Biolistic transformation of sugarcane (Saccharum officinarum L.) with the Oryza sativa L. H+-PPase gene for improved salt stress tolerance

Authors

  • Mazhar ULLAH University of Agriculture, Genomics and Bioinformatics Laboratory, Institute of Biotechnology and Genetic Engineering (IBGE), Peshawar 25000 Khyber Pakhtunkhwa (PK)
  • Mohammad S. KHAN University of Agriculture, Genomics and Bioinformatics Laboratory, Institute of Biotechnology and Genetic Engineering (IBGE), Peshawar 25000 Khyber Pakhtunkhwa (PK)
  • Asad JAN University of Agriculture, Genomics and Bioinformatics Laboratory, Institute of Biotechnology and Genetic Engineering (IBGE), Peshawar 25000 Khyber Pakhtunkhwa (PK)
  • Sayed U.A. SHAH University of Agriculture, Genomics and Bioinformatics Laboratory, Institute of Biotechnology and Genetic Engineering (IBGE), Peshawar 25000 Khyber Pakhtunkhwa (PK)
  • Navid IQBAL University of Agriculture, Genomics and Bioinformatics Laboratory, Institute of Biotechnology and Genetic Engineering (IBGE), Peshawar 25000 Khyber Pakhtunkhwa (PK)
  • Iqbal MUNIR University of Agriculture, Genomics and Bioinformatics Laboratory, Institute of Biotechnology and Genetic Engineering (IBGE), Peshawar 25000 Khyber Pakhtunkhwa (PK)

DOI:

https://doi.org/10.15835/nbha50312854

Keywords:

biolistic transformation, OVP1, sugarcane, salinity stress, tissue culture

Abstract

Several biotic and abiotic stresses adversely affect sugarcane crop. Amongst the abiotic stresses, salinity causes substantial losses to sugarcane yield. Lack of conducive environment for sugarcane flowering and unstable genome are the main constraints in sugarcane crop improvement via conventional breeding. Therefore, genetic engineering of sugarcane using Oryza sativa L. H+-PPase gene (OVP1) to confer salt tolerance is the preferred alternative strategy. In the present research, tissue culture and biolistic transformation protocols for sugarcane were optimized. For callus induction, Dichlorophenoxyacetic acid (2,4-D) was most effective. Maximum callus induction (50.22%) was achieved on CM-3 augmented with 2.5 mgL-1 2,4-D. The induced calli was subjected to biolistic transformation. For biolistic transformation, the gold particles (0.6 and 1.0 µm) and plasmid DNA (100 and 50 ng) were used for 1X and 2X bombardment. Significant differences (P≤0.05) were observed amongst the gold particles and plasmid DNA concentrations. Maximum transformation frequencies were observed when calli were bombarded 2X with 0.6 µm gold particles coated with 100 ng of DNA (23.60%). Selection and regeneration of transgenic calli were performed on MS media augmented with BAP (2.5 mgL-), NAA (1 mgL-1) hygromycin (25 mgL-1) and 2% coconut water. Regenerated plants after root induction on MS media containing NAA (2 mgL-1) and 2% coconut water were transferred to loam and clay (1:1) and acclimatized to greenhouse condition. The transgenic plants were confirmed through PCR and RT-PCR.

References

Ahire ML, Walunj PR, Kishor PBK, Nikam TD (2013). Effect of sodium chloride-induced stress on growth, proline, glycine betaine accumulation, antioxidative defence and bacoside A content in in vitro regenerated shoots of Bacopa monnieri (L.) Pennell. Acta Physiologia Plantarum 35(6):1943-1953. https://doi.org/10.1007/ s11738-013-1233

Altpeter F, Oraby H (2010). Sugarcane. In: Kempken F, Jung C (Eds). Genetic Modification of Plants, Biotechnology in Agriculture and Forestry, vol 64. Springer-Verlag, Berlin/Heidelberg. https://doi.org/10.1007/978-3-642-02391-0_23

Agrwal P, Agarwal P, Reddy MK, Sopory SK (2006). Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant Cell Reports 25:1263-1274. https://doi.org/10.1007/s00299-006-0204-8

Akhtar S, Wahid A, Rasul E (2003). Emergence, growth and nutrient composition of sugarcane sprouts under NaCl salinity. Biologia Plantarum 46:113-116. https://doi.org/10.1023/1022326604192

Basnayake SW, Moyle R, Birch RG (2011). Embryogenic callus proliferation and regeneration conditions for genetic transformation of diverse sugarcane cultivars. Plant Cell Reports 30(3):439-448. https://doi.org/10.1007/s00299-010-0927-4

Bower R, Birch RG (1992). Transgenic sugarcane plants via microprojectile bombardment. The Plant Journal 2(3):409-416.

