Chemical compositions and biological activities of the essential oils from gamma irradiated celery (Apium graveolens L.) seeds

  • Hossam S. EL-BELTAGI King Faisal University, College of Agriculture and Food Sciences, Agricultural Biotechnology Department, P.O. Box 420, Al-Ahsa 31982; Cairo University, Faculty of Agriculture, Biochemistry Department, Gamma st., Giza, 12613
  • Faten DHAWI King Faisal University, College of Agriculture and Food Sciences, Agricultural Biotechnology Department, P.O. Box 420, Al-Ahsa 31982
  • Amina A. ALY Egyptian Atomic Energy Authority, National Center for Radiation and Technology, Natural Products Department, Nasr City
  • Abeer E. EL-ANSARY Cairo University, Faculty of Agriculture, Biochemistry Department, Gamma st., Giza, 12613
Keywords: anticancer activity; antioxidant activity; Apium graveolens; gamma irradiation


Celery seed oils can help in detoxification processes of the body as it facilitates the elimination of toxins and other harmful substances out of the body. This study aimed to assess biological activity of essential oils extracted from un-irradiated and irradiated celery seeds by gamma rays. Celery seeds were irradiated with different dose levels of gamma radiation; control, 2.5, 5.0, and 10.0 kGy. The highest increase of essential oil extracted was registered with a dosage level of 10.0 kGy (2.42%). The GC-MS analysis showed that, there were new compounds detected in the irradiated samples and others compounds were increased or decreased. The FTIR spectra showed alterations in functional groups of essential oils extracted from celery seeds upon gamma-irradiation. Total phenols, total flavonoids and antioxidant activity increased proportional to the increase in the irradiation dose level and the maximum increase obtained in the irradiation dose level 10.0 kGy. This may be an evidence on the biological value, there were anticancer activity against 2 cell lines, breast cancer cell line (MCF-7) and lung cancer cell line (A549). The best IC50 in A549 was 145 µg/ml in sample with irradiation dose level 5.0 kGy while the best IC50 in MCF-7 was 124 µg/ml in irradiated sample with dose 5.0 kGy. Also, the highest inhibition zone of S. aureus, B. subtilis and K. pneumoniae were obtained with the oil extracted from seeds irradiated with 5 kGy dose level, where E. coli and C. albicans were more pronounced for the oil gotten from the seeds irradiated with 10.0 kGy. The irradiation process for celery seeds may be promising for biological value of celery oils.


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Abdeldaiem MH, Mohamed HG, Abdel-Khalek HH (2009). Antimicrobial and antioxidant activities of rosemary essential oil treated by gamma irradiation. Journal of Radiation Research and Applied Sciences 2(4):819-837.

Afify AEMM, El-Beltagi HS, Aly AA, El-Ansary AE (2012). Antioxidant enzyme activities and lipid peroxidation as biomarker compounds for potato tuber stored by gamma radiation. Asian Pacific Journal of Tropical Biomedicine 2(3):S1548-S1555.

Afify AMR, Rashed MM, Ebtesam AM, El-Beltagi HS (2013). Effect of gamma radiation on the lipid profiles of soybean, peanut and sesame seed oils. Grasas y Aceites 64(4):356-368.

Ahmedy O (2016). Study of the anticancer potential of celery seed oil against chemically induced hepatocellular carcinoma in rats: a mechanistic approach. Al-Azhar Journal of Pharmaceutical Sciences 53(1):14-28.

Ali SI, El-Emary GAE, Mohamed AA (2018). Effects of gamma irradiation on FT-IR fingerprint, phenolic contents and antioxidant activity of Foeniculum vulgare and Carum carvi seeds. Research Journal of Pharmacy and Technology 11(8):3323-3329.

Alloun K, Benchabane O, Hazzit M, Mouhouche F, Baaliouamer A, Chikhoune A, Benchabane A (2019). Effect of gamma ray irradiation on chemical composition, antioxidant, antimicrobial, and insecticidal activities of Thymus pallescens essential oil. Acta Chromatographica 31:57-62.

