Genetic relationship of mungbean and blackgram genotypes based on agronomic and photosynthetic performance and SRAP markers
Genetic identification is at the base of parental selection in varietal development programs. Agronomic and photosynthetic characters and sequence-related amplified polymorphism (SRAP) markers were analyzed for two legume species which included 23 mungbean (Vigna radiata (L.) Wilczek) and four blackgram (Vigna mungo (L.) Hepper) genotypes. The results revealed that the seeds/pod, plant height, pods/plant, pod length, days to flowering, and all photosynthetic characters studied had a significant correlation with the yield/plant. Using UPGMA analysis with phenotypic data, five clusters and two individuals were identified. Twenty-five SRAP primer combinations generated 562 amplified bands, of which 507 were polymorphic (90.2%). The average numbers of scorable and polymorphic bands/primer pair were 22 and 20, respectively. Two major clusters coincided with two species having a 100% bootstrap value. Within the mungbean cluster, there were two subclusters containing 12 and 11 mungbean genotypes. Mantel’s test demonstrated that the polymorphisms given by SRAPs were associated with agronomic and photosynthetic variability (r = 0.734, p < 0.01). These results allow promising mungbean genotypes to be identified through genetic diversity and field performance which can be utilized as potential parents towards future breeding programs. Moreover, the factors which contribute most to yield/plant can be simultaneously used as selection criteria for yield improvement.
Alghamdi SS, Al-Faifi SA, Migdadi HM, Al-Rowaily SL, El-Harty EH, Farooq M (2017). Genetic diversity and field performance of mung bean, faba bean and lentil genotypes in the kingdom of Saudi Arabia. International Journal of Agriculture and Biology 19:689-696. https://doi.org/10.17957/IJAB/15.0336
Aneja B, Yadav NR, Yadav RC, Kumar R (2013). Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]. Physiology and Molecular Biology of Plants 19:399-407. https://doi.org/10.1007/s12298-013-0177-3
Ballon FB, Legaspi BM, Catipon EM (1978). Mungbean varietal improvement program of the bureau of plant industry. In: Cowell R (Ed). The First International Mungbean Symposium. AVRDC, Shanhua, Taiwan pp 217-219.
Bassam BJ, Gresshoff PM (2007). Silver staining DNA in polyacrylamide gels. Nature Protocols 2:2649-2654. https://doi.org/10.1038/nprot.2007.330
Catipon EM, Legaspi BM, Jarilla FA (1988). Development of mungbean varieties from AVRDC lines for the Philippines. In: Shanmugasundaram S, Mclean BT (Eds). Mungbean: Proceedings of the Second International Symposium. AVRDC, Shanhua, Taiwan pp 88-97.
Chai Nat Field Crops Research Center (2018). Guidelines for data record in mungbean research. Chai Nat Field Crops Research Center, Field and Renewable Energy Crops Research Institute, Department of Agriculture pp 49-54.
Chankaew S, Somta P, Sorajjapinun W, Srinives P (2011). Quantitative trait loci mapping of Cercospora leaf spot resistance in mungbean, Vigna radiata (L.) Wilczek. Molecular Breeding 28:255-264. https://doi.org/10.1007/s11032-010-9478
Chueakhunthod W, Ngampongsai S, Jinagool W, Tantasawat PA (2018). Genetic diversity of mungbean and blackgram based on EST-SSR analysis. International Journal of Advances in Science, Engineering and Technology 6:78-82. http://ijaseat.iraj.in/paper_detail.php?
