Effect and mechanism of exogenous selenium on selenium content and quality of fresh tea leaves

Authors

  • Mu-fang SUN Xinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan Xinyang, Henan 464000; Anhui Agricultural University, State Key Laboratory of Tea Plant Biology and Utilization, 130 Changjiang West Road, Hefei, Anhui 230036 (CN)
  • Jing-jing WANG Xinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan Xinyang, Henan 464000 (CN)
  • Wei LIU Xinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan Xinyang, Henan 464000 (CN)
  • Peng YIN Xinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan Xinyang, Henan 464000 (CN)
  • Gui-yi GUO Xinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan Xinyang, Henan 464000 (CN)
  • Cui-ling TONG Jingzhou Institute of Technology, Jingzhou, Hubei 434025 (CN)
  • Ya-li CHANG Xinyang Agriculture and Forestry University, Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan Xinyang, Henan 464000 (CN)

DOI:

https://doi.org/10.15835/nbha50312814

Keywords:

antioxidase, mineral nutrient, selenium, tea, the leaves quality

Abstract

To study the effect and mechanism of selenium sources on the selenium content and quality of fresh tea leaves, tea seedlings (Camellia sinensis (L.) O. Kuntze) were the research object. A solution of 100 mg/L sodium selenate (Na2SeO4), sodium selenite (Na2SeO3), and selenium yeast (selenium yeast) were applied on the leaves surface of 5-week-old tea plants, and the selenium levels in the leaves, roots, and stems were determined at 20 weeks of age. The effects of different selenium sources on the mineral nutrient content, antioxidant enzyme activity, and quality parameters content in leaves were analyzed. The mechanism was analyzed by detecting the expression levels of related genes. The results showed that the three selenium sources can increase the growth of tea seedlings and the selenium content in leaves and stems, and the selenium yeast treatment had the most significant effect. Selenium spraying promoted the absorption of mineral nutrients such as nitrogen, phosphorus, and potassium, but had no significant impact on the absorption of calcium and magnesium. Spraying the three selenium sources dramatically increased the activities of APX, POD, and SOD antioxidant enzymes, among which the selenium yeast treatment had the most significant effect. However, there was no significant impact on the MDA level in this study. Selenium sources markedly increased leaves total amino acid levels, accompanied by up-regulation the genes of amino acid synthetic enzymes (CsGS, CsGOGAT, and CsGDH). Leaves glucose, tea polyphenol, total soluble protein, catechin, flavonoid contents, and sucrose were higher in selenium sources treatments than in control treatment seedlings. Moreover, selenium sources up-regulated expression of CsHMGR, CsAPX, and CsTCS1 genes. selenium yeast had the best comprehensive effect of the three selenium sources. These results confirmed that selenium sources play a positive role on the selenium content and quality of tea by increasing the antioxidant capacity of leaves, the absorption rate of mineral nutrients, and regulating expression of related genes in Camellia sinensis.

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References

Ahmed HS, Ahmed MF, Shoala T, Soliman M (2018). Impact of single or fractionated radiation and delenium nano-particles on acid lime (Citrus aurantifolia L.) seed germination ability and seedlings growth. Advances in Agriculture and Environmental Science: Open Access 1:91-100. https://doi.org/10.30881/aaeoa.00016

Babalar M, Mohebbi S, Zamani Z, Askari MA (2019). Effect of foliar application with sodium selenate on selenium biofortification and fruit quality maintenance of ‘Starking delicious’ apple during storage. Journal of the Science of Food and Agriculture 99:5149-5156. https://doi.org/10.1002/jsfa.9761

Bañuelos GS, Arroyo I, Pickering IJ, Yang SI, Freeman JL (2015). Selenium biofortification of broccoli and carrots grown in soil amended with se-enriched hyperaccumulator Stanleya pinnata. Food Chemistry 166:603-608. https://doi.org/10.1016/j.foodchem.2014.06.071

