Effects of exogenous NO on antioxidant system of Taxus plants under simulated acid rain stress

Bing SUN; Mengxi  LI; Die  HU; Xiao  PAN; Yongjun  FEI

doi:10.15835/nbha49212052

Authors

Bing SUN Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei Province 434025 (CN)
Mengxi LI Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei Province 434025 (CN)
Die HU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei Province 434025 (CN)
Xiao PAN BAOWU GROUP Wuhan WISCO Green City Technology Development CO., LTD, WuHan, Hubei Province430083 (CN)
Yongjun FEI Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei Province 434025 (CN)

DOI:

https://doi.org/10.15835/nbha49212052

Keywords:

acid rain, antioxidant system, nitric oxide (NO), sodium nitroprusside (SNP), Taxus plants

Abstract

Taxus is a famous medicinal and landscape tree species. The aim of this study was to explore the effect of exogenous nitric oxide (NO) on the resistance of Taxus plants to acid rain stress and to identify Taxus species with strong acid rain resistance by principal component analysis and comprehensive evaluation. In this study, sodium nitroprusside (SNP) was used as the exogenous NO donor. The effects of different SNP solution concentrations on the antioxidant systems of three Taxus species subjected to simulated acid rain stress (pH = 3.0) were compared. In order to achieve this goal, we determined the rate of O^2- production, the ASA and GSH contents in leaves of three Taxus plants (Taxus mairei, Taxus chinensis, and Taxus yunnanensis). At the same time, the active leaves of some antioxidant enzymes (SOD, POD, CAT, APX and GR) were determined. For Taxus chinensis plants subjected to acid rain stress, treatment with an SNP concentration of 0.25 mmol·L^-1 led to the most significant improvements in the antioxidant system. For Taxus mairei and Taxus yunnanensis, the treatment with the SNP concentration of 0.50 mmol·L^-1 was best for improving their antioxidant systems under stress. Meanwhile, Taxus chinensis had the strongest resistance to simulated acid rain, followed by Taxus mairei and Taxus yunnanensis.

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