Agronomical, physiological and molecular evaluation reveals superior salt-tolerance in bread wheat through salt-induced priming approach

Authors

  • Othman ALZAHRANI University of Tabuk, Faculty of Science, Biology Department, Tabuk;University of Tabuk, Faculty of Sciences, Genome and Biotechnology Unit, Tabuk (SA)
  • Heba ABOUSEADAA Ain Shams University, Faculty of Science, Botany Department, Giza (EG)
  • Taghreed K. ABDELMONEIM Molecular Genetics and Genome Mapping Laboratory, Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, 12619 (EG)
  • Mohammed A. ALSHEHRI University of Tabuk, Faculty of Science, Biology Department, Tabuk; University of Tabuk, Faculty of Sciences, Genome and Biotechnology Unit, Tabuk (SA)
  • Mohamed EL-MOGY Cairo University, Faculty of Agriculture, Vegetable Crops Department, 12613 Giza (EG)
  • Hossam S. EL-BELTAGI King Faisal University, Agricultural Biotechnology Department, College of Agriculture and Food Sciences, P.O. Box 420, Al-Ahsa 31982; Cairo University, Faculty of Agriculture, Biochemistry Department, Gamma St. Giza 12613 (SA)
  • Mohamed A. M. ATIA Molecular Genetics and Genome Mapping Laboratory, Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, 12619 (EG)

DOI:

https://doi.org/10.15835/nbha49212310

Keywords:

gene expression, priming, NaCl, salt, stress, Triticum aestivum, wheat

Abstract

Salt stress significantly limit wheat crop productivity worldwide. Exposure to non-lethal levels of salt stress, referred to as "salt-priming", allows plants to persist subsequent lethal conditions; the priming effect continues even after an extended salt stress-free period. This study attempted to evaluate the effectiveness of the salt-induced priming approach to cope with the toxic effects of long-term salinity stress in wheat. After 22 days of gradual salt acclamation to reach 250 mM NaCl, plants were recovered for eight days and finally shocked with 250 mM NaCl (priming+shock) for 7 days. After that, physiological parameters and gene expression of six salt-responsive genes were assessed. Additionally, 120 days after germination (at the end of the season), agronomic traits were recorded. Analysis of the agronomical traits revealed higher productivity in the salt-pretreated group (priming+shock) plants than the non-pretreated (shock only). Consistently, salt-pretreated plants maintained higher photosynthetic pigments level and decreased proline and MDA content than non-pretreated, suggesting enhanced salt tolerance. Moreover, salt-pretreated plants sustained high expressional levels of salt-responsive genes (TaNHX1, TaSOS1, TaSOS4, TaHKT1, TaHKT2, and TaAKT1) comparing with non-pretreated, indicating a vital role in ion homeostasis and conferring salt tolerance. Ultimately, this finding could facilitate novel smart approaches to improve wheat productivity under salt stress.

References

Abdelaziz ME, Abdelsattar M, Abdeldaym EA, Atia MA, Mahmoud AW, … Hirt H (2019). Piriformospora indica alters Na+/K+ homeostasis, antioxidant enzymes and LeNHX1 expression of greenhouse tomato grown under salt stress. Scientia Horticulturae 256:1-8. https://doi.org/10.1016/j.scienta.2019.05.059

Ahmadi J, Pour-Aboughadareh A, Ourang SF, Khalili P, Poczai P (2020). Unraveling salinity stress responses in ancestral and neglected wheat species at early growth stage: A baseline for utilization in future wheat improvement programs. Physiology and Molecular Biology of Plants 26:537-549. https://doi.org/10.1007/s12298-020-00768-4

Almansouri M, Kinet JM, Lutts S (1999). Compared effects of sudden and progressive impositions of salt stress in three durum wheat (Triticum durum Desf.) cultivars. Journal of Plant Physiology 154:743-752. https://doi.org/10.1016/S0176-1617(99)80253-3

Alshehri MA, Alzahrani O, Aziza AT, Alasmari A, Ibrahim S, … Alduaydi SA (2020). Correlation and genetic analyses of different characteristics in Saudi Arabian wheat reveal correlation networks and several trait-associated markers. Journal of Animal and Plant Science 30:1486-1497. https://doi.org/10.36899/JAPS.2020.6.0169

