Biochemical profile, antioxidant capacity and allelopathic effects from five Ziziphyus spina-christi (L.) provenances growing wild in Saudi Arabia


  • Souheila NAGHMOUCHI University of Tunis, National Research Institute of Rural Engineering, Water, and Forestry, Carthage, Street of Hedi Karay BP. N°10 Ariana 2080 (TN)
  • Moodi ALSUBEIE Imam Mohammed Ibn Saud Islamic University, College of Sciences, Biology Department, Riyadh (SA)



allelopathic effects; antioxidant activity; provenance; Saudi Arabia; secondary metabolites; Ziziphyus spina-christi


The aim of this work was to evaluate phenolic profile, antioxidant capacity and allelopathic effects of six Ziziphyus spina-christi provenances in Saudi Arabia. Total phenols, flavonoids, tannins and antioxidant activity were evaluated. Total phenols, condensed tannins and total flavonoids were present at levels of 62.29 mg GAE/g DM, 16.69 mg CE/g DM and 15.45 µg mg QE/g DM, respectively. The high antioxidant activity (0.15 mg/ml) was noted in Mecca Road provenance. The highest germination, shoots and radicle lengths of tested species were observed in At-taif provenance. Z. spina-christi leaf extracts may be suggested in foods and pharmaceutical industries. Leaf extracts could also provide a natural herbicide with a positive impact on the environment. Provenance of Jizan, Northern Borders and Mecca Road were shown to be particularly effective in antioxidant capacity, while Provenance of Riyadh and Northern Borders shown to have the best allelopathic activity.


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Abdel-Zaher AO, Salim SY, Assaf MH, Abdel-Hady RH (2014). Effect of silver nanoparticles on rice (Oryza sativa L. cv. KDML 105) seed germination and seedling growth. Ecotoxicology and Environmental Safety 104:302-309.

Abdoul-Azize S (2016). Potential benefits of jujube (Zizyphus lotus L.) bioactive compounds for nutrition and health. Journal of Nutrition and Metabolism

Adzu B, Haruna AK (2007). Studies on the use of Zizyphus spina-christi against pain in rats and mice. African Journal of Biotechnology 6:1317-1324.

Akrout A, Gonzalez LA, El Jani H, Madrid PC (2011). Antioxidant and antitumor activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia. Food and Chemical Toxicology 49(2):342-347.

Alansi S, Tarroum M, Al-Qurainy F, Khan S, Nadeem M (2016). Use of ISSR markers to assess the genetic diversity in wild medicinal Ziziphyus spina-christi (L.) Willd. collected from different regions of Saudi Arabia. Biotechnology & Biotechnological Equipment 30(5):942-947.

Alghazeer HE, Saleh N, Al-Najjar A, Hebail F (2012). Antioxidant and antimicrobial properties of five medicinal Libyan plants extracts. Natural Science 4(5):324-335.

Al-Hassan HO (2006). Wild plants of the northern region of the kingdom of Saudi Arabia (field guide with photographs). Ministry of Agriculture, Camel and Range Research Center, Al-Jouf, Saudi Arabia.

Alqarni A, Hassan A, Owayss A (2015). Evaluation of Acacia gerrardii Benth. (Fabaceae: Mimosoideae) as a honey plant under extremely hot-dry conditions: Flowering phenology, nectar yield and honey potentiality. Journal of Animal and Plant Sciences 25(6):1667-1674.

Alqarni AS (2015). Honeybee foraging, nectar secretion, and honey potential of wild jujube trees, Ziziphyus nummularia. Neotropical Entomology 44(3):232-241.

Andarwulan N, Batari R, Sandrosari DA, Bolling B, Wijaya H (2010). Flavonoid content and antioxidant activity of vegetable from Indonesian. Food Chemistry 121(4):1231-1235.

Arora K, Batish DR, Singh HP, Kohli RK (2015). Allelopathic potential of the essential oil of wild marigold (Tagetes minuta L.) against some invasive weeds. Journal of Environmental and Agricultural Sciences 3:56-60.