Damayanti F, Tjahjoleksono A, Mariska I (2017). Agrobacterium tumefaciens-mediated transformation of MmCuZn-SOD gene to sugarcane (Saccharum officinarum cv. PS864) for acidic soil stress tolerance. International Journal of Agriculture and Biology 19(6):1489-1496. https://doi.org/10.1007/s00299-015-1831-8

FAO (2004). FAO production year book. Rome: FAO.

Fu X, Duc LT, Fontana S, Bong BB, Tinjuangjun P, Sudhakar D, Twyman RM, Christou P, Kohli A (2000). Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Research 9:11-1. https://doi.org/10.1023/A:1008993730505

Jackson MA, Anderson DJ, Birch RG (2013). Comparison of Agrobacterium and particle bombardment using whole plasmid or minimal cassette for production of high-expressing, low-copy transgenic plants. Transgenic Research 22(1):143-151. https://doi.org/10.1007/s11248-012-9639-6

Khan MS, Ahmad W, Ahmad MZ, Ullah M, Jan A (2021). Transformation of the OSC3H52 gene promoter in tissue culture of sugarcane. The Journal of Animal and Plant Sciences 31(1):156-165. https://doi.org/10.36899/JAPS.2021.1.0203

Kumar T, Khan MR, Abbas Z, Ali GM (2014). Genetic improvement of sugarcane for drought and salinity stress tolerance using Arabidopsis vacuolar pyrophosphatase (AVP1) gene. Molecular Biotechnology 56(3):199-209. https://doi.org/10.1007/s12033-013-9695-z

Kartha KK, Chibbar RN, Georges F, Leung N, Caswell K, Kendall E, Zureschi J (1989). Transient expression of chloramphenicol acetyltransferase (CAT) gene in barley cell cultures and immature embryos through microprojectile bombardment. Plant Cell Reports 8:429-432. https://doi.org/10.1007/BF00269041

Kumar T, Uzma, Khan MR, Abbas Z, Ali GM (2013). Efficient regeneration and genetic transformation of sugarcane with AVP1 gene. American-Eurasian Journal of Agricultural & Environmental Sciences 14 (2):165-171. https://doi.org/10.5829/idosi.aejaes.2014.14.02.12304

Kim JY, Gallo M, Altpeter F (2011). Analysis of transgene integration and expression following biolistic transfer of different quantities of minimal expression cassette into sugarcane (Saccharum spp. hybrids). Plant Cell Tissue and Organ Culture. https://doi.org/10.1007/s11240011-0043-

Lowe BA, Prakash NS, Way M, Mann MT, Spencer TM, Boddupalli RS (2009). Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA. Transgenic Research 18:831-840. https://doi.org/10.1007/s11248-009-9265-0

Lingle SE, Wiegand CL (1997). Soil salinity and sugarcane juice quality. Crop Research 54:259-268. https://doi.org/10.1016/S0378-4290(97)00058-0.

Murashige T, Skoog F (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15(3):473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Matroodi S, Motallebi M, Zamani MR, Mousavi A, Davoodi D, Moghaddassi-Jahromi Z (2013). Sugarcane (NCo310) transient transformation using uidA reporter gene. Iranian Journal of Biotechnology 11(2):89-95. https://doi.org/10.5812/IJB.11203

Mousavi M, Mousavi A, Habashi A, Arzani K (2009). Optimization of physical and biological parameters for transient expression of uidA gene in embryogenic callus of date palm (Phoenix dactylifera L.) via particle bombardment. African Journal of Biotechnology 8(16):3721-3730.

Perl A, Kless H, Blumenthal A, Galili G, Galun E (1992). Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization of culture conditions and DNA-microprojectile delivery procedures. Molecular Genetics 235:279-284. https://doi.org/10.1007/BF00279371

Potrykus I (1991). Gene transfer to plants: Assessment of published approaches and results. Annual Review of Plant Physiology and Plant Molecular Biology 42:205-225. https://doi.org/10.1146/annurev.pp.42.060191.001225

Ramasamy M, Mora V, Damaj MB, Padilla CS, Ramos N, Rossi D, Solís-Gracia N, Vargas-Bautista C, Irigoyen S, DaSilva JA (2018). A biolistic-based genetic transformation system applicable to a broad-range of sugarcane and energy cane varieties. GM Crops and Food 9(4): 211-227. https://doi.org/10.1080/21645698.2018.1553836

Randolph-Anderson B, Boynton JE, Dawson J, Dunder E, Eskes R, Gillham NW, Johnson A, Perlman PS, Suttie J, Heiser WC (2015). Sub-micron gold particles are superior to larger particles for efficient biolistic transformation of organelles and some cell types. BioRad Bull.

