Responses of wheat and barley to Acacia saligna leaf and stem extracts: influence on growth and ascorbate-glutathione cycle

Authors

  • Haifa Abdulaziz Sakit ALHAITHLOUL Jouf University, College of Science, Biology Department, Sakaka 2014 (EG)
  • Mona H. SOLIMAN Cairo University, Faculty of Science, Botany and Microbiology Department, Giza 12613; Taibah University, Faculty of Science, Biology Department, Al-Sharm, Yanbu El-Bahr, Yanbu 46429 (EG)

DOI:

https://doi.org/10.15835/nbha50212709

Keywords:

Acacia saligna, allelopathy, ascorbate-glutathione cycle, barley, lipoxygenase, wheat

Abstract

The present study aimed to study the effect of dry leaf and stem leachates of Acacia saligna on wheat’s growth and enzyme functioning (Triticum aestivum) and barley (Hordeum vulgare). Leaf leachates (LL) and stem leachates (SL) of A. saligna were applied through root and nutrient solution in different concentrations i.e., 5, 10, and 15%. Treatment of LL and SL declined the growth in terms of height and dry weight in both tested plants in concentration-dependent manner with the maximal decline due to 15% LL. In addition, content of relative water, total chlorophylls, and carotenoids decreased in both wheat as well as barley. The activity of ascorbate peroxidase, monodehydro ascorbate reductase, dehydroascorbate reductase, and glutathione reductase increased considerably due to the treatment of LL and SL. The indigenous tolerance mechanisms in wheat and barley seedlings were further strengthened in wheat and barley by increased accumulation of glycine betaine, glutathione, and ascorbate in response to LL and SL treatment. Additionally, the activity of lipoxygenase and protease were increased significantly due to LL and SL treatment with a maximal increase at higher concentrations. From the present study it can be concluded that extracts of leaf and stem of A. saligna inhibit the growth of wheat and barley significantly with a concomitant increase in the functioning of the ascorbate-glutathione (AsA-GSH) cycle. Further, both crop species showed comparable responses to A. saligna leachates.

References

Ahanger MA, Agarwal RM (2017). Potassium up-regulates antioxidant metabolism and alleviates growth inhibition under water and osmotic stress in wheat (Triticum aestivum L). Protoplasma 254(4):1471-1486. https://doi.org/10.1007/s00709-016-1037-0

Ahanger MA, Qin C, Begum N, Maodong Q, Dong XX, El-Esawi M, … Zhang L (2019). Nitrogen availability prevents oxidative effects of salinity on wheat growth and photosynthesis by up-regulating the antioxidants and osmolytes metabolism, and secondary metabolite accumulation. BMC Plant Biology 19:479 https://doi.org/10.1186/s12870-019-2085-3

Ahanger MA, Tomar NS, Tittal M, Argal S, Agarwal RM (2017). Plant growth under water/ salt stress: ROS production; antioxidants and significance of added potassium under such conditions. Physiology and Molecular Biology of Plants 23(4about:blank):731-744. https://doi.org/10.1007/s12298-017-0462-7

Ahmad P, Alam P, Balawi TH, Altalayan FH, Ahanger MA, Ashraf M (2020). Sodium nitroprusside (SNP) improves tolerance to arsenic (As) toxicity in Vicia faba through the modifications of biochemical attributes, antioxidants, ascorbate-glutathione cycle and glyoxalase cycle. Chemosphere 244:125480. https://doi.org/10.1016/j.chemosphere.2019.125480

Ahmad P, Alyemeni MN, Wijaya L, Ahanger MA, Ashraf M, Alam P, … Rinklebe J (2021). Nitric oxide donor, sodium nitroprusside, mitigates mercury toxicity in different cultivars of soybean. Journal of Hazardous Materials 408:124852. https://doi.org/10.1016/j.jhazmat.2020.124852

Alhaithloul HA, Soliman MH, Ameta KL, El-Esawi MA, Elkelish A (2019). Changes in ecophysiology, osmolytes, and secondary metabolites of the medicinal plants of Mentha piperita and Catharanthus roseus subjected to drought and heat stress. Biomolecules 10(1):43. https://doi.org/10.3390/biom10010043