Asharafi ZY, Sadeghi S, Mashhadi HR, Alizade HM (2008). Study of allelopathical effects of barley on inhibition of germination and growth of seeding green foxtail. An Open Access Journal published by ICRISA 6:1-6

Ashraf A, Sarfraz RA, Anwar F, Shahid SA, Alkharfy KM (2015). Chemical composition and biological activities of leaves of Ziziphyus mauritiana L. native to Pakistan. Pakistan Journal of Botany 47:367-376.

Azim UM (2008). Allelopathy: Advances, challenges and opportunities. Allelopathy in Sustainable Agriculture and Forestry pp 25-38.

Badr SF, El-Sherif NA, Shabana I, El-Deeb M, AL-Shinqiti FYM (2020). Evaluation of genetic variation, antioxidant and antibacterial activities of two Sidr varieties in Medinadoi. Pakistan Journal of Pharmaceutical Sciences 33(1):61-69.

Basuny AM, Shaker MA, Hoda AF (2013). Utilization from fruits and leaves of Napek (Zizyphus spina-christi) as a source of bioactive components. International Journal of Chemical and Natural Science 1:29-36.

Bettaieb A, Moujahed N, Ksouri R (2012). Secondary coumpounds characterization in some autochthonous species from a Northern-Eastern region of Tunisia. Options Méditerranéennes. Série A, Séminaires Méditerranéens 102:371-374.

Bhadoria PPS (2011). Allelopathy: A natural way towards weed management. American Journal of Experimental Agriculture 1:7-20.

Brito SMO, Coutinho HDM, Talvani A, Coronel C, Barbosa AGR, Vegad C, … Menezes IRA (2015). Analysis of bioactivities and chemical composition of Ziziphyus joazeiro Mart. using HPLC-DAD. Food Chemistry 186:185-191.

Bukar M, Kyari MZ, Gwaski PA, Gudusu M, Kuburi FS, Abadam YI (2015). Evaluation of phytochemical and potential antibacterial activity of Ziziphyus spina-christi L. against some medically important pathogenic bacteria obtained from University of Maiduguri Teaching Hospital, Maiduguri, Borno State – Nigeria. Journal of Pharmacognosy and Phytochemistry 3:98-101.

Chaudhary SA (1999-2001). Flora of the Kingdom of the Saudi Arabia, vols. 1-3, Riyadh. Ministry of Agriculture and Water press.

Chaudhary SA (2000). In: Flora of the Kingdom of the Saudi Arabia, vol. II. Ministry of Agriculture and Water, Riyadh, Saudi Arabia.

Chaudhary SA (2001). Flora of the Kingdom of Saudi Arabia (Illustrated). Riyadh. National Agriculture and Water Research Center.

Chaudhary SA (2001). In: Flora of the Kingdom of the Saudi Arabia, vol. III. Ministry of Agriculture and Water, Riyadh, Saudi Arabia.

Chaudhary SA, Akram M (1987). Weeds of Saudi Arabia and the Arabian Peninsula. Regional Agriculture and Water Research Center, Ministry of Agriculture and Water, Riyadh, Saudi Arabia.

Chaudhary SA, Al-Jowaid AA (1999). Vegetation of the Kingdom of Saudi Arabia. National Agriculture and Water Research Center. Ministry of Agriculture and Water. Riyadh, Saudi Arabia.

Collenette S (1999). Wild Flowers of Saudi Arabia, Riyadh, National Commission for Wild Life Conservation and Development (VCWCD), pp 779.

Cope T (1985). A key to the grasses of Arabian Peninsula (Studies in the Flora of Arabia XV). Arabian Journal Science Research, Special Publication.

Dafni A, Levy S, Lev E (2005). The ethnobotany of Christ’s Thorn Jujube (Ziziphus spina-christi) in Israel. Journal of ethnobiology and ethnomedicine 1(1):8.

Dewanto V, Wu X, Adom K, Liu RH (2002). Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Agriculture and Food Chemistry 50(10):3010-3014.

Earp CF, Akingbala JO, Ring SH, Rooney LW (1981). Evaluation of several methods to determine tannins in sorghums with varying kernel characteristics. Cereal Chemistry 58:234-248.

Ebrahimi NS, Hadian J, Mirjalili MH, Sonboli A, Yousefzadi M (2008). Essential oil composition and antibacterial activity of Thymus caramanicus at different phenological stages. Food Chemistry 110:927-931.