Assaha DV, Ueda A, Saneoka H, Al-Yahyai R, Yaish MW (2017). The role of Na+ and K+ transporters in salt stress adaptation in glycophytes. Frontiers in Physiology 8:509-527. https://doi.org/10.3389/fphys.2017.00509

Atia MA, Abdeldaym EA, Abdelsattar M, Ibrahim DS, Saleh I, … Abdelaziz ME (2020). Piriformospora indica promotes cucumber tolerance against Root-knot nematode by modulating photosynthesis and innate responsive genes. Saudi Journal of Biological Science 27:279-287. https://doi.org/10.1016/j.sjbs.2019.09.007

Barragán V, Leidi EO, Andrés Z, Rubio L, De Luca A, … Pardo JM (2012). Ion exchangers NHX1 and NHX2 mediate active potassium uptake into vacuoles to regulate cell turgor and stomatal function in Arabidopsis. The Plant Cell 24(3):1127-1142. https://doi.org/10.1105/tpc.111.095273

Benderradji L, Brini F, Amar SB, Kellou K, Azaza J, … Hanin M (2011). Sodium transport in the seedlings of two bread wheat (Triticum aestivum L.) genotypes showing contrasting salt stress tolerance. Australian Journal of Crop Science 5:233-241.

Bodirsky BL, Rolinski S, Biewald A, Weindl I, Popp A, Lotze-Campen H (2015). Global food demand scenarios for the 21 st century. PloS One 10(11):1-27. https://doi.org/10.1371/journal.pone.0139201

Brini F, Gaxiola RA, Berkowitz GA, Masmoudi K (2005). Cloning and characterization of a wheat vacuolar cation/proton antiporter and pyrophosphatase proton pump. Plant Physiology and Biochemistry 43:347-354. https://doi.org/10.1016/j.plaphy.2005.02.010

Brini F, Hanin M, Mezghani I, Berkowitz GA, Masmoudi K (2007). Overexpression of wheat Na+/H+ antiporter TNHX1 and H+-pyrophosphatase TVP1 improve salt-and drought-stress tolerance in Arabidopsis thaliana plants. Journal of Experimental Botany 58:301-308. https://doi.org/10.1093/jxb/erl251

Chaves MM, Oliveira MM (2004). Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. Journal of Experimental Botany 55:2365-2384. https://doi.org/10.1093/jxb/erh269

Chen Z, Pottosin II, Cuin TA, Fuglsang AT, Tester M, … Shabala S (2007). Root plasma membrane transporters controlling K+/Na+ homeostasis in salt-stressed barley. Plant Physiology 145:1714-1725. https://doi.org/10.1104/pp.107.110262

Cuin TA, Bose J, Stefano G, Jha D, Tester M, Mancuso S, Shabala S (2011). Assessing the role of root plasma membrane and tonoplast Na+/H+ exchangers in salinity tolerance in wheat: in planta quantification methods. Plant, Cell and Environment 34:947-961. https://doi.org/10.1111/j.1365-3040.2011.02296.x

Dawi F, El-Beltagi HS, Abdel-Mobdy YE, Salah SM, Ghaly IS, Abdel-Rahim EA, … Soliman AM (2021). Synergistic impact of the pomegranate peels and its nanoparticles against the infection of tobacco mosaic virus (TMV). Fresenius Environmental Bulletin 30(1):731-746.

