Fertilization methods and substrate particle size differentially affect growth and macronutrient status of Laelia anceps subsp. anceps

  • Olga TEJEDA-SARTORIUS College of Postgraduates in Agricultural Sciences Campus Montecillo, Montecillo, State of Mexico (MX)
  • Yolanda L. FERNÁNDEZ-PAVÍA College of Postgraduates in Agricultural Sciences Campus Montecillo, Montecillo, State of Mexico (MX)
  • María G. PERALTA-SÁNCHEZ College of Postgraduates in Agricultural Sciences Campus Montecillo, Montecillo, State of Mexico (MX)
  • Libia I. TREJO-TÉLLEZ College of Postgraduates in Agricultural Sciences Campus Montecillo, Montecillo, State of Mexico (MX)
Keywords: foliar fertilization, growth parameters, nutrient uptake, orchid nutrition, orchid substrates


The effects of three fertilization methods (FM) using the Peters Professional® 30N-10P-10K water soluble fertilizer were analyzed: fertilization in irrigation water (FIW), foliar fertilization (FF), and their combination (FIW + FF), as well as two substrate particle sizes (SuPS): larger particle size (LPS) and smaller particle size (SPS), on the growth and macronutrient status of Laelia anceps subsp. anceps (Orchidaceae). A 3 × 2 factorial experiment was established. Aerial and root growth, dry weight and concentration of macronutrients N, P, K, Ca, and Mg in leaves, pseudobulbs, and roots were all evaluated. The interaction of the study factors resulted in a greater number of leaves, while by simple effects, greater leaf area, number of pseudobulbs, root volume, and dry matter of leaves and roots were obtained, when FIW and LPS were used. With FF, greater growth in root volume was observed. There were no differences in the concentration of N in the growth organs analyzed, but due to the interaction of factors, a higher concentration of P, K, Mg, and S was observed in roots, mainly with FIW + FF and SPS; although for P, there was a higher concentration in FIW and LPS. In the case of SPS, the concentrations of K and S were higher in leaves, and in pseudobulbs P, K, Ca, Mg, and S were higher. The results showed that there are positive effects on plant growth when FIW and LPS are used, while higher concentrations of nutrients in leaves, pseudobulbs and mainly in roots are observed when using SPS.


Metrics Loading ...


Alcántar GG, Sandoval MV (1999). Manual de análisis químico de tejido vegetal. Guía de muestreo, preparación, análisis e interpretación. Publicación Especial de la Sociedad Mexicana de la Ciencia del Suelo A. C. Chapingo, México.

Amberger-Ochsenbauer S (2010). Evaluation of growing media components for Phalaenopsis. Acta Horticulturae 878:355-360. https://doi.org/10.17660/ActaHortic.2010.878.44

Barman D, Naik SK (2017). Effect of substrate, nutrition and growth regulator on productivity and mineral composition of leaf and pseudobulb of Cymbidium hybrid “Baltic Glacier Mint Ice”. Journal of Plant Nutrition 40:784-794. https://doi.org/10.1080/01904167.2016.1201496

Berry ZC, Emery NC, Gotsh SG, Goldsmith GR (2019). Foliar water uptake: processes, pathways, and integration into plant water budgets. Plant, Cell & Environment 42:410-423. https://doi.org/10.1111/pce.13439

Bremner JM (1965). Total nitrogen. In Black CA (Ed). Methods of soil analysis. Part 2. Agronomy 9. American Society of Agronomy. Madison, WI, USA. pp 1149-1178.

Chase MW, Cameron KM, Freudenstein JV, Pridgeon AM, Salazar G, Van den Berg C, Schuiteman A (2015). An updated classification of Orchidaceae. Botanical Journal of the Linnean Society 177:151-174.

Díaz-Álvarez EA, Lindig-Cisneros R, de la Barrera E (2015). Responses to simulated nitrogen deposition by the neotropical epiphytic orchid Laelia speciosa. PeerJ 3:e1021. https://doi.org/10.7717/peerj.1021

Dressler RL (2005). How many orchid species? Selbyana 26:155-158.