Ali S, Abbas Z, Seleiman MF, Rizwan M, Yavaş I, Alhammad BA, Shami A, Hasanuzzaman M, Kalderis D (2020). Glycine betaine accumulation, significance and interests for heavy metal tolerance in plants. Plants 9(7):896. https://doi.org/10.3390/plants9070896

Anjum NA, Chan MT, Umar S (2010). Ascorbate-glutathione pathway and stress tolerance in plants. Springer Science & Business Media. https://link.springer.com/book/10.1007/978-90-481-9404-9

Araniti F, Costas-Gil A, Cabeiras-Freijanes L, Lupini A, Sunseri F, Reigosa MJ, Abenavoli MR, Sánchez-Moreiras AM (2018). Rosmarinic acid induces programmed cell death in Arabidopsis seedlings through reactive oxygen species and mitochondrial dysfunction. PloS One 12:e0208802. https://doi.org/10.1371/journal.pone.0208802

Araniti F, Lupini A, Sunseri F, Abenavoli MR (2017). Allelopatic potential of Dittrichia viscosa (L.) W. Greuter mediated by VOCs: A physiological and metabolomic approach. PLoS One 12(1):e0170161 https://doi.org/10.1371/journal.pone.0170161

Arnon DI (1949). Copper enzymes in isolated chloroplast polyphenol oxidase in Beta vulgaris. Plant Physiology 24:1-15. https://doi.org/10.1104/pp.24.1.1

Ashraf M, Foolad R (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59(2):206-216. https://doi.org/10.1016/j.envexpbot.2005.12.006

Bogatek R, Gniazdowska A (2007). ROS and phytohormones in plant-plant allelopathic interaction. Plant Signaling and Behaviour 2(4):317-318. https://doi.org/10.4161/psb.2.4.4116

Cai SL, Mu XQ (2012). Allelopathic potential of aqueous leaf extracts of Datura stramonium L. on seed germination, seedling growth and root anatomy of Glycine max (L.) Merrill. Allelopathy Journal 30:235-245. https://doi.org/10.3923/pjbs.2018.187.198

Chen SSY, Chi WC, Trinh NN, Cheng KT, Chen YA, Lin TC, … Chiang TY (2015) Alleviation of allelochemical juglone-induced phytotoxicity in tobacco plants by proline. Journal of Plant Interactions 10:167-172. https://doi.org/10.1080/17429145.2015.1045946

Chopra N, Tewari G, Tewari LM, Upreti B, Pandey N. 2017. Allelopathic effect of Echinochloa colona L. and Cyperus iria L. weed extracts on the seed germination and seedling growth of rice and soyabean. Advances in Agriculture. Article ID 5748524, https://doi.org/10.1155/2017/5748524

Cruz-Ortega R, Lara-Nunez A, Anaya AL (2007). Allelochemical stress can trigger oxidative damage in receptor plants. mode of action of phytotoxicity. Plant Signaling and Behaviour 2(4):269-270. https://doi.org/10.4161/psb.2.4.3895

Diaz-Mendoza M, Velasco-Arroyo B, Gonzalez-Melendi P, Martinez M, Diaz I (2014). C1A cysprot-cystatin interactions in leaf senescence. Journal of Experimental Botany 65:3825-3833. https://doi.org/10.1093/jxb/eru043

Ding H, Ali A, Cheng Z (2019) An allelopathic role for garlic root exudates in the regulation of carbohydrate metabolism in cucumber in a hydroponic co-culture system. Plants 9(1):45. https://doi.org/10.3390/plants9010045

Doderer A, Kokkelink I, van der Veen S, Valk B, Schram A, Douma A (1992) Purification and characterization of two lipoxygenase isoenzymes from germinating barley. Biochimica et Biophysica Acta 112:97-104. https://doi.org/10.1016/0167-4838(92)90429-H

El-Gawad AAM, El-Amier YA (2015). Allelopathy and potential impact of invasive Acacia saligna (Labill.) Wendl. on plant diversity in the Nile delta coast of Egypt. International Journal of Environmental Research 9(3):923-932. http://dx.doi.org/10.4067/S0718-58392020000300452

Elisante F, Tarimo M, Ndakidemi P (2013). Allelopathic effect of seed and leaf aqueous extracts of Datura stramonium on leaf chlorophyll content, shoot and root elongation of Cenchrus ciliaris and Neonotonia wightii. American Journal of Plant Sciences 4(12):2332-2339. https://doi.org/10.4236/ajps.2013.412289