Aly AA, EI-Beltagi HE (2010). Influence of ionizing radiation on the antioxidant enzymes of Vicia faba L. Grasas y Aceites 61(3):288-294.

Aly AA, Eliwa NE, AbdEl-Megid MH (2019). Stimulating effect of gamma radiation on some active compounds in eggplant fruits. Egyptian Journal of Radiation Sciences and Applications 32:61-73.

Aly AA, Mansour TMM, Mohamed HI, Abd-Elsalam KA (2012). Examination of correlations between several biochemical components and powdery mildew resistance of flax cultivars. Plant Pathology Journal 28(2):149-155.

Aly AA, Maraei RW, Ali HGM (2016). Fatty acids profile and chemical composition of Egyptian Moringa oleifera seed oils. Journal of the American Oil Chemists Society 93:397-404.

Ameer K, Jo Y, Amir RM, Shahbaz HM, Kwon JH (2020). Screening and identification of electron-beam irradiated dried spice-mixture products by electronic sensing and standard analytical methods through dose estimation. LWT 125:108957.

Aprotosoaie AC, Costache II, Miron A (2016). Anethole and its role in chronic diseases. In: Drug Discovery from Mother Nature Springer, Cham. pp 247-267.

Araújo CRR, Corrêa GM, da Costa Abreu VG, de Melo Silva T, Osorio AMB, De Oliveira PM, de Carvalho Alcântara AF (2017). Effects of gamma radiation on essential oils: A review. New Insights on Gamma Rays 179-201.

Balakrishnan R, Elangovan N, Mohankumar T, Nataraj J, Manivasagam T, Thenmozhi AJ, … Khan MAS (2018). Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis. Frontiers in Bioscience 10:248-261.

Bauer AW (1966). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45(4):4963-496.

Bejeshk MA, Fekri MS, Najafipour H, Rostamzadeh F, Jafari E, Rajizadeh MA, Masoumi-Ardakani Y (2019). Anti-inflammatory and anti-remodeling effects of myrtenol in the lungs of asthmatic rats: Histopathological and biochemical findings. Allergologia et Immunopathologia 47(2):185-193.

Bobadilla D, Flores-Giubi ME, Alvarenga N (2019). Synergistic effect of (s)-cis-verbenol with the antibiotic’s amoxicillin and gentamicin against sensitive and resistant Staphylococcus aureus strains. International Journal of Pharmaceutical Sciences and Research 10(5):2182-2188.

Boughendjioua H, Djeddi S (2014). Biological activity of lemon essential oil used for skin care. European Journal of Scientific Research l:219-226.

British Pharmacopoeia Commission (1993). British Pharmacopoeia.

Chavan MJ, Wakte PS, Shinde DB (2010). Analgesic and anti-inflammatory activity of caryophyllene oxide from Annona squamosa L. bark. Phytomedicine 17(2):149-151.

Dąbrowska JA, Kunicka-Styczyńska A, Śmigielski KB (2020). Biological, chemical, and aroma profiles of essential oil from waste celery seeds (Apium graveolens L.). Journal of Essential Oil Research 32:308-315.

de Almeida Melo E, Mancini Filho J, Guerra NB (2005). Characterization of antioxidant compounds in aqueous coriander extract (Coriandrum sativum L.). LWT - Food Science and Technology 38(1):15-19.

do Nascimento KF, Moreira FMF, Santos JA, Kassuya CAL, Croda JHR, Cardoso CAL, … Formagio ASN (2018). Antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of Psidium guineense Sw. and spathulenol. Journal of Ethnopharmacology 210:351-358.

Dolati K, Rakhshandeh H, Golestani M, Forouzanfar F, Sadeghnia R, Sadeghnia HR (2018). Inhibitory effects of Apium graveolens on xanthine oxidase activity and serum uric acid levels in hyperuricemic mice. Preventive Nutrition and Food Science 23(2):127-133.

Douar-Latreche S, Benchabane O, Sahraoui N, Hazzit M, Mouhouche F, Baaliouamer A (2018). Effect of gamma irradiation on the chemical composition and antioxidant activity of Thymus algeriensis extracts. Journal of Essential Oil-Bearing Plants 21(2):449-461.

