Effect of intercropping alfalfa on physiological and biochemical parameters of young grapevine plants cultivated on agricultural and contaminated soils

  • Sabrine JEDER Laboratory of Legumes, Centre of Biotechnology of Borj-Cedria, B.P. 901, 2050 Hammam-Lif
  • Issam NOUAIRI Laboratory of Legumes, Centre of Biotechnology of Borj-Cedria, B.P. 901, 2050 Hammam-Lif
  • Fadwa MELKI Laboratory of Legumes, Centre of Biotechnology of Borj-Cedria, B.P. 901, 2050 Hammam-Lif
  • Samir CHEBIL University of Tunis El Manar, Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj-Cedria, B.P. 901, 2050 Hammam-Lif
  • Faten LOUATI Laboratory of Legumes, Centre of Biotechnology of Borj-Cedria, B.P. 901, 2050 Hammam-Lif
  • Haythem MHADHBI Laboratory of Legumes, Centre of Biotechnology of Borj-Cedria, B.P. 901, 2050 Hammam-Lif
  • Kais ZRIBI Laboratory of Legumes, Centre of Biotechnology of Borj-Cedria, B.P. 901, 2050 Hammam-Lif
Keywords: antioxidant enzyme; grapevine; growth; heavy metals; intercropping; Medicago sativa

Abstract

Our research aimed to reveal the capacity of intercropping with Medicago sativa-rhizobia in the amelioration of grapevine growth in agricultural and a Cd/Pb contaminated soils. A local variety of grapevine was cultivated in monocropping and in intercropping with Medicago sativa inoculated or not with its associated rhizobia. Intercropping with alfalfa induced a significant increase in shoot and root biomass of grapevine in the agricultural soil. However, in the contaminated soil, a slight increase in root biomass was observed. Concerning photosynthesis apparatus, we showed that the presence of Cd and Pb in the soil induced a significant decrease in both CO2 assimilation rate and stomatal conductance. Interestingly, intercropping with alfalfa only and with rhizobia alleviate this effect. Similar results are obtained for chlorophyll and carotenoid content. This was associated with a significant decrease in the malondialdehyde level in leaves and roots of grapevine cultivated in intercropping with alfalfa with and without inoculation in the two soils as compared the monoculture treatment. Comparison between treatments revealed also that intercropping with alfalfa induced a decrease in the activities of some enzymes implicated in the defence to the oxidative stress such as catalase and superoxide dismutase. Regarding soluble protein content, it is needed to signal the improvement of this parameter with the intercropping system in the contaminated soil when compared to the monocropping treatment. This work highlights the importance of the use of legumes in intercropping with grapevine as intercrop plant non-competitive for soil nutrient and proving N supply for associated plants.          

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References

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Published
2021-02-15
How to Cite
JEDER, S., NOUAIRI, I., MELKI, F., CHEBIL, S., LOUATI, F., MHADHBI, H., & ZRIBI, K. (2021). Effect of intercropping alfalfa on physiological and biochemical parameters of young grapevine plants cultivated on agricultural and contaminated soils. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12017. https://doi.org/10.15835/nbha49112017
Section
Research Articles
CITATION
DOI: 10.15835/nbha49112017