Ellman GL (1959) Tissue sulphydryl groups. Archives of Biochemistry and Biophysics 82:70‐77. https://doi.org/10.1016/0003-9861(59)90090-6

Foyer CH, Halliwell B (1976). The presence of glutathione and glutathione reductase in chloroplast: a proposed role in ascorbic acid metabolism. Planta 133:21-25. https://doi.org/10.1007/BF00386001

Foyer CH, Noctor G (2011). Ascorbate and glutathione: The heart of the redox hub. Plant Physiology 155:2-18. https://doi.org/10.1104/pp.110.167569

Ghimire BK, Hwang MH, Sacks EJ, Yu CY, Kim SH, Chung IM (2020). Screening of allelochemicals in miscanthus sacchariflorus extracts and assessment of their effects on germination and seedling growth of common weeds. Plants 9:1313. https://doi.org/10.3390/plants9101313

Gomez-Sanchez A, Gonzalez-Melendi P, Santamaria ME, Arbona V, Lopez-Gonzalvez A, Garcia A, … Diaz I (2019). Repression of drought-induced cysteine-protease genes alter barley leaf structure and the response to abiotic and biotic stresses. Journal of Experimental Botany 70:2143-2155. https://doi.org/10.1093/jxb/ery410

Green NM, Neurath H (1954). Proteolytic enzymes. In: Neurath H, Vailey K (Eds). The Proteins. vol. II. Part B, Academic Press, New York, pp 1057-1198. https://doi.org/10.1016/B978-0-12-395721-4.50011-1

Grieve CM, Grattan SR (1983). Rapid assay for determination of water-soluble quaternary ammonium compounds. Plant Soil 70:303-307. https://doi.org/10.1007/BF02374789

Hallak AMG, Davide LC, Souza IF (1999). Effects of sorghum (Sorghum bicolor L.) root exudates on the cell cycle of the bean plant (Phaseolus vulgaris L.) root. Genetics and Molecular Biology 22:95-99. https://doi.org/10.1590/S1415-47571999000100018

Hasanuzzaman M, Bhuyan MHMB, Anee TI, Parvin K, Nahar K, Mahmud JA, Fujita M (2019). Regulation of ascorbate-glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants (Basel). 8(9):384. https://doi.org/10.3390/antiox8090384

Hasanuzzaman M, Borhannuddin Bhuyan MHM, Zulfiqar F, Raza A, Mohsin SM, Mahmud JA, … Fotopoulos V (2020). Reactive oxygen species and antioxidant defense in plants under abiotic stress: revisiting the crucial role of a universal defense regulator. Antioxidants 9:681. https://doi.org/10.3390/antiox9080681

Hasanuzzaman M, Hossain MA, Fujita M (2011) Nitric oxide modulates antioxidant defense and the methylglyoxal detoxification system and reduces salinity-induced damage of wheat seedlings. Plant Biotechnology Reports 5(4):353-365. https://doi.org/10.1007/s11816-011-0189-9

Hasanuzzaman M, Hossain MA, Fujita M (2012). Exogenous selenium pretreatment protects rapeseed seedlings from cadmium-induced oxidative stress by up-regulating antioxidant defense and methylglyoxal detoxification systems. Biological Trace Element Research 149:248-261. https://doi.org/10.1007/s12011-012-9419-4

Hasanuzzaman M, Nahar K, Rahman A, Mahmud JA, Alharby HF, Fujita M (2018). Exogenous glutathione attenuates lead-induced oxidative stress in wheat by improving antioxidant defense and physiological mechanisms. Journal of Plant Interactions 13:203-212. https://doi.org/10.1080/17429145.2018.1458913

Hoque TS, Hossain MA, Mostofa MG, Burritt DJ, Fujita M, Tran L-SP (2016) Methylglyoxal: an emerging signaling molecule in plant abiotic stress responses and tolerance. Frontiers in Plant Science 7:1341. https://doi.org/10.3389/fpls.2016.01341

Hossain MA, Nakano Y, Asada K (1984). Monodehydroascorbate reductase in spinach chloroplasts and its participation in the regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiology 25:385-395. https://doi.org/10.1093/oxfordjournals.pcp.a076726

