Effects of temperatures on growth, physiological, and antioxidant characteristics in Houttuynia cordata
Keywords:flavonoids, medicinal plant, phenolic compounds, spectral reflectance, temperature stress
Houttuynia cordata Thunb. (HC) is a traditional medicinal plant with a variety of pharmaceutical activities. The objective of this study was to investigate the growth, photosynthetic parameters, and antioxidant properties of HC plants in response to various temperatures. Pots of HC plants were maintained in day/night temperatures of 15/10 °C, 20/15 °C, 25/20 °C (control), 30/25 °C, and 35/30 °C for two months in each of five growth chambers having a 13.5 h photoperiod at 396, 432, 474, 449, and 619 µmol·m-2·s-1 radiation, respectively. Eight plants for each temperature were randomly placed in a growth chamber. HC plants survived at 30/25 °C and 35/30 °C treatments and had significantly higher plant heights, leaf numbers, and soil-plant analysis development (SPAD) and normalized difference vegetation index (NDVI) values compared to other treatments. However, long-term 35/30 °C treatment caused reductions in leaf length and width, significantly decreasing shoot and leaf fresh weight (FW) and dry weight (DW) compared to 30/25 °C treatment and controls. These results indicate that HC leaf development was affected during the 35/30 °C treatment, and that both SPAD and NDVI can help in advancing our understanding of the photosynthesis process in HC. Moreover, all plants subjected to 15/10 °C suffered more severely in all traits and parameters than other treatments. Therefore, HC plants tended to be heat-tolerant and exhibited adaptive morphologic plasticity to 30/25 °C conditions. Positive and significant correlations were observed among temperatures and total phenolics (TP), total flavonoids (TF), chlorogenic acid (CGA), and hyperoside (HO) content, and all bioactive contents increased as temperature increased, except that both CGA and HO content were remarkably decreased after 30/25 °C treatment. Thus, 30/25 °C treatment would be more beneficial for high marketability resulting from increased leaf number, DW, and all secondary metabolites compared to other treatments, and for use as a health food and for medicinal purposes. In addition, leaf growth, physiological parameters, and secondary metabolite accumulations in HC plants can be optimized for commercial production via temperature control technologies. This approach may also be applicable to leafy vegetables to produce stable industrial supplies having high leaf yields and metabolite content.
Ambrosio ND, Arena C, De Santo AV (2006). Temperature response of photosynthesis, excitation energy dissipation and alternative electron sinks to carbon assimilation in Beta vulgaris L. Environmental and Experimental Botany 55:248-257. https://doi.org/10.1016/j.envexpbot.2004.11.006
Ballester C, Zarco-Tejada PJ, Nicola E, Alarco JJ, Fereres E, Intrigliolo D, Gonzalez-Dugo V (2018). Evaluating the performance of xanthophyll, chlorophyll and structure-sensitive spectral indices to detect water stress in five fruit tree species. Precision Agriculture 19:178-193. https://doi.org/10.1007/s11119-017-9512-y
Chou SC, Su CR, Ku YC, Wu TS (2009). The constituents and their bioactivities of Houttuynia cordata. Chemical and Pharmaceutical Bulletin 57:1227-1230.
Chaves I, Passarinho JAP, Capitao C, Chaves MM, Fevereiro P, Ricardo CPP (2011). Temperature stress effects in Quercus suber leaf metabolism. Journal of Plant Physiology 168:1729-1734. https://doi.org/10.1016/j.jplph.2011.05.013
Chung IM, Kim JJ, Lim JD, Yu C, Kim SH, Hahn SJ (2006). Comparison of resveratrol, SOD activity, phenolic compounds and free amino acids in Rehmannia glutinosa under temperature and water stress. Environmental and Experimental Botany 56:44-53. https://doi.org/10.1016/j.envexpbot.2005.01.001
Fu JG, Dai L, Lin Z, Lu HM (2013). Houttuynia cordata Thunb: a review of phytochemistry and pharmacology and quality control. Chinese Medicine 4:101-123. https://doi.org/10.4236/cm.2013.43015
Goh HH, Khairudin N, Sukiran N, Normah M, Baharum S (2016). Metabolite profiling reveals temperature effects on the VOCs and flavonoids of different plant populations. Plant Biology 18:130-139. https://doi.org/10.1111/plb.12403
Hern´andez-Clemente R, Navarro-Cerrillo R, Morales F, Zarco-Tejada PJ (2011). Assessing structural effects on PRI for stress detection in conifer forests. Remote Sensing of Environment 115:2360-2375. https://doi.org/10.1016/j.rse.2011.04.036
Habibi G (2018). Effects of mild and severe drought stress on the biomass, phenolic compounds production and photochemical activity of Aloe vera (L.) Burm.f. Acta Agriculturae Slovenica 111:463-476.