Ehiabhi OS, Edet UU, Walker TM, Schmidt JM, Setzer WN, Ogunwande IA, … Ekundayo O (2013). Constituents of essential oils of Apium graveolens L., Allium cepa L., and Voacanga africana Staph. from Nigeria. Journal of Essential Oil-Bearing Plants 9(2):126-132.

El-Beltagi HES (2011). Effect of roasting treatments on protein fraction profiles, some enzyme activities of Egyptian peanuts. International Journal of Food Science and Nutrition 62(5):453-456.

El-Beltagi HS, Ahmed OK, El-Desouky W (2011). Effect of low doses γ-irradiation on oxidative stress and secondary metabolites production of rosemary (Rosmarinus officinalis L.) callus culture. Radiation Physics and Chemistry 80(9):968-976.

El-Beltagi HS, Aly AA, El-Desouky W (2019b). Effect of gamma irradiation on some biochemical properties, antioxidant and antimicrobial activities of Sakouti and Bondoky dry dates fruits genotypes. Journal of Radiation Research and Applied Sciences 12(1):437-446.

El-Beltagi HS, Mohamed HI, Megahed BMH, Gamal M, Safwat G (2018). Evaluation of some chemical constituents, antioxidant, antibacterial and anticancer activities of Beta vulgaris L. root. Fresenius Environmental Bulletin 27(9):6369-6378.

El-Demerdash E (2011). Anti-inflammatory and antifibrotic effects of methyl palmitate. Toxicology and Applied Pharmacology 254(3):238-244.

Elzey B, Norman V, Stephenson J, Pollard D, Fakayode SO (2016). Purity analysis of adulterated essential oils by FT-IR spectroscopy and partial-least-squares regression. Spectroscopy 31:26-37.

Ene-Obong H, Onuoha N, Aburime L, Mbah O (2018). Chemical composition and antioxidant activities of some indigenous spices consumed in Nigeria. Food Chemistry 238:58-64.

Ezhilan BP, Neelamegam R (2012). GC-MS analysis of phytocomponents in the ethanol extract of Polygonum chinense L. Pharmacognosy Research 4(1):11.

Fatemi F, Dadkhah A, Rezaei MB, Dini S (2013). Effect of γ-irradiation on the chemical composition and antioxidant properties of cumin extracts. Journal of Food Biochemistry 37(4):432-439.

Fernandes ES, Passos GF, Medeiros R, da Cunha FM, Ferreira J, Campos MM, … Calixto JB (2007). Anti-inflammatory effects of compounds alpha-humulene and (−)-trans-caryophyllene isolated from the essential oil of Cordia verbenacea. European Journal of Pharmacology 569(3):228-236.

Fu J, Chen D, Zhao B, Zhao Z, Zhou J, Xu Y, Yin Z (2012). Luteolin induces carcinoma cell apoptosis through binding Hsp90 to suppress constitutive activation of STAT3. PloS One 7(11):e49194.

Gauri M, Ali SJ, Khan MS (2015). A review of Apium graveolens (Karafs) with special reference to Unani medicine. International Archives of Integrated Medicine 2:131-136.

Gutiérrez LF, Quiñones-Segura Y, Sanchez-Reinoso Z, Díaz DL, Abril JI (2017). Physicochemical properties of oils extracted from γ-irradiated sacha inchi (Plukenetia volubilis L.) seeds. Food Chemistry 237:581-587.

Hadjadj N, Hazzit M (2020). Analysis and antioxidant activity of essential oils and methanol extracts of Origanum floribundum Munby. Journal of Essential Oil-Bearing Plants 23(1):85-96.

Hassan AB, Ahmed SM, Elkhatim SKA, Abdelhalim TS, Fawale SO, Adiamo OQ, Ahmed MIA (2019). Effect of gamma irradiation and microwave heating treatments on microbial load and antioxidant potentials in cinnamon, fennel and hot pepper. Journal of Food Measurement and Characterization13(2):1130-1138. 45

Hassanen NH, Eissa AMF, Hafez SAM, Mosa EA (2015). Antioxidant and antimicrobial activity of celery (Apium graveolens) and coriander (Coriandrum sativum) herb and seed essential oils. International Journal of Current Microbiology and Applied Sciences 4(3):284-296.

