Microscopical characteristics, phytochemical investigation and biological activities of different Lysimachia species growing in Vietnam

Quang Hung NGUYEN; Thanh Tung NGUYEN; Viet Than NGUYEN; Minh Ha LE; Ain RAAL

doi:10.15835/nbha50212692

Authors

Quang Hung NGUYEN Vietnam Academy of Science and Technology (VAST), Institute of Ecology and Biological Resources, Hoang Quoc Viet 18, Ha Noi; Graduate University of Science and Technology, VAST, Hoang Quoc Viet 18, Ha Noi (VN)
Thanh Tung NGUYEN Hanoi University of Pharmacy, Department of Pharmacognosy, Le Thanh Tong 15, Ha Noi (VN)
Viet Than NGUYEN Hanoi University of Pharmacy, Department of Pharmacognosy, Le Thanh Tong 15, Ha Noi (VN)
Minh Ha LE Vietnam Academy of Science and Technology (VAST), Institute of Natural Products Chemistry, Hoang Quoc Viet 18, Ha Noi (VN)
Ain RAAL University of Tartu, Institute of Pharmacy, Faculty of Medicine, Nooruse 1, Tartu 50411 (EE)

DOI:

https://doi.org/10.15835/nbha50212692

Keywords:

biological activity;, characteristics of stems and leaves, Lysimachia, metabolites, powder characteristics

Abstract

Several species of the genus Lysimachia L. (Primulaceae) are well known in folk medicine, but very few studies have been conducted on them. The aim of our study was to investigate the microscopical characteristics and chemical composition of Lysimachia insignis Hemsl., L. decurrens G. Forst., and L. congestiflora Hemsl. as well as to evaluate some biological activities of their methanol extracts. The transverse sections of stem and powder characteristics of the three Lysimachia species shared some similarities. Also, some differences were indicated. The chemical investigation of three aforesaid species including the preliminary phytochemical screening, the quantification of the total polyphenol content and total flavonoid content, as well as the determination of metabolites. The phytochemical screening showed the presence of flavonoids, saponins, tannins and sterols in all the three species. On the other hand, coumarins were detected in L. insignis while anthranoids were present in L. decurrens and L. congestiflora. The total polyphenol content and the total flavonoid content of the aforesaid species were determined by the spectrometric method. The metabolite profiling approach of three species was performed by LC-Q-ToF-HRMS and HPTLC. The methanol extract of L. insignis showed a weak inhibitory effect on NO production (I% = 57.4 at the concentration of 100 µg/ml) while the extract of L. decurrens exhibited moderate anti-oxidant activity in vitro (SC% = 62.5 at the concentration of 100 µg/mL).

Metrics

Metrics Loading ...

References

Brand-Williams W, Cuvelier M, Berset C (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology 28(1):25-30. https://doi.org/10.1016/S0023-6438(95)80008-5

Chinese Pharmacopoeia Commission (2020). 中华人民共和国药典 [Pharmacopoeia of the People's Republic of China]. Volume 1. China Medical Science Press, Beijing (in Chinese).

Dat NT, Binh PTX, Quynh LTP, Huong HT, Minh CV (2012). Sanggenon C and O inhibit NO production, iNOS expression and NF-κB activation in LPS-induced RAW264.7 cells. Immunopharmacology and Immunotoxicology 34(1):84-88. http://doi.org/10.3109/08923973.2011.580755

Germishuizen G, Meyer NL (eds.) (2003). Plants of Southern Africa an annotated checklist. Strelitzia 14:1-1231. National Botanical Institute, Pretoria.

Hu Q, Kelso S (1996). Lysimachia Linnaeus. In: Wu ZY, Raven PH (Eds). Flora of China. Science Press, Beijing & Missouri Botanical Garden Press, St. Louis, MO, pp 39-78.

