Effects of EDTA and aqueous plants extract on the developmental and stress tolerance attributes of Spinacia oleracea and Brassica rapa under sewage water regime


  • Ghalia S. H. ALNUSAIRI Jouf University, Department of Biology, College of Science (SA)
  • Mona H. SOLIMAN Taibah University, Faculty of Science, Biology Department (SA)
  • Amir Abdullah KHAN Department of Plant Biology and Ecology, Nankai University, Tianjin 30007, China (CN)
  • Awatif M. ABDULMAJEED University of Tabuk, Faculty of Science, Biology Department (SA)
  • Ayshah A. ALRASHIDI University of Hail, Department of Biology, Faculty of Science (SA)
  • Mohamed S. ATTIA Al-Azhar University, Botany and Microbiology Department, Faculty of Science (EG)
  • Haitham S. MADY Egyptian Environmental Affairs Agency (E.E.A.A), Cairo, Egypt (EG)
  • Emad A. EWAIS Al-Azhar University, Botany and Microbiology Department, Faculty of Science (EG)
  • Md. MAHADI HASAN Lanzhou University, State Key Laboratory of Grassland Agro-Ecosystems, School of Life Sciences (CN)
  • Shehzad MEHMOOD Quaid-i-Azam University, Faculty of Biological Sciences (PK)
  • Mohamed M. HASSAN Taif University, Department of Biology, College of Science (SA)
  • Jamal A. ALORABI Taif University, Department of Biology, College of Science (SA)
  • Hoda H. SENOUSY Cairo University, Faculty of Science, Botany and Microbiology Department (EG)
  • Taghreed S. Alnusaire (SA)




antioxidants, biochemical constituents, heavy metals, proline, SDS-PAGE


Sewage water is causing a potential threat to agriculture sector due to industrial effluents having heavy metals. Present investigation was carried to study the role of ethylenediaminetetraacetic acid (EDTA) or aqueous extracts of Hyacinth and Hedychium on soil quality and growth of spinach and turnip plants irrigated with sewage water (SW). Treatment of plants with SW resulted in an increment of catalase (CAT), peroxidase (POD) and polyphenol oxidase (PPO) activities. However, EDTA or plant extracts further enhanced their activities. At both stages of development of the tested crops, a substantial increase was found in the content of proline and total phenols, indicating the strengthening of the antioxidant protection mechanism to boost the oxidative effects of SW stress. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) studies revealed considerable variation in the protein profile among the different treatments, with an expression of some unique proteins obvious with other treatments. SW treatments increased heavy metals (HM) content in soil and plants; however, EDTA or plant extracts greatly decreased the levels of HMs in both shoots and roots and soils. The present study results suggest that the application of EDTA or aqueous plant extracts can be a useful strategy for phytoextraction in areas irrigated with sewage water.


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How to Cite

S. H. ALNUSAIRI, G. ., H. SOLIMAN, M. ., KHAN, A. A., M. ABDULMAJEED, A. ., A. ALRASHIDI, A. ., S. ATTIA, M. ., S. MADY, H. ., A. EWAIS, E. ., MAHADI HASAN, M., MEHMOOD, S., HASSAN, M. M., ALORABI, J. A., H. SENOUSY, H. ., & S. Alnusaire, T. . (2022). Effects of EDTA and aqueous plants extract on the developmental and stress tolerance attributes of Spinacia oleracea and Brassica rapa under sewage water regime. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(1), 12534. https://doi.org/10.15835/nbha50112534



Research Articles
DOI: 10.15835/nbha50112534