Elaloui M, Ennajah A, Ghazghazi H, Ben YI, Ben ON, Hajlaoui MR, … Laamouri A (2017). Quantification of total phenols, flavonoides and tannins from Ziziphyus jujuba (mill.) and Ziziphyus lotus (l.) (Desf). Leaf extracts and their effects on antioxidant and antibacterial activities. International Journal of Secondary Metabolite 4(1):18-26.

Elaloui M, Ghazghazi H, Ennajah A, Manaa S, Guezmir W, Karray NB, Laamouri A (2016a). Phenolic profile, antioxidant capacity of five Ziziphyus spina-christi (L.) Willd provenances and their allelopathic effects on Trigonella foenum-graecum L. and Lens culinaris L. seeds. Natural Product Research 31(10):1209-1213.

Elaloui M, Laamouri A, Ennajah A, Cerny M, Mathieu C, Vilarem G, … Hasnaoui B (2016c). Phytoconstituents of leaf extracts of Ziziphyus jujuba Mill. plants harvested in Tunisia. Industrial Crops and Product 83:133-139.

Elaloui M, Soltani I, Laamouri A, Ennajah A, Houcine S (2016b). Allelopathic activity of extracts from leaves of Ziziphyus spina-christi collected in fie Tunisian ecotypes. International Journal of Innovation Science and Research 20:333-342.

El-Ansary A, Warsy A, Daghestani M, Merghani NM, Al-Dbass A, Bukhari W, … Shafi BR (2018). Characterization, antibacterial, and neurotoxic effect of green synthesized nanosilver using Ziziphyus spina Christi aqueous leaf extract collected from Riyadh, Saudi Arabia. Materials Research Express 5:25-33.

Farooqi A (1997). Plants of the Qur’an (Lucknow, India). Sidrah Publishers) pp 65-74.

Habib HM, AlMeqbali FT, Kamal H, Souka UD, Ibrahim WH (2014). Bioactive components, antioxidant and DNA damage inhibitory activities of honeys from arid regions. Food Chemistry 153:28-34.

Hadacek F (2002). Secondary metabolites as plant traits: current assessment and future perspectives. Critical Reviews in Plant Sciences 21(4):273-322.

Hammi KM, Jdey A, Abdelly Ch, Majdoub H, Ksouri R (2015). Optimization of ultra sound assisted extraction of antioxidant compounds from Tunisian Zizyphus lotus fruits using response surface methodology. Food Chemistry 184:80-89.

Hashempour A, Ghazvini, RF, Bakhshi D, Sanam SA (2010). Fatty acids composition and pigments changing of virgin olive oil (Olea europea L.) in five cultivars grown in Iran. Australian Journal of Crop Science 4(4):258-263.

Islam MS, Yoshimot OM, Ishigure K, Okuno S, Yamakawa O (2003). Effect of artificial shading and temperature on radical scavenging activity and polyphenolic composition in sweet potato (Ipomoea batatas L.) leaves. Journal American Society Horticultural Science 128:182-187.

Itidel C, Chokri M, Mohamed B, Yosr Z (2013). Antioxidant activity, total phenolic and flavonoid content variation among Tunisian natural populations of Rhus tripartita (Ucria) Grande and Rhus pentaphylla Desf. Industrial Crops and Products 51:171-177.

Jiang JG, Huang XJ, Chen J, Lin QS (2007). Comparison of the sedative and hypnotic effects of flavonoids, saponins, and polysaccharides extracted from Semen Ziziphyus jujube. Natural Product Research 21:310-320.

Kato-Noguchi H, Macaias FA (2006). Possibile mecanisme of inhibition of 6-methoxy-benzoxazoline-2(3H)-one on germination of cress (Lepidium sativum L.). Journal of Chemical Ecology 32:1101-1109.

Lee KW, KimYJ, Lee HJ, Lee CY (2003). Cocao has more phenolic phytochemicals and a higher antioxidant capacity than teas and red wine. Food Chemistry 51:7292-7295.

Li J W, Ding S D and Ding X L (2005). Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube. Process Biochemistry 40:3607-3612.

Medini F, Fellah H, Ksouri R, Abdelly C (2014). Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum extremophile. Journal of Taibah University for Science 8:216-224.

