Studies on the induction of basal stem cluster buds and nodes propagation of Amomum villosum Lour.
Keywords:Amomum villosum Lour, zingiberaceae, rhizomes, node propagation, tissue culture
Amomum villosum Lour. as Chinese herbal medicine and seasoning, which has a huge potential economic value. This plant is increasing shortage of resources by the slow sexual reproduction and the low artificial reproduction rate. The plants with strong stress resistance and high yield were selected as the female parent, on the basis of single factor and complete combination, the orthogonal test of L9 (34) and L16 (45) was further conducted. 6-BA induced basal stem cluster bud formation was obviously better than that of KT or ZT. 2, 4-D significantly induced callus occurrence and node enlargement, while NAA was more beneficial to adventitious root formation The combination of 6-BA and NAA was more beneficial to induce the formation of cluster buds from basal stem, but the proliferation effect was not ideal. Thus, KT or 2, 4-D was introduced for two orthogonal tests of L9 (34), and the highest proliferation coefficient was only 4.56. Then it was found that adding 0.5 mg·L-1 2, 4-D to the above combination, appeared a unique phenomenon of node propagation. Next, L16 (45) orthogonal test was conducted using 4 plant growth regulators combinations of 6-BA, 2, 4-D, KT and NAA. The optimal medium for proliferation culture was the MS medium with 7.5 mg·L-1 6-BA, 5.0 mg·L-1 NAA, 1.5 mg·L-1 KT, 0.5 mg·L-1 2, 4-D, and the proliferation coefficient reached above 10.00. The optimal rooting medium was the 1/2 MS medium with 2.0 mg·L-1 NAA. With the node propagation, a rapid propagation system of A. villosum was established which provided a possible solution for improve the efficiency of artificial planting, solve the market demand and quality problems.
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