Efficient micropropagation protocol of three cultivars of highbush blueberry (Vaccinium corymbosum L.)


  • Monika FIGIEL-KROCZYŃSKA West Pomeranian University of Technology Szczecin, Department of Horticulture, Słowackiego 17 Street, 71-434 Szczecin (PL)
  • Marcelina KRUPA-MAŁKIEWICZ West Pomeranian University of Technology Szczecin, Department of Plant Genetics, Breeding and Biotechnology, Szczecin 71-434 (PL)
  • Ireneusz OCHMIAN West Pomeranian University of Technology Szczecin, Department of Horticulture, Słowackiego 17 Street, 71-434 Szczecin (PL)




acclimatization, highbush blueberry, in vitro, plant growth regulators, rooting


Highbush blueberry (Vaccinium corymbosum L.) is increasingly farmed for its nutritional and health benefits, but high yield and fruit quality require proper planting material. Modified Murashige and Skoog (MW), Anderson’s Rhododendron (AN), and Woody Plant Medium (WPM) were compared for in vitro organogenesis and rooting of three highbush blueberries ‘Elizabeth’, ‘Meader’, and ‘Liberty’. All media contained 0.1 mg L-1 zeatin applied with a combination of IBA, IAA, and GA3. The results showed that MW medium is more suitable for in vitro multiplication of ‘Elizabeth’ and ‘Meader’, and WPM medium for ‘Liberty’. However, medium supplemented with a low concentration of IBA (≤ 0.4 mg L-1) and 0.1 mg L-1 zeatin increased the shoot regeneration rate of highbush blueberries multiplied in vitro. The rooting capability was studied by using WPM and AN medium with IBA and IAA with zeatin. The highest rooting rate (85%) and acclimatization (70%) were achieved in ‘Liberty’, and the lowest was in ‘Elizabeth’ (33.3% and 50%, respectively) and ‘Meader’ (33.3% and 43.8%, respectively). Rooted plantlets developed good quality roots and were transplanted into peat:perlite (4:1) substrates and acclimatized in a greenhouse under controlled conditions. We developed a complete micropropagation protocol for cvs. ‘Meader’, ‘Elizabeth’ and ‘Liberty’ blueberry. This protocol can be used for the production of certified vegetative material or different biotechnological purposes.


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How to Cite

FIGIEL-KROCZYŃSKA, M., KRUPA-MAŁKIEWICZ, M., & OCHMIAN, I. (2022). Efficient micropropagation protocol of three cultivars of highbush blueberry (Vaccinium corymbosum L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(4), 12856. https://doi.org/10.15835/nbha50412856



Research Articles
DOI: 10.15835/nbha50412856