Amino acids, bioactive compounds and biological activities of ten species from family Commelinaceae in Thailand

  • Theeraphan CHUMROENPHAT Mahasarakham University, Laboratory Equipment Center, Division of Research facilitation and dissemination, Kantarawichai District, Maha Sarakham, 44150 (TH)
  • Surapon SAENSOUK Mahasarakham University, Plant and Invertebrate Taxonomy and Its Applications Unit Group, Walai Rukhavej Botanical Research Institute, Kantarawichai District, Maha Sarakham, 44150 (TH)
Keywords: amino acids, antioxidants, Commelinaceae, flavonoids, phenolic acids


Commelinaceae is used for ethnobotany. However, bioactive compounds information of this family is few data. The aim here was to determine the bioactive compounds and quantify the antioxidant activity from leaves 10 species.  Twenty amino acids were identified using LC/MS/MS. The antioxidant contents were evaluated by the total phenolic and total flavonoid content assays. The individual phenolic acids, flavonoids and vitamin c were identified by HPLC. The antioxidant activities detected were DPPH scavenging and FRAP assay. The total amino acids found in most samples studied ranging from 239 to 1012 µg/g DW. Vitamin C contents were in the range of 23 to 195 mg/100 g DW. Total phenolic and total flavonoid contents ranged from 11.3 to 35.7 mg GAE/g DW and 56.7 to 368.7 mg RE/100 g DW, respectively. All the species studied possessed strong antioxidant properties (DPPH and FRAP). This result can be applied for further development of functional foods or cosmetics.


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Al-Duais M, Müller L, Böhm V, Jetschke G (2009). Antioxidant capacity and total phenolics of Cyphostemma digitatum before and after processing: use of different assays. European Food research and Technology 228:813-821.

Alonso-Castro AJ, Villarreal ML, Salazar-Olivo LA, Gomez-Sanchez M, Dominguez F, Garcia-Carranca A (2011). Mexican medicinal plants used for cancer treatment: pharmacological, phytochemical and ethnobotanical studies. Journal of Ethnopharmacology 133:945-972.

Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry 239:70-76.

Butsat S, Weerapreeyakul N, Siriamornpun S (2009). Changes in phenolic acids and antioxidant activity in Thai rice husk at five growth stages during grain development. Journal of Agricultural and Food Chemistry 57:4566-4571.

Cheeptham N, Towers GHN (2002). Light-mediated activities of some Thai medicinal plant teas. Fitoterapia 73(7-8):651-662.

Chumroenphat T, Somboonwatthanakul I, Saensouk S, Siriamornpun S (2019). The diversity of biologically active compounds in the rhizomes of recently discovered Zingiberaceae plants native to North Eastern Thailand. Pharmacognosy Journal 11.

Chumroenphat T, Somboonwatthanakul I, Saensouk S, Siriamornpun S (2021). Changes in curcuminoids and chemical components of turmeric (Curcuma longa L.) under freeze-drying and low-temperature drying methods. Food Chemistry 339.

Edeoga HO, Ogbebor NO (1999). Distribution of calcium oxalate crystals in some Nigerian species of Aneilema R. Br. (Commelinaceae). Plant Biosystem 133:193-198.

Ghasemzadeh A, Jaafar H Z, Rahmat A (2010). Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules 15:4324-4333.

González-Avila M, Arriaga-Alba M, De la Garza M, del Carmen Hernández Pretelı́n M, Domınguez-Ortız MA, Fattel-Fazenda S, Villa-Trevino S (2003). Antigenotoxic, antimutagenic and ROS scavenging activities of a Rhoeo discolor ethanolic crude extract. Toxicology in Vitro 17:77-83.

Huang M, Zhang H, Zhao C, Chen G, Zou Y (2019). Amino acid content in rice grains is affected by high temperature during the early grain-filling period. Scientific Reports 9:1-7.

Kaisoon O, Konczak I, Siriamornpun S (2012). Potential health enhancing properties of edible flowers from Thailand. Food Research International 46:563-571.

