Leaf disease caused by Epicoccum thailandicum on Amomum villosum in China
DOI:
https://doi.org/10.15835/nbha51113021Keywords:
Amomum villosum, Epicoccum thailandicum, leaf disease, pathogenic fungi, phylogenetic analysesAbstract
Amomum villosum is widely planted as a cash crop and medicinal plant. Recent reports of leaf diseases that result in yield reduction of Amomum villosum fruits raise concerns, which may play an important role in yield losses. Understanding the aetiology of current and new diseases of Amomum villosum is important to address production issues and to formulate effective disease control measures. An organizational separation method was used to isolate phytopathogen from leaves. Ten fungi were isolated from the diseased leaves. Among the selected microorganisms, fungi AP5 showed typical pathogenic symptoms and fulfilling Koch’s requirements. The rDNA internal transcribed spacer region (ITS) with primers ITS1/ITS4, 28S ribosomal RNA gene (LSU) with primers NL1/NL4, RNA polymerase II second largest subunit (RPB II) with primers fRPB2-5f/fRPB2-7cR, and beta-tubulin (tub2) with primers BT2a/BT2b of pathogenic strain were amplified and sequenced. AP5 was identified as Epicoccum thailandicum based on morphological and phylogenetic analyses. This is the first report that Epicoccum thailandicum is the pathogenic fungi infecting Amomum villosum leaves.
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Azuddin NF, Mohd MH, Rosely NFN, Mansor A, Zakaria L (2021). Molecular phylogeny of endophytic fungi from rattan (Calamus castaneus griff.) spines and their antagonistic activities against plant pathogenic fungi. Journal of Fungi 7(4):301. https://doi.org/10.3390/jof704030
Belisário R, Robertson AE, Vaillancourt LJ (2022). Maize anthracnose stalk rot in the genomic era. Plant Diseases 106(9):2281-2298. https://doi.org/10.1094/PDIS-10-21-2147-FE
Boekhout T, Fell JW, O'Donnell K (1995). Molecular systematics of some yeast-like anamorphs belonging to the ustilaginales and tilletiales. Studies in Mycology 38:175-183.
Callan BE, Carris LM (2004). Fungi on living plant substrata, including fruits. Biodiversity of Fungi 105-126. https://doi.org/10.1016/B978-012509551-8/50010-6
Glass NL, Donaldson GC (1995). Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61:1323-1330.
https://doi.org/10.1128/AEM.61.4.1323-1330
Han VC, Yu NH, Yoon H, Son YK, Lee BH, Kim JC (2021). First report of Epicoccum tobaicum associated with leaf sport on flowering cherry in south Korea. Plant Disease 105(7):2734. https://doi.org/10.1094/PDIS-12-20-2711-PDN
Hou LW, Groenewald JZ, Pfenning LH, Yarden O, Crous PW, Cai L (2020). The phoma-like dilemma. Studies in Mycology 96:309-396. https://doi.org/10.1016/j.simyco.2020.05.001
Kirk PM, Cannon PF, Minter DW, Stalpers JA (2008). Dictionary of the Fungi. CAB International, Wallingford.
Thambugala KM, Wanasinghe DN, Phillips AJL, Camporesi E, Bulgakov TS, Phukhamsakda C, ... Hyde KD (2017). Mycosphere notes 1–50: grass (Poaceae) inhabiting Dothideomycetes. Mycosphere 8(4):697-796. https://doi.org/10.5943/mycosphere/8/4/13
Koch R (1878). Untersuchungen Über die Aetiologie der Wundinfectionskrankheiten. FCW Vogel, Leipzig.
Li G, Chen AJ, Chen XY, Li XL, Gao WW (2010). First report of Amomum villosum (cardamom) leaf lesion caused by Pyricularia costina in China. New Disease Reports 22:2. https://doi.org/10.5197/j.2044-0588.2010.022.002
Liu YJ, Whelen S, Hall BD (1999). Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 6:1799-1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
Lu JY (2001). Mycology of plant pathogens. China Agriculture Press, Beijing.
Ridzuan R, Rafii M, Ismail S, Mohammad YM, Miah G, Usman M (2018). Breeding for anthracnose disease resistance in chili: progress and prospects. International Journal of Molecular Sciences 19(10):3122. https://doi.org/10.3390/ijms19103122
Sang WJ, Song BA, Lian QX, Hu DY, Huang RM, Li XX (2006). Identification of Amomum villosum anthracnose on and fungicide toxicity determination. Agrochemicals 8:22. https://doi.org/10.16820/j.cnki.1006-0413.2006.08.022
Tian Y, Zhang YY, Qiu CD, Liu ZY (2021). First report of leaf spot of Weigela florida caused by Epicoccum layuense in China. Plant Diseases 105(8):2243. https://doi.org/10.1094/PDIS-07-20-1498-PDN
White TJ, Bruns T, Lee S, Taylor J (1990). PCR - Protocols and Applications - A Laboratory Manual. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ(Eds). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Academic Press, San Diego, USA, pp315-322.
Yin QX, Jiang SL, Li DX, Huang HL, Wang Y, Wan DL (2022). First report of Epicoccum nigrum causing brown leaf spot in tea in Guizhou Province, China. Plant Disease 106(1):321. https://doi.org/10.1094/PDIS-04-21-0815-PDN
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Copyright (c) 2022 Shanqi WANG, Zixiang LI, Wenmin FENG, Yufan CHEN, Ying ZHANG
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