Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities


  • Ivona VELIČKOVIĆ University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 3, 11000 Belgrade (RS)
  • Željko ŽIŽAK Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade (RS)
  • Nemanja RAJČEVIĆ University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 3, 11000 Belgrade (RS)
  • Marija IVANOV University of Belgrade, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, Bulevar Despota Stefana 142, 11000 Belgrade (RS)
  • Marina SOKOVIĆ University of Belgrade, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, Bulevar Despota Stefana 142, 11000 Belgrade (RS)
  • Petar D. MARIN University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 3, 11000 Belgrade (RS)
  • Slavica GRUJIĆ University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 3, 11000 Belgrade (RS)



anthocyanins; antioxidant activity; antitumor activity; enzyme-inhibitory activity; leaf extracts; Prunus spinosa


Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry.


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How to Cite

VELIČKOVIĆ, I., ŽIŽAK, Željko, RAJČEVIĆ, N., IVANOV, M., SOKOVIĆ, M., MARIN, P. D., & GRUJIĆ, S. (2021). Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12137.



Research Articles
DOI: 10.15835/nbha49112137