Responses of physiological activities and lateral root anatomical structure of Cercis glabra to waterlogging stress

  • Jie LUO Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei, 434025
  • Long-Yi YUAN Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei, 434025
Keywords: Cercis glabra Pamp; physiological anatomic structure; waterlogging stress


Cercis glabra is a colour-leaf tree with excellent ornamental value, whereas its physiological and morphological responses to waterlogging stress are still unclear. A potted study was conducted to determine the effects of waterlogging stress on antioxidative enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), lipid peroxidation (in terms of malondialdehyde (MDA) content), relative electric conductivity, and osmotic substance (free proline) of leaves and aerenchyma, lignification, suberization and Casparian strip of lateral roots of C. glabra. The result showed that the SOD, POD, and CAT activity and free proline content of C. glabra were significantly increased by the different degrees of waterlogging stress compared with the non-waterlogged treatment at 8 and 12 days, and the MDA content and relative electric conductivity of C. glabra leaves were significantly increased under the different degrees of waterlogging stress compared to the non-waterlogged treatment at 16 days, and the degrees of change increased among treatments was ranked as total waterlogged > semi-waterlogged > shallow waterlogged. The lateral roots of C. glabra not only formed developed aerenchyma in the cortex but also formed suberization and Casparian strip in the endodermis under semi-waterlogged treatment at 16 days. These results implied that C. glabra had a certain tolerance to waterlogging stress, which was associated with the increasing antioxidant enzyme activity and osmotic adjustment substance content, and with the formation of aerenchyma, suberization and Casparian strip in the lateral root to adapt to the waterlogged environment.



In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. The article is to be paginated when the complete issue will be ready for publishing (Volume 48, Issue 3, 2020). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.


Metrics Loading ...


Chance B, Maehly AC (1955). Assay of catalases and peroxidases. Methods Enzymology 2:764-775.

Di N, Liu Y, Mead D J, Xie Y, Jia L, Xi B (2018). Root-system characteristics of plantation-grown Populus tomentosa adapted to seasonal fluctuation in the groundwater table. Trees 32(1):137-149.

Duhan, S, Kumari A, Lal M, Sheokand S (2019). Oxidative stress and antioxidant defense under combined waterlogging and salinity stresses. reactive oxygen, nitrogen and sulfur species in plants: production, metabolism. Signaling and Defense Mechanisms 113-142.

Ejiri M, Shiono K (2019). Prevention of radial oxygen loss is associated with exodermal suberin along adventitious roots of annual wild species of Echinochloa. Frontiers in Plant Science 10:254.

Esterbauer HK, Cheeseman H (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymology 186:407-421.

Faseela P, Sinisha AK, Thomas TTD, Puthur JT (2018). Oxidative stress and its management in plants during abiotic stress. In metabolic adaptations in plants during abiotic stress. CRC Press pp 111-126.

Fiasconaro ML, Lovato ME, Antolín MC, Clementi LA, Torres N, Gervasio S, Martín CA (2019). Role of proline accumulation on fruit quality of pepper (Capsicum annuum L.) grown with a K-rich compost under drought conditions. Scientia Horticulturae 249:280-288.

Giannopolitis CN, Ries SK (1977). Superoxide dismutase: I. Occurrence in higher plants. Plant Physiology 59:309-314.

Guo YY, Yu HY, Yang MM, Kong D, Zhang YJ (2018). Effect of drought stress on lipid peroxidation, osmotic adjustment and antioxidant enzyme activity of leaves and roots of Lycium ruthenicum Murr. seedling. Russian Journal of Plant Physiology 65:244-250.

Jahromi NSM, Jonoubi P, Majd A, Dehghani M (2019). Root structural changes of two remediator plants as the first defective barrier against industrial pollution, and their hyperaccumulation ability. Environmental Monitoring and Assessment 191:148.

Jia L, Qin X, Lu D, Qin S, Zhang P (2019). ROS production and scavenging in three cherry rootstocks under short-term waterlogging conditions. Scientia Horticulturae 257:108647.

Jogawat A (2019) Osmolytes and their role in abiotic stress tolerance in plants. Molecular Plant Abiotic Stress: Biology and Biotechnology 91-104.

