Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet  Light Emitting Diodes (LEDs) combined with cytokinins  under tissue culture conditions

Lina M. ABU-ZIADA; Samia A. HAROUN; Rasha M.E. GAMEL; Mohamed A. ABBAS; Mahmoud R. SOFY; Heba Y. EL-BANNA

doi:10.15835/nbha50312879

Authors

Lina M. ABU-ZIADA Mansoura University, Faculty of Science, Botany Department, 35516 Mansoura (EG)
Samia A. HAROUN Mansoura University, Faculty of Science, Botany Department, 35516 Mansoura (EG)
Rasha M.E. GAMEL Mansoura University, Faculty of Science, Botany Department, 35516 Mansoura (EG)
Mohamed A. ABBAS Mansoura University, Faculty of Science, Botany Department, 35516 Mansoura (EG)
Mahmoud R. SOFY Al Azhar University, Faculty of Science, Botany and Microbiology Department, 11884 Nasr City, Cairo (EG)
Heba Y. EL-BANNA Mansoura University, Faculty of Agriculture, Vegetable and Floriculture Department, 35516 Mansoura (EG)

DOI:

https://doi.org/10.15835/nbha50312879

Keywords:

benzyl aminopurine, light-emitting diode systems (LEDs), Moringa oleifera, secondary metabolites, thidiazuron, tissue culture

Abstract

The present study was carried out between 2021 and 2022 at the tissue culture lab and experimental station of the vegetable and floriculture department, faculty of agriculture, Mansoura University. Using mature seeds, Moringa oleifera is propagated on a large scale and in rapid succession by in vitro culture. Two types of cytokinin: 6-benzylaminopurin (BAP), and thidiauron (TDZ) singly at three different concentrations (0, 1, or 2 mg/L), were used, as well as light-emitting diode systems (LEDs) (white as control and violet; the combination of red and blue; 1:1). After incubation for 30 days, the obtained results showed that the survival percentage increased by the treatments with the maximum value (85%) by MS medium supplemented with 2 mg/L TDZ, under violet LEDs illumination. However, the addition of thidiazuron (TDZ) to the medium did not propagate shoot, and this treatment recorded 100% callus formation other than BAP. The maximum number of axillary shoots per explant and the number of leaves recorded in the MS medium contained 2 mg/L BAP under violet LEDs. Compared to the control values, all the used treatments generally caused surprisingly stimulating the determined antioxidant enzymes and compounds (peroxidase, polyphenol oxidase &catalase and phenols, flavonoids, anthocyanin& ascorbic acid). Concerning the phytohormone content of the hormone-free medium (control), IAA, kinetin, and zeatin increased. Meanwhile, GA₃ and ABA decreased with the used treatments.

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