Micropropagation, rooting, and acclimatization of two cultivars of goji (Lycium chinense)
In recent years, Lycium chinense (goji) has become increasing popular because of its public acceptance as a “superfood”. Hence, the present study aimed to develop a rapid production technology by using in vitro culture to produce plants with high health value, throughout year and in desired quantities. A micropropagation protocol for growing L. chinense ‘No 1’ and ‘New Big’ cultivars was developed. The explants were grown on MS medium supplemented with different concentrations of meta-Topolin (0.4-0.8 mg L-1), and WPM and RA without plant hormones. Among the tested combinations, the maximum regeneration rate (95-97%) with the mean shoot length of 3.53-4.12 cm and mean shoot number of 1.42–1.58 (‘No 1’ and ‘New Big’, respectively) was recorded for plants grown on MS with 0.6 mg L-1 mT and WPM. For in vitro rooting, healthy roots (4.71-4.91 cm) were obtained on MS with the addition of 20 ppm chitosan. A maximum of 70–80% plantlets (‘No 1’ and ‘New Big’, respectively) regenerated on the medium with chitosan were successfully acclimatized and established in the mixture of 90% peat and 10% perlite under field conditions.
Ait Barka E, Eullaffroy P, Clément C, Vernet G (2004). Chitosan improves development, and protects Vitis vinifera L. against Botrytis cinerea. Plant Cell Reports 22:608-614. https://doi.org/10.1007/s00299-003-0733-3
Anderson WC (1984). A revised tissue culture medium for shoot multiplication of rhododendron. Journal of the American Society for Horticultural Science 109(3):343-347.
Bairu MW, Stirk WA, Dolezal K, Van Staden J (2007). Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin?. Plant Cell, Tissue and Organ Culture 90(1):15-23. https://doi.org/10.1007/s11240-007-9233-4
Bartkowiak A (2001). Binary polyelectrolyte microcapsules based on natural polysaccharides. Edt. PS Szczecin.
Dănăilă-Guidea SM, Dobrinoiu RV, Vişan L, Toma RC (2015). Protocol for efficient in vitro multiplication of Lycium barbarum L.(goji) by direct organogenesis. Scientific Bulletin. Series F. Biotechnologies 19:34-38.
Fira A, Joshee N, Cristea V, Simu M, Hârța M, Pamfil D, Clapa D (2016). Optimization of micropropagation protocol for goji berry (Lycium barbarum L.). Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Horticulture 73(2). https://doi.org/10.15835/buasvmcn-hort:12177
Gentile A, Gutiérrez MJ, Martinez J, Frattarelli A, Nota P, Caboni E (2014). Effect of meta-Topolin on micropropagation and adventitious shoot regeneration in Prunus rootstocks. Plant Cell, Tissue and Organ Culture (PCTOC) 118(3):373-381. https://doi.org/10.1007/s11240-014-0489-1
Karakas FP (2020). Efficient plant regeneration and callus induction from nodal and hypocotyl explants of goji berry (Lycium barbarum L.) and comparison of phenolic profiles in calli formed under different combinations of plant growth regulators. Plant Physiology and Biochemistry 146:384-391. https://doi.org/10.1016/j.plaphy.2019.11.009
Kruczek A, Ochmian I, Krupa-Małkiewicz M, Lachowicz S (2020a). Comparison of morphological, antidiabetic and antioxidant properties of goji fruits. Acta Universitatis Cibiniensis. Series E: Food Technology 24(1):1-14. https://doi.org/10.2478/aucft-2020-0001
Kruczek A, Ochmian I (2016). The influence of shrubs cutting method on yielding and quality of the goji berries (Lycium barbarum L.). Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica 40(4 (330)):131-138. https://doi.org/10.2478/aucft-2020-0001
Kruczek A, Krupa-Małkiewicz M, Lachowicz S, Oszmiański J, Ochmian I (2020b). Health-promoting capacities of in vitro and cultivated goji (Lycium chinense Mill.) fruit and leaves; polyphenols, antimicrobial activity, macro-and microelements and heavy metals. Molecules 25(22):5314. https://doi.org/10.3390/molecules25225314
Krupa-Małkiewicz M, Fornal N (2018). Application of chitosan in vitro to minimize the adverse effects of salinity in Petunia × atkinsiana D. don. Journal of Ecological Engineering 19(1). https://doi.org/10.12911/22998993/79410
Kulczyński B, Gramza-Michałowska A (2016). Goji berry (Lycium barbarum): composition and health effects–a review. Polish Journal of Food and Nutrition Sciences 66(2):67-76. https://doi.org/10.1515/pjfns-2015-0040
Lloyd G, McCown B (1980). Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings of International Plant Propagators’ Society 30:421-427.
Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum 15:473-497.
Sá RR, da Cruz Caldas J, de Andrade Santana D, Lopes MV, Dos Santos WNL, Korn MGA, Júnior ADFS (2019). Multielementar/centesimal composition and determination of bioactive phenolics in dried fruits and capsules containing Goji berries (Lycium barbarum L.). Food Chemistry 273:15-23. https://doi.org/10.1016/j.foodchem.2018.05.124
Silvestri C, Sabbatini G, Marangelli F, Rugini E, Cristofori V (2018). Micropropagation and ex vitro rooting of Wolfberry. HortScience 53(10):1494-1499. https://doi.org/10.21273/HORTSCI13423-18
Sopalun K, Thammasiri K, Ishikawa K (2010). Effects of chitosan as the growth stimulator for Grammatophyllum speciosum in vitro culture. International Journal of Innovative Research in Science Engineering Technology 4(11):828-830.
Tabăra GM (2017). Aspects of the in vitro organogenesis of the species Lycium barbarum L. (Goji). Revista Botanică 14(1):29-34.
Naaz A, Hussain SA, Anis M, Alatar AA (2019). Meta-topolin improved micropropagation in Syzygium cumini and acclimatization to ex vitro conditions. Biologia Plantarum 63(1):174-182. https://doi.org/10.32615/bp.2019.020
Wojdyło A, Nowicka P, Bąbelewski P (2018). Phenolic and carotenoid profile of new goji cultivars and their anti-hyperglycemic, anti-aging and antioxidant properties. Journal of Functional Foods 48:632-642. https://doi.org/10.1016/j.jff.2018.07.061
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