Characterization of ‘Cabernet Sauvignon’ pomace extracts and evaluation of antifungal potential of Alternaria sp. and Fusarium sp.

Authors

  • Alin DIN University of Craiova, Faculty of Agriculture and Horticulture, 13 A.I. Cuza Street, Craiova, Dolj; University of Pitesti, Regional Center of Research & Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, 11 Doaga Street, 110440 Pitesti, Arges (RO)
  • Denisa Ș. VILCOCI University of Pitesti, Regional Center of Research & Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, 11 Doaga Street, 110440 Pitesti, Arges (RO)
  • Georgiana CIRSTEA University of Pitesti, Regional Center of Research & Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, 11 Doaga Street, 110440 Pitesti, Arges (RO)
  • Denis NEGREA University of Pitesti, Regional Center of Research & Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, 11 Doaga Street, 110440 Pitesti, Arges (RO)
  • Sorin MOGA University of Pitesti, Regional Center of Research & Development for Materials, Processes and Innovative Products Dedicated to the Automotive Industry, 11 Doaga Street, 110440 Pitesti, Arges (RO)
  • Cristina MIHAESCU University of Pitesti, Faculty of Science, Physical Education and Informatics, Department of Natural Sciences, Targu din Vale Street, 110040 Pitesti, Arges (RO)
  • Rodi MITREA University of Craiova, Faculty of Agriculture and Horticulture, 13 A.I. Cuza Street, Craiova, Dolj (RO)

DOI:

https://doi.org/10.15835/nbha50312774

Keywords:

Alternaria sp., antifungal activity, 'Cabernet Sauvignon' pomace, extraction method, Fusarium sp., polyphenols

Abstract

This study was conducted to capitalize on the waste produced by the vinification process which proved to be important sources of bioactive compounds with significant antifungal properties. ‘Cabernet Sauvignon’ grape pomace extracts were characterized in terms of total polyphenol content, antioxidant potential, but also evaluated in terms of antifungal effect against phytopathogenic fungi (Fusarium sp. and Alternaria sp.). The isolates used in this study were grown on three potato-dextrose-agar culture media, Czapek Dox and Malt-Agar. The highest amount of polyphenols was determined from the extract obtained by the microwave-assisted method (42.76 mg/g GAE), followed by the extract obtained by maceration (30.37 mg/g GAE). The lowest amount was obtained by the ultrasound-assisted method (15.06 mg/g GAE). However, the highest antioxidant activity was determined in the macerated extract, TEAC = 0.0523. The results of in vitro tests clearly indicated a high inhibitory percentage on the mycelium growth rate and, respectively, a significant decrease in spore germination power in Fusarium sp. (91.56%). However, for the pathogen Alternaria sp. further studies are needed to correctly validate the percentage of inhibition, as alcohol has been shown to have a negative effect on it.

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Published

2022-09-27

How to Cite

DIN, A., VILCOCI, D. Ș., CIRSTEA, G., NEGREA, D., MOGA, S., MIHAESCU, C., & MITREA, R. (2022). Characterization of ‘Cabernet Sauvignon’ pomace extracts and evaluation of antifungal potential of Alternaria sp. and Fusarium sp. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(3), 12774. https://doi.org/10.15835/nbha50312774

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Research Articles
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DOI: 10.15835/nbha50312774