Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors

Authors

  • Asrar M. ALSWAT University of Jeddah, College of Science, Department of Biology, Jeddah 21493 (SA)
  • Rewaa S. JALAL University of Jeddah, College of Science, Department of Biology, Jeddah 21493 (SA)
  • Ashwag Y. SHAMI Princess Nourah bint Abdulrahman University, College of Sciences, Department of Biology, P.O. Box 84428, Riyadh 11671 (SA)
  • Mazen A. MAJEED University of Jeddah, College of Science, Department of Biology, Jeddah 21493 (SA)
  • Zohoor S. ALSAEDI University of Jeddah, College of Science, Department of Biology, Jeddah 21493 (SA)
  • Lina BAZ King Abdulaziz University, Faculty of Science, Department of Biochemistry, Jeddah 21589 (SA)
  • Mohammed Y. REFAI University of Jeddah, College of Science, Department of Biochemistry, Jeddah 21493 (SA)
  • Mohammed N. BAESHEN University of Jeddah, College of Science, Department of Biology, Jeddah 21493 (SA)
  • Noor M. BATAWEEL King Fahad Medical Research Center, P.O. Box 80216, King Abdulaziz University, Jeddah, 21589 (SA)
  • Ahmed AL-HEJIN King Abdulaziz University, Faculty of Science, Department of Biological Sciences, Jeddah (SA)
  • Ruba A. ASHY University of Jeddah, College of Science, Department of Biology, Jeddah 21493 (SA)

DOI:

https://doi.org/10.15835/nbha51113053

Keywords:

metagenomics, microbiome, PGPR, rhizosphere, Senna italica plant

Abstract

Natural microbial communities associated with desert plants are found in soils that face nutrient deficiencies and extreme environments, including salinity and drought. In this study, 16S rRNA metagenomic sequencing was used to screen and identify bacterial assemblies associated with the desert plant Senna italica, obtained from diverse soil samples located in the Asfan region, northeast of Jeddah, Saudi Arabia. Several studies found Senna italica as a valuable medicinal plant for treating different diseases; however, a few studies were done on its association with bacterial communities under drought conditions. This study aimed to identify bacterial communities present in the drought soil environment of the Senna italica plants. To approach our goals, we applied metagenomic techniques, discovering a new bacterial strain beneficial for biotechnological applications. Our results showed that the analysis of the 16S rRNA sequences at the taxonomic phylum level detected 15 phyla of bacterial populations in the soil samples. The most prevalent was kept for further research. Our findings demonstrated that rhizospheric bacteria may be used as indicators of plant growth rate and survival ability in hostile environments. Studying the soil microbiome's taxonomic, phylogenetic, and functional diversity will facilitate identifying new candidates for biological agents that can be used to improve agricultural and industrial processes.

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Published

2023-02-20

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ALSWAT, A. M., JALAL, R. S., SHAMI, A. Y., MAJEED , M. A., ALSAEDI, Z. S., BAZ, L., REFAI, M. Y., BAESHEN, M. N., BATAWEEL, N. M., AL-HEJIN, A., & ASHY , R. A. (2023). Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(1), 13053. https://doi.org/10.15835/nbha51113053

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Vol. 51 No. 1 (2023)

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Research Articles
CITATION
DOI: 10.15835/nbha51113053

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Copyright (c) 2023 Asrar M. ALSWAT, Rewaa S. JALAL, Ashwag Y. SHAMI, Mazen A. MAJEED , Zohoor S. ALSAEDI, Lina BAZ, Mohammed Y. REFAI, Mohammed N. BAESHEN, Noor M. BATAWEEL, Ahmed AL-HEJIN, Ruba A. ASHY

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