Investigation of factors in improving Agrobacterium-mediated gene transfer in Ruellia tuberosa L. and evaluation of α-glucosidase inhibitory activity in established hairy roots

Authors

  • Dai M. CAO University of Sciences, Laboratory of Plant Biotechnology, Department of Plant Biotechnology and Biotransformation, Ho Chi Minh City; Vietnam National University, Ho Chi Minh City (VN)
  • Tram T.M. TRAN University of Sciences, Laboratory of Plant Biotechnology, Department of Plant Biotechnology and Biotransformation, Ho Chi Minh City; Vietnam National University, Ho Chi Minh City (VN)
  • Phuong N.D. QUACH University of Sciences, Laboratory of Plant Biotechnology, Department of Plant Biotechnology and Biotransformation, Ho Chi Minh City; Vietnam National University, Ho Chi Minh City (VN)

DOI:

https://doi.org/10.15835/nbha50312588

Keywords:

α-glucosidase, hairy root induction, induced factors, R. tuberosa, transfoemation rate

Abstract

Ruellia tuberosa (family Acanthaceae) is widely known in traditional medicine in Asian countries for the treatment of diabetes and other diseases. Its roots were demonstrated to possess a hypoglycemic ability in diabetic animal models. In this study, an original induced procedure was investigated to establish hairy root (HR) from R. tuberosa. With the aim of increasing the transformation rate, some induced factors (acetosyringone (AS) dosage, type of explant, age, infection time, bacterial density, co-cultivation duration) were individually examined. As a result, an improved procedure was implemented: ten-day-old in vitro cotyledon explants were injured and then immersed in the bacterial suspension (OD600 nm = 0.4) added 200 µM AS during 10 min. The infected explants were co-cultivated for 4 days in the Murashige & Skoog (MS) medium before transferring to the medium containing cefotaxime for bacterial elimination. After thirty days of culture, the improved procedure revealed a synergistic effect by enhancing the rooting rate and number of secondary roots per explant up to 4.4- and 8.0-fold, respectively, in comparison with the original procedure. The R. tuberosa HR was then cultured in liquid MS medium and achieved the highest biomass production at the late exponential growth phase (3rd week). Its ethanol extract was also higher 2.0-fold in α-glucosidase inhibitory activity than that of the natural root. In conclusion, the α-glucosidase inhibitory activity of HR inducing by the improved procedure may offer an effective and reliable substitute for the utilization of this herbal plant.

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References

Ali H, Houghton PJ, Soumyanath A (2006). α-amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology 107(3):449-455. https://doi.org/10.1016/j.jep.2006.04.004

Bergier K, Kuzniak E, Sklodowska M (2012). Antioxidant potential of Agrobacterium-transformed and non-transformed Physalis ixocarpa plants grown in vitro and ex vitro. Advances in Hygiene and Experimental Medicine 66:976-982. https://doi.org/10.5604/17322693.1023086

Bulgakov VP (2008). Functions of rol genes in plant secondary metabolism. Biotechnology Advances 26(4):318-324. https://doi.org/10.1016/j.biotechadv.2008.03.001

Cheruvathur MK, Jose B, Thomas TD (2015). Rhinacanthin production from hairy root cultures of Rhinacanthus nasutus (L.) Kurz, In Vitro Cellular & Development Biology-Plant 51:420-427. https://doi.org/10.1007/s11627-015-9694-9

Chilton MD, Tepfer DA, Petit A, David C, Casse Delbart F, Tempé J (1982). Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells. Nature 295:432-434. https://doi.org/10.1038/295432a0

Cujic N, Savikin K, Jankovic T, Pljevljakusic D, Zdunic G, Ibric S (2016). Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chemistry 194:135-142. https://doi.org/10.1016/j.foodchem.2015.08.008

Dong HQ, Li M, Zhu F, Huang JB (2012). Inhibitory potential of trilobatin from Lithocarpus polystachyus Rehd against α-glucosidase and α-amylase linked to type 2 diabetes. Food Chemistry 130(2):261-266. https://doi.org/10.1016/j.foodchem.2011.07.030

