Foliar spray of Si and Ti nanoparticles affected enzymatic antioxidants in rapeseed (Brassica napus L.)


  • Velibor SPALEVIC University of Montenegro, Biotechnical Faculty, 81000 Podgorica; University of Montenegro, Geography, Faculty of Philosophy, 81400 Niksic (ME)
  • Branislav DUDIC Comenius University Bratislava, Faculty of Management, 82005 Bratislava; University Business Academy, Faculty of Economics and Engineering Management, 21000 Novi Sad (SK)
  • Sezai ERCIŞLI Ataturk University, Faculty of Agriculture, Department of Horticulture, 25240, Erzurum (TR)
  • Mohsen JANMOHAMMADI University of Maragheh, Faculty of Agriculture, Department of Plant Production and Genetic, P.O. Box 55181-83111, Maragheh (IR)
  • Naser SABAGHNIA University of Maragheh, Faculty of Agriculture, Department of Plant Production and Genetic, P.O. Box 55181-83111, Maragheh (IR)



ascorbate peroxidase, catalase, hydrogen peroxide, membrane integrity, ROS scavenging


Some beneficial nanoparticles such as silicon and titanium are encouraging in foliar feeding due to their large surface area and biocompatible properties. In current experiment, seedling of rapeseed varieties (‘Hydromel’, ‘Nathalie’, ‘Alonso’) was sprayed with titanium dioxide and silicon dioxide nanoparticles (Ti and Si-NPs) and then responses of some enzymatic antioxidant and plasma membrane integrity were evaluated. Foliar application of Si-NPs significantly increased the activity of ascorbate peroxidase compared to the control, in all three varieties. Spray of both Ti and Si-NPs increased guaiacol peroxidase activity in ‘Nathalie’ and ‘Alonso’ varieties while in ‘Hydromel’ only Si-NPs spray had positive effects on activity this antioxidant. Behaviors of superoxide dismutase (SOD) isozymes under different foliar treatments and in varieties were different, but the highest activity of total SOD isozyme in ‘Hydromel’ and ‘Alonso’ varieties was obtained by foliar application of Si-NPs and Ti-NPs, respectively. The lowest amount of hydrogen peroxide was obtained by foliar application of Ti-NPs. Examination of malondialdehyde (MDA) also showed that the most vulnerable membrane belonged to ‘Hydromel’ variety and foliar application of Si-NPs and Ti-NPs could improve membrane integrity. These results suggest that foliar application of both Si-NPs and Ti-NPs improves the scavenging capacity of reactive oxygen species (ROS), although the response of the enzymatic antioxidants was largely influenced by the variety.


Aebi H (1984). Catalase in vitro. Methods in Enzymology 105:121-126.

Ahmed R, Yusoff Abd Samad M, Uddin M, Quddus M, Hossain MA (2021). Recent trends in the foliar spraying of zinc nutrient and zinc oxide nanoparticles in tomato production. Agronomy 11(10):2074.

Asgari F, Majd A, Jonoubi P, Najafi F (2018). Effects of silicon nanoparticles on molecular, chemical, structural and ultrastructural characteristics of oat (Avena sativa L.). Plant Physiology and Biochemistry 127:152-160.

Basahi M (2021). Seed germination with titanium dioxide nanoparticles enhances water supply, reserve mobilization, oxidative stress and antioxidant enzyme activities in pea. Saudi Journal of Biological Sciences 28(11):6500-6507.

Beauchamp C, Fridovich I (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44(1):276-287.

Chaudhary IJ, Singh V (2020). Titanium dioxide nanoparticles and its impact on growth, biomass and yield of agricultural crops under environmental stress: A review. Research Journal of Nanoscience and Nanotechnology 10:1-8.

Fattahi M, Janmohammadi M, Dashti S, Nouraein M, Sabaghnia N (2018). Effects of nitrogen and micronutrients on the growth of safflower under limited water conditions in a high-elevation region. Biologija 64(3):235-248.

Fernandez V, Brown PH (2013). From plant surface to plant metabolism: the uncertain fate of foliar-applied nutrients. Frontiers in Plant Science 4:289.

Heath RL, Packer L (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. In: Archives of Biochemistry and Biophysics 125:189-198.

Ito F, Sono Y, Ito T (2019). Measurement and clinical significance of lipid peroxidation as a biomarker of oxidative stress: oxidative stress in diabetes, atherosclerosis, and chronic inflammation. Antioxidants 8(3):72.

Janmohammadi M (2012). Alleviation the adverse effect of cadmium on seedling growth of greater burdock (Aractium lappal L.) through pre-sowing treatments. Agriculture and Forestry 56(1-4):55.

Janmohammadi M, Amanzadeh T, Sabaghnia N, Dashti S (2016). Impact of foliar application of nano micronutrient fertilizers and titanium dioxide nanoparticles on the growth and yield components of barley under supplemental irrigation. Acta Agriculturae Slovenica 107(2):265-276.

