Nitrogen and potassium supplied by phenological stages affect the carotenoid and nutritive content of the tomato fruit


  • Cesar SAN MARTÍN-HERNÁNDEZ College of Postgraduates in Agricultural Sciences Campus Montecillo. Montecillo, State of Mexico (MX)
  • Fernando C. GÓMEZ-MERINO College of Postgraduates in Agricultural Sciences Campus Montecillo. Montecillo, State of Mexico (MX)
  • Crescenciano SAUCEDO-VELOZ College of Postgraduates in Agricultural Sciences Campus Montecillo. Montecillo, State of Mexico (MX)
  • Eber A. QUINTANA-OBREGÓN CONACYT-Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación Tecnología de Alimentos de Origen Vegetal. Hermosillo, Sonora (MX)
  • María D. MUY-RANGEL Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación Culiacán. Culiacán de Rosales, Sinaloa (MX)
  • Libia I. TREJO-TÉLLEZ College of Postgraduates in Agricultural Sciences Campus Montecillo. Montecillo, State of Mexico (MX)



β-carotene, lycopene, protein, Solanum lycopersicum L., sugars, vitamin C


The effect of nitrogen (N) and potassium (K) supply by phenological stages of horticultural crops such as tomato has been little explored so far. In this study, we evaluated the impact of N supply in the vegetative stage and K in the reproductive stage of tomato, on the carotenoid and nutritive content of fruits of three truss clusters. The concentrations of protein, lycopene, β-carotene, sugars, vitamin C and fruit juice were affected by the N and K application by phenological stages, although the N×K interaction was not significant in the last three variables. Increases in N from 10 to 16 molc m-3 of nutrient solution (NS) in the vegetative stage of the crop increased the concentrations of protein, vitamin C, sugars (temporarily) and fruit juice. Likewise, increases in potassium (5 to 13 molc m-3 NS) in the reproductive stage of the crop raised the concentrations of sugars, vitamin C, protein, lycopene, β-carotene and fruit juice. The concentration of carotenoids and the nutritional value of the tomato fruit were influenced by N and K nutrition by phenological stages, and these effects change slightly depending on the cluster harvested and the temperature during the growing cycle.


Afzal I, Hussain B, Ahmed-Basra SM, Ullah SH, Shakeel Q, Kamran M (2015). Foliar application of potassium improves fruit quality and yield of tomato plants. Acta Scientiarum Polonorum Hortorum Cultus 14(1):3-13.

Ahmad N, Sarfraz M, Farooq U, Arfan-ul-Haq M, Zaighum-Mushtaq M, Azhar-Ali M (2015). Effect of potassium and its time of application on yield and quality of tomato. International Journal of Scientific and Research Publications 5(9):1-4.

Almeselmani M, Pant RC, Singh B (2009). Potassium level and physiological response and fruit quality in hydroponically grown tomato. International Journal of Vegetable Science 16(1):85-99.

AOAC (2002). Official Methods of Analysis of AOAC International. Association of Official Analytical Chemists (17th ed), Washington DC.

Arah IK, Amaglo H, Kumah EK, Ofori H (2015). Preharvest and postharvest factors affecting the quality and shelf life of harvested tomatoes: a mini review. International Journal of Agronomy 2015:478041.

Balibrea ME, Martínez-Andújar C, Cuartero J, Bolarín MC, Pérez-Alfocea F (2006). The high fruit soluble sugar content in wild Lycopersicon species and their hybrids with cultivars depends on sucrose import during ripening rather than on sucrose metabolism. Functional Plant Biology 33(3):279-288.

Beckles DM (2012). Factors affecting the postharvest soluble solids and sugar content of tomato (Solanum lycopersicum L.) fruit. Postharvest Biology and Technology 63(1):129-140.

Bernardi ACC, Verruma-Bernardi MR (2013). Increases in yield and vitamin C levels of tomato grown on K2HPO4-enriched zeolite in an inert-sand substrate. e-ifc 33:10-13.

Beyers T, Vos C, Aerts R, Heyens K, Vogels L, Seels B, Höfte M, Cammue BPA, De Coninck B (2014). Resistance against Botrytis cinerea in smooth leaf pruning wounds of tomato does not depend on major disease signalling pathways. Plant Pathology 63(1):165-173.