Huang CZ, Xu L, Jin-Jing S,Zhang ZH, Fu ML, Teng HY, Yi KK (2020). Allelochemical p-hydroxybenzoic acid inhibits root growth via regulating ROS accumulation in cucumber (Cucumis sativus L.). Journal of Integrative Agriculture 19(2):518-527. https://doi.org/10.1016/S2095-3119(19)62781-4

Huang P, He L, Abbas A, Hussain S, Hussain S, Du D, … Saqib M (2021). Seed priming with sorghum water extract improves the performance of camelina (Camelina sativa (L.) Crantz.) under salt stress. Plants (Basel) 10(4):749. https://doi.org/10.3390/plants10040749

Hussain MI, Reigosa MJ (2011) Allelochemical stress inhibits growth, leaf water relations, PSII photochemistry, non-photochemical fluorescence quenching, and heat energy dissipation in three C3 perennial species. Journal of Experimental Botany 62(13):4533-4545. https://doi.org/10.1093/jxb/err161

Inderjit Duke SO (2003). Ecophysiological aspects of allelopathy. Planta 217:529-539. https://doi.org/10.1007/s00425-003-1054-z

Javaid A, Shafique S, Bajwa R, Shafique S (2006). Effect of aqueous extracts of allelopathic crops on germination and growth of Parthenium hysterophorus L. South African Journal of Botany 72(4):609-612. https://doi.org/10.1016/j.sajb.2006.04.006

Kumbhar BA, Patel DD (2016). Weed and its management: a major threat to crop economy. Journal Pharmaceutical Science and Bioscientific Research (JPSBR) 6(6):801-805. http://eprints.icrisat.ac.in/id/eprint/14711

Lara-Nuñez Ma, Romero-Romero T, Ventura JL, Blancas V, Anaya Al, Cruz-Ortega R (2006). Allelochemical stress causes inhibition of growth and oxidative damage in Lycopersicon esculentum. Plant, Cell and Environment 29:2009-2016. https://doi.org/10.1111/j.1365-3040.2006.01575.x

Lawson T (2009). Guard cell photosynthesis and stomatal function. New Phytologist 181(1):13-34. https://doi.org/10.1111/j.1469-8137.2008.02685.x

Li, J., Chen, L., Chen, Q, Miao Y, Peng Z, Huang B, Guo L, Liu D, Du H (2021). Allelopathic effect of Artemisia argyi on the germination and growth of various weeds. Scientific Reports 11:4303. https://doi.org/10.1038/s41598-021-83752-6

Liu H, Hu M, Wang Q, Cheng L, Zhang Z (2018). Role of papain-like cysteine proteases in plant development. Frontiers in Plant Science 9:1717. https://doi.org/10.3389/fpls.2018.01717

Ma H, Chen Y, Chen J, Zhang Y, Zhang T, He H (2020). Comparison of allelopathic effects of two typical invasive plants: Mikania micrantha and Ipomoea cairica in Hainan Island. Scientific Reports 10:11332. https://doi.org/10.1038/s41598-020-68234-5

Maqbool N, Wahid A, Farooq M, Cheema ZA, Siddique KHM (2013). Allelopathy and abiotic stress interaction in crop plants. In: Cheema Z, Farooq M, Wahid A (Eds). Allelopathy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-30595-5_19

Martinez M, Gómez-Cabellos S, Giménez MJ, Barro F, Diaz I, Diaz-Mendoza M (2019). Plant proteases: from key enzymes in germination to allies for fighting human gluten-related disorders. Frontiers in Plant Science 10:721. https://doi.org/10.3389/fpls.2019.00721

MasoodA, Per TS, Asgher M, Fatma M, Khan MIR, Rasheed F, Hussain SJ, Khan NA (2016). Glycine betaine: role in shifting plants toward adaptation under extreme environments. In: Iqbal N, Nazar RA, Khan N (Eds). Osmolytes and Plants Acclimation to Changing Environment: Emerging Omics Technologies. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2616-1_5

Mir RA, Argal S, Agarwal RM (2018). Accumulation of secondary metabolites and osmotica in different parts of Tagetes erecta L. and its ecophysiological relevance. International Journal Scientific Research and Reviews 7:198-209. https://www.ijsrr.org/down_NCRIS18.php

