252.

Danuta К, Kunicka-Styczyńska A (2003). Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry 10(10):813-829. https://doi.org/10.2174/0929867033457719

Deepa TV, Kavitha RD, Gopalakrishnan S (2009). Occurrence of high levels of cadmium, mercury and lead in medicinal plants of India. Pharmacognosy Magazine 5(19):5-8.

Dina E, Vontzalidou A, Cheilari A, Bagatzounis P, Agapidou E, Giannenas I, Grigoriadou K, Aligiannis N (2022). Sustainable use of greek herbs by-products, as an alternative source of biologically active ingredients for innovative products. Frontiers in Nutrition 9:867666. https://doi.org/10.3389/fnut.2022.867666

El-Alam I, Zgheib R, Iriti M, Beyrouthy ME, Verdin A, Fontaine J, Chahine R, Lounès-Hadj А, Makhlouf Н (2019). Origanum syriacum essential oil chemical polymorphism according to soil type. Foods 8(3):90-101. https://doi.org/10.3390/foods8030090

El-Hennawy HM (2018). Medicinal, aromatic and toxic plants in Arab countries. FAO Document Repository 7(2):2116-2119.

Galaktionova EV (2012). Lekarstvennye rasteniya, vhodyashchie v sostav flory Severo-Kazahstanskoj oblasti [Medicinal plants that are part of the flora of the North Kazakhstan region]. Issledovaniya v oblasti estestvennyh nauk 5:21-25.

Gasser U, Klier B, Kuhn AV, Steinhoff B (2009). Current findings on the heavy metal content in herbal drugs. Pharmeuropa 1:37-50.

Giannoulis KD, Kamvoukou C-A, Gougoulias N, Wogiatzi E (2020). Irrigation and nitrogen application affect Greek oregano (Origanum vulgare ssp. hirtum) dry biomass, essential oil yield and composition. Industrial Crops and Products 150(2):112392. https://doi.org/10.1016/j.indcrop.2020.112392

Glavač NK, Djogo S, Ražić S, Kreft S, Veber M (2017). Accumulation of heavy metals from soil in medicinal plants. Arhiv za higijenu rada i toksikologiju 68(3):236-280. https://doi.org/10.1515/aiht-2017-68-2990

Gonceariuc M, Balmuş Z, Sandu T, Romanciuc G, Gonceariuc N (2014) Essential Oil of Origanum vulgare ssp. vulgare L. and Origanum vulgare ssp. hirtum (Link) Ietswaart from Moldova: content and chemical composition. International Journal of Agriculture Innovations And Research 3:659-663.

Gong H, Liu WH, Lv GY, Zhou X (2014). Analysis of essential oils of Origanum vulgare from six production areas of China and Pakistan. Revista Brasileira de Farmacognosia 24(1):25-32. https://doi.org/10.1590 /0102-695X2014241434

Guterres C, Neto A, Alencar L, Loureiro M, Silva F, Ferreira M, Fonseca D, Arruda M, Dias A, Pinheiro L, Everton G (2023). Fungicidal activity of the nanoemulsion (o/w) of Оriganum vulgare essential oil. Research, Society and Development 12(1):e6212139382. https://doi.org/10.33448/rsd-v12i1.39382

Gutiérrez-Grijalva EP, Picos M, Leyva-López N, Criollo-Mendoza MS, Vazquez-Olivo G, Heredia JB (2017). Flavonoids and phenolic acids from oregano: occurrence, biological activity and health benefits. Plants 7(1):2. https://doi.org/10.3390/plants7010002

Hamed ES (2018). Effect of nitrogenous fertilization and spraying with nano-fertilizer on Origanum syriacum L. var. syriacum plants under North Sinai conditions. Journal of Pharmacognosy and Phytochemistry 7(4):2902-2907.

Hazieva FM, Osipov VI, Korotkih IN (2016). Issledovanie vnutrividovoj izmenchivosti efirnogo masla u dushicy obyknovennoj (Origanum vulgare L.) [nvestigation of intraspecific variability of essential oil in oregano (Ogidapim vulgare L.)]. Himiya rastitel'nogo syr'ya 4:97-105.