Bhowmik D, Kumar KPS, Paswan S, Srivastava S (2012). Tomato-a natural medicine and its health benefits. Journal of Pharmacognosy and Phytochemistry 1(1):33-43.

Brandt S, Pek Z, Barna E, Lugasi A, Helyes L (2006). Lycopene content and color ripening tomatoes as affected by environmental conditions. Journal of the Science of Food and Agriculture 86(4):568-572.

Burton-Freeman B, Reimers K (2011). Tomato consumption and health: emerging benefits. American Journal of Lifestyle Medicine 5(2):182-191.

Caretto S, Parente A, Serio F, Santamaria P (2008). Influence of potassium and genotype on vitamin E content and reducing sugar of tomato fruits. HortScience 43(7):2048-2051.

Cebolla-Cornejo J, Roselló S, Valcárcel M, Serrano E, Beltrán J, Nuez F (2011). Evaluation of genotype and environment effects on taste and aroma flavor components of Spanish fresh tomato varieties. Journal of Agricultural and Food Chemistry 59(6):2440-2450.

Coskun D, Britto DT, Kronzucker HJ (2017). The nitrogen-potassium intersection: membranes, metabolism, and mechanism. Plant Cell and Environment 40(10):2029-2041.

Coyago-Cruz E, Corell M, Moriana A, Hernanz D, Benítez-González AM, Stinco CM, Meléndez-Martínez AJ (2018). Antioxidants (carotenoids and phenolics) profile of cherry tomatoes as influenced by deficit irrigation, ripening and cluster. Food Chemistry 240(1):870-884.

Dehnavard S, Souri MK, Mardanlu S (2017). Tomato growth responses to foliar application of ammonium sulfate in hydroponic culture. Journal of Plant Nutrition 40(3):315-323.

Dorais M, Ehret DL, Papadopoulos AP (2008). Tomato (Solanum lycopersicum) health components: from the seed to the consumer. Phytochemistry Reviews 7:231-250.

Dumas Y, Dadomo M, Lucca GD, Grolier P (2003). Effects of environmental factors and agricultural techniques on antioxidant content of tomatoes. Journal of the Science of Food and Agriculture 83(5):369-382.

Ehsan-Akhtar M, Zammer-Khan M, Tahir-Rashid M, Ahsan Z, Ahmad S (2010). Effect of potash application on yield and quality of tomato (Lycopersicon esculentum Mill.). Pakistan Journal of Botany 42(3):1695-1702.

Engels C, Kirkby E, White P (2012). Mineral nutrition, yield and source-sink relationships. In: Marschner P (Ed). Marschner’s Mineral Nutrition of Higher Plants. Academic Press, Elsevier Inc. San Diego, CA, USA pp 85-133.

Falqueto AR, Cassol D, Magalhaes-Júnior AM, Oliveira AC, Bacarin MA (2009). Physiological analysis of leaf senescence of two rice cultivars with different yield potential. Pesquisa Agropecuária Brasileira 44(7):695-700.

Fanasca S, Colla G, Maiani G, Venneria E, Rouphael Y, Azzini E, Saccardo F (2006). Changes in antioxidant content of tomato fruits in response to cultivar and nutrient solution composition. Journal of Agricultural and Food Chemistry 54(12):4319-4325.

FAO (2018). Food supply quantity (kg/capita/yr), tomatoes and products. Retrieved 2018 January 10 from

Faust F, Schubert S (2016). Protein synthesis is the most sensitive process when potassium is substituted by sodium nutrition of sugar beet (Beta vulgaris). Plant Physiology and Biochemistry 107:237-247.

Fujihara S, Kasuga A, Aoyagi Y (2001). Nitrogen-to-protein conversion factors for common vegetables in Japan. Journal of Food Science 66(3):412-415.

Ganeshamurthy AN, Satisha GC, Patil P (2011). Potassium nutrition on yield and quality of crops with special emphasis on banana and grapes. Karnataka Journal of Agricultural Science 24(1):29-38.

Gawel RR, Ewart AJW, Cirami R (2000). Effect of rootstock on must and wine composition and the sensory properties of Cabernet Sauvignon grown at Langhorne Creek, South Australia. Australian and New Zealand Wine Industry Journal 15:67-73.