Hikima Y, Morikawa J, Kazarian SG (2019). Analysis of molecular orientation in polymeric spherulite using polarized micro attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic imaging. Analytica Chimica Acta 1065:79-89.

Horváthov J, Suhaj M, Polovka M, Brezová V, Šimko P (2007). The influence of gamma-irradiation on the formation of free radicals and antioxidant status of oregano (Origanum vulgare L.). Czech Journal of Food Sciences 25(3):131-143.

Ibrahim HK (2016). Antibacterial and antioxidant activity of seed methanolic extract of Apium graveolens in vitro. World Journal of Pharmaceutical Research 5(6):1914-1923.

Ingallina C, Capitani D, Mannina L, Carradori S, Locatelli M, Di Sotto A, … Sobolev AP (2019). Phytochemical and biological characterization of Italian “sedano biancodi di Sperlonga” Protected Geographical Indication celery ecotype: a multimethodological approach. Food Chemistry 309:125649.

Javadi I, Rashidi Nooshabadi M, Goudarzi M, Roudbari R (2015). Protective effects of celery (Apium graveloens) seed extract on bleomycin-induced pulmonary fibrosis in rats. Journal of Babol University of Medical Sciences 17(1):70-76.

Kesba HH, El-Beltagi HS (2012). Biochemical changes in grape rootstocks resulted from humic acid treatments in relation to nematode infection. Asian Pacific Journal of Tropical Biomedicine 2(4):287-293.

Kirkin C, Mitrevski B, Gunes G, Marriot PJ (2014). Combined effects of gamma-irradiation and modifed atmosphere packaging on quality of some species. Food Chemistry 154:255-261.

Kizilay Z, Kahraman Cetin N (2018). Effect of methyl palmitate on the formation of epidural fibrosis in an experimental epidural fibrosis model. Journal of Investigative Surgery 31(6):469-474.

Köken T, Koca B, Özkurt M, Erkasap N, Kuş G, Karalar M (2016). Apium graveolens extract inhibits cell proliferation and expression of vascular endothelial growth factor and induces apoptosis in the human prostatic carcinoma cell line LNCaP. Journal of Medicinal Food 19(12):1166-1171.

Lima F, Vieira K, Santos M, de Souza PM (2018). Effects of radiation technologies on food nutritional quality. Descriptive Food Science 137-152.

Lin LY, Ker YB, Chang CH, Chen KC, Peng RY (2011). Arabinogalactan present in the mountain celery seed extract potentiated hypolipidemic bioactivity of coexisting polyphenols in hamsters. Pharmaceutical Biology 49(3):319-326.

Lone ZA, Lone Y, Khan SS, Wani AA, Reshi MI (2015). Hepatoprotective medicinal plants used by the Gond and Bhill tribals of District Raisen, Madhya Pradesh, India. Journal of Medicinal Plant Research 9:400-406.

Lu ZG, Li W, Wang PJ (2011). Chemical composition and ability of scavenging DPPH radical of essential oil and residue from the celery seed. In: Advanced Materials Research 183:18-21. Trans Tech Publications LTD.

Luckman GJ (2002). Food irradiation: regulatory aspects in the Asia and Pacific region. Radiation Physics and Chemistry 63(3-6):285-288.

Mahindru SN (2005). Food preservation and irradiation. 1st Ed., Saujanya Books, New Delhi, India.

Mansouri E, Kooti W, Bazvand M, Boroon MG, Amirzargar A, Afrisham R, … Jalali N (2015). The effect of hydro-alcoholic extract of Foeniculum vulgare Mill on leukocytes and hematological tests in male rats. Jundishapur Journal of Natural Pharmaceutical Products 10(1):e18396.

Marei GIK, Rasoul MAA, Abdelgaleil SA (2012). Comparative antifungal activities and biochemical effects of monoterpenes on plant pathogenic fungi. Pesticide Biochemistry and Physiology 103(1):56-61.