Fageria NK, Barbosa MP, Moreira A, Guimarães (2009). Foliar fertilization of crop plants. Journal of Plant Nutrition 32:1044-1064. https://doi.org/10.1080/01904160902872826

García-Gaytán V, Valdovinos-Ponce G, Rodríguez-Mendoza MN, Pedraza-Santos ME, Trejo-Téllez LI, Soto-Hernández M (2013). Rutas de la penetración foliar en la fertilización de la orquídea Cymbidium sp. (Orchidaceae). Revista Mexicana de Ciencias Agrícolas Pub Esp:913-924.

Halbinger F, Soto M (1997). Laelias of Mexico. Orquídea (Mex). Herbario AMO, México.

Haytova D (2013). A review of foliar fertilization of some vegetables crops. Annual Review & Research in Biology 3(4):455-465.

Hew CS, Lim LY, Low CM (1993). Nitrogen uptake by tropical orchids. Environmental and Experimental Botany 33(2):273-281. https://doi.org/10.1016/0098-8472(93)90073-O

Hew CS, Yong WH (2004). The physiology of tropical orchids in relation to the industry. 2nd Ed. World Scientific, New Jersey.

Hwang SJ, Jeong BR (2007). Growth of Phalaenopsis plants in five different potting media. Journal of the Japanese Society of Horticultural Science 76(4):319-326.

Hwang SJ, Sivanesan I, Jeong BR (2009). Short-term ion uptake by Phalaenopsis as affected by concentration of the solution. Journal of Plant Nutrition 32(12):2044-2061. https://doi.org/10.1080/01904160903308143

Kannan S (2010). Foliar fertilization for sustainable crop production. In Lichtfouse E (Ed). Genetic engineering, biofertilisation, soil quality and organic farming. Sustainable Agriculture Reviews 4. Springer, Netherlands, pp 371-402. https://doi.org/10.1007/978-90-481-8741-6_13

Kim HJ, Kim J, Yun DL, Kim KS, Kim YJ (2016). Growth and flowering of Doritaenopsis Queen Beer “Mantefon” as affected by different potting substrates. The Horticulture Journal 85(4):360-365. https://doi.org/10.2503/hortj.MI-133

Lichty J, Singleton P, Kim HJ (2015). Substrates affect irrigation frequency and plant growth of potted orchids. Acta Horticulturae 1104:463-468. https://doi.org/10.17660/ActaHortic.2015.1104.66

Mantovani C, de Mello Prado R, Pivetta KFL (2015). Foliar diagnosis in Phalaenopsis orchid plants subjected to application of nitrogen. African Journal of Agricultural Research 10(53):4906-4912. https://doi.org/10.5897/AJAR2015.9921

Mengel K (2002). Alternative or complementary role of foliar supply in mineral nutrition. Acta Horticulturae 594:33-47. https://doi.org/10.17660/ActaHortic.2002.594.1

Naasz R, Caron J, Legault J, Pichette A (2009). Efficiency factors for bark substrates: Biostability, aeration, or phytotoxicity. Soil Science Society of America Journal 73(3):780-791. https://doi.org/10.2136/sssaj2008.0058

Nieves-Cordones M, García-Sánchez F, Pérez-Pérez JG, Colmenero-Flores JM, Rubio F, Rosales MA (2019). Coping with water shortage: An update on the role of K+, Cl-, and water membrane transport mechanisms on drought resistance. Frontiers in Plant Science 10:1619. https://doi.org/10.3389/fpls.2019.01619

Osorio AI, Osorio-Vega NW, Diez MC, Moreno FH (2014). Nutrient status and vegetative growth of Vanilla planifolia Jacks plants as affected by fertilization and organic substrate composition. Acta Agronómica 63(4):326-334. https://doi.org/10.15446/acag.v63n4.40754

Ruamrungsri S, Khuankaew T, Ohyama T, Sato T (2014). Nitrogen sources and its uptake in Dendrobium orchid by 15N tracer study. Acta Horticulturae 1025:207-211. https://doi.org/10.17660/ActaHortic.2014.1025.30

SAS (2011). SAS/STAT Users Guide. Version 9.3. SAS Institute Inc., Cary, N. C., USA.

Sedaghathoor S, Dehno GG, Naderi R, Kalatehjari S, Kaviani B (2017). Comparison of different media to produce Cymbidium orchids by pseudobulbs. Revista de Agricultura Neotropical 4:33-37.