Chen Y, Jiang Y, Duan J, Shi J, Xue S, Kakuda Y (2010). Variation in catechin contents in relation to quality of ‘Huang Zhi Xiang’Oolong tea (Camellia sinensis) at various growing altitudes and seasons. Food Chemistry 119(2):648-652. https://doi.org/10.1016/j.foodchem.2009.07.014

Di MY, Chen X, Zhang XT, Liu Y, Seok I, Murugadoss V, ... Cui B (2021). Preparation of Selenium-rich tea set and its release properties of Selenium in water. ES Materials and Manufacturing 13:89-96. https://dx.doi.org/10.30919/esmm5f435

Du SP, Ma ZM, L X (2020). Effect of spraying with selenium fertilizer on yield, quality and nutrient absorption of watermelon in gravel-mulched field. Journal of Fruit Science 37:705-713. https://dx.doi.org/10.13925/j.cnki.gsxb.20190439

Eliseev MN, Alekseeva OM, Kosareva OA (2020). World tea market: Trends in production and consumption. International Trade and Trade Policy 2:131-145. https://dx.doi.org/10.21686/2410-7395-2020-2-131-145.

Gong TZ, Zhang DJ (2022). Effect of exogenous selenium application on selenium content and fruit quality as well as its physiological mechanism in walnut. Journal of Fruit Science 39(8):1443-1449. https://doi.org/10.13925/j.cnki.gsxb.20220373

Farooq MU, Tang ZC, Zeng R, Liang YK, Zhang YJ, Zheng T, ... Zhu JQ (2019). Accumulation mobilization and transformation of selenium in rice grain provided with foliar sodium selenite. Journal of the Science of Food and Agriculture 99:2892-2900. https://doi.org/10.1002/jsfa.9502

Gui JY, Rao S, Gou YY, Xu F, Cheng SY (2022). Comparative study of the effects of selenium yeast and sodium selenite on selenium content and nutrient quality in broccoli florets (Brassica oleracea L. var. italica). Journal of the Science of Food and Agriculture 102:1707-1718. https://doi.org/10.1002/jsfa.11511

Hawrylak-Nowak B (2015). Selenite is more efficient than selenate in alleviation of salt stress in lettuce plants. Acta Biologica Cracoviensia Series Botanica 57:49-54. https://doi.org/10.1515/abcsb-2015-0023

Hu Q, Xu J, Pang G (2003). Effect of selenium on the yield and quality of green tea leaves harvested in early spring. Journal of Agricultural and Food Chemistry 51:3379-3381. https://doi.org/10.1021/jf0341417

Jin JQ, Yao MZ, Ma CL, Ma JQ, Chen L (2016). Natural allelic variations of TCS1 play a crucial role in caffeine biosynthesis of tea plant and its related species. Plant Physiology and Biochemistry 100:18-26. https://doi.org/10.1016/j.plaphy.2015.12.020

Kápolna E, Hillestrøm PR, Laursen KH, Husted S, Larsen EH (2009). Effect of foliar application of selenium on its uptake and speciation in carrot. Food Chemistry 115:1357-1363. https://doi.org/10.1016/j.foodchem.2009.01.054

Li D, Zhou C, Zou N, Wu YL, Zhang JB, Pan CP (2021). Nanoselenium foliar application enhances biosynthesis of tea leaves in metabolic cycles and associated responsive pathways. Environmental Pollution 273:116503. https://doi.org/10.1016/j.envpol.2021.116503

Li YH, Lu JL, Fan FY, Shi YT (2014). Gene cloning and expression analysis of HMGR in tea plant roots. Journal of Tea Science 34:583-590. https://doi.org/10.13305/j.cnki.jts.2014.06.020

Liao DL, Su J, Tian YX (2021). Effects of bio-nano-selenium on citrus tree and fruit quality. Southern Horticulture 32:19-23. https://doi.org/10.3969/j.issn.1674-5868.2021.04.005 (in Chinese with English abstract).

