Foliar feeding of boron influencing biochemical attributes and enzyme activity in dragon fruit (Selenicereus monacanthus)
Keywords:antioxidant activity, boron, dragon fruit, enzyme activity, pollen germination
Boron plays crucial role in metabolic processes during fruit ripening and in turn ensures better fruit quality. However limited studies have been conducted to assess the influence of boron on fruit quality of dragon fruit. In the present study, the efficacy of boron was investigated on red-fleshed dragon fruit (Selenicereus monacanthus). Four levels of boron (100 mgL-1, 200 mgL-1, 300 mgL-1 and 400 mgL-1) were applied on 7- and 14-day-old flower buds. The highest pollen germinability, seed weight, fruit weight (274.32 ± 36.72g), pulp content (70.80 ± 1.79%) and pulp firmness (2.74 ± 0.18 N) were recorded when B was applied@300 mg L-1 on 7-day old flower bud. The same treatment also manifested higher soluble solid contents (17.42 ± 0.62 °Brix), sugar content, total carbohydrate (15.92 ± 1.12%), protein (1.33±0.11%), ascorbic acid (112.66 ± 4.98 µg/g), betacyanin (32.86±2.52 µg/g), total phenol (95.26 ± 3.72 µg GAE/ 100g), total flavonoid (37.65 ±2.14 mg QE/100g) and anti-oxidative activity (27.71±2.14 mM Fe II/100g). Correlation studies elucidated significant positive influence of pollen germinability on fruit weight, pulp content and pulp firmness. The activities of α-amylase, invertase and sucrose synthase enzymes were significantly upregulated with the application of B 300 mg L-1 on 7-day old flower bud. On the other hand, the activities of cell wall degrading enzymes such as cellulase, polygalacturonase and pectin methyl esterase were reduced with increasing levels of boron. The principal component analysis (PCA) illustrated the maximal proximity of most of the quality attributes with B 300 mgL-1, applied at 7-day old flower bud stage, thus exemplifying it as the best treatment.
Ali MS, Elhamahmy MA, El-Shiekh AF (2017). Mango trees productivity and quality as affected by boron and putrescine. Scientia Horticulturae 216:248-255. https://doi.org/10.1016/j.scienta.2017.01.026
Barr R, Bottger M, Crane FL (1993). The effect of boron on plasma membrane electron transport and associated proton secretion by cultured carrot cells. Biochemistry and Molecular Biology International 31(1):31-39.
Benzie IF, Strain JJ (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. In: Abelson J, Simon M, Verdine G, Pyle A (Eds). Methods in Enzymology. Academic Press, pp 15-27.
Blevins DG, Lukaszewski KM (1998). Boron in plant structure and function. Annual Review of Plant Biology 49(1):481-500. https://doi.org/10.1146/annurev.arplant.49.1.481
Brdar-Jokanović M (2020). Boron toxicity and deficiency in agricultural plants. International Journal of Molecular Sciences 21(4):1424. https://doi.org/10.3390/ijms21041424
Camacho‐Cristóbal JJ, Rexach J, González‐Fontes A (2008). Boron in plants: deficiency and toxicity. Journal of Integrative Plant Biology 50(10):1247-1255. https://doi.org/10.1111/j.1744-7909.2008.00742.x
Chang CC, Yang MH, Wen HM, Chern JC (2002). Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis 10(3):3. https://doi.org/10.38212/2224-6614.2748
Chormova D, Messenger DJ, Fry SC (2014). Rhamnogalacturonan-II cross-linking of plant pectins via boron bridges occurs during polysaccharide synthesis and/or secretion. Plant Signaling & Behavior 9(3):534-46. https://doi.org/10.4161/psb.28169
Dell B, Huang L, Bell RW (2002). Boron in plant reproduction. In: Goldbach HE, Brown PH, Rerkasem B, Thellier M, Wimmer MA, Bell RW (Eds). Boron in Plant and Animal Nutrition. Springer, Boston, MA pp. 103-117. https://doi.org/10.1007/978-1-4615-0607-2_9
Dorais M, Ehret DL, Papadopoulos AP (2008). Tomato (Solanum lycopersicum) health components: from the seed to the consumer. Phytochemistry Reviews 7(2):231-250. https://doi.org/10.1007/s11101-007-9085-x
El-Tantawy EM (2017). Improving growth, top root yield and betanin pigment of table beet as a result of spraying with magnesium, copper and boron under El-Arish region. Journal of Plant Production 8(2):231-238. https://doi.org/10.21608/jpp.2017.39612
Elwan MW, Elhamahmy MA (2015). Boron improved growth and fruit productivity of eggplant (Solanum melongana L.). Zagazig Journal of Agricultural Research 42(5):1101-1112.