Hodaj-Çeliku Е, Tsiftsoglou O, Shuka L, Abazi S, Hadjipavlou-Litina D, Lazari D (2017). Antioxidant activity and chemical composition of essential oils of some aromatic and medicinal plants from Albania. Natural Product Communications 12(5):785-790.

Hoffman JM, Lafeuille JL, Ragupathy S, Newmaster S (2021). Spice and herb fraud. Food Fraud 177-218. https://doi.org/10.1016/B978-0-12-817242-1.00005-1

Karamanos AJ, Sotiropoulou DEK (2013). Field studies of nitrogen application on Greek oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart) essential oil during two cultivation seasons. Industrial Crops and Products 46:246-252.

Karaoğlan ES, Özgen U, Kazaz C (2017). Phytochemical studies on Origanum acutidens. International Journal of Medicine and Pharmacy 5(2):23-26. https://doi.org/10.15640/ijmp.v5n2a4

Kazimierczak R., Hallmann Е, Rembiałkowska Е (2014). Effects of organic and conventional production systems on the content of bioactive substances in four species of medicinal plants. Biological Agriculture & Horticulture: An International Journal for Sustainable Production Systems 4.

Kimera F, Sewilam H, Fouad WM, Suloma A (2021). Sustainable production of Origanum syriacum L. using fish effluents improved plant growth, yield, and essential oil composition. Heliyon 7(3):e06423. https://doi.org/10.1016/j.heliyon.2021.e06423

Kocić-Tanackov SD, Dimić GR, Tanackov IJ, Pejin DJ, Mojović LV, Pejin JD (2012). Antifungal activity of Oregano (Origanum vulgare L.) extract on the growth of Fusarium and Penicillium species isolated from food. Hemijska Industrija 66(1):33-41. https://doi.org/10.2298/HEMIND110614073K

Kordali S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E (2008). Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresources Technology 99(18):8788-95. https://doi.org/10.1016/j.biortech.2008.04.048

Kosakowska O, Węglarz Z, Bączek K (2019). Yield and quality of ‘Greek oregano’ (Origanum vulgare L. subsp. hirtum) herb from organic production system in temperate climate. Industrial Crops and Products 141(10):111782. https://doi.org/10.1016/j.indcrop.2019.111782

Kosakowska O, Weglarz Z, Pióro-Jabrucka E, Przybył JL, Krasniewska K, Gniewosz M, Baczek K (2021). Antioxidant and antibacterial activity of essential oils and hydroethanolic extracts of greek oregano (O. vulgare L. subsp. hirtum (Link) Ietswaart) and common oregano (O. vulgare L. subsp. vulgare). Molecules 26(4):988. https://doi.org/10.3390/molecules26040988

Kryeziu T, Haloci E, Loshaj-Shala A, Bagci U, Oral A, Stefkov G, Zimmer A, Basholli-Salihu M (2022). Nanoencapsulation of Origanum vulgare essential oil into liposomes with anticancer potential. Pharmazie 77(6):172-178. https://doi.org/10.1691/ph.2022.1230

Kryvtsova M, Hrytsyna М, Salamon I (2020). Сhemical composition and antimicrobial properties of essential oil from Оriganum vulgare l. in different habitats. Вiotechnologia Acta 13(3):64-72. https://doi.org/10.15407/Вiotech13.03.064

Kryzhko A, Budzhurova U, Ametova E, Novikov I, Soboleva E, Smagliy N, Reshetnik G (2021). Features of the Bacillus thuringiensis 888 strain’s influence on the content of essential oil, antioxidants and flavonoids in Origanum vulgare L. Proceedings of Universities Applied Chemistry and Biotechnology 11(3):430-440. https://doi.org/10.21285/2227-2925-2021-11-3-430-440

Krоl B, Sęczyk Ł, Kołodziej B, PaszkoT (2020). Biomass production, active substance content, and bioaccessibility of Greek oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart) following the application of nitrogen. Industrial Crops and Products 148:112271. https://doi.org/10.1016/j.indcrop.2020.112271

Kulisic T, Radonic A, Katalinic V, Milos M (2004). Analytical, nutritional and clinical methods use of different methods for testing ntioxidative activity of oregano essential oil. Food Chemistry 85(4):633-640. https://doi.org/10.1016/j.foodchem.2003.07.024

Kurkin VA, Kurkina AV, Husainova AI, Ryazanova TK, Sazonova OV (2018). Issledovanie komponentnogo sostava efirnyh masel tim'yana polzuchego i dushicy obyknovennoj, proizrastayushchih v Samarskoj oblasti [Investigation of the component composition of essential oils of creeping thyme and oregano, growing in the Samara region]. Medicinskij vestnik Bashkortostana 2(74):44-47.