Djanaguiraman M, Boyle DL, Welti R, Jagadish SV, Prasad PV (2018). Decreased photosynthetic rate under high temperature in wheat is due to lipid desaturation, oxidation, acylation, and damage of organelles. BMC Plant Biology 18:1-17. https://doi.org/10.1186/s12870-018-1263-z

Eissa HF, Hassanien SE, Ramadan AM, El-Shamy MM, Saleh OM, … Hassan SM (2017). Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance. Plant Methods 13(1):41-53. https://doi.org/10.1186/s13007-017-0191-5

El-Beltagi HS, Mohamed HI, Sofy MR (2020a). Role of ascorbic acid, glutathione and proline applied as singly or in sequence combination in improving chickpea plant through physiological change and antioxidant defense under different levels of irrigation intervals. Molecules 25:1702; https://doi.org/10.3390/molecules25071702

El-Beltagi HS, Sofy MR, Aldaej MI, Mohamed HI (2020b). Silicon alleviates copper toxicity in flax plants by up-regulating antioxidant defense and secondary metabolites and decreasing oxidative damage. Sustainability 12:4732. http://doi.org/10.3390/su12114732

El-Hendawy S, Al-Suhaibani N, Elsayed S, Alotaibi M, Hassan W, Schmidhalter U (2019). Performance of optimized hyperspectral reflectance indices and partial least squares regression for estimating the chlorophyll fluorescence and grain yield of wheat grown in simulated saline field conditions. Plant Physiology and Biochemistry 144:300-311. https://doi.org/10.1016/j.plaphy.2019.10.006

Elshafei AA, Afiah SA, Al-Doss AA, Ibrahim EI (2019). Morphological variability and genetic diversity of wheat genotypes grown on saline soil and identification of new promising molecular markers associated with salinity tolerance. Journal of Plant Interactions 14:564-571. https://doi.org/10.1080/17429145.2019.1672815

Farooq MA, Saqib ZA, Akhtar J (2015). Silicon-mediated oxidative stress tolerance and genetic variability in rice (Oryza sativa L.) grown under combined stress of salinity and boron toxicity. Turkish Journal of Agriculture and Forestry 39:718-729. http://journals.tubitak.gov.tr/agriculture/

Feki K, Quintero FJ, Pardo JM, Masmoudi K (2011). Regulation of durum wheat Na+/H+ exchanger TdSOS1 by phosphorylation. Plant Molecular Biology 76(6):545-556. https://doi.org/10.1007/s11103-011-9787-8

Filippou P, Tanou G, Molassiotis A, Fotopoulos V (2013). Plant acclimation to environmental stress using priming agents. In: Tuteja N, Singh Gill S (Eds). Plant Acclimation to Environmental Stress. Springer, New York pp 1-27.

Gálvez FJ, Baghour M, Hao G, Cagnac O, Rodríguez-Rosales MP, Venema K (2012). Expression of LeNHX isoforms in response to salt stress in salt sensitive and salt tolerant tomato species. Plant Physiology and Biochemistry 51:109-115. https://doi.org/10.1016/j.plaphy.2011.10.012

Gaxiola RA, Rao R, Sherman A, Grisafi P, Alper SL, Fink GR (1999). The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast. PNAS 96(4):1480-1485. https://doi.org/10.1073/pnas.96.4.1480

Godfray HC, Beddington JR, Crute IR, Haddad L, Lawrence D, … Toulmin C (2010). Food security: the challenge of feeding 9 billion people. Science 327:812-818. https://doi.org/10.1126/science.1185383

Gupta B, Huang B (2014). Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. International Journal of Genomics 2014:263-280. https://doi.org/10.1155/2014/701596.

Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000). Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51(1):463-499. https://doi.org/10.1146/annurev.arplant.51.1.463

Hasson E, Poljakoff-Mayber A, Gale J (1983). The effect of salt species and concentration on photosynthesis and growth of pea plants (Pisum sativum L. cv. Alaska). In: Marcelle R, Clijsters H, van Poucke M (Eds). Effects of Stress on Photosynthesis. Springer, Dordrecht pp 305-311.

Heath RL, Packer L (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125(1):189-98. https://doi.org/10.1016/0003-9861(68)90654-1

Ishitani M, Liu J, Halfter U, Kim CS, Shi W, Zhu JK (2000). SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding. The Plant Cell 12(9):1667-1677. https://doi.org/10.1105/tpc.12.9.1667

Jamil A, Riaz S, Ashraf M, Foolad MR (2011). Gene expression profiling of plants under salt stress.