Lin YX, Xin X, Yin GK, He JJ, Zhou YC, Lu XX (2019). Membrane phospholipids remodeling upon imbibition in Brassica napus L. seeds. Biochemical and Biophysical Research Communications 515:289-295. https://doi.org/10.1016/j.bbrc.2019.05.100

Lin ZH, Zhong QS, Chen CS (2012). Molecular cloning and quantitative analysis of GDH, GS and GOGAT genes from leave of tea plant. Journal of Tea Science 32:523-529. https://doi.org/10.13305/j.cnki.jts.2012.06.014

Liu K, Li S, Han J, Zeng X, Ling M, Mao J, … Jiang J (2021). Effect of selenium on tea (Camellia sinensis) under low temperature: Changes in physiological and biochemical responses and quality. Environmental and Experimental Botany 188:104475. https://doi.org/10.1016/j.envexpbot.2021.104475

Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and 2-ΔΔCT method. Methods 25:402-408. https://doi.org/10.1006/meth.2001.1262

Meucci A, Shiriaev A, Rosellini I, Malorgio F, Pezzarossa B (2021). Se-enrichment pattern, composition, and aroma profile of ripe tomatoes after sodium selenate foliar spraying performed at different plant developmental stages. Plants 10: 1050. https://doi.org/10.3390/plants10061050

Qiu H, Zhu X, Wan H, Xu L, Zhang Q, Hou P, ... Wen W (2020). Parallel Metabolomic and Transcriptomic analysis reveals key factors for quality improvement of tea plants. Journal of Agricultural and Food Chemistry 68(19):5483-5495. https://doi.org/10.1021/acs.jafc.0c00434

Rakgotho T, Ndou N, Mulaudzi T, Iwuoha E, Mayedwa N, Li MJ (2022). Green-synthesized zinc oxide nanoparticles mitigate salt stress in Sorghum bicolor. Agriculture 12:597. https://doi.org/10.3390/agriculture12050597

Saleem MF, Kamal MA, Shahid M, Saleem A, Shakeel A (2020). Exogenous selenium-instigated physiochemical transformations impart terminal heat tolerance in Bt cotton. Journal of Soil Science and Plant Nutrition 20: 274-283. https://doi.org/10.1007/s42729-019-00139-3

Schiavon M, Acqua SD, Mietto A, Pilon-Smits EAH, Sambo P, Malagoli M (2013). Selenium fertilization alters the chemical composition and antioxidant constituents of tomato (Solanum lycopersicon L.). Journal of Agricultural and Food Chemistry 61:10542-10554. https://doi.org/10.1021/jf4031822

Shao YD, Hu XC, Wu QS, Yang TY, Srivastava AK, Zhang DJ, ... Kuča K (2021). Mycorrhizas promote P acquisition of tea plants through changes in root morphology and P transporter gene expression. South African Journal of Botany 137:455-462. https://doi.org/10.1016/j.sajb.2020.11.028

Smits JE, Krohn RM, Akhtar E, Hore SK, Yunus M, Raqib R (2019). Food as medicine: Selenium enriched lentils offer relief against chronic arsenic poisoning in Bangladesh. Environmental Research 122:176-182. https://doi.org/10.1016/j.envres.2019.108561

Vinceti M, Filippini T, Wise LA (2018). Environmental selenium and human health: an update. Current Environmental Health Reports 5:464-485. https://doi.org/10.1007/s40572-018-0213-0

Wang N, Tan HY, Li S, Xu Y, Guo W, Feng Y (2017). Supplementation of micronutrient selenium in metabolic diseases: its role as an antioxidant. Oxidative Medicine and Cell Longevity 2017:7478523. https://doi.org/10.1155/2017/7478523

Wen MX, Wang P, Wu SH, Huang B (2022). Effects of spraying selenium at different stages on nutrient absorption and fruit quality of ‘Bendizao’ citrus. Journal of Zhejiang University 1: 29-35. https://doi.org/10.3785/j.issn.1008-9209.2021.04.012.