Migahid AM (1996). Flora of Saudi Arabia, vols. I-III. Jeddah: King Abdul Aziz University Press.

Miliauskas G, Venskutonis PR, Van Beek TA (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extract. Food Chemistry 85:231-233.

Mizrahi Y, Nerd A, Sitrit Y (2002). New fruits for arid climates. In: Janick J, Whipkey A (Eds). Trends in new crops and new uses. Alexandria (VA): ASHS Press pp 378-384.

Owayss AA, Elbanna K, Iqbal J, Abulreesh HH, Organji SR, Raweh HSA, Alqarni AS (2020). In vitro antimicrobial activities of Saudi honeys originating from Ziziphyus spina-christi L. and Acacia gerrardii Benth. trees. Food Science & Nutrition 8:390-401.

Patil RB, Kore BA (2017). Phytoconstituents, pigments, gas chromatography mass

spectrometry analysis, and allelopathy effect of alternanthera ficoidea (L.) P. Beauv. Asian Journal of Pharmaceutical and Clinical Research 10:103-108.

Popova M, Bankova V, Butovska D, Petkov V, Nikolova‐Damyanova B, Sabatini AG, ... Bogdanov S (2004). Validated methods for the quantification of biologically active constituents of poplar‐type propolis. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques 15(4):235-240.

Prasad R, Tripathi VD, Singh P, Handa AK, Alam B, Singh R, Chaturvedi OP (2016). Allelopathic potential of Butea monosperma L.: Effect of aqueous leaf extract on seed germination and seedling growth of winter season (rabi) crops. Indian Journal of Agroforestry 18:63-69.

Reigosa M, Gomes AS, Ferreira AG, Borghetti F (2013). Allelopathic research in Brazil. Acta Botanica Brasillica 27(4):629-646.

Rice-Evans CA, Miller NJ, Paganga G (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine 20:933-956.

Rouz S, Ben Farhat M, Gammar-Ghrabi Z (2015). The aqueous extract effect of six species on the chicory adventitious of the berseem fields in Tunisia. Journal of New Sciences, Agriculture and Biotechnology 20(4):798-803.

Saanchez-Moreiras AM, Coba de la Pena T, Reigosa MJ (2008). The natural compound benzoxazolin-2(3H) one selectively retards cell cycle in lettuce root meristems. Phytochemistry 69:2172-2179.

Seif Eldin AM, Ahmed SK, Walid SK, Azim MK (2015). Antigiardial activity of glycoproteins and glycopeptides from Ziziphyus honey. Natural Product and Research 22:2100-2102.

Singleton VL, Orthofer R, Lamuela-Raventos RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent. Methods in Enzymology 299:152-178.

Tim TPC, Lamb AJ (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents 26:343-356.

Tlili H, Hanen N, Ben Arfa A, Neffati M, Boubakri A, Buonocore D (2019). Biochemical profile and in vitro biological activities of extracts from seven folk medicinal. Plants growing wild in southern Tunisia. PLoS One 14(9):3021-3049.

Turk M, Shatnawi AMK, Tawaha A M (2002). Inhibitory effects of aqueous extracts of black mustard on germination and growth of alfalfa. Weed Biology and Management 3:37-40.

Turk MA, Tawaha AM (2002). Inhibitory effects of aqueous extract of barely on germination and growth of lentil. Pakistan journal of Agronomy 1:28-30.

Turker M, Bttal P, Agar G, Guluce M, Sahin F, Yildirim N (2008). Allelopathic effects of plants extracts on physiological process during maize seed germination. Allelopathy Journal 21:273-286.

Waggas AM, Al-Hasani RH (2010). Neurophysiological study on possible protective and therapeutic effects of Sidr (Zizyphus spina-christi L.) leaf extract in male albino rats treated with pentylenetetrazol. Saudi Journal of Biological Sciences 17:269-274.




How to Cite

NAGHMOUCHI, S., & ALSUBEIE, M. (2020). Biochemical profile, antioxidant capacity and allelopathic effects from five Ziziphyus spina-christi (L.) provenances growing wild in Saudi Arabia. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(3), 1600–1612.



Research Articles
DOI: 10.15835/nbha48312025