Influence of pulsed electromagnetic field as a pre-sowing treatment on germination, plant growth and yield of broad beans

  • Nikolaos KATSENIOS Agricultural University of Athens, Department of Crop Science, Laboratory of Agronomy, 75 Iera Odos Str., 11855 Athens
  • Panagiotis SPARANGIS Institute of Soil and Water Resources, Department of Soil Science of Athens, Hellenic Agricultural Organization - Demeter, Sofokli Venizelou 1, Lycovrissi, 14123, Attica
  • Ioanna KAKABOUKI Agricultural University of Athens, Department of Crop Science, Laboratory of Agronomy, 75 Iera Odos Str., 11855 Athens
  • Aspasia EFTHIMIADOU Institute of Soil and Water Resources, Department of Soil Science of Athens, Hellenic Agricultural Organization - Demeter, Sofokli Venizelou 1, Lycovrissi, 14123, Attica,
Keywords: broad beans; forage; pre-sowing; pulsed electromagnetic field; seed priming


Magnetic field is a variable abiotic factor, like temperature, air, soil and water that interacts with the living organisms. The use of different types of magnetic field at many different plant species is increasing and the results are promising. A two-year field experiment was established following a completely randomized design, to investigate the effect of pre-sowing magnetic field treatment for 0, 15, 30 and 45 min on three broad bean varieties. The obtained results showed that the treatment of seeds with pulsed electromagnetic field increased germination percentage, chlorophyll content, leaf area, photosynthetic rate, transpiration rate, stomatal conductance and dry weight. In general, the exposure of seeds for 15 min, gave the highest values compared to the control treatments for the three varieties used. A very interesting result is the fact that although there was an influence of the exposure of magnetic field on the dry weight of the plants, there was not a positive or a negative influence on the seed yield for both years. This means that magnetic field enhanced the vegetative development of the broad beans, but it had no effect on the reproductive development. The results indicate that magnetic field can be used in the cases that the productive direction of the cultivation of broad beans is for livestock forage and silage, as the pre-sowing treatment of seeds had a positive effect on plant dry weight.


In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. The article is to be paginated when the complete issue will be ready for publishing (Volume 48, Issue 3, 2020). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.


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Aguilar CH, Pacheco AD, Carballo AC, Orea AC, Ivanov R, Bonilla JLL, Montanez JPV (2009). Alternative magnetic field irradiation effects on three genotype maize seed field performance. Acta Agrophysica 14(1): 7-17.,107313,0,2.html

Aksyonov SI, Bulychev AA, Grunina TYu, Goryachev SN, Turovetsky VB (2001). Effects of ELF-EMF treatment on wheat seeds at different stages of germination and possible mechanisms of their origin. Electromagnetic Biology and Medicine 20(2):231-253.

Aladjadjiyan A, Ylieva T (2003). Influence of stationary magnetic field on the early stages of the development of tobacco seeds (Nicotiana tabacum L.). Journal of Central European Agriculture 4(2):131-138.

Aladjadjiyan A (2012). Physical factors for plant growth stimulation improve food quality. Food Production-Approaches, Challenges and Tasks 270.

Ashraf M, Foolad MR (2005). Pre-sowing seed treatment – A shotgun approach to improve germination, plant growth and crop yield under saline and non-saline conditions. Advances in Agronomy 88:223-271.

Bhardwaj J, Anand A, Nagarajan S (2012). Biochemical and biophysical changes associated with magneto-priming in germinating cucumber seeds. Plant Physiology and Biochemistry 57:67-73.

Bilalis D, Katsenios N, Efthimiadou A, Karkanis A (2012a). Pulsed electromagnetic fields effect in oregano rooting and vegetative propagation: A potential new organic method. Acta Agriculturae Scandinavica, Section B - Soil & Plant Science 62:94-99.

Bilalis D, Katsenios N, Efthimiadou A, Karkanis A (2012b). Investigation of pulsed electromagnetic field as a novel organic pre-sowing method on germination and initial growth stages of cotton. Electromagnetic Biology and Medicine 31(2):143-150.

Bilalis D, Katsenios N, Efthimiadou A, Karkanis A, Khah ME, Mitsis T (2013). Magnetic field pre-sowing treatment as an organic friendly technique to promote plant growth and chemical elements accumulation in early stages of cotton. Australian Journal of Crop Science 7(1):46-50.

Carbonell MV, Martínez E, Amaya JM (2000). Stimulation of germination in rice (Oryza sativa L.) by a static magnetic field. Electromagnetic Biology and Medicine 19 (1):121-128.