Silva Júnior J, Rodrigues M, Castro EM, Bertolucci SK, Pasqual M (2013). Changes in anatomy and chlorophyll synthesis in orchids propagated in vitro in the presence of urea. Acta Scientiarum Agronomy 35(1):65-72. https://doi.org/10.4025/actasciagron.v35i1.15356

Slump K (2004). Predictions from the medium. Ingredients to use when making a potting mix for orchids. Orchids 73:416-418.

Susilo H, Peng Y-C, Lee S-C, Chen Y-C, Chang Y-CA (2013). The uptake and partitioning of nitrogen in Phalaenopsis Sogo Yukidian ‘V3’ as shown by 15N as a tracer. Journal of the American Society for Horticultural Science 138(3):229-237. https://doi.org/10.21273/JASHS.138.3.229

Tejeda-Sartorius O, Téllez-Velasco, MAA, Escobar-Aguayo JJ (2017). Estado de conservación de orquídeas silvestres (Orchidaceae). Agroproductividad 10(6):3-12.

Tejeda-Sartorius O, Trejo-Téllez LI, Téllez-Velasco MAA, Gómez-Merino FC (2018). Nutrient concentration in vegetative organs of the orchid Laelia anceps subsp. anceps based on mineral fertilization and biofertilization. The Horticultural Journal 87(4):541-548. https://doi.org/10.2503/hortj.OKD-148

Trejo-Téllez LI, Rodríguez-Mendoza MN, Gómez-Merino FC, Alcántar-González G (2016). Fertilización foliar. In Alcántar-González G, Trejo-Téllez LI, Gómez-Merino FC (Eds). Nutrición de cultivos. Segunda edición, Guadalajara, México pp 281-317.

Wang YT (2007). Potassium nutrition affects Phalaenopsis growth and flowering. HortScience 42(7):1563-1567. https://doi.org/10.21273/HORTSCI.42.7.1563

Wang YT (2008). High NO3-N to NH4-N ratios promote growth and flowering of a hybrid Phalaenopsis grown in two root substrates. HortScience 43(2):350-353. https://doi.org/10.21273/HORTSCI.43.2.350

Wang YT (2010). Phalaenopsis mineral nutrition. Acta Horticulturae 878:321-333. https://doi.org/10.17660/ActaHortic.2010.878.41

Wang YT, Blanchard M, Lopez R, Runkle E (2007). Growing the best Phalaenopsis. Part 2: Media, transplanting, water and nutrient requirements. Orchids 76:106-111.

Wang YT, Chang YCA (2017). Effects of nitrogen and the various forms of nitrogen on Phalaenopsis Orchid – A review. HortTechnology 27(2):144-149. https://doi.org/10.21273/horttech03204-16

Wang YT, Gregg LL (1994). Medium and fertilizer affect the performance of Phalaenopsis orchids during two flowering cycles. HortScience 29(4):269-271. https://doi.org/10.21273/HORTSCI.29.4.269

Wang YT, Konow EA (2002). Fertilizer source and medium composition affect vegetative growth and mineral nutrition of a hybrid moth orchid. Journal of the American Society for Horticultural Science 127(3):442-447. https://doi.org/10.21273/JASHS.127.3.442

Zhang S, Yang Y, Li J, Qin J, Zhang W, Huang W, Hu H (2018). Physiological diversity of orchids. Plant Diversity 40(4):196-208. https://doi.org/10.1016/j.pld.2018.06.003

Zong-min M, Ning Y, Shu-yun L, Hong H (2012). Nitrogen requirements for vegetative growth, flowering, seed production, and ramet growth of Paphiopedilum armeniacum (Orchid). HortScience 47(5):585-588. https://doi.org/10.21273/HORTSCI.47.5.585

Zotz G, Winkler U (2013). Aerial roots of epiphytic orchids: the velamen radicum and its role in water and nutrient uptake. Oecologia 171:733-741. https://doi.org/doi:10.1007/s00442-012-2575-6

How to Cite
TEJEDA-SARTORIUS, O., FERNÁNDEZ-PAVÍA, Y. L., PERALTA-SÁNCHEZ, M. G., & TREJO-TÉLLEZ, L. I. (2021). Fertilization methods and substrate particle size differentially affect growth and macronutrient status of Laelia anceps subsp. anceps. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12211. https://doi.org/10.15835/nbha49212211
Research Articles
DOI: 10.15835/nbha49212211

Most read articles by the same author(s)