Critical Reviews in Plant Sciences 30(5):435-458. https://doi.org/10.1080/07352689.2011.605739

Kanawapee N, Sanitchon J, Srihaban P, Theerakulpisut P (2013). Physiological changes during development of rice (Oryza sativa L.) varieties differing in salt tolerance under saline field condition. Plant and Soil 370(1-2):89-101. https://doi.org/10.1007/s11104-013-1620-5

Kosová K, Vítámvás P, Urban MO, Prášil IT (2013). Plant proteome responses to salinity stress–comparison of glycophytes and halophytes. Functional Plant Biology 40(9):775-786. https://doi.org/10.1071/FP12375

Kumar S, Beena AS, Awana M, Singh A (2017). Physiological, biochemical, epigenetic and molecular analyses of wheat (Triticum aestivum) genotypes with contrasting salt tolerance. Physiological, biochemical, epigenetic and molecular analyses of wheat (Triticum aestivum) genotypes with contrasting salt tolerance. Frontiers in Plant Science 8:1151-1570. https://doi.org/10.3389/fpls.2017.01151

Kumar S, Beena AS, Awana M, Singh A (2017). Salt-induced tissue-specific cytosine methylation downregulates expression of HKT genes in contrasting wheat (Triticum aestivum L.) genotypes. DNA Cell Biology 36(4):283-294. https://doi.org/10.1089/dna.2016.3505

Liu J, Ishitani M, Halfter U, Kim CS, Zhu JK (2000). The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance. Proceedings of the National Academy of Science 97:3730-3734. https://doi.org/10.1073/pnas.97.7.3730

Liu T, Zhuang L, Huang B (2019). Metabolic adjustment and gene expression for root sodium transport and calcium signaling contribute to salt tolerance in Agrostis grass species. Plant and Soil 443(1-2):219-232. https://doi.org/10.1007/s11104-019-04140-8

Majeed M, Khaneghah AM, Kadmi Y, Khan MU, Shariati MA (2018). Assessment of ochratoxin A in commercial corn and wheat products. Current Nutrition and Food Science 14(2):116-120. https://doi.org/10.2174/1573401313666170330155823

Mohammed AM, Diab MR, Abdelsattar M, Sayed MS (2017). Characterization and RNAi-mediated knockdown of Chitin Synthase A in the potato tuber moth, Phthorimaea operculella. Scientific Reports 7(1):1-12. https://doi.org/10.1038/s41598-017-09858-y

Mohamed HI, Akladious SA, El-Beltagi HS (2018). Mitigation the harmful effect of salt stress on physiological, biochemical and anatomical traits by foliar spray with trehalose on wheat cultivars. Fresenius Environmental Bulletin 27(10):7054-7065.

Mokhtar MM, Atia MA (2019). SSRome: an integrated database and pipelines for exploring microsatellites in all organisms. Nucleic Acids Research 47:244-252. https://doi.org/10.1093/nar/gky998

Oh DH, Lee SY, Bressan RA, Yun DJ, Bohnert HJ (2010). Intracellular consequences of SOS1 deficiency during salt stress. Journal of Experimental Botany 61(4):1205-1213. https://doi.org/10.1093/jxb/erp391

Paparella S, Araújo SS, Rossi G, Wijayasinghe M, Carbonera D, Balestrazzi A (2015). Seed priming: state of the art and new perspectives. Plant Cell Reports 34(8):1281-1293. https://doi.org/10.1007/s00299-015-1784-y

Parvaiz A, Satyawati S (2008). Salt stress and phyto-biochemical responses of plants-a review. Plant Soil and Environment 54:89-99. https://doi.org/10.17221/2774-PSE

Pervaiz Z, Afzal M, Xi S, Xiaoe Y, Ancheng L (2002). Physiological parameters of salt tolerance in wheat. Asian Journal of Plant Science 1:478-481. https://doi.org/10.3923/ajps.2002.478.481

Pitman MG, Läuchli A (2002). Global impact of salinity and agricultural ecosystems. In: Läuchli A, Lüttge U (Eds). Salinity: Environment-Plants-Molecules. Dordrecht, Netherlands pp 3-20.

