Effects of lead on growth, osmotic adjustment, antioxidant activity and photosynthetic responses in Phoebe chekiangensis seedlings

  • Yujie YANG Yangtze University, Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou 434020, Hubei
  • Wenjie LI Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Xinru HE Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Die HU Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Yongjun FEI Yangtze University, Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou 434020, Hubei
Keywords: growth; lead stress; Phoebe chekiangensis; photosynthetic parameters

Abstract

Experiments were conducted on 1-year Phoebe chekiangensis seedlings treated by different concentration (0, 300, 600, 900, 1200 mg/L) of Pb (NO3)2. Sixty days later, determination was implemented on seedling growth, physiological and photosynthetic parameters. The results showed that the lower concentration treated could promote the growth of the seedlings. But with the increase of concentration of lead, P. chekiangensis seedling height increment, ground diameter growth, whole biomass, total root surface area, root volume, total root length and root activity decreased, while root-shoot ratio present a rising trend. With the increase of concentration of Pb(NO3)2 solution, the membrane permeability and MDA content of P. chekiangensis seedlings showed a trend of rise after the first reduce; the protein content and chlorophyll content presented a trend of decrease after the first increase; while the POD, SOD and CAT activity increased firstly but decreased afterwards; the net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate were all increase at first then decrease, which indicated that protection enzyme activity and membrane was damaged thus the growth of P. chekiangensis seedlings was inhibited.

 

***

In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. The article is to be paginated when the complete issue will be ready for publishing (Volume 48, Issue 3, 2020). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

Metrics

Metrics Loading ...

References

Arya SK, Basu A, Mukherjee A (2013). Lead induced genotoxicity and cytotoxicity in root cells of Allium cepa and Vicia faba. Nucleus 56(3):183-189. https://doi.org/10.1007/s13237-013-0099-z

Dalton DA (1995). Antioxidant defenses of plants and fungi. In: Sami A (ed). Oxidative stress and antioxidant defenses in biology. Chapman & Hall, New York, pp 298-354.

Foyer CH, Descourvieres P, Kunert KJ (1994). Protection against oxygen radicals: An important defense mechanism studied in transgenic plants. Plant, Cell and Environment 17(5):507-523.

Ghnaya T, Zaier H, Baioui R, Sghaier S, Lucchini G, Sacchi AG, … Abdelly C (2013). Implication of organic acids in the long-distance transport and the accumulation of lead in Sesuvium portulacastrum and Brassica juncea. Chemosphere 90(4):1449-1454. https://doi.org/10.1016/j.chemosphere.2012.08.061

Kasim WA, Abokassem EM, Ragab GA, Sewelam NA (2014). Alleviation of lead stress toxicity in Vigna unguiculata by salicylic acid. Egyptian Journal of Experimental Biology 10:37-49.

Huang B (2017). The technique of bud propagation in tissue culture of Phoebe chekiangensis. Fujian Linye pp 45-48.

Ing CP, David WM, Leung, H, Harry T, David JB (2011). Correlation of growth inhibition with accumulation of Pb in cell wall and changes in response to oxidative stress in Arabidopsis thaliana seedlings. Plant Growth Regulation 64:17-25.

Lamhamdi M, Galiou OE, Bakrim A, Nóvoa-Muñoz JC, Arias-Estévez M, Aarab A, Lafont R (2013). Effect of lead stress on mineral content and growth of wheat (Triticum aestivum) and spinach (Spinacia oleracea) seedlings. Saudi Journal of Biological Sciences 20(1):29-36. https://doi.org/10.1016/j.sjbs.2012.09.001

Li DL, Xiang QB (2004). Effects of light condition on the growth and photosynthetic characters of Phoebe chekiangensis seedings. Journal of Nanjing Forestry University (Natural Sciences Edition) 28(5):27-31. http://www.cje.net.cn/Jwk3_stxzz/stxzz/EN/

Li DL, Xiang QB (2006). Effects of soil moisture status on the Phoebe chekiangensis seedings. Journal of Nanjing Forestry University (Natural Sciences Edition) 30(5):112-114.

Li YG, Liu XH, Ma JW, Li FF, Shi CG, Yang SZ (2015). Effects of cultivation measures on growth and root morphology of container seedlings of Phoebe chekiangensis. Journal of Northeast Forestry University 43(1):41-44.

Lin JH, Li J, Li G, Dai BX, Shi XL, Shen J, Hu YY, Wu JS, Song LL (2017). Effect of Lead Stress on Growth and Physiological Properties in Torreya grandis Seedlings. Journal of Zhejiang Forestry Science and Technology 37(1):33-40.

Ma LQ, Komar KM, Tu C, Zhang W, Cai Y, Kennelley ED (2001). A fern that hyperaccumulates arsenic. Nature 409(6820):579. https://doi.org/10.1038/35054664

Malecka A, Jarmuszkiewicz W, Tomaszewsk B (2001). Antioxidative defense to lead stress in subcellular compartments of pea root cells. Acta Biochimica Polonica 48(3):687-698.

Michalak A (2006). Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies 15(4):523-530.

Phang C, Leung DW, Taylor HH, Burritt DJ (2011). Correlation of growth inhibition with accumulation of Pb in cell wall and changes in response to oxidative stress in Arabidopsis thaliana seedlings. Plant Growth Regulation 64(1):17-25. https://doi.org/10.1007/s10725-010-9527-0

Qiu YB, Qiao WY, Liu J, Jiang JM, Jiang AP (2016). Influence of container size, substrate and fertilizer on big container-growing seedling quality of Phoebe chekiangensis. Journal of Northeast Forestry University 44(9):20-23.

Raikwar MK, Kumar P, Singh M, Singh A (2008). Toxic effect of heavy metals in livestock health. Veterinary World 1(1):28.

Seregin IV, Kozhevnikova AD (2008). Roles of root and shoot tissues in transport and accumulation of cadmium, lead, nickel, and strontium. Russian Journal of Plant Physiology 55(1):1-22. https://doi.org/10.1134/S1021443708010019

Shameem R, Asif A, Masooma NC (2016). Plants tolerance mechanism and physiological effects under heavy metals stress. International Journal of Agricultural and Environmental Research 2(2):125-132.

Sharma P, Dubey RS (2005). Lead toxicity in plants. Brazilian Journal of Plant Physiology 17(1):35-52. http://dx.doi.org/10.1590/s1677-04202005000100004

Sharmaa, RK, Agrawala M, Marshall F (2007). Heavy metal contamination of soil and vegetables in suburban areas of Varanasi