Li B, Zheng K, Lu J, Zeng D, Xiang Q, Ma Y. (2022). Antibacterial characteristics of oregano essential oil and its mechanisms against Escherichia coli O157:H7. Journal of Food Measurement and Characterization 16(4):1-10. https://doi.org/10.1007/s11694-022-01393-3

Liu Q, Meng X, Li Y, Zhao CN, Tang GY, Li HB (2017). Аntibacterial and antifungal activities of spices. International Journal of Molecular Sciences 18(6):1283. https://doi.org/10.3390/ijms18061283

Locatelli C, Melucci D, Locatelli M (2014). Toxic metals in herbal medicines. A review. Current Bioactive Compounds 10(3):181-189. https://doi.org/10.2174/1573407210666140716164321

Lombrea A, Antal D, Ardelean F, Avram S, Pavel IZ, Vlaia L, … Danciu C (2020). А recent insight regarding the phytochemistry and bioactivity of Оriganum vulgare l. essential oil. International Journal of Molecular Sciences 21(24):9653. https://doi.org/10.3390/ijms21249653

Ma L, Yao L (2020). Аntiviral effects of plant-derived essential oils and their components: an updated review. Molecules 25(11):2627. https://doi.org/10.3390/molecules25112627

Malik АА. Ahmad J, Abdin MZ (2013). Development of organic cultivation of medicinal plants in the North India. De Gyster Open 59(4):97-98. https://doi.org/10.2478/hepo-2013-0027

Manohar V, Ingram C, Gray J, Talpur NA, Echard BW, Bagchi D, Preuss HG (2001). Antifungal activities of Origanum oil against Candida albicans. Molecular and Cellular Biochemistry 228(1-2):111-118. https://doi.org/10.1023/A:1013311632207

Marchese A, Arciola CR, Coppo E, Barbieri R, Barreca D, Chebaibi S, … Daglia M (2018). The natural plant compound carvacrol as an antimicrobial and anti-biofilm agent: mechanisms, synergies and bio-inspired anti-infective materials. Biofouling 34(6):1-27. https://doi.org/10.1080/08927014.2018.1480756

Martino LD, Feo V, Formisano C, Mignola E, Senatore F (2009). Chemical Сomposition and antimicrobial activity of the essential oils from three chemotypes of Origanum vulgare L. ssp. hirtum (Link) Ietswaart growing wild in Campania (Southern Italy). Molecules 14:2735-2746. https://doi.org/10.3390/molecules14082735

Mirovich VM, Konenkina TA, Fedoseeva GM, Golovnyh NN (2008). Issledovanie kachestvennogo sostava efirnogo masla dushicy obyknovennoj, proizrastayushchej v vostochnoj Sibiri [Investigation of the qualitative composition of the essential oil of oregano, which grows in eastern Siberia]. Himiya rastitel'nogo syr'ya 2:61-64.

Mohamad R, Mussa R, Suslina SN (2021) Prospects for using Origanum Syriacum (L.) as a source of antimicrobial agents. Journal of Advanced Pharmaceutical Technology & Research 12(4):340-344. https://doi.org/10.4103/japtr.japtr_106_21

Moradi М, Hassani А, Ehsani А, Hashemi М, Raeisi М, Naghibi SS (2014). Phytochemical and antibacterial properties of Origanum vulgare ssp. gracile growing wild in Kurdistan Province of Iran. Journal of Food Quality and Hazards Control 1:120-124.

Morshedloo MR, Craker LE, Salami A, Nazeri V, Sang H, Maggi F (2017). Effect of prolonged water stress on essential oil content, compositions and gene expression patterns of mono- and sesquiterpene synthesis in two oregano (Origanum vulgare L.) subspecies. Plant Physiology and Biochemistry 111:119-128. https://doi.org/10.1016/j.plaphy.2016.11.023

Nagar RK, Pandey SB, Vasishth A, Chauhan PS, Ranawat JS (2017). Economics of Aloe barbadensis cultivation suitable for South East region of Rajasthan. The Innovation Journal 6(9):303-305.