Huang XA, Cai JZ., Hu YJ, Zhang YH (2007). Phytochemical investigation on Lysimachia fortunei. China Journal of Chinese Materia Medica 32(7):596-599 (in Chinese).

Liu YL, Tang LH, Liang ZQ, You BG, Yang SL (2010). Growth inhibitory and apoptosis inducing by effects of total flavonoids from Lysimachia clethroides Duby in human chronic myeloid leukemia K562 cells. Journal of Ethnopharmacology 131(1):1-9. http://doi.org/10.1016/j.jep.2010.04.008

Nguyen HT, Ho DV, Nguyen PDQ, Vo HQ, Do TT, Raal A. (2018). Cytotoxic evaluation of compunds isolated from the aerial parts of Hedyotis pilulifera and methanol extract of Inonotus obliquus. Natural Product Communications 13(8):939-941. http://doi.org/10.1177/1934578X1801300805

Ngo VT, Tran H (2011). Dược liệu học [Pharmacognosy]. Volume 1. Medical Publishing House, Hanoi (in Vietnamese).

Omar MY, Maroyi A, Van-Tol JJ (2016). Floral diversity, composition and distribution in a montane wetland in Hogsback, the Eastern Cape Province, South Africa. Parkistan Journal of Botany 48(5):1861-1870.

Pham TK, Nguyen TT (2015). Dược liệu học [Pharmacognosy]. Volume 2. Medical Publishing House, Hanoi (in Vietnamese).

Raal A, Nguyen TH, Ho VD, Do TT (2015). Selective cytotoxic action of Scots pine (Pinus sylvestris L.) needles extracts in human cancer cell lines. Pharmacognosy Magazine 11:290-295. http://doi.org/10.4103/0973-1296.166052

Seyfı P, Mostafaie A, Mansouri K, Arshadi D, Mohammadi-Motlagh HR, Kiani A (2010). In vitro and in vivo anti-angiogenesis effect of shallot (Allium ascalonicum): a heat-stable and flavonoid-rich fraction of shallot extract potently inhibits angiogenesis. Toxicology in Vitro 24(6):1655-1661. http://doi.org/10.1016/j.tiv.2010.05.022

Singleton VL, Orthofer R, Lamuela-Raventós RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology 299:152-178. https://doi.org/10.1016/S0076-6879(99)99017-1

Toth A, Toth G, Kery A (2014). Polyphenol composition and antioxidant capacity of three Lysimachia species. Natural Product Communications 9(10):1473-1478. https://doi.org/10.1177/1934578X1400901017

Toth A, Riethmuller E, Vegh K, Alberti A, Beni S, Kery A (2017). Contribution of individual flavonoid in Lysimachia species to the antioxidant capacity based on HPLC-DPPH assay. Natural Product Research 32(17):2058-2061. https://doi.org/10.1080/14786419.2017.1359176

Tran LTT, Ho DV, Le DV, Phan VK, Nguyen HT, Raal A (2019). Apoptosis-inducing effect of pogostemin A isolated from the aerial parts of Pogostemon auricularius against the human lung cancer cells. Journal of Biologically Active Products from Nature 9(5):320-327. http://doi.org/10.1080/22311866.2019.1687334

Vo VC (2012). Từ điển cây thuốc Việt Nam (bộ mới) [Dictionary of Vietnamese medicinal plants (new edition)], volume 2. Medical Publishing House, Hanoi pp 1064-1069. (in Vietnamese).

Wang DY, Liu EG, Feng YJ (2007). Study on the chemical constituents of Lysimachia congestiflora. Subtropical Plant Science 36(2):19-21 (in Chinese). http://www.yrdzwkx.com/EN/Y2007/V36/I02/19

Zhang X, Peng S, Xiao S, Wang M, Ding L (1998). Chemical constituents of Lysimachia candida. Chinese Journal of Applied and Environmental Biology 4(2): 145-147 (in Chinese). https://en.cnki.com.cn/Article_en/CJFDTotal-YYHS199802008.htm