Mir RA, Argal S, Ahanger MA, Tomar NS, Agarwal RM (2021). Variation in phenolic compounds, antioxidant activity and osmotica of different cultivars of Tagetes erecta L. at Different growth stages and effect of its leachates on germination and growth of wheat (Triticum aestivum L.). Journal of Plant Growth Regulation. https://doi.org/10.1007/s00344-021-10348-9

Mukherjee SP, Choudhuri MA (1983). Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiologia Plantarum 58:166-170. https://doi.org/10.1111/j.1399-3054.1983.tb04162.x

Nahar K, Hasanuzzaman M, Alam MM, Rahman A, Suzuki T, Fujita M (2016). Polyamine and nitric oxide crosstalk: antagonistic effects on cadmium toxicity in mung bean plants through upregulating the metal detoxification, antioxidant defense and methylglyoxal detoxification systems. Ecotoxicology and Environmental Safety 126:245-255. https://doi.org/10.1016/j.ecoenv.2015.12.026

Nahar K, Hasanuzzaman M, Suzuki T, Fujita M (2017) Polyamines-induced aluminum tolerance in mung bean: A study on antioxidant defense and methylglyoxal detoxification systems. Ecotoxicology 26(1):58-73. https://doi.org/10.1007/s10646-016-1740-9

Nakano Y, Asada K (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach-chloroplasts. Plant Cell Physiology 22:867-880. https://doi.org/10.1093/oxfordjournals.pcp.a076232

Naz H, Akram NA, Ashraf M (2016). Impact of ascorbic acid on growth and some physiological attributes of cucumber (Cucumis sativus) plants under water-deficit conditions. Pakistan Journal of Botany 48:877-883. https://www.pakbs.org/pjbot/PDFs/48(3)/05.pdf

Omezzine F, Ladhari A, Haouala R (2014). Physiological and biochemical mechanisms of allelochemicals in aqueous extracts of diploid and mixoploid Trigonella foenum-graecum L. South African Journal of Botany 93:167-178. https://doi.org/10.1016/j.sajb.2014.04.009

Rosahl S (1996). Lipoxygenases in plants--their role in development and stress response. Zeitschrift für Naturforschung C 51(3-4):123-38. https://doi.org/10.1515/znc-1996-3-401

Sadiq Y, Zaid A, Khan MMA (2020). Adaptive physiological responses of plants under abiotic stresses: role of phytohormones. In: Hasanuzzaman M (Ed.) Plant Ecophysiology and Adaptation under Climate Change: Mechanisms and Perspectives I. Springer, Singapore. https://doi.org/10.1007/978-981-15-2156-0_28

Scavo A, Abbate C, Mauromicale G (2019). Plant allelochemicals: agronomic, nutritional and ecological relevance in the soil system. Plant Soil 442:23-48. https://doi.org/10.1007/s11104-019-04190-y

Shah RH, Baloch MS, Zubair M, Khan EA (2017). Phytotoxic effect of aqueous extracts of different plant parts of milkweed on weeds and growth and yield of wheat. Planta Daninh v35:e017168160. https://doi.org/10.1590/S0100-83582017350100080

Smart RE, Bihgham GE (1974). Rapid estimates of relative water content. Plant Physiology 53:258-260. https://doi.org/10.1104/pp.53.2.258

Soliman M, Alhaithloul HA, Hakeem KR, Alharbi BM, El-Esawi M, Elkelish A (2020b). Exogenous nitric oxide mitigates nickel-induced oxidative damage in eggplant by up-regulating antioxidants, osmolyte metabolism, and glyoxalase systems. Plants 8:562. https://doi.org/10.3390/plants8120562

Soliman M, Elkelish A, Souad T, Alhaithloul H, Farooq M (2020a). Brassinosteroid seed priming with nitrogen supplementation improves salt tolerance in soybean. Physiology and Molecular Biology of Plants 26:501-511. https://doi.org/10.1007/s12298-020-00765-7

Stael S, Van Breusegem F, Gevaert K, Nowack MK (2019). Plant proteases and programmed cell death. Journal of Experimental Botany 70(7):1991-1995. https://doi.org/10.1093/jxb/erz126

Teerarak M, Charoenying P, Laosinwattana C (2012). Physiological and cellular mechanisms of natural herbicide resource from Aglaia odorata Lour. on bioassay plants. Acta Physiologiae Plantarum 34:1277-1285. https://doi.org/10.1007/s11738-011-0923-5