Nazar N, Howard C, Slater A, Sgamma T (2022). Сhallenges in medicinal and aromatic plants DNA barcoding—lessons from the Lamiaceae. Plants 11(1):137-163. https://doi.org/10.3390/plants11010137

Ninou E, Cook CM, Papathanasiou F, Aschonitis V, Avdikos I, Tsivelikas AL, Stefanou S, Ralli P, Mylonas I (2021). Nitrogen effects on the essential oil and biomass production of field grown Greek oregano (Origanum vulgare subsp.). Agronomy 11(9):1722. https://doi.org/10.3390/agronomy11091722

Nostro A, Papalia T (2011). Antimicrobial activity of carvacrol: current progress and future prospectives. Recent Patents on Anti-Infective Drug Discovery 7(1):28-35. https://doi.org/10.2174/157489112799829684

Nurmi A, Nurmi T, Mursu T, Hiltunen R, Voutilainen S (2006). Ingestion of oregano extract increases excretion of urinary phenolic metabolites in humans. Journal of Agricultural and Food Chemistry 54(18):6916-6923. https://doi.org/10.1021/jf060879n

Nurzyńska-Wierdak R (2009). Herb yield and chemical composition of common oregano (Origanum vulgare L.) essential oil according to the plant’s developmental stage. Herba Polonica 55(3).

O’Mahony R, AlKhtheeri H, Weerasekera D, Fernando N (2006). Bactericidal and anti-adhesive properties of culinary and medicinal plants against Helicobacter pylori. World Journal of Gastroenterology 11(47):7499-7507.

Özer Z, Carikci S, Yilmaz H, Kilic T, Dirmenci T, Gören A. (2020). Determination of secondary metabolites of Origanum vulgare subsp. hirtum and O. vulgare subsp. vulgare by LC-MS/MS. Journal of Chemical Metrology 14:1-10. https://doi.org/10.25135/jcm.

Ozgüven M, Ayanoglu F, Ozel A (2006). Effects of nitrogen rates and cutting times on the essential oil yield and components of Origanum syriacum L. var. bevanii. Journal of Agronomy 5(1):101-105. https://doi.org/10.3923/ja.2006.101.105

Özkan O, Güney K, Gür M, Pattabanoğlu E, Babat E, Khalifa M (2017). Essential oil of oregano and savory; chemical composition and antimicrobial activity. Indian Journal of Pharmaceutical Education and Research 51(3):205-208. https://doi.org/10.5530/ijper.51.3s.13

Paknejad P, Yoonessi A, Hatami J, Semsarha F (2020). The effectiveness of Оriganum vulgare (Оriganum vulgare l. ssp. viridis) essential oil inhalation on short term memory. Conference: The Second Iranian Neuropsychiatry Congress. Tehran, Iran.

Pande С, Tewari G, Singh S, Singh С (2011). Chemical markers in Origanum vulgare L. from Kumaon Himalayas: a chemosystematic study. Natural Product Research 26(2):140-145. https://doi.org/10.1080/14786419.2010.53515

Patel КМ, Rabari K (2022). Effect of phosphorus and biofertilizers on yield and economics of black gram. International Journal of Agricultural Sciences 14(4):11234-11236.

Pereira JB, Dantas KG (2016). Evaluation of inorganic elements in cat’s claw teas using ICP OES and GF AAS. Food Chemistry 196:331-338.

Radušienė J, Stankevičienė D, Venskutonis R (2005). Morphological and chemical variation of Origanum vulgare L. from Lithuania. Acta Horticulturae 675(675):197-203. https://doi.org/10.17660/ActaHortic.2005.675.29

Raina AP, Negi KS (2014). Chemical diversity among different accessions of Origanum vulgare L. ssp. vulgare collected from Central Himalayan region of Uttarakhand, India. Journal of Essential Oil Research 26(6):1-7. https://doi.org/10.1080/10412905.2014.948969

Rekha B, Veeranna Hk, Shashikala K, Gajendra K (2018). Organic fertilizer management in cultivation of medicinal and aromatic crops: a review. Journal of Pharmacognosy and Phytochemistry 3:126-129.