Qados AM (2011). Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.). Journal of Saudi Society of Agricultural Science 10(1):7-15. https://doi.org/10.1016/j.jssas.2010.06.002

Ramezani A, Niazi A, Abolimoghadam AA, Babgohari MZ, Deihimi T, … Ebrahimie E (2013). Quantitative expression analysis of TaSOS1 and TaSOS4 genes in cultivated and wild wheat plants under salt stress. Molecular Biotechnology 53(2):189-197. https://doi.org/10.1007/s12033-012-9513-z

Rana V, Ram S, Nehra K, Sharma I (2016). Expression of genes related to Na+ exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress. Cereal Research Communications 44(3):404-413. https://doi.org/10.1556/0806.44.2016.009

Rao PS, Mishra B, Gupta SR (2013). Effects of soil salinity and alkalinity on grain quality of tolerant, semi-tolerant and sensitive rice genotypes. Rice Science 20(4):284-291. https://doi.org/10.1016/S1672-6308(13)60136-5

Saade S, Maurer A, Shahid M, Oakey H, Schmöckel SM, … Tester M (2016). Yield-related salinity tolerance traits identified in a nested association mapping (NAM) population of wild barley. Scientific Reports 6(1):1-9. https://doi.org/10.1038/srep32586

Sairam RK, Rao KV, Srivastava GC (2002). Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science 163(5):1037-1046. https://doi.org/10.1016/S0168-9452(02)00278-9

Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, … Jin H (2015). Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress. Scientific Reports 5:14278-14391. https://doi.org/10.1038/srep14278

Sani E, Herzyk P, Perrella G, Colot V, Amtmann A (2013). Hyperosmotic priming of Arabidopsis seedlings establishes a long-term somatic memory accompanied by specific changes of the epigenome. Genome Biology 14:59-81. https://doi.org/10.1186/gb-2013-14-6-r59

Sano N, Kim JS, Onda Y, Nomura T, Mochida K, … Seo M (2017). RNA-Seq using bulked recombinant inbred line populations uncovers the importance of brassinosteroid for seed longevity after priming treatments. Scientific Reports 7:1-4. https://doi.org/10.1038/s41598-017-08116-5

Saqib M, Zörb C, Rengel Z, Schubert S (2005). The expression of the endogenous vacuolar Na+/H+ antiporters in roots and shoots correlates positively with the salt resistance of wheat (Triticum aestivum L.). Plant Science 169(5):959-965. https://doi.org/10.1016/j.plantsci.2005.07.001

Schroeder JI, Delhaize E, Frommer WB, Guerinot ML, Harrison MJ, … Tsay YF (2013). Using membrane transporters to improve crops for sustainable food production. Nature 497(7447):60-66. https://doi.org/10.1038/nature11909

Sestak Z, Catsky J, Jarvis PG (1971). Plant photosynthetic production: A manual of methods. In: Sestak Z, Catsky J, Jarvis PG, Junk NV (Eds). Phytochemistry. The Hague, England pp 3-20.

Shabala S, Cuin TA (2008). Potassium transport and plant salt tolerance. Physiologia Plantarum 33(4):651-669. https://doi.org/10.1111/j.1399-3054.2007.01008.x

Shabnam N, Tripathi I, Sharmila P, Pardha-Saradhi P (2016). A rapid, ideal, and eco-friendlier protocol for quantifying proline. Protoplasma 253(6):1577-1582. https://doi.org/10.1007/s00709-015-0910-6

Shao T, Li L, Wu Y, Chen M, Long X, Shao H, Liu Z, Rengel Z (2016). Balance between salt stress and endogenous hormones influence dry matter accumulation in Jerusalem artichoke. Science of the Total Environment 568:891-898. https://doi.org/10.1016/j.scitotenv.2016.06.076

Shavrukov, Y (2013). Salt stress or salt shock: which genes are we studying? Journal of Experimental Botany 64:119-127. https://doi.org/10.1093/jxb/ers316

Shi H, Ishitani M, Kim C, Zhu JK (2000). The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter. PNAS 97(12):6896-6901.https://doi.org/10.1073/pnas.120170197

Shiferaw B, Prasanna BM, Hellin J, Bänziger M (2011). Crops that feed the world 6. Past successes and future challenges to the role played by maize in global food security. Food Security 3(3):307-327. https://doi.org/10.1007/s12571-011-0140-5