Xia XJ, Wang YJ, Zhou YH, Tao Y, Mao WH, Shi K, ... Yu JQ (2009). Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in Cucumber. Plant Physiology 150:801-814. https://doi.org/10.1104/pp.109.138230

Yang L, Zou YN, Tian ZH, Wu QS, Kuča K (2018). Effects of beneficial endophytic fungal inoculants on plant growth and nutrient absorption of trifoliate orange seedlings. Scientia Horticulturae 277:109815. https://doi.org/10.1016/j.scienta.2020.109815

Zhang DJ, Mao CM (2022). Effects of different selenium sources on selenium content, fruit quality, mineral contents and antioxidant activities of citrus. Journal of Yangtze University (Natural Science Edition) 19(5):114-119. https://doi.org/10.16772/j.cnki.1673-1409.2022.05.007

Zhang DJ, Yang YJ, Liu CY, Zhang F, Hu W, Wu QS (2018). Auxin modulates root-hair growth through its signaling pathway in citrus. Scientia Horticulturae 236:73-78. https://doi.org/10.1016/j.scienta.2018.03.038

Zhang HY, Han T, Tian L, Wang YN, Jia HJ (2010). Accumulation of Se in peach, jujube and strawberry after spraying Se fertilizer on leaves. Journal of Fruit Science 62:547-566. https://doi.org/10.1103/PhysRevD.62.054027

Zhang Y, Zhang B, Rui Y (2013). Study on selenium and other trace elements presents in selenium-enriched strawberry by icp-ms. Asian Journal of Chemistry 25:6451-6452. https://doi.org/10.14233/ajchem.2013.14210

Zhang YC, Wang P, Wu QH, Zou YN, Bao Q, Wu QS (2017). Arbuscular mycorrhizas improve plant growth and soil structure in trifoliate orange under salt stress. Archives of Agronomy and Soil Science 63:491-500. https://doi.org/10.1080/03650340.2016.1222609

Zhou F, Yang W, Wang M, Miao Y, Cui Z, Liang D (2018). Effects of selenium application on Se content and speciation in Lentinula edodes. Food Chemistry 265:182-188. https://doi.org/10.1016/j.foodchem.2018.05.087

Zhou X, Yang J, Kronzucker HJ, Shi W (2020). Selenium biofortification and interaction with other elements in plants: a review. Frontiers in Plant Science 11:586-421. https://doi.org/10.3389/fpls.2020.586421

Zhu JJ, Ma HJ A (2018). Effects of selenium on physiological characteristics of wine grape seedling under copper stress. Journal of Southern Agriculture 49:91-97. https://doi.org/10.3969/j.issn.2095-1191.2018.01.15

Zhu SM, Liang YL, Gao D, An XJ, Kong F (2017). Spraying foliar selenium fertilizer on quality of table grape (Vitis vinifera L.) from different source varieties. Scientia Horticulturae 218:87-94. https://doi.org/10.1016/j.scienta.2017.02.025

Zhu Z, Zhang Y, Liu J, Chen Y, Zhang X (2018). Exploring the effects of selenium treatment on the nutritional quality of tomato fruit. Food Chemistry 252:9-15. https://doi.org/10.1016/j.foodchem.2018.01.064

Zou YF, Xing DY, Li ZX, Yang WM, Xu SH, Yang L, ... Liu JP (2009). The effect of selenium ore fertilizer on main agronomic traits and production of cotton. Hubei Agricultural Sciences 48:2769-2701. https://doi.org/10.14088/j.cnki.issn0439-8114.2009.11.042 (in Chinese with English abstract).

Published

2022-09-06

How to Cite

SUN, M.- fang, WANG, J.- jing, LIU, W., YIN, P., GUO, G.- yi, TONG, C.- ling, & CHANG, Y.- li. (2022). Effect and mechanism of exogenous selenium on selenium content and quality of fresh tea leaves. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(3), 12814. https://doi.org/10.15835/nbha50312814

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Section

Research Articles
CITATION
DOI: 10.15835/nbha50312814