Duta FP, França FP, Lopes LMA (2006). Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method. Electronic Journal of Biotechnology 9(4). https://doi.org/10.2225/vol9-issue4-fulltext-7

Edwards K, Johnstone C, Thompson C (1991). A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research 19(6):1349. https://doi.org/10.1093/nar/19.6.1349

Geng L, Niu L, Gresshoff PM, Shu C, Song F, Huang D, Zhang J (2012). Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants in peanut (Arachis hypogaea L.). Plant Cell, Tissue and Organ Culture 109:491-500. https://doi.org/10.1007/s11240-012-0113-1

Georgiev MI, Pavlov AI, Bley T (2007). Hairy root type plant in vitro systems as sources of bioactive substances. Applied Microbiology and Biotechnology 74:1175-1185. https://doi.org/10.1007/s00253-007-0856-5

Grabkowska R, Królicka A, Mielicki W, Wielanek M, Wysokinska H (2010). Genetic transformation of Harpagophytum procumbens by Agrobacterium rhizogenes: iridoid and phenylethanoid glycoside accumulation in hairy root cultures. Acta Physiologiae Plantarum 32:665-673. https://doi.org/10.1007/s11738-009-0445-6

Guillon S, Trémouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006). Harnessing the potential of hairy roots: dawn of a new era. Trends in Biotechnology 24(9):403-409. https://doi.org/10.1016/j.tibtech.2006.07.002

Häkkinen ST, Oksman-Caldentey KM (2018). Progress and prospects of hairy root research. In: Hairy Roots: An Effective Tool of Plant Biotechnology. Springer, Singapore pp 3-19.

Hoque ME, Mansfield JW (2004). Effect of genotype and explant age on callus induction and subsequent plant regeneration from root-derived callus of Indica rice genotypes. Plant Cell, Tissue and Organ Culture 78:217-223. https://doi.org/10.1023/B:TICU.0000025640.75168.2d

Hu ZB, Alfermann AW (1993). Diterpenoid production in hairy root cultures of Salvia miltiorrhiza. Phytochemistry 32(3):699-703. https://doi.org/10.1016/S0031-9422(00)95156-2

Kang HJ, Anbazhagan VR, You XL, Moon HK, Yi JS, Choi YE (2006). Production of transgenic Aralia elata regenerated from Agrobacterium rhizogenes-mediated transformed roots. Plant Cell, Tissue and Organ Culture 85:187-196. https://doi.org/10.1007/s11240-005-9070-2

Kavitah G, Taghipour F, Huyop F (2010). Investigation of factors in optimizing Agrobacterium-mediated gene transfer in Citrullus lanatus cv. Round Dragon. Journal of Biological Sciences 10(3):209-216.

Kumar V, Jones B, Davey MR (1991). Transformation by Agrobacterium rhizogenes and regeneration of transgenic shoots of the wild soybean Glycine argyrea. Plant Cell Reports 10:135-138. https://doi.org/10.1007/BF00232044

Kurniawati ANL, Aulanni’am, Srihardyastutie A, Safitri A (2018). The effect of root extract Ruellia tuberosa L on histopathology and Malondialdehyde levels on the liver of diabetic rats. IOP Conference Series: Materials Science and Engineering 299(1):012022. https://doi.org/10.1088/1757-899X/299/1/012022

Mohan VR, Rajendra KN, Vasantha K (2014). GC-MS analysis of bioactive components of tubers of Ruellia tuberosa L. (Acanthaceae). American Journal of phytomedicine and clinical therapeutics 2(2):209-216.

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497.

