Genetic relationship of mungbean and blackgram genotypes based on agronomic and photosynthetic performance and SRAP markers

Witsarut  CHUEAKHUNTHOD; Wanploy  JINAGOOL; Kantima  MEECHAROEN; Rungrapee  KHWANMAN; Pattaradol  PATTANARAM; Natchaya  JANTARAT; Pratthana  PALAPHON; Sumana  NGAMPONGSAI; Piyada A. TANTASAWAT

doi:10.15835/nbha48412114

Witsarut CHUEAKHUNTHOD Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (TH)
Wanploy JINAGOOL Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (TH)
Kantima MEECHAROEN Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (TH)
Rungrapee KHWANMAN Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (TH)
Pattaradol PATTANARAM Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (TH)
Natchaya JANTARAT Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (TH)
Pratthana PALAPHON Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (TH)
Sumana NGAMPONGSAI Field and Renewable Energy Crops Research Institute, 50 Phahonyothin Avenue, Chatuchak District, Bangkok 10900 (TH)
Piyada A. TANTASAWAT Suranaree University of Technology, School of Crop Production Technology, Institute of Agricultural Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000 (TH)

DOI: https://doi.org/10.15835/nbha48412114

Keywords: agronomic characters; cluster analysis; genetic diversity; photosynthesis; sequence-related amplified polymorphism; Vigna mungo (L.) Hepper; Vigna radiata (L.) Wilczek

Abstract

Genetic identification is at the base of parental selection in varietal development programs. Agronomic and photosynthetic characters and sequence-related amplified polymorphism (SRAP) markers were analyzed for two legume species which included 23 mungbean (Vigna radiata (L.) Wilczek) and four blackgram (Vigna mungo (L.) Hepper) genotypes. The results revealed that the seeds/pod, plant height, pods/plant, pod length, days to flowering, and all photosynthetic characters studied had a significant correlation with the yield/plant. Using UPGMA analysis with phenotypic data, five clusters and two individuals were identified. Twenty-five SRAP primer combinations generated 562 amplified bands, of which 507 were polymorphic (90.2%). The average numbers of scorable and polymorphic bands/primer pair were 22 and 20, respectively. Two major clusters coincided with two species having a 100% bootstrap value. Within the mungbean cluster, there were two subclusters containing 12 and 11 mungbean genotypes. Mantel’s test demonstrated that the polymorphisms given by SRAPs were associated with agronomic and photosynthetic variability (r = 0.734, p < 0.01). These results allow promising mungbean genotypes to be identified through genetic diversity and field performance which can be utilized as potential parents towards future breeding programs. Moreover, the factors which contribute most to yield/plant can be simultaneously used as selection criteria for yield improvement.

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