Tomar NS, Agarwal RM (2013). Influence of treatment of Jatropha curcas L. leachates and potassium on growth and phytochemical constituents of wheat (Triticum aestivum L.). American Journal of Plant Sciences 4(5):1134-1150. https://doi.org/10.4236/ajps.2013.45140

Tomar NS, Ahanger MA, Agarwal RM (2014). Jatropha curcas: An overview. In: Ahmad P, Wani MR (Eds). Physiological Mechanisms and Adaptation Strategies in Plants Under Changing Environment. Springer Science+ Business Media New York, pp 361-383. https://doi.org/10.1007/978-1-4614-8600-8_13

Tomar NS, Sharma M, Agarwal RM (2015). Phytochemical analysis of Jatropha curcas L. during different seasons and developmental stages and seedling growth of wheat Triticum aestivum L.) as affected by extracts/leachates of Jatropha curcas L. Physiology and Molecular Biology of Plants 21(1):83-92. https://doi.org/10.1007/s12298-014-0272-0

Turan S, Tripathy BC (2015) Salt-stress induced modulation of chlorophyll biosynthesis during deetiolation of rice seedlings. Physiologia Plantarum 153:477-491. https://doi.org/10.1111/ppl.12250

Tyagi, SR, Agarwal RM (2011). Analysis of Zizyphus mauritiana Lam. from allelopathic viewpoint. Journal of Functional and Experimental Botany 1(2):133-138.

Viswanath KK, Varakumar P, Pamuru RR, Basha SJ, Mehta S, Rao AD (2020). Plant lipoxygenases and their role in plant physiology. Journal of Plant Biology 63:83-95. https://doi.org/10.1007/s12374-020-09241-x

Wang JC, Wu Y, Wang Q, Peng YL, Par KW, Luo P, Wu N (2009). Allelopathic effects of Jatropha curcas on marigold (Tagetes erecta L.). Allelopathy Journal 24(1):123-130.

Wild R, Ooi L, Srikanth V, Münch G (2012) A quick, convenient and economical method for the reliable determination of methylglyoxal in millimolar concentrations: the N-acetyl-L-cysteine assay. Analytical and Bioanalytical Chemistry 403(9):2577-2581. https://doi.org/10.1007/s00216-012-6086-4

Yang QH, Ye WH, Liao FL, Yin XJ (2005). Effects of allelochemicals on seed germination. Chinese Journal of Ecology 24:1459-1465.

Zaid A, Wani SH (2019). Reactive oxygen species generation, scavenging and signaling in plant defense responses. In: Jogaiah S, Abdelrahman M (Eds). Bioactive Molecules in Plant Defense. Springer, Cham. https://doi.org/10.1007/978-3-030-27165-7_7

Zhang J, Mao Z, Wang L, Shu H (2007). Bioassay and identification of root exudates of three fruit tree species. Journal of Integrative Plant Biology 49:257-261. https://doi.org/10.1111/j.1744-7909.2007.00307.x

Zhang K-M, Shen Y, Zhou X-Q, Fang Y-M, Liu Y, Ma LQ (2016) Photosynthetic electron-transfer reactions in the gametophyte of Pteris multifida reveal the presence of allelopathic interference from the invasive plant species Bidens pilosa. Journal of Photochemistry and Photobiology B: Biology 158:81-88. https://doi.org/10.1016/j.jphotobiol.2016.02.026

Zhang S, Sun SW, Shi HL, Zhao K, Wang J, Liu Y, Liu XH, Wang W (2020). Physiological and biochemical mechanisms mediated by allelochemical isoliquiritigenin on the growth of lettuce seedlings. Plants 9(2):245. https://doi.org/10.3390/plants9020245

Zhang Y, Guan W, Tang MN, Li YH, Li L (2017). Effects of Casuarina equisetifoliaL. leachate on photosynthesis and antioxidant enzymes in seedlings of Hernandia nymphaeifolia (C. Presl) Kubitzki. Allelopathy Journal 40(2):209-224.

Published

2022-05-23

How to Cite

ALHAITHLOUL, H. A. S., & SOLIMAN, M. H. (2022). Responses of wheat and barley to Acacia saligna leaf and stem extracts: influence on growth and ascorbate-glutathione cycle. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(2), 12709. https://doi.org/10.15835/nbha50212709

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DOI: 10.15835/nbha50212709

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