Genome-wide investigation of Hydroxycinnamoyl CoA: Shikimate Hydroxycinnamoyl Transferase (HCT) gene family in Carthamus tinctorius L.

  • Sun FAN Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118 (CN)
  • Naveed AHMAD Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118; Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Shandong Provincial Key, Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100 (CN)
  • Jin LIBO Wenzhou University, Institute of Life Sciences, Wenzhou 325035;Wenzhou University, Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou, Zhejiang 325035 (CN)
  • Zhang XINYUE Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118 (CN)
  • Ma XINTONG Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118 (CN)
  • Nguyen Q. V. HOANG Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118 (CN)
  • Ali I. MALLANO Shandong Academy of Agricultural Sciences, Biotechnology Research Center, Shandong Provincial Key, Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100 (CN)
  • Wang NAN Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118 (CN)
  • Yang ZHUODA Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118 (CN)
  • Liu XIUMING Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118, (CN)
  • Yao NA Jilin Agricultural University, Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Changchun 130118 (CN)
Keywords: abiotic stress, Carthamus tinctorius, expression diversity, HCT gene family, monolignol biosynthesis

Abstract

Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) is mainly associated with monolignol biosynthesis, a central precursor to producing guaiacyl and syringyl lignins in plants. However, the explicit regulatory mechanism of HCT-mediated monolignol biosynthesis in plants still remained unclear. Here, the genome-wide analysis of the HCT gene family in Carthamus tinctorius as a target for understanding growth, development, and stress-responsive mechanisms was investigated. A total of 82 CtHCT genes were identified and characterized. Most of the CtHCTs proteins demonstrated the presence of two common conserved domains, including HXXXD and DFGWG. In addition, the conserved structure of protein motifs, PPI network, cis-regulatory units, and gene structure analysis demonstrated several genetic determinants reflecting the wide range of functional diversity of CtHCT-encoding genes. The observed expression analysis of CtHCT genes in different flowering stages under normal conditions partially highlighted their putative roles in plant growth and development pathways. Moreover, CtHCT genes appeared to be associated with abiotic stress responses as validated by the expression profiling in various flowering phases under light irradiation and MeJA treatment. Altogether, these findings provide new insights into identifying crucial molecular targets associated with plant growth and development and present practical information for understanding abiotic stress-responsive mechanisms in plants.

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Published
2021-09-27
How to Cite
FAN, S., AHMAD, N., LIBO, J., XINYUE, Z., XINTONG, M., HOANG, N. Q. V., MALLANO, A. I., NAN, W., ZHUODA, Y., XIUMING, L., & NA, Y. (2021). Genome-wide investigation of Hydroxycinnamoyl CoA: Shikimate Hydroxycinnamoyl Transferase (HCT) gene family in Carthamus tinctorius L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(3), 12489. https://doi.org/10.15835/nbha49312489
Section
Research Articles
CITATION
DOI: 10.15835/nbha49312489