Russo M, Galletti G, Bocchini P, Carnacini A (1998). Essential oil composition of wild populations of Italian oregano spice (Origanum vulgare spp. Hirtum (Link) Ietswaart): a preliminary evaluation of their use in chemotaxonomy by cluster analysis. 1. Influorescences. Journal of Agricultural and Food Chemistry 46:3741-3746.

Şahin F, Güllüce M, Daferera D, Sökmen A, Sökmen M, Polissiou M, Agar G, Özer H (2004). Biological activities of the essential oils and methanol extract of Origanum vulgare in the Eastern Anatolia region of Turkey. Food Control 15(7):549-557. https://doi.org/10.1016/j.foodcont.2003.08.009

Said-Al- Ahl HAH, Omer E, Naguib NY (2009). Effect of water stress and nitrogen fertilizer on herb and essential oil of oregano. International Agrophysics 23(3):269-275.

Sarrou Е, Tsivelika N, Chatzopoulou P, MavromatisА (2016). Evaluation of agronomical and qualitative characteristics of Greek Oregano (Origanum vulgare ssp.). 6 th International Symposium Breeding Research on Medicinal and Aromatic Plants, Breedmap 6, Quedl inburg, Germany, June 19-23:139.

Shamseddine L, Chidiac JJ (2020). Composition’s effect of Origanum syriacum essential oils in the antimicrobial activities for the treatment of denture stomatitis. Odontology 109(2):327-335. https://doi.org/10.1007/s10266-020-00547-3

Sharifi-Rad M, Yılmaz YB, Antika G, Salehi B, Tumer TB, Venil CK. (2021). Phytochemical сonstituents, biological activities, and health-promoting effects of the genus Origanum. Phytotherapy Research 35(1):95-121. https://doi.org/10.1002/ptr.6785

Simirgiotis MJ, Burton D, Parra F, López J, Muñoz P, Escobar H, Parra C (2020). Antioxidant and antibacterial capacities of Origanum vulgare L. essential oil from the arid Andean region of Chile and its chemical characterization by GC-MS. Metabolites 10(10):414-426. https://doi.org/10.3390/metabo10100414

Singletary KW (2010). Oregano: overview of the literature on health benefits. Nutrition Today 45(3):129-138. https://doi.org/10.1097/NT.0b013e3181dec789

Soltani S, Shakeri A, Iranshahi M, Boozari M (2021). A review of the phytochemistry and antimicrobial properties of Оriganum vulgare l. and subspecies. Iranian Journal of Pharmaceutical Research (IJPR) 20(2):268-285. https://doi.org/10.22037/ijpr.2020.113874.14539

Soylak M, Tuzen M, Narin I, Sari H (2004). Comparison of microwave, dry and wet digestion procedures for the determination of trace metal contents in spice samples produced in Turkey. Journal of Food and Drug Analysis 12(3):254-262. https://doi.org/10.38212/2224-6614.2634

Stešević D, Jaćimović Ž, Šatović Z, Šapčanin A, Jančan G, Kosović M, Damjanović-Vratnica B (2018). Chemical characterization of wild growing Origanum vulgare populations in Montenegro. Natural Product Communications, 13(10):1357-1362. https://doi.org/10.1177/1934578X1801301031

Stoilova I, Bail S, Buchbauer G, Krastanov A, Stoyanova A, Schmidt E, Jirovetz L (2008). Chemical composition, olfactory evaluation and antioxidant effects of an essential oil of Origanum vulgare L. from Bosnia. Natural Рroduct Сommunications 3(7):1043-1046. https://doi.org/10.1177/1934578X0800300702

Susurluk H. (2023). Potential use of essential oils from Origanum vulgare and Syzygium aromaticum to control Tetranychus urticae Koch (Acari: Tetranychidae) on two host plant species. PeerJ 11:e14475. https://doi.org/10.7717/peerj.14475

Szabо K, Sаrosi Sz, Cserhаti B, Ferenczy A (2010). Can glandular hair density be a breeding marker for Origanum vulgare subsp. hirtum with high essential oil content? Natural Product Communications 5(9):1437-1440. https://doi.org/10.1177/1934578X1000500918

Tariyal YS, Ansari P (2021). Prasad organic cultivation of medicinal and aromatic plants: a review. Journal of Stress Physiology & Biochemistry 17(1):129-133.