Hawkesford M, Horst W, Kichey T, Lambers H, Schjoerring J, Moller IS, White P (2012). Functions of macronutrients. In: Marschner P (Ed). Marschner’s Mineral Nutrition of Higher Plants. Academic Press, Elsevier Inc. San Diego, CA, USA pp 135-189.

Hui Y, Hongxia C, Xinmei H, Lijie G, Hongzheng L, Xuanyi W (2017). Evaluation of tomato fruit quality response to water and nitrogen management under alternate partial root-zone irrigation. International Journal of Agricultural and Biological Engineering 10(5):85-94.

Jensen KH, Savage JA, Holbrook NM (2013). Optimal concentration for sugar transport in plants. Journal of the Royal Society Interface 20130055.

Jones B, Nachtsheim CJ (2009). Split-plot designs: what, why, and how. Journal of Quality Technology 41(4):340-361.

Jones JBJ (2008). Tomato plant culture in the field, greenhouse and home garden. CRC Press, Taylor and Francis Group (2nd ed), Boca Raton, FL.

Kanai S, Okhura K, Adu-Gyamfi JJ, Mohapatra PK, Nguyen NT, Saneoka H, Fujita K (2007). Depression of sink activity precedes the inhibition of biomass production in tomato plants subjected to potassium deficiency stress. Journal of Experimental Botany 58(11):2917-2928.

Kuehl OR (2000). Design of experiments: statistical principles of research design and analysis. Duxbury Press (2nd ed), Pacific Grove, CA.

Kuscu H, Turhan A, Ozmen N, Aydinol P, Demir AO (2014). Optimizing levels of water and nitrogen applied through drip irrigation for yield, and water productivity of processing tomato (Lycopersicon esculentum Mill.). Horticulture, Environment and Biotechnology 55:103-114.

Leghary SJ, Wahocho AN, Laghari GM, HafeezLaghari A, MustafaBhabhan G, HussainTalpur K, Bhutto TA, Wahocho SA, Lashari AA (2016). Role of nitrogen for plant growth and development: A review. Advances in Environmental Biology 10(9):209-218.

Lin CH, Chen BH (2003). Determination of carotenoids in tomato juice by liquid chromatography. Journal of Chromatography A 1012(1):103-109.

Lisko KA, Aboobucker SI, Torres R, Lorence A (2014). Engineering elevated vitamin C in plants to improve their nutritional content, growth, and tolerance to abiotic stress. In: Jetter R (Ed). Phytochemicals-Biosynthesis, Function and Application. Springer, International Publishing Switzerland, Switzerland, pp 109-128.

Liu J, Wu N, Wang H, Sun J, Peng B, Jiang P, Bai E (2016). Nitrogen addition affects chemical compositions of plant tissues, litter and soil organic matter. Ecology 97(7):1796-1806.

Mardanluo S, Souri MK, Ahmadi M (2018). Plant growth and fruit quality of two pepper cultivars under different potassium levels of nutrient solutions. Journal of Plant Nutrition 41(12):1604-1614.

Mengel K, Kirkby EA (2001). Principles of plant nutrition. Kluwer Academic Publishers (5th ed), Dordrecht, The Netherlands.

NNDSR (2018). Tomatoes, red, ripe, raw, year-round average. Basic Report 11529. USDA. Retrieved 2018 September 18 from

Oosterhuis DM, Loka DA, Kawakami EM, Pettigrew WT (2014). The physiology of potassium in crop production. Advances in Agronomy 126:203-233.

Parisi M, Giordano L, Pantangelo A, D’Onofrio B, Villari G (2006). Effects of different levels of nitrogen fertilization on yield and fruit quality in processing tomato. Acta Horticulturae 700:129-132.

Pourranjbari Saghaiesh S, Souri MK (2018). Root growth characteristics of Khatouni melon seedlings as affected by potassium nutrition. Acta Scientiarum Polonorum Hortorum Cultus 17(5):191-198.

Prudent M, Causse M, Génard M, Tripodi P, Granadillo S, Bertin N (2009). Genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on QTL detection. Journal of Experimental Botany 60(3):923-937.

Rajasree G, Pillai GR (2012). Effect of nitrogen nutrition on fruit quality and shelf life of cucurbitaceous vegetable bitter gourd. Journal of Plant Nutrition 35(8):1139-1153.