McCormick. The History of Spices (2017). Retrieved 2017 May 30 from:

Mulyaningsih S, Sporer F, Zimmermann S, Reichling J, Wink M (2010). Synergistic properties of the terpenoids aromadendrene and 1, 8-cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibiotic-resistant pathogens. Phytomedicine 17(13):1061-1066.

Nickavar B, Kamalinejad M, Izadpanah H (2007). In vitro free radical scavenging activity of five Salvia species. Pakistan Journal of Pharmaceutical Sciences 20(4):291-294.

Nijveldt RJ, van Nood ELS, van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA (2001). Flavonoids: a review of probable mechanisms of action and potential applications. American Journal of Clinical Nutrition 74:418-425.

Oyedemi SO, Okoh AI, Mabinya LV, Pirochenva G, Afolayan AJ (2009). The proposed mechanism of bactericidal action of eugenol, -terpineol and -terpinene against Listeria monocytogenes, Streptococcus pyogenes, Proteus vulgaris and Escherichia coli. African Journal of Biotechnology 8:1280-1286.

Park HR, Park E, Rim AR, Jeon KI, Hwang JH, Lee SC (2006). Antioxidant activity of extracts from Acanthopanax senticosus. African Journal of Biotechnology 5(23):2388-2396.

Park SH, Ham S, Kwon TH, Kim MS, Lee DH, Kang JW, Yoon DY (2014). Luteolin induces cell cycle arrest and apoptosis through extrinsic and intrinsic signaling pathways in MCF-7 breast cancer cells. Journal of Environmental Pathology, Toxicology, and Oncology 33(3):219-231.

Paula-Freire LIGD, Andersen ML, Gama VS, Molska GR Carlini ELA (2014). The oral administration of trans-caryophyllene attenuates acute and chronic pain in mice. Phytomedicine 21(3):356-362.

Pereira E, Pimenta AI, Barros L, Calhelha RC, Antonio AL, Verde SC, Ferreira IC (2018). Effect of gamma radiation on the bioactivity of medicinal and aromatic plants: Mentha xpiperita L., Thymus vulgaris L. and Aloysia citrodora palau as case studies. Food and Function 10:5156-5161.

Powanda MC, Whitehouse MW, Rainsford KD (2015). Celery seed and related extracts with antiarthritic, antiulcer, and antimicrobial activities. In: Novel Natural Products: Therapeutic Effects in Pain, Arthritis and Gastro-intestinal Diseases. Springer, Basel pp 133-153.

Pozzatti P, Rigatti F, Hörner R, Alves SH, Mallmann CA, Heinzmann BM (2015). Antimicrobial evaluation of sesquiterpene alpha-curcumene and its synergism with imipenem. Journal of Microbiology, Biotechnology and Food Sciences 4(5):434.

Quassinti L, Maggi F, Barboni L, Ricciutelli M, Cortese M, Papa F, Bramucci M (2014). Wild celery (Smyrnium olusatrum L.) oil and isofuranodiene induce apoptosis in human colon carcinoma cells. Fitoterapia 97:133-141.

Quintans-Júnior L, Moreira JC, Pasquali MA, Rabie S, Pires AS, Schröder R, … Gelain DP (2013). Antinociceptive activity and redox profile of the monoterpenes (+)-camphene, p-cymene, and geranyl acetate in experimental models. International Scholarly Research Notices Toxicology 2013:459530.

Rajizadeh MA, Najafpour H, Samareh Fekri M, Rostamzadeh F, Jafari E, Bejeshk MA (2019). Anti-inflammatory and anti-oxidative effects of myrtenol in the rats with allergic asthma. Iranian Journal of Pharmaceutical Research 18(3):1488-1498. Https://

Repetto G, del Peso A, Zurita JL (2008). Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature Protocols 3:1125-1131.

Rezanejad R, Ojagh SM, Heidarieh M, Raeisi M, Rafiee G, Alishahi A (2019). Characterization of gamma-irradiated Rosmarinus officinalis L. (Rosemary). Turkish Journal of Pharmaceutical Sciences 16(1):43-47.