Curtis PE, Ogreen WL, Hagmen RH (1969). Varietal effect in soybean photosynthesis and photorespiration. Crop Science 10:42-45. https://doi.org/10.2135/cropsci1969.0011183X000900030021x
Das RT, Barua PK (2015). Association studies for yield and its components in green gram. International Journal of Agriculture Environment and Biotechnology 8:561-565. https://doi.org/10.5958/2230-732X.2015.00086.8
Espósito MA, Milanesi LA, Martin E, Cointry E (2009). Comparison of morphological and molecular data for pea (Pisum sativum) in low and high yielding environments. New Zealand Journal of Crop and Horticultural Science 37:227-233. https://doi.org/10.1080/01140670909510268
Ferriol M, Picó B, Nuez F (2003). Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP. Theoretical and Applied Genetics 107:271-282. https://doi.org/10.1007/s00122-003-1242-z
Gao J, Seneweera S, Li P, Zong YZ, Dong Q, Lin E, Hao X (2015). Leaf photosynthesis and yield components of mung bean under fully open-air elevated [CO2]. Journal of Integrative Agriculture 14:977-983. https://doi.org/10.1016/S2095-3119(14)60941-2
García-Martínez S, Andreani L, Garcia-Gusano M, Geuna F, Ruiz JJ (2006). Evaluation of amplified fragment length polymorphism and simple sequence repeats for tomato germplasm fingerprinting: utility for grouping closely related traditional cultivars. Genome 49:648-656. https://doi.org/10.1139/g06-016
Garg GK, Verma PK, Kesh H (2017). Genetic variability, correlation and path analysis in mungbean [Vigna radiata (L.) Wilczek]. International Journal of Current Microbiology and Applied Sciences 6:2166-2173. https://doi.org/10.20546/ijcmas.2017.611.255
Hakim L (2008). Variability and correlation of agronomic characters of mungbean germplasm and their utilization for variety improvement program. Indonesian Journal of Agricultural Science 9:24-28. https://doi.org/10.21082/ijas.v9n1.2008.p24-28
Hemavathy AT, Shunmugavalli N, Anand G (2015). Genetic variability, correlation and path co-efficient studies on yield and its components in mungbean [Vigna radiata (L.) Wilezek]. Legume Research 38:442-446. https://doi.org/10.5958/0976-0571.2015.00050.8
Hossain MA, Hamid A, Khaliq MA (2009). Evaluation of mungbean (Vigna radiata (L.) Wilczek) genotypes on the basis of photosynthesis and dry matter accumulation. Journal of Agriculture & Rural Development 7:1-8. https://doi.org/10.3329/jard.v7i1.4415
IBPGR Secretariat (1980). Descriptor for mung bean. AGP:IBPGR/80/35, Rome pp 1-15.
Islam MT, Agata W, Kubota F, Hamid A (1994). Evaluation of characteristics related to photosynthetic rate and leaf area production in mungbean genotypes. Japanese Journal of Crop Science 63:502-509.
Islam MT, Razzaque AHM (2010). Relationships of photosynthetic and related parameters and yield of summer mungbean varieties/mutants. International Journal of Sustainable Crop Production 5:11-15. https://docplayer.net/145082033-Relationships-of-photosynthetic-related-parameters-and-yield-of-summer-mungbean-varieties-mutants.html
Khan MA, Ammar MH, Migdadi HM, El-Harty EH, Alfaifi SA, Farooq M, Alghamdi SS (2016). Field performance and genetic diversity of chickpea genotypes. International Journal of Agriculture and Biology 18:683-688. https://doi.org/10.17957/IJAB/15.0151
Khajudparn P (2009). Characters associated with yield potential and development of molecular markers for powdery mildew resistance in mungbean. PhD Thesis, Suranaree University of Technology, Nakhon Ratchasima.
Khajudparn P, Tantasawat P (2011). Relationships and variability of agronomic and physiological characters in mungbean. African Journal of Biotechnology 10:9992-10000. https://doi.org/10.5897/AJB11.1288
Khajudparn P, Wongkaew S, Thipyapong P (2007). Mungbean powdery mildew resistance. Identification of genes for powdery mildew resistance in mungbean. In: Proceeding of the 8th African Crop Science Conference. El-Minia, Egypt pp 743-745.
Khattak GSS, Srinives P, Kim DH (1995). Yield partitioning in high yielding mungbean (Vigna radiata (L.) Wilczek). Kasetsart Journal: Natural Science 29:494-497. https://www.lib.ku.ac.th/KUJN/TAB451304.pdf
Kumar A, Sharma NK, Kumar R, Sanadya SK, Sahoo S (2018). Correlation and path analysis for seed yield and components traits in mungbean under arid environment. International Journal of Chemical Studies 6:1679-1681.
Lapointe FJ, Legendre P (1992). Statistical significance of the matrix correlation coefficient for comparing independent phylogenetic trees. Systematic Biology 41:378-384. https://doi.org/10.1093/sysbio/41.3.378
Li G, Quiros CF (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics 103:455-461. https://doi.org/10.1007/s001220100570
Lodhi MA, Ye GN, Weeden NF, Reisch BI (1994). A simple and efﬁcient method for DNA extraction from grapevine cultivars and Vitis species. Plant Molecular Biology Reporter 12:6-13. https://doi.org/10.1007/BF02668658
Long SP, Zhu XG, Naidu SL, Ort DR (2006). Can improvement in photosynthesis increase crop yields? Plant, Cell & Environment 29:315-330. https://doi.org/10.1111/j.1365-3040.2005.01493.x
Manivelan K, Karthikeyan M, Blessy V, Priyanka AR, Palaniyappan S, Thangavel P (2019). Studies on correlation and path analysis for yield and yield related traits in greengram [Vigna radiata (L.) Wilczek]. The Pharma Innovation Journal 8:165-167. https://www.thepharmajournal.com/archives/ 2019/vol8issue9/PartD/8-9-35-920.pdf
Mantel N (1967). The detection of disease clustering and a generalized regression approach. Cancer Research 27:209-220. https://cancerres.aacrjournals.org/content/27/2_Part_1/209
Marappa N (2008). Screening of mungbean genotypes and its wild relatives for resistant sources to Cercospora leaf spot disease. Asian Journal of Biological Sciences 3:324-326.
