In vitro germination and regeneration of Senna artemisioides, a valuable leguminous ornamental shrub


  • Konstantinos BERTSOUKLIS Agricultural University of Athens, School of Plant Sciences, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, Iera Odos 75, 11855 Athens (GR)
  • Kostandin NAKSI Agricultural University of Athens, School of Plant Sciences, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, Iera Odos 75, 11855 Athens (GR)
  • Paraskevi-Evangelia ARETAKI Agricultural University of Athens, School of Plant Sciences, Department of Crop Science, Laboratory of Floriculture and Landscape Architecture, Iera Odos 75, 11855 Athens (GR)



climatisation, in vitro rooting, micropropagation, new ornamental, tissue culture


Τhis research was carried out at the prospect of introducing new ornamental species in the floriculture industry concerning the use of valuable plants. A leguminous species native to Australia was the object of the present study.  Seeds of Senna artemisioides established in a private botanical garden were chosen as the starting material for both germination and in vitro studies. Hot water treatment at 90 °C, for 10 min was applied to interrupt the seed-coat dormancy. Incubation was conducted in vitro in Petri dishes, at seven different temperatures ranging from of 5.0 to 35.0 °C, involving three separate treatments of incubation at 5.0 °C, for 0, 30 and 60 days. The seeds germinated at 25 °C (56.0%). Node explants derived from produced seedlings were cultured in vitro onto Murashige and Skoog media (MS), hormone free (Hf) or containing 6-Benzyladenine (BA). The establishment of initial cultures was successful being higher on MS supplemented with BA at the range 0.1-1.0 mg L-1 media. The multiplication phase followed on MS media containing the same range of a total of four cytokinins widely used in in vitro experimentation, i.e BA, Kinetin (KIN), N6-Isopentenyladenine (2IP) and Zeatin (ZEA). 1-Naphthaleneacetic acid (NAA) was tested in conjunction with the higher concentration of the four cytokinins. MS media supplemented with BA at 0.5 mg L-1 led to a satisfactory proliferation rate; 2.4 shoots/explant were formed. Rooting phase had moderate efficiency, being higher for media with 4.0 mg L-1 IBA. Acclimatisation was successful for all rooted plantlets, promising an effective method for exploitation of this new ornamental species of high value. 


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How to Cite

BERTSOUKLIS, K., NAKSI, K., & ARETAKI, P.-E. (2023). In vitro germination and regeneration of Senna artemisioides, a valuable leguminous ornamental shrub. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 51(1), 12992.



Research Articles
DOI: 10.15835/nbha51112992