Ramírez SLF, Díaz SFR, Muro EJ (2012). Relation between soilless tomato quality and potassium concentration in nutritive solution. Acta Horticulturae 947:215-221.

San Martín-Hernández C, Ordaz-Chaparro VM, Sánchez-García P, Colinas-León MTB, Borges-Gómez L (2012). Tomato (Solanum lycopersicum L.) quality produced in hydroponics with different particle sizes of tezontle. Agrociencia 46(3):243-254.

SAS Institute Inc. (2011). SAS/STAT Users Guide. Version 9.3. SAS Institute Inc., Cary, N. C., USA.

Souri MK, Dehnavard S (2017). Characterization of tomato growth and fruit quality under foliar ammonium sprays. Open Agriculture 2(1):531-536.

Souri MK, Dehnavard S (2018) Tomato plant growth, leaf nutrient concentrations and fruit quality under nitrogen foliar applications. Advances in Horticultural Science 32(1):41-47.

Souri MK, Hatamian M (2019). Aminochelates in plant nutrition; a review. Journal of Plant Nutrition 42(1):67-78.

Souri MK, Rashidi M, Kianmehr MH (2018). Effects of manure-based urea pellets on growth, yield, and nitrate content in coriander, garden cress, and parsley plants. Journal of Plant Nutrition 41(11):1405-1413.

Souri MK, Sooraki FY, Moghadamyar M (2017). Growth and quality of cucumber, tomato, and green bean under foliar and soil applications of an aminochelate fertilizer. Horticulture, Environment, and Biotechnology 58(6):530-536.

Steiner AA (1961). A universal method for preparing nutrient solutions of a certain desired composition. Plant and Soil 15:134-154.

Taber H, Perkins-Veazie P, Li S, White W, Rodermel S, Xu Y (2008). Enhancement of tomato fruit lycopene by potassium is cultivar dependent. HortScience 43(1):159-165.

Tang G (2010). Bioconversion of dietary provitamin A carotenoids to vitamin A in humans. The American Journal of Clinical Nutrition 91(5):1468S-1473S.

Tohidloo G, Souri MK, Eskandarpour S (2018). Growth and fruit biochemical characteristics of three strawberry genotypes under different potassium concentrations of nutrient solution. Open Agriculture 3:356-362.

Vasák M, Schnabl J (2016). Sodium and potassium ions in proteins and enzyme catalysis. In: Sigel A, Sigel H, Sigel RKO (Eds). The Alkali Metal Ions: Their Role for Life. Metal Ions in Life Sciences. Springer, Cham, Switzerland, pp 259-290.

Vicente AR, Manganaris GA, Ortiz CM, Sozzi GO, Crisosto CH (2014). Nutritional quality of fruits and vegetables. In: Florkowski WJ, Shewfelt RL, Brueckner B, Prussia SE (Eds). Postharvest Handling: A System Approach. Academic Press (3rd ed), San Diego, CA pp 69-122.

Wang C, Gu F, Chen J, Yang H, Jiang J, Du T, Zhang J (2015). Assessing the response of yield and comprehensive fruit quality of tomato grown in greenhouse to deficit irrigation and nitrogen application strategies. Agricultural Water Management 161:9-19.

Wang M, Shen Q, Xu G, Guo S (2014). New insight into the strategy for nitrogen metabolism in plant cells. International Review of Cell and Molecular Biology 310:1-37.

Wang M, Zheng Q, Shen Q, Guo S (2013). The critical role of potassium in plant stress response. International Journal of Molecular Sciences 14(4):7370-7390.

Witham FH, Blaydes DF, Devlin RM (1971). Experiments in plant physiology. Van Nostrand Reinhold Co. New York, USA.

Yilmaz E (2001). The chemistry of fresh tomato flavor. Turkish Journal of Agriculture and Forestry 25:149-155.




How to Cite

SAN MARTÍN-HERNÁNDEZ, C., GÓMEZ-MERINO, F. C., SAUCEDO-VELOZ, C., QUINTANA-OBREGÓN, E. A., MUY-RANGEL, M. D., & TREJO-TÉLLEZ, L. I. (2021). Nitrogen and potassium supplied by phenological stages affect the carotenoid and nutritive content of the tomato fruit. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12320.



Research Articles
DOI: 10.15835/nbha49212320

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