Janmohammadi M, Mohamadi N, Shekari F, Abbasi A, Esmailpour M (2017). The effects of silicon and titanium on safflower (Carthamus tinctorius L.) growth under moisture deficit condition. Acta Agriculturae Slovenica 109(2):443-455.

Kolarić L, Popović V, Živanović L, Ljubičić N, Stevanović P, Šarčević Todosijević Lj, … Ikanović J (2021). Buckwheat yield traits response as influenced by row spacing, nitrogen, phosphorus, and potassium management. Agronomy 11: 2371.

Lakić Ž, Predić T, Đurđić I, Popović V (2020). Recultivation of degraded soil due to mining activity without adding organic layers of soil using аlfalfa and mixtures of grass legumes. Agriculture and Forestry 66(4):223-237.

Leonowicz G, Trzebuniak KF, Zimak-Piekarczyk P, Ślesak I, Mysliwa-Kurdziel B (2018). The activity of superoxide dismutases (SODs) at the early stages of wheat deetiolation. Plos One 13(3):e0194678.

Martins D, English AM (2014). Catalase activity is stimulated by H2O2 in rich culture medium and is required for H2O2 resistance and adaptation in yeast. Redox Biology 2:308-313.

Navari-Izzo F, Quartacci MF, Pinzino C, Dalla vecchia F, Sgherri CLM (1998). Thylakoid-bound and stromal antioxidative enzymes in wheat treated with excess copper. Physiologia Plantarum 104:630-638.

Peel MC, Finlayson BL, Mcmahon TA (2007). Updated world map of the Köppen-Geiger climate classification. In: Hydrology and Earth System Sciences Discussions 4(2):439-473.

Popović D, Vitomir J, Jokić M, Arnautović I, Vrhovac D, Barović N, … Popović S (2020c). Implementation of internal audit in companies intending to operate on the principles of green economy in the Republic of Serbia. Agriculture and Forestry 66(2):93-98.

Popović D, Vitomir J, Tomaš-Miskin S, Davidov T, Popović S, Jovanović M, … Jovanović S (2021). Implementation of internal control with reference to the application of "IT" in companies operating on the principles of the green economy. Agriculture and Forestry 67(2):257-266.

Popović V, Ljubičić N, Kostić M, Radulović M, Blagojević D, Ugrenović V, Popović D, Ivošević B (2020). Genotype × environment interaction for wheat yield traits suitable for selection in different seed priming conditions. Plants 9(12):1804.

Popović V, Vučković S, Jovović Z, Ljubičić N, Kostić M, Rakaščan N, ... Ikanović J (2020a). Genotype by year interaction effects on soybean morpho-productive traits and biogas production. Genetika 52(3):1055-1073.

Prasad VB, Govindaraj M, Djanaguiraman M, Djalovic I, Shailani A, Rawat N, … Prasad PV (2021). Drought and high temperature stress in sorghum: Physiological, genetic, and molecular insights and breeding approaches. International Journal of Molecular Sciences 22(18):9826.

Raboanatahiry N, Li H, Yu L, Li M (2021). Rapeseed (Brassica napus): Processing, utilization, and genetic improvement. Agronomy 11(9):1776.

Rajput VD, Minkina T, Feizi M, Kumari A, Khan M, Mandzhieva S, … Hullebusch EDV (2021). Effects of silicon and silicon-based nanoparticles on rhizosphere microbiome, plant stress and growth. Biology 10(8):791.

Rizwan M, Ali S, Rehman MZ, Malik S, Adrees M, Qayyum MF, … Ahmad P (2019). Effect of foliar applications of silicon and titanium dioxide nanoparticles on growth, oxidative stress, and cadmium accumulation by rice (Oryza sativa). Acta Physiologiae Plantarum 41(3):1-12.

Saa S, Rio OD, Castro S, Brown PH (2015). Foliar application of microbial and plant based biostimulants increases growth and potassium uptake in almond (Prunus dulcis [Mill.] DA Webb). Frontiers in Plant Science 6:87.

Siddiqui MH, Al‐Whaibi MH, Faisal M, Al Sahli AA (2014). Nano‐silicon dioxide mitigates the adverse effects of salt stress on Cucurbita pepo L.). Environmental Toxicology and Chemistry 33:2429-2437.

Steel RGD, Torrie GH (1980). Principles and Procedures of Statistics. 2nd Ed. Singapore: McGraw Hill Book Co. Inc.

Strout G, Russell SD, Pulsifer DP, Erten S, Lakhtakia A, Lee DW (2013). Silica nanoparticles aid in structural leaf coloration in the Malaysian tropical rainforest understorey herb Mapania caudata. Annals of Botany 112(6):1141-1148.

Wang Y, Branicky R, Noë A, Hekimi S (2018). Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling. Journal of Cell Biology 217(6):1915-1928.



How to Cite

SPALEVIC, V., DUDIC, B., ERCIŞLI, S., JANMOHAMMADI, M., & SABAGHNIA, N. (2022). Foliar spray of Si and Ti nanoparticles affected enzymatic antioxidants in rapeseed (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(4), 12859.



Research Articles
DOI: 10.15835/nbha50412859

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