Pawar PK, Maheshwari VL (2004). Agrobacterium rhizogenes mediated hairy root induction in two medicinally important members of family Solanaceae. Indian Journal of Biotechnology 3:414-417. http://nopr.niscair.res.in/handle/123456789/5875

Petrova M, Zayova E, Vlahova M (2013). Induction of hairy root in Arnica montana L. by Agrobacterium rhizogenes. Open Life Sciences 8(5):470-479. https://doi.org/10.2478/s11535-013-0157-6

Phan HT, Quach PND, Nguyen NN (2016). Hairy induction from Impatiens balsamina L. using fourteen Agrobacterium rhizogenes strains. Science and Technology Development Journal 19(2):38-47. https://doi.org/10.32508/stdj.v19i2.801

Phuong VTB, Hong PTA, Phuong QND (2018). Improving hairy root induction of Urena lobata L. by Agrobacterium rhizogenes ATCC 15834 by some factors. Science and Technology Development Journal, 21(3-4):90-97. https://doi.org/10.32508/stdj.v21i3.430

Rajan M, Kumar VK, Kumar PS, Swathi KR, Haritha S (2012). Antidiabetic, antihyperlipidaemic and hepatoprotective activity of methanolic extract of Ruellia tuberosa Linn leaves in normal and alloxan induced diabetic rats. Journal of Chemical and Pharmaceutical Research 4(6):2860-2868.

Roosdiana A, Mahdi C, Safitri A (2018, December). The influence of ethanolic root extracts of Ruellia tuberosa L. on pancreatic protease activity and MDA level of rats (Rattus norvegicus) induced by MLD-STZ. In: IOP Conference Series: Earth and Environmental Science 217(1):012041.

Safitri A, Roosdiana A, Evindasari CA (2019). Hypoglycaemic activity of hydroethanolic root extracts of Ruellia tuberosa L in diabetic rats. In: Journal of Physics: Conference Series 1146(1):012020.

Saleh NM, Thuc LV (2009). Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes. African Journal of Biotechnology 8(15):3519-3523.

Shahwar D, Ullaha S, Ahmad M, Ullah S, Ahmad N, Khan MA (2011). Hypoglycemic Activity of Ruellia tuberosa Linn (Acanthaceae) in Normal and Alloxan-Induced Diabetic Rabbits. Iranian Journal of Pharmaceutical Sciences 7(2):107-115.

Shibuya M, Xiang T, Katsube Y, Otsuka M, Zhang H, Yutaka EY (2007). Origin of structural diversity in natural triterpenes: direct synthesis of seco-triterpene skeletons by oxidosqualene cyclase. Journal of the American Chemical Society 129(5):1450-1455. https://doi.org/10.1021/ja066873w

Sinkar VP, White FF, Gordon MP (1987). Molecular biology of Ri-plasmid—a review. Journal of Biosciences, 11(1):47-57. https://doi.org/10.1007/BF02704657

Su J, Duan RQ, Hu CQ, Li YP, Wang F (2002). Regeneration and Agrobacterium-mediated transformation for Chinese cabbage. Fujian Journal of Agriculture Science 17(4):241-243.

Tusevski O, Stanoeva JP, Stefova M, Spasenoski M, Simic SG (2019). State of antioxidant systems and phenolic compounds’ production in Hypericum perforatum L. hairy roots. Acta Physiologiae Plantarum 41:132. https://doi.org/10.1007/s11738-019-2919-5

Veerasham C (2004). Medicinal plant biotechnology. CBS, New Delhi, pp 377-419.

William S, Feil H, Copeland A (2012). Bacterial genomic DNA isolation using CTAB. Sigma 50(6876).

Wulan DR, Utomo EP, Mahdi C (2015). Antidiabetic Activity of Ruellia tuberosa L., Role of α-amylase Inhibitor: In Silico, In Vitro, and In Vivo Approaches. Biochemistry Research International 1-9. https://doi.org/10.1155/2015/349261

Published

2022-09-15

How to Cite

CAO, D. M., TRAN, T. T., & QUACH, P. N. (2022). Investigation of factors in improving Agrobacterium-mediated gene transfer in Ruellia tuberosa L. and evaluation of α-glucosidase inhibitory activity in established hairy roots. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(3), 12588. https://doi.org/10.15835/nbha50312588

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Research Articles
CITATION
DOI: 10.15835/nbha50312588