Identification of the glycerol-3-phosphate dehydrogenase (GPDH) gene family in wheat and its expression profiling analysis under different stress treatments

Authors

  • Chao WANG Huazhong Agricultural University, College of Plant Science and Technology, Wuhan 430070 (CN)
  • Zixian ZHOU Jiangxi Agricultural University, College of Bioscience and Bioengineering, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045 (CN)
  • Shan JIANG Huazhong Agricultural University, College of Plant Science and Technology, Wuhan 430070 (CN)
  • Qiang LI Huazhong Agricultural University, College of Plant Science and Technology, Wuhan 430070 (CN)
  • Licao CUI Jiangxi Agricultural University, College of Bioscience and Bioengineering, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045 (CN)
  • Yong ZHOU Jiangxi Agricultural University, College of Bioscience and Bioengineering, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045 (CN)

DOI:

https://doi.org/10.15835/nbha50312611

Keywords:

abiotic stress, expression profile, glycerol-3-phosphate dehydrogenase (GPDH);, phylogeny, wheat

Abstract

Glycerol-3-phosphate dehydrogenase (GPDH) catalyses the interconversion of glycerol-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP), and plays key roles in different developmental processes and stress responses. GPDH family genes have been previously investigated in various plant species, such as Arabidopsis, maize, and soybean. However, very little is known in GPDH family genes in wheat. In this study, a total of 17 TaGPDH genes were identified from the wheat genome, including eight cytosolic GPDHs, six chloroplastic GPDHs and three mitochondrial GPDHs. Gene duplication analysis showed that segmental duplications contributed to the expansion of this gene family. Phylogenetic results showed that TaGPDHs were clustered into three groups with the same subcellular localization and domain distribution, and similar conserved motif arrangement and gene structure. Expression analysis based on the RNA-seq data showed that GPDH genes exhibited preferential expression in different tissues, and several genes displayed altered expression under various abiotic stresses. These findings provide the foundation for further research of wheat GPDH genes in plant growth, development and stress responses.

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Published

2022-09-06

How to Cite

WANG, C., ZHOU, Z., JIANG, S., LI, Q., CUI, L., & ZHOU, Y. (2022). Identification of the glycerol-3-phosphate dehydrogenase (GPDH) gene family in wheat and its expression profiling analysis under different stress treatments. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(3), 12611. https://doi.org/10.15835/nbha50312611

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Research Articles
CITATION
DOI: 10.15835/nbha50312611