Hsu CC, Yang HT, Ho JJ (2016). Houttuynia cordata aqueous extract attenuated glycative and oxidative stress in heart and kidney of diabetic mice. European Journal of Nutrition 55(2):845-854. https://doi.org/10.1007/s00394-015-0994-y
Jiang N, Dose F, Grotewold E (2016). Flavones: From biosynthesis to health benefits. Plants 5:27. https://doi.org/10.3390/plants5020027
Kreslavski VD, Lyubimov VY, Shabnova NI, Balakhnina TI, Kosobryukhov AA (2009). Heat-induced impairments and recovery of photosynthetic machinery in wheat seedlings. Role of light and prooxidant-antioxidant balance. Physiology and Molecular Biology of Plant 15:115-122
Kim HS (2010). A review of medical effects and industrialization of Houttuynia cordate. Journal of Oriental Academia 3:145-149.
Kim GS, Kim DH, Lim JJ, Lee JJ, Han DY, Lee WM, … Kim S (2008). Biological and antibacterial activities of the natural herb Houttuynia cordata water extract against the intracellular bacterial pathogen salmonella within the RAW 264.7 macrophage. Biological and Pharmaceutical Bulletin 31:2012-2017. https://doi.org/10.1248/bpb.31.2012
Kumar M, Prasad SK, Hemalatha S (2014). A current update on the phytopharmacological aspects of Houttuynia cordata Thunb. Pharmacognosy Review 8(15):22-35. https://doi.org/10.4103/0973-7847.125525
Lin KH, Jhou YH, Wu CW, Chang YS (2020). Growth, physiological, and antioxidant characteristics in green and red Perilla frutescens varieties as affected by temperature- and water-stressed conditions. Scientia Horticulturae 274:109682. https://doi.org/10.1016/j.scienta.2020.109682
Li W, Zhou P, Zhang Y, He L (2011). Houttuynia cordata, a novel and selective COX-2 inhibitor with anti-inflammatory activity. Journal of Ethnopharmacology 133(2):922-927. https://doi.org/10.1016/j.jep.2010.10.048
Lou Y, Guo Z, Zhu Y, Kong M, Zhang R, Lu L, Wu F, Liu Z, Wu J (2019). Houttuynia cordata thunb. and its bioactive compound 2-undecanone significantly suppress benzo(a)pyrene-induced lung tumorigenesis by activating the Nrf2-HO-1/ NQO-1 signaling pathway. Journal of Experimental and Clinical Cancer Research 38:242. https://doi.org/10.1186/s13046-019-1255-3
Lu H, Liang Y, Chen S: (2006). Identification and quality assessment of Houttuynia cordata injection using GC–MS fingerprint: A standardization approach. Journal of Ethnopharmacology 105:436-440. https://doi.org/10.1016/j.jep.2005.11.018
Lu N, Takagaki M, Yamori W, Kagawa N (2018). Flavonoid productivity optimized for green and red forms of Perilla frutescens via environmental control technologies in plant factory. Journal of Food Quality 4270279. https://doi.org/10.1155/2018/4270279
Meng J, Leung KSY, Dong XP, Zhou YS, Jiang ZH, Zhao ZZ (2009). Simultaneous quantification of eight bioactive components of Houttuynia cordata and related Saururaceae medicinal plants by on-line high-performance liquid chromatography-diode array detector-electrospray mass spectrometry. Fitoterapia 80:468-474. https://doi.org/10.1016/j.fitote.2009.06.013
Nguyen VT, Le VM, Vo TS, Bui LM, Anh HLT, Danh VT (2020). Preliminary phytochemical screening and determination of total polyphenols and flavonoids content in the leaves of Houttuynia cordata Thunb. In: IOP Conference Series: Materials Science and Engineering 736:062013. https://doi.org/10.1088/1757-899X/736/6/062013
Ni YW, Lin KH, Chen HK, Wu CW, Chang YS (2020). Flavonoid compounds and photosynthesis in Passiflora plant leaves under varying light intensities. Plants 9:633. https://doi.org/10.3390/plants9050633
Nuengchamnong N, Krittasilp K, Ingkaninan K (2009) Rapid screening and identification of antioxidants in aqueous extracts of Houttuynia cordata using LC–ESI–MS coupled with DPPH assay. Food Chemistry 117(4):750-756. https://doi.org/10.1016/j.foodchem.2009.04.071
Peñuelas J, Filella I, Gamon J (1995). Assessment of photosynthetic radiation use efficiency with spectral reflectance. New Phytologist 131:291-296. https://doi.org/10.1111/j.1469-8137.1995.tb03064.x
Porcar-Castell A, Pfündel E, Korhonen FJ, Juurola E (2008). A new monitoring PAM fluorometer (Mini-Pam) to study the short- and long-term acclimation of photosystem II in field conditions. Photosynthesis Research 96:173-179. https://doi.org/10.1007/s11120-008-9292-3
Pourmorad F, Hosseinimehr SJ, Shahabimajd N (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology 5:1142-1145.
