Comparison of some DNA extraction methods from monovarietal must and wines

  • Anca P. ONACHE National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
  • Adriana BĂDULESCU National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
  • Anamaria M. DUMITRU National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
  • Dorin I. SUMEDREA National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
  • Carmen F. POPESCU National Research and Development Institute for Biotechnology in Horticulture, Ștefăneşti, Calea Bucureşti, Piteşti, 37, Ștefăneşti, Argeş (RO)
Keywords: DNA quality, grapevine, spectrophotometry, SSR markers, traceability

Abstract

The methods applied for DNA extraction from must and wine samples with monovarietal origin are presented and discussed aiming to prove the quality of extracted DNA and its good properties for further use in molecular tests. In the present research were compared four different DNA extraction methods from must and wine samples obtained from eleven V. vinifera varieties (five grapevine varieties for white wines and six grapevine varieties for red wines, respectively). Taking into consideration the amounts of obtained DNA, the concentrations and purities of the final DNA extracts, were stood out two modified methods. For all must samples, very efficient was the second method, which allowed obtaining a mean value of 87.9 ng µl-1 for the DNA concentration with 1.55 purity. Among the tested procedures, for monovarietal wine samples, the fourth method proved to be the most efficient which brought a mean value of 64.7 ng μl-1 for DNA concentration with 1.66 purity. This method adequate for wine samples involves two CTAB solution treatments and the RNase treatment applied before DNA resuspension. The DNA from must and wine extracts and the DNA from leaves of the corresponding grapevine varieties were amplified with five specific microsatellite primers (VVS2, VVMD27, VVMD32, VrZAG79 and VrZAG62). The aspects of pattern profiles were analysed in parallel and proved that the extracted DNA was suitable for amplification with these specific V. vinifera primers. The two selected extraction procedures are considered good for research purposes and ensure obtaining of good-quality extracted DNA from musts and one-year old wines.

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Published
2021-06-04
How to Cite
ONACHE, A. P., BĂDULESCU, A., DUMITRU, A. M., SUMEDREA, D. I., & POPESCU, C. F. (2021). Comparison of some DNA extraction methods from monovarietal must and wines. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12349. https://doi.org/10.15835/nbha49212349
Section
Research Articles
CITATION
DOI: 10.15835/nbha49212349