Kamble S (2019). Nutraceutical investigations of Commelina diffusa Burm. f. leaves-a popular wild vegetable. Plantae Scientia 2:34-39.

Khalid II, Elhardallou SB, Gobouri AA (2016). Amino acid composition and physicochemical properties of bitter lupine (Lupinustermis) seed flour. Oriental Journal of Chemistry 32(6):3175.

Kokilavani P, Suriyakalaa U, Elumalai P, Abirami B, Ramachandran, R, Sankarganesh A, Achiraman S (2014). Antioxidant mediated ameliorative steroidogenesis by Commelina benghalensis L. and Cissus quadrangularis L. against quinalphos induced male reproductive toxicity. Pesticide Biochemistry and Physiology 109:18-33.

Kubola J, Siriamornpun S, Meeso N (2011). Phytochemicals, vitamin C and sugar content of Thai wild fruits. Food Chemistry 126:972-981.

Kunnaja P, Wongpalee SP, Panthong A (2014). Evaluation of anti-inflammatory, analgesic, and antipyretic activities of the ethanol extract from Murdannia loriformis (Hassk.) Rolla Rao et Kammathy. BioImpacts BI4:183.

Li D, Li B, Ma Y, Sun X, Lin Y, Meng X (2017). Polyphenols, anthocyanins, and flavonoids contents and the antioxidant capacity of various cultivars of highbush and half-high blueberries. Journal of Food Composition and Analysis 62:84-93.

Mensah AY, Houghton PJ, Dickson RA, Fleischer TC, Heinrich M, Bremner P (2006). In vitro evaluation of effects of two Ghanaian plants relevant to wound healing. Phytotherapy Research 20:941-944.

Myriam AA, Luis BJ, Jannete R P N, Jaime SN, Roberto RS, Marisela GA (2011). Antimutagenicity mechanisms of the Rhoeo discolor ethanolic extract. Experimental and Toxicologic Pathology 63:243-248.

Ratseewo J, Meeso N, Siriamornpun S (2020). Changes in amino acids and bioactive compounds of pigmented rice as affected by far-infrared radiation and hot air drying. Food Chemistry 306.

Regis AS, Gabriel JJ (2017). Antifungal activity of Cyanotis axillaris (l.) d. DON EX sweet against opportunistic fungal strains. International Journal of Pharmacy and Pharmaceutical Sciences 9(7):140-143.

Ribeiro B, Andrade PB, Silva BM, Baptista P, Seabra RM, Valentao P (2008). Comparative study on free amino acid composition of wild edible mushroom species. Journal of Agricultural and Food Chemistry 56:10973-10979.

Rivero-Pérez MD, MUNiz PILAR., González-Sanjosé ML (2007). Antioxidant profile of red wines evaluated by total antioxidant capacity, scavenger activity, and biomarkers of oxidative stress methodologies. Journal of Agricultural and Food Chemistry 55:5476-5483.

Sánchez‐Moreno C, Larrauri JA, Saura‐Calixto F (1998). A procedure to measure the antiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture 76:270-276.;2-9

Siriamornpun S, Kaewseejan N (2017). Quality, bioactive compounds and antioxidant capacity of selected climacteric fruits with relation to their maturity. Scientia Horticulturae 221:33-42.

Tan JBL, Yap W J, Tan SY, Lim YY, Lee SM (2014). Antioxidant content, antioxidant activity, and antibacterial activity of five plants from the Commelinaceae family. Antioxidants 3:758-769.

Tan, JBL, Kwan YM (2020). The biological activities of the spiderworts (Tradescantia). Food Chemistry 317.

Wilson AK (1981). Commelinaceae-A review of the distribution, biology and control of the important weeds belonging to this family. International Journal of Pest Management 27:405-418.

Zhishen J, Mengcheng T, Jianming W (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64:555-559.

How to Cite
CHUMROENPHAT, T., & SAENSOUK, S. (2021). Amino acids, bioactive compounds and biological activities of ten species from family Commelinaceae in Thailand. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(3), 12391.
Research Articles
DOI: 10.15835/nbha49312391