Rouphael Y, Kyriacou MC, Petropoulos SA, de Pascale S, Colla G (2018). Improving vegetable quality in controlled environments. Scientia Horticulturae 234:275-289. https://doi.org/10.1016/j.scienta.2018.02.033
Rahbarian R, Khavari-Nejad R, Ganjeali A, Bagheri A, Najafi F (2011). Drought stress effects on photosynthesis, chlorophyll fluorescence and water relations in tolerant and susceptible chickpea (Cicer Arietinum L.) genotypes. Acta Biologica Cracoviensia s. Botanica 53:47-56. https://doi.org/10.2478/v10182-011-0007-2
Saravanan S, Parimelazhagan T (2013). Total phenolic content, free radical scavenging and antimicrobial activities of Passiflora subpeltata seeds. Journal of Applied Pharmaceutical Science 4:67-72. https://doi.org/10.7324/JAPS.2013.3412
Shingnaisui K, Dey T, Manna P, Kalita J (2018). Therapeutic potentials of Houttuynia cordata Thunb. against inflammation and oxidative stress: A review. Journal of Ethnopharmacology 220:35-43. https://doi.org/10.1016/j.jep.2018.03.038
Tian L, Zhao Y, Guo C, Yang X (2011). A comparative study on the antioxidant activities of an acidic polysaccharide and various solvent extracts derived from herbal Houttuynia cordata. Carbohydrate Polymers 83(2):537-544. https://doi.org/10.1016/j.carbpol.2010.08.023
Tian L, Shi X, Yu L, Zhu J, Ma R, Yang X (2012). Chemical composition and hepatoprotective effects of polyphenol-rich extract from Houttuynia cordata tea. Journal of Agricultural and Food Chemistry 60:4641-4648. https://doi.org/dx.doi.org/10.1021/jf3008376
Wang SY, Bunce JA, Maas JL (2013). Elevated carbon dioxide increases contents of antioxidant compounds in field-grown strawberries. Journal of Agricultural and Food Chemistry 51:4315-4320. https://doi.org/10.1021/jf021172d
Wang SY Zheng W (2001). Effect of plant growth temperature on antioxidant capacity in strawberry. Journal of Agricultural and Food Chemistry 49:4977-4982.
Wang XP, Ye MR, Zhang XP, Zhang LF, Jing NN, Su W (2016). Physiological adaptation of Houttuynia cordata to substrate moisture change under shading condition. Wetland Science 14(3):446-450.
Wahid A (2007) Physiological implications of metabolite biosynthesis for net assimilation and heat-stress tolerance of sugarcane (Saccharum officinarum) sprouts. Journal of Plant Research 120:219-228. https://doi.org/10.1007/s10265-006-0040-5
Weng JH, Jhaung LH, Lin RJ, Chen HY (2010). Relationship between photochemical efficiency of photosystem II and the photochemical reflectance index of mango tree: merging data from different illuminations, seasons and leaf colors. Tree Physiology 30:469-478. https://doi.org/10.1093/treephys/tpq007
Wu Z, Deng X, Hu Q, Xiao X, Jiang J, Ma X, Wu M (2021). Houttuynia cordata Thunb: An Ethnopharmacological Review. Frontiers in Pharmacology 12:714694. https://doi.org/10.3389/fphar.2021.714694
Yoon SR, Shim SM (2015). Inhibitory effect of polyphenol in Houttuynia cordata on advanced glycation end-products (AGEs) by trapping methylglyoxal. LWT - Food Science and Technology 61(1):158-163. https://doi.org/10.1016/j.lwt.2014.11.014
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