Lal M, Kumari A, Sheokand S (2019). Reactive oxygen species, reactive nitrogen species and oxidative metabolism under waterlogging stress. In: Reactive oxygen, nitrogen and sulfur species in plants pp 777-812.

Leopold AC, Toenniessen RPW (1984). Salinity tolerance in plants. Wiley, New York, pp 407-426.

Liu H (2019). Phenotypic and seedling growth characteristics of different germplasms of green seedling Cinnamomum Camphora (L.) Presl. Revista de la Facultad de Agronomia de la Universidad del Zulia 36:4.

Lu M, Chen M, Song J, Wang Y, Pan Y, Wang C, ... Zhang Y (2019). Anatomy and transcriptome analysis in leaves revealed how nitrogen (N) availability influence drought acclimation of Populus. Trees 33(4):1003-1014.

Majumder B, Das S, Mukhopadhyay S, Biswas AK (2019). Identification of arsenic-tolerant and arsenic-sensitive rice (Oryza sativa L.) cultivars on the basis of arsenic accumulation assisted stress perception, morpho-biochemical responses, and alteration in genomic template stability. Protoplasma 256:193-211.

Naseer S, Lee Y, Lapierre C, Franke R, Nawrath C, Geldner N (2012). Casparian strip diffusion barrier in Arabidopsis is made of a lignin polymer without suberin; Proceedings of the National Academy of Sciences 109(25):10101-10106.

Peng Y, Zhou Z, Zhang Z, Yu X, Zhang X, Du K (2018). Molecular and physiological responses in roots of two full-sib poplars uncover mechanisms that contribute to differences in partial submergence tolerance. Scientific Reports 8(1):12829.

Qi X, Li Q, Ma X, Qian C, Wang H, Ren N, ... Chen X (2019). Waterlogging‐induced adventitious root formation in cucumber is regulated by ethylene and auxin through reactive oxygen species signalling. Plant, Cell & Environment 42(5):1458-1470.

Roberts DJ, Werner DJ (2016). Genome size and ploidy levels of cercis (Redbud) species, cultivars, and botanical varieties. HortScience 51(4):330-333.

Salah A, Zhan M, Cao C, Han Y, Ling L, Liu Z, Jiang Y (2019). γ-Aminobutyric acid promotes chloroplast ultrastructure, antioxidant capacity, and growth of waterlogged maize seedlings. Scientific Reports 9(1):484.

Shiono K, Ejiri M, Shimizu K, Yamada S (2019). Improved waterlogging tolerance of barley (Hordeum vulgare) by pretreatment with ethephon. Plant Production Science 22(2):285-295.

Škute N, Savicka M, Kulbachna A, Petjukevičs A, Harlamova N (2019). Influence of flooding on leaf cell membranes of three Latvian wheat cultivars (Triticum aestivum (L)). In: Proceedings of the 12th International Scientific and Practical Conference. Volume I (Vol. 287, pp 290).

Tian L, Bi W, Liu X, Sun L, Li J (2019). Effects of waterlogging stress on the physiological response and grain-filling characteristics of spring maize (Zea mays L.) under field conditions. Acta Physiologiae Plantarum 41(5):63.

Velasco NF, Ligarreto GA, Díaz HR, Fonseca LPM (2019). Photosynthetic responses and tolerance to root-zone hypoxia stress of five bean cultivars (Phaseolus vulgaris L.). South African Journal of Botany 123:200-207.

Yang C, Zhang X, Li J, Bao M, Ni D, Seago JL (2014). Anatomy and histochemistry of roots and shoots in wild rice (Zizania latifolia Griseb.). Journal of Botany 2014:9. Https://

Yu Q, Shen Y, Wang Q, Wang X, Fan L, Wang Y, ... Zhang M (2019). Light deficiency and waterlogging affect chlorophyll metabolism and photosynthesis in Magnolia sinostellata. Trees 33(1):11-22.

Zeng N, Yang Z, Zhang Z, Hu L, Chen L (2019). Comparative transcriptome combined with proteome analyses revealed key factors involved in alfalfa (Medicago sativa) response to waterlogging stress. International Journal of Molecular Sciences 20(6):1359.

How to Cite
LUO, J., & YUAN, L.-Y. (2020). Responses of physiological activities and lateral root anatomical structure of Cercis glabra to waterlogging stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(3).
Research Articles