Farajzadeh MA, Nagizadeh S (2002). Citric acid determination by dual wavelength spectrophotometry. Journal of the Chinese Chemical Society 49(4):619-624. https://doi.org/10.1002/jccs.200200095
Ganie MA, Akhter F, Bhat MA, Malik AR, Junaid JM, Shah MA, Bhat AH, Bhat TA (2013). Boron-a critical nutrient element for plant growth and productivity with reference to temperate fruits. Current Science 104(1):76-85. https://www.jstor.org/stable/24110665
Gilani SA, Basit A, Sajid M, Shah ST, Ullah I, Mohamed HI (2021). Gibberellic acid and boron enhance antioxidant activity, phenolic content, and yield quality in Pyrus communis L. Gesunde Pflanzen 73(4):395-406. https://doi.org/10.1007/s10343-021-00555-5
Hagerman AE, Austin PJ (1986). Continuous spectrophotometric assay for plant pectin methyl esterase. Journal of Agricultural and Food Chemistry 34(3):440-444. https://doi.org/10.1021/jf00069a015
Han S, Tang N, Jiang HX, Yang LT, Li Y, Chen LS (2009). CO2 assimilation, photosystem II photochemistry, carbohydrate metabolism and antioxidant system of citrus leaves in response to boron stress. Plant Science 176(1):143-153. https://doi.org/10.1016/j.plantsci.2008.10.004
Hu H, Brown PH (1994). Localization of boron in cell walls of squash and tobacco and its association with pectin (evidence for a structural role of boron in the cell wall). Plant Physiology 105(2):681-689. https://doi.org/10.1104/pp.105.2.681
Ikram EH, Eng KH, Jalil AM, Ismail A, Idris S, Azlan A, Nazri HS, Diton NA, Mokhtar RA (2009). Antioxidant capacity and total phenolic content of Malaysian underutilized fruits. Journal of Food Composition and Analysis 22(5):388-393. https://doi.org/10.1016/j.jfca.2009.04.001
Kamiloglu O (2011). Influence of some cultural practices on yield, fruit quality and individual anthocyanins of table grape cv. ‘Horoz Karasi’. Journal of Animal and Plant Sciences 21(2):240-245.
Kapur A, Hasković A, Čopra-Janićijević A, Klepo L, Topčagić A, Tahirović I, Sofić E (2012). Spectrophotometric analysis of total ascorbic acid content in various fruits and vegetables. Bulletin of the Chemists and Technologists of Bosnia and Herzegovina 38(4):39-42.
Khalaj K, Ahmadi N, Souri MK (2016). Improvement of postharvest quality of Asian pear fruits by foliar application of boron and calcium. Horticulturae 3(1):15. https://doi.org/10.3390/horticulturae3010015
Kishore K (2016). Phenological growth stages of dragon fruit (Hylocereus undatus) according to the extended BBCH-scale. Scientia Horticulturae 213:294-302. https://doi.org/10.1016/j.scienta.2016.10.047
Korkmaz N, Aşkın MA, Ercişli S, Okatan V (2016). Foliar application of calcium nitrate, boric acid and gibberellic acid affects yield and quality of pomegranate (Punica granatum L.). Acta Scientiarum Polonorum Hortorum Cultus 15(3):105-112. https://hdl.handle.net/20.500.12809/2795
Korotkova N, Borsch TH, Arias S (2017). A phylogenetic framework for the Hylocereeae (Cactaceae) and implications for the circumscription of the genera. Phytotaxa 327(1):1-46. https://doi.org/10.11646/phytotaxa.327.1.1
Lim SD, Yusof YA, Chin NL, Talib RA, Endan J, Aziz MG (2011). Effect of extraction parameters on the yield of betacyanins from pitaya fruit (Hylocereus polyrhizus) pulps. Journal of Food Agriculture and Environment 9(2):158-162. https://doi.org/10.1234/4.2011.2079
Lima LC, Chitarra AB, Chitarra MI (2001). Changes in amylase activity starch and sugars contents in mango fruits pulp cv. Tommy Atkins with spongy tissue. Brazilian Archives of Biology and Technology 44:59-62. https://doi.org/10.1590/S1516-89132001000100008
Lohani S, Trivedi PK, Nath P (2004). Changes in activities of cell wall hydrolases during ethylene-induced ripening in banana: effect of 1-MCP, ABA and IAA. Postharvest Biology and Technology 31(2):119-126. https://doi.org/10.1016/j.postharvbio.2003.08.001
Marschner P. (2011). Marschner's mineral nutrition of higher plants. Academic press (3rd Ed.), London.
Metz C, Nerd A, Mizrahi Y (2000). Viability of pollen of two fruit crop cacti of the genus Hylocereus is affected by temperature and duration of storage. HortScience 35(2):199-201. https://doi.org/10.21273/HORTSCI.35.2.199
Miller GL (1959). Use of Dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3):426-428. https://doi.org/10.1021/ac60147a030
Mohan T, Rajesh PN, Zuhra FK, Vijitha K (2014). Magnitude of changes in the activity of amylase and cellulase and its association with the biochemical composition during maturation and ripening of banana (Musa spp.). Biochemistry and Physiology 3:127.