Mondal MMA, Hakim MA, Juraimi Abdul Shukor, Azad MKA, Karim MR (2011). Contribution of morpho-physiological attributes in determining the yield of mungbean. African Journal of Biotechnology 10:12897-12904. https://doi.org/10.5897/AJB11.373
Nagl N, Ajdukovic KT, Barac G, Baburski A, Seccareccia I, Milic D, Katic S (2011). Estimation of genetic diversity in tetraploid alfalfa populations based on RAPD markers for breeding purposes. International Journal Molecular Sciences 12:5449-5460. https://doi.org/10.3390/ijms12085449
Poczai P, Verga I, Laos M, Cseh A, Bell N, Valkonen JPT, Hyvonen J (2013). Advances in plant gene-targeted and functional markers: a review. Plant Methods 9:6-36. https://doi.org/10.1186/1746-4811-9-6
Poolsawat O, Kativat C, Arsakit K, Tantasawat PA (2017). Identification of quantitative trait loci associated with powdery mildew resistance in mungbean using ISSR and ISSR-RGA markers. Molecular Breeding 37:150-161. https://doi.org/10.1007/s11032-017-0753-2
Rhodes I (1972). Yield, leaf area index and photosynthetic rate in some perennial rye grass (Lolium perene L.) selection. The Journal of Agricultural Science 78:509-511. https://doi.org/10.1017/S002185960002 6460
Sambrook J, Russel DW (2001). Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press (3rd edn), Cold Spring Harbor, New York, NY, USA.
Shao QS, Guo QS, Deng YM, Guo HP (2010). A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers. Biochemical Systematics and Ecology 38:1160-1169. https://doi.org/10.1016/j.bse.2010.11.002
Smith JSC, Smith OS (1989). The description and assessment of distances between inbred lines of maize. II. The utility of morphological, biochemical and genetic descriptors and a scheme for testing of distinctiveness between inbred lines. Maydica 34:151-161.
Somta P, Srinives P (2007). Genome research in mungbean (Vigna radiata (L.) Wilczek) and blackgram (V. mungo (L.) Hepper). Science Asia 33:69-74. https://doi.org/10.2306/scienceasia1513-1874.2007.33 (s1).069
Somta P, Sommanas W, Srinives P (2009). Molecular diversity assessment of AVRDC-the world vegetable center elite-parental mungbeans. Breeding Science 59:149-157. https://doi.org/10.1270/jsbbs.59.14 9
Srinivasan PS, Chandrababu R, Natarajratnam N, Rangaswamy SRS (1985). Leaf photosynthesis and yield potential in green gram (Vigna radiata (L.) Wilczek) cultivars. Tropical Agriculture (Trinidad) 62:222-224.
Tahir A, Ilyas MK, Sardar MM, Pouya AK, Rasouli F, Bibi A, … Ghafoor A (2020). Selection criteria for yield potential in a large collection of Vigna radiata (L.) accessions. Euphytica 216:138-149. https://doi.org/10.1007/s10681-020-02675-x
Tantasawat P, Trongchuen J, Prajongjai T, Thongpae J, Petkhum C, Seehalak W, Machikowa, T (2010). Variety identification and genetic relationships of mungbean and blackgram in Thailand based on morphological characters and ISSR analysis. African Journal of Biotechnology 9:4452-4464. https://doi.org/10.5897/AJB09.1768
Tantasawat PA, Poolsawat O, Arsakit K, Papan P (2020). Identification of ISSR, ISSR-RGA and SSR markers associated with Cercospora leaf spot resistance gene in mungbean. International Journal of Agriculture and Biology 23:447‒453. https://doi.org/10.17957/IJAB/15.1308
Yimram T, Somta P, Srinives P (2009). Genetic variation in cultivated mungbean germplasm and its implication in breeding for high yield. Field Crops Research 112:260-266. https://doi.org/10.1016/j. fc r.2009.03.013
Copyright (c) 2020 Notulae Botanicae Horti Agrobotanici Cluj-Napoca
This work is licensed under a Creative Commons Attribution 4.0 International License.
Open Access Journal:
The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.