Rezk AA, Al-Khayri JM, Al-Bahrany AM, El-Beltagi HS, Mohamed HI (2019). X-ray irradiation changes germination and biochemical analysis of two genotypes of okra (Hibiscus esculentus L). Journal of Radiation Research and Applied Sciences 12(1):393-402.

Romanova D, Vachalkova A, Cipak L, Ovesna Z, Rauko P (2001). Study of antioxidant effect of apigenin, luteolin and quercetin by DNA protective method. Neoplasma 48:104-107.

Rossi PG, Bao L, Luciani A, Panighi J, Desjobert JM, Costa J, Berti L (2007). (E)-Methylisoeugenol and elemicin: antibacterial components of Daucus carota L. essential oil against Campylobacter jejuni. Journal of Agricultural and Food Chemistry 55(18):7332-7336.

Saki K, Bahmani M, Rafieian-Kopaei M (2014). The effect of most important medicinal plants on two importnt psychiatric disorders (anxiety and depression)-a review. Asian Pacific Journal of Tropical Biomedicine 7:S34-S42.

Salehi B, Venditti A, Frezza C, Yücetepe A, Altunta Ü, Uluata S, Matthews RK (2019). Apium plants: Beyond simple food and phytopharmacological applications. Applied Science 9(17):3547.

Seo HY, Kim JH, Song HP, Kim DH, Byun MW, Kwon JH, Kim KS (2007). Effects of gamma irradiation on the yields of volatile extracts of Angelica gigas Nakai. Radiation Physics and Chemistry 76:1869-1874.

Shakir MA (2013). Extraction and purification flavonoid from celery plant and application on hepatic and breast. International Journal of Current Research 5:2462-2465.

Sharawy MH, El-Agamy DS, Shalaby AA, Ammar ESM (2013). Protective effects of methyl palmitate against silica-induced pulmonary fibrosis in rats. International Immunopharmacology 16(2):191-198.

Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3):144-158.

Subhadradevi V, Khairunissa K, Asokkumar K, Sivashanmugam MUA, Jagannath P (2011). Induction of apoptosis and cytotoxic activities of Apium graveolens Linn. using in vitro models. Middle East Journal of Scientific Research 9(1):90-94.

Tashakori-Sabzevar F, Razavi BM, Imenshahidi M, Daneshmandi M, Fatehi H, Sarkarizi YE, Mohajeri SA (2016). Evaluation of mechanism for antihypertensive and vasorelaxant effects of hexanic and hydroalcoholic extracts of celery seed in normotensive and hypertensive rats. Revista Brasileira de Farmacognosia 26(5):619-626.

Teixeira B, Marques A, Ramos C, Neng NR, Nogueira JM, Saraiva JA, Nunes ML (2013). Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Industrial Crops and Products 43:587-595.

Topal F (2019). Anticholinergic and antidiabetic effects of isoeugenol from clove (Eugenia caryophylata) oil. International Journal of Food Properties 22(1):583-592.

Vallianou I, Peroulis N, Pantazis P, Hadzopoulou-Cladaras M (2011). Camphene, a plant-derived monoterpene, reduces plasma cholesterol and triglycerides in hyperlipidemic rats independently of HMG-CoA reductase activity. PloS One 6(11):e20516.

Wistriech GA (1997). Microbiology laboratory. pp 319-325.

Wu SY, Shen JL, Man KM, Lee YJ, Chen HY, Chen YH, … Chen WC (2014). An emerging translational model to screen potential medicinal plants for nephrolithiasis, an independent risk factor for chronic kidney disease. Evidence-based Complementary and Alternative Medicine 2014:972958.

Yalcin H, Ozturk I, Tulukcu E, Sagdic O (2011). Effect of γ-irradiation on bioactivity, fatty acid compositions and volatile compounds of Clary sage seed (Salvia sclarea L.). Journal of Food Science 76(7):C1056-C1061.

Zhishen J, Mengcheng T, Jianming W (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4):555-559.

How to Cite
EL-BELTAGI, H. S., DHAWI, F., ALY, A. A., & EL-ANSARY, A. E. (2020). Chemical compositions and biological activities of the essential oils from gamma irradiated celery (Apium graveolens L.) seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(4), 2114-2133.
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