Reggiardo MI, Arana JL, Orsaria LM, Permingeat HR, Spitteler MA, Vallejos RH (1991). Transient transformation of maize tissues by micro projectile bombardment. Plant Science 75:237-243. https://doi.org/10.3389/fpls.2016.01949

Rogers SO, Bendich AJ (1988). Extraction of DNA from plant tissues. In: Gelvin SB, Schilperoort RA (Eds). Plant Molecular Biology Manual. (pp. A1–A10). Boston: Kluwer Academic Publishers

Saravanan S, Kumar KK, Raveendran M, Sudhakar D, Arul L, Kokiladevi E, Raguchander T, Mani S, Balasubramanian P (2018). Genetic engineering of sugarcane for drought and salt tolerant transgenic plants expressing the BcZAT12 Gene. International Journal of Current Microbiology and Applied Sciences 7(07):1594-1613. https://doi.org/10.20546/ijcmas.2018.707.188

Sivamani E, DeLong RK, Qu R (2009). Protamine-mediated DNA coating remarkably improves bombardment transformation efficiency in plant cells. Plant Cell Reports 28:213-221. https://doi.org/10.1007/s00299-008-0636-4

Sreeramanan S, Maziah M, Abdullah MP, Sariah M, Xavier R, Aini MFN (2005). Physical and biological parameters affecting transient GUS and GFP expression in banana via particle bombardment. Asia Pacific Journal of Molecular Biology and Biotechnology 13(1):35-57.

Sakakibara Y, Kobayashi H, Kasamo K (1996). Isolation and characterization of cDNAs encoding vacuolar H+ pyrophosphatase isoforms from rice (Oryza sativa L.). Plant Molecular Biology 31:1029-1038. https://doi.org/10.1007/BF00040721

Taparia Y, Gallo M, Altpeter F (2012). Comparison of direct and indirect embryogenesis protocols, biolistic gene transfer and selection parameters for efficient genetic transformation of sugarcane. Plant Cell Tissue and Organ Culture 111:131-141. https://doi.org/10.1007/s11240-012-0177-y

Sanford JC, Smith FD, Russell JA (1993). Optimizing the biolistic process for different biological applications. Methods Enzymology 217:483-509. https://doi.org/10.1016/0076-6879(93)17086-K

Ullah M, Khan MS (2022). Effects of carbon sources and synthetic growth regulators on the tissue culture of sugarcane. Sarhad Journal of Agriculture 38(1):312-321. https://dx.doi.org/10.17582/journal.sja/2022/38.1.312.321

Wu H, Doherty A, Jones HD (2009). Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos. In: Jones HD, Shewry PR (Eds). Transgenic Wheat, Barley and Oats. Series Methods in Molecular Biology Vol. 478. Humana Press; New Jersey, pp 93-103.

Xiong Y, Jung J, Zeng Q, Gallo M, Altpeter F (2013). Comparison of procedures for DNA coating of micro-carriers in the transient and stable biolistic transformation of sugarcane. Plant Cell Tissue and Organ Culture 112:95-99. https://doi.org/10.1007/s11240-012-0208-8

Zhang J, Li J, Wang X, Chen J (2010). OVP1, a vacuolar H+-translocating inorganic pyrophosphatase (V-PPase), overexpression improved rice cold tolerance. Plant Physiology and Biochemistry 49(1):33-38. https://doi.org/10.1016/j.plaphy.2010.09.014

Zuraida AR, Rahiniza K, Nurul Hafiza MR, Suri R, Zamri Z, Subramaniam S (2010). Factors affecting delivery and transient expression of gusA gene in Malaysian indica rice MR 219 callus via biolistic gun system. African Journal of Biotechnology 9(51):8810-8818. https://doi.org/10.5897/AJB10.1467

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Published

2022-12-06

How to Cite

ULLAH, M., KHAN, M. S., JAN, A., SHAH, S. U., IQBAL, N., & MUNIR, I. (2022). Biolistic transformation of sugarcane (Saccharum officinarum L.) with the Oryza sativa L. H+-PPase gene for improved salt stress tolerance . Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(4), 12854. https://doi.org/10.15835/nbha50312854

Issue

Vol. 50 No. 4 (2022)

Section

Research Articles
CITATION
DOI: 10.15835/nbha50312854

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Copyright (c) 2022 Mazhar ULLAH, Mohammad S. KHAN, Asad JAN, Sayed U.A. SHAH, Navid IQBAL, Iqbal MUNIR

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