De Souza A, García D, Sueiro L, Gilart F, Porras E, Licea L (2006). Pre-sowing magnetic treatments of tomato seeds increase the growth and yield of plants. Bioelectromagnetic s27(4):247-257.

Es’kov EK, Rodionov Yu A (2010). Initial growth processes in seeds in magnetic fields, strengthened or weakened in relation to the geomagnetic field. Biology Bulletin 37:49-55.

Flórez M, Carbonell MV, Martínez E (2007). Exposure of maize seeds to stationary magnetic fields: Effects on germination and early growth. Environmental and Experimental Botany 59(1):68-75.

Hussain MS, Dastgeer G, Afzal AM, Hussain S, Kanwar RR (2020). Eco-friendly magnetic field treatment to enhance wheat yield and seed germination growth. Environmental Nanotechnology, Monitoring & Management 14:100299.

Javed N, Ashraf M, Akram Nudrat A, Al-Qurainy F (2011). Alleviation of adverse effects of drought stress on growth and some potential physiological attributes in maize (Zea mays L.) by seed electromagnetic treatment. Photochemistry and Photobiology 87:1354-1362.

Katsenios N, Bilalis D, Efthimiadou A, Aivalakis G, Nikolopoulou A, Karkanis A, Travlos I (2016). Role of pulsed electromagnetic field on enzyme activity, germination, plant growth and yield of durum wheat. Biocatalysis and Agricultural Biotechnology 6:152-158.

Leelapriya T, Dhilip KS, Sanker Narayan PV (2003). Effect of weak sinusoidal magnetic field on germination and yield of cotton (Gossypium spp.). Electromagnetic Biology and Medicine 22:117-125.

Lichtenthaler HK, Wellburn AR (1983). Determinations of total carotenoids and Chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions11:591-592.

Martínez E, Carbonell MV, Flórez M, Amaya JM, Maqueda R (2009). Germination of tomato seeds (Lycopersicon esculentum L.) under magnetic field. International Agrophysics 23:45-49.,106414,0,2.html

Muszyński S, Gagoś M, Pietruszewski S (2009) Short-term pre-germination exposure to ELF magnetic fields does not Influence seedling growth in durum wheat (Triticum durum). Polish Journal of Environmental Studies 18(6):1065-1072.,88329,0,2.html

Novitsky YuI, Novitskaya GV, Kocheshkova TK, Nechiporenko GA, Dobrovol'skii MV (2001). Growth of green onions in a weak permanent magnetic field. Russian Journal of Plant Physiology 48(6):709-716.

Podlesny J, Pietruszewski S, Podlesna A (2004). Efficiency of the magnetic treatment of broad bean seeds cultivated under experimental plot conditions. International Agrophysics 18:65-71.,106680,0,2.html

Răcuciu M, Creangă D, Horga I (2008). Plant growth under static magnetic field influence. Romanian Journal of Physics 53:331-336.

Radhakrishnan R, Ranjitha Kumari B (2012). Pulsed magnetic field: A contemporary approach offers to enhance plant growth and yield of soybean. Plant Physiology and Biochemistry 59:139-177.

Radhakrishnan R, Ranjitha Kumari B (2013). Influence of pulsed magnetic field on soybean (Glycine max L.) seed germinate seedling growth and soil microbial population. Indian Journal of Biochemistry and Biophysics 50(4):312-317.

Shine M, Guruprasad K, Anand A (2011). Enhancement of germination, growth, and photosynthesis in soybean by pre-treatment of seeds with magnetic field. Bioelectromagnetics 32:474-484.

Soja G, Kunsch B, Gerzabek M, Reichenauer T, Soja A-M,Bolhàr-Nordenkampf HR (2003). Growth and yield of winter wheat (Triticum aestivum L.) and corn (Zea mays L.) near a high voltage transmission line. Bioelectromagnetics 24:91-102.

Vashisth A, Nagarajan S (2008). Exposure of seeds to static magnetic field enhances germination and early growth characteristics in chickpea (Cicer arietinum L.). Bioelectromagnetics 29(7):571-578.

Vashisth A, Nagarajan S (2010). Effect on germination and early growth characteristics in sunflower (Helianthus annuus) seeds exposed to static magnetic field. Journal of Plant Physiology 167(2):149-156.

Vashisth A, Joshi DK (2017). Growth characteristics of maize seeds exposed to magnetic field. Bioelectromagnetics 38(2):151-157.

How to Cite
KATSENIOS, N., SPARANGIS, P., KAKABOUKI, I., & EFTHIMIADOU, A. (2020). Influence of pulsed electromagnetic field as a pre-sowing treatment on germination, plant growth and yield of broad beans. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(3).
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