Teixeira В, Marques А, Ramos С, Serrano С, Matos О, Neng NR, Nogueira JMF, Saraiva JА, Nunes ML (2013). Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. Journal of the Science of Food And Agriculture 93 (11):2707-2714. https://doi.org/10.1002/jsfa.6089

Tokalıoğlu Ş (2012). Determination of trace elements in commonly consumed medicinal herbs by ICP-MS and multivariate analysis. Food Chemistry 134(4):2504-2512. https://doi.org/10.1016/j.foodchem.2012.04.093

Toncer О, Karaman S, Diraz Е (2010) An annual variation in essential oil composition of Origanum syriacum from Southeast Anatolia of Turkey. Journal of Medicinal Plants Research 4(11):1059-1064.

Ulupinar S, Maral H, Özbay S, Gençoğlu C, Türk Baydir A, Şebin E, … Buzdağlı Y (2022). Еffect of Оriganum dubium, Оriganum vulgare subsp hirtum, and Lavandula angustifolia essential oils on immune cells in athletes. Israel Journal of Plant Sciences 1(8):113-120. https://doi.org/10.1163/22238980-bja10067

Vazirian М, Mohammadi М, Farzaei MH, Amin G, Amanzadeh Y (2015). Chemical composition and antioxidant activity of Origanum vulgare subsp. vulgare essential oil from Iran. Research Journal of Pharmacognosy 2:41-46.

Veres К, Varga Е, Schelz Z, Molnár J, Bernáth J, Máthé I (2007). Сhemical composition and antimicrobial activities of essential oils of four lines of Origanum vulgare subsp. hirtum (link) ietswaart grown in Hungary. Natural Рroduct Сommunications 2(11):1155-1158. https://doi.org/10.1177/1934578X0700201122

Virga G, Sabatino L, Licata M, Tuttolomondo T, Leto C, La Bella S (2020). Effects of irrigation with different sources of water on growth, yield and essential oil compounds in oregano. Plants 9(11):1618. https://doi.org/10.3390/plants9111618

Vityaz SN, Rakina MS, Pozdnyakova OG, Kazakova MA (2019). Vliyanie razlichnyh priemov vozdelyvaniya lekarstvennyh trav na elementy urozhajnosti i kachestvo lekarstvennogo syr'ya [The influence of various methods of cultivation of medicinal herbs on the elements of yield and quality of medicinal raw materials]. Dostizhenie nauki i tekhniki APK 12:60-64.

Voidarou C, Rozos G, Alexopoulos А, Plessas S, Mantzourani I, Stavropoulou E, Tzora A, Bezirtzoglou E (2021). In vitro screening potential antibacterial properties of the Greek oregano honey against clinical isolates of Helicobacter pylori. Foods 10(7):1568. https://doi.org/10.3390/foods10071568

Węglarz Z, Kosakowska O, Przybył JL, Pióro-Jabrucka E, Bączek K (2020). The quality of Greek oregano (О. vulgare L. subsp. hirtum (Link) Ietswaart) and common oregano (O. vulgare L. subsp. vulgare) cultivated in the temperate climate of Central Europe. Foods 9(11):1671. https://doi.org/10.3390 /foods9111671

Yoncheva K, Benbassat N, Zaharieva MM, Dimitrova L, Kroumov A, Spassova I, Kovacheva D, Najdenski HM (2021). Improvement of the antimicrobial activity of oregano oil by encapsulation in chitosan–alginate nanoparticles. Molecules 26(22):7017. https://doi.org/10.3390/molecules26227017

Yue Y, Sun J, Song Y, Raka R, Xiang J, Wu H, Xiao J, Jin J, Hui X (2023). Antibacterial activity of oregano essential oils against Streptococcus mutans in vitro and analysis of active components. BMC Complementary Medicine and Therapies 23(1):61-74. https://doi.org/10.1186/s12906-023-03890-4

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KABANOVA, S., SHAKHMATOV, P., BORTSOV, V., DANCHENKO, M., SCOTT, S., KABANOV, A., KREKOVA, Y., & KOCHEGAROV, I. (2023). Сultivation experiment of Origanum vulgare L. in Northern Kazakhstan using nitrogen fertilizer . Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(1), 13077. https://doi.org/10.15835/nbha51113077



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DOI: 10.15835/nbha51113077