Mousavi SM, Motesharezadeh B (2020). Boron deficiency in fruit crops. In: Srivastava AK, Hu C (Eds). Fruit Crops. Elsevier, pp 191-209.
Muengkaew R, Whangchai K, Chaiprasart P (2018). Application of calcium–boron improves fruit quality, cell characteristics, and effective softening enzyme activity after harvest in mango fruit (Mangifera indica L.). Horticulture, Environment and Biotechnology 59(4):537-546. https://doi.org/10.1007/s13580-018-0059-2
Nielsen SS (2017). Total carbohydrate by phenol-sulfuric acid method. Food Analysis Laboratory Manual, Springer.
Nyomora AM, Brown PH, Pinney K, Polito VS (2000). Foliar application of boron to almond trees affects pollen quality. Journal of the American Society for Horticultural Science 125(2):265-270. https://doi.org/10.21273/JASHS.125.2.265
Panda BB, Badoghar AK, Das K, Panigrahi R, Kariali E, Das SR, Dash SK, Shaw BP, Mohapatra PK (2015). Compact panicle architecture is detrimental for growth as well as sucrose synthase activity of developing rice kernels. Functional Plant Biology 42(9):875-887. https://doi.org/10.1071/FP14363
Perween T, Mandal KK, Hasan MA (2018). Dragon fruit: An exotic super future fruit of India. Journal of Pharmacognosy and Phytochemistry 7(2):1022-1026.
Pfeffer H, Dannel F, Romheld V (1998). Are there connections between phenol metabolism, ascorbate metabolism and membrane integrity in leaves of boron‐deficient sunflower plants? Physiologia Plantarum 104(3):479-485. https://doi.org/10.1034/j.1399-3054.1998.1040325.x
Popper ZA, Fry SC (2008). Xyloglucan− pectin linkages are formed intra-protoplasmically, contribute to wall-assembly, and remain stable in the cell wall. Planta 227(4):781-794. https://doi.org/10.1007/s00425-007-0656-2
Priatni S, Pradita A (2015). Stability study of betacyanin extract from red dragon fruit (Hylocereus polyrhizus) peels. Procedia Chemistry 16:438-44. https://doi.org/10.1016/j.proche.2015.12.076
Shi YC, Sun B, Liu WQ (2012). Sucrose phosphate synthase plays a key role in boron‐promoted sucrose synthesis in tobacco leaves. Journal of Plant Nutrition and Soil Science 175(6):854-859. https://doi.org/10.1002/jpln.201100423
Shireen F, Nawaz MA, Chen C, Zhang Q, Zheng Z, Sohail H, Sun J, Cao H, Huang Y, Bie Z (2018). Boron: functions and approaches to enhance its availability in plants for sustainable agriculture. International Journal of Molecular Sciences 19(7):1856. https://doi.org/10.3390/ijms19071856
Stino RG, Abd El-Wahab SM, Habashy SA, Kelani RA (2011). Productivity and fruit quality of three mango cultivars in relation to foliar sprays of calcium, zinc, boron or potassium. Journal of Horticultural Science & Ornamental Plants 3(2):91-98.
Storey JB (2007). Zinc. In: Barker AV, Pilbeam DJ (Eds). Handbook of Plant Nutrition, CRC Press, New York.
Topcu H, Degirmenci I, Sonmez DA, Paizila A, Karci H, Kafkas S, Kafkas E, Ercisli S, Alatawi A (2022). Sugar, invertase enzyme activities and invertase gene expression in different developmental stages of strawberry fruits. Plants 11(4):509. https://doi.org/10.3390/plants11040509
van Handel E (1968). Direct micro determination of sucrose. Analytical Biochemistry 22(2):280-283. https://doi.org/10.1016/0003-2697(68)90317-5
Westmark PR, Gardiner SJ, Smith BD (1996). Selective monosaccharide transport through lipid bilayers using boronic acid carriers. Journal of the American Chemical Society 118(45):11093-11100. https://doi.org/10.1021/ja961264h
Wichienchot S, Jatupornpipat M, Rastall RA (2010). Oligosaccharides of pitaya (dragon fruit) flesh and their prebiotic properties. Food Chemistry 120(3):850-857. https://doi.org/10.1016/j.foodchem.2009.11.026
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Copyright (c) 2022 Ankita SAHU, Kundan KISHORE, Satya N. DASH, Sarat C. SAHOO, Rabi K. NAYAK, Bijay K. BAIDYA, Kuldeep K. SHUKLA, Subhashree BARIK
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