Shoresh M, Spivak M, Bernstein N (2011). Involvement of calcium-mediated effects on ROS metabolism in the regulation of growth improvement under salinity. Free Radical Biology and Medicine 51(6):1221-1234. https://doi.org/10.1016/j.freeradbiomed.2011.03.036

Soda N, Sharan A, Gupta BK, Singla-Pareek SL, Pareek A (2016). Evidence for nuclear interaction of a cytoskeleton protein (OsIFL) with metallothionein and its role in salinity stress tolerance. Scientific Reports 6:1-14. https://doi.org/10.1038/srep34762

Tari AF (2016). The effects of different deficit irrigation strategies on yield, quality, and water-use efficiencies of wheat under semi-arid conditions. Agricultural Water Management 167:1-10. https://doi.org/10.1016/j.agwat.2015.12.023

Varier A, Vari AK, Dadlani M (2010). The subcellular basis of seed priming. Current Science 25:450-456. https://www.jstor.org/stable/24109568

Wang Y, Wu WH (2013). Potassium transport and signaling in higher plants. Annual Review of Plant Biology 64:451-476. https://doi.org/10.1146/annurev-arplant-050312-120153

Xu Y, Zhou Y, Hong S, Xia Z, Cui D, … Jiang X (2013). Functional characterization of a wheat NHX antiporter gene TaNHX2 that encodes a K+/H+ exchanger. PLoS One 8:1-12. https://doi.org/10.1371/journal.pone.0078098

Yan M (2015). Seed priming stimulate germination and early seedling growth of Chinese cabbage under drought stress South African Journal of Botany 99:88-92. https://doi.org/10.1016/j.sajb.2015.03.195

Zeeshan M, Lu M, Naz S, Sehar S, Cao F, Wu F (2020). Resemblance and difference of seedling metabolic and transporter gene expression in high tolerance wheat and barley cultivars in response to salinity stress. Plants 9:519-535. https://doi.org/10.3390/plants9040519

Zhang T, Zhan X, Kang Y, Wan S, Feng H (2017). Improvements of soil salt characteristics and nutrient status in an impermeable saline–sodic soil reclaimed with an improved drip irrigation while ridge planting Lycium barbarum L. Journal of Soils and Sediments 17(4):1126-1139. https://doi.org/10.1007/s11368-016-1600-5

Zhao C, Zhang H, Song C, Zhu JK, Shabala S (2020). Mechanisms of plant responses and adaptation to soil salinity. The Innovation 21:1-41. https://doi.org/10.1016/j.xinn.2020.100017

Zhao Y, Li Y, Wang J, Pang H, Li Y (2016). Buried straw layer plus plastic mulching reduces soil salinity and increases sunflower yield in saline soils. Soil and Tillage Research 155:363-370. https://doi.org/10.1016/j.still.2015.08.019

Zhu JK (2000). Genetic analysis of plant salt tolerance using Arabidopsis. Plant Physiology 124(3):941-948. https://doi.org/10.1104/pp.124.3.941

Zhu JK (2003). Regulation of ion homeostasis under salt stress. Current Opinion in Plant Biology 6(5):441-445. https://doi.org/10.1016/s1369-5266(03)00085-2

Zou P, Li K, Liu S, He X, Zhang X, Xing R, Li P (2016). Effect of sulfated chitooligosaccharides on wheat seedlings (Triticum aestivum L.) under salt stress. Journal of Agricultural and Food Chemistry 64(14):2815-2821. https://doi.org/10.1021/acs.jafc.5b05624

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2021-05-10

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ALZAHRANI, O. ., ABOUSEADAA, H. ., ABDELMONEIM, T. K., ALSHEHRI, M. A., EL-MOGY, M. ., EL-BELTAGI, H. S., & ATIA, M. A. M. (2021). Agronomical, physiological and molecular evaluation reveals superior salt-tolerance in bread wheat through salt-induced priming approach. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12310. https://doi.org/10.15835/nbha49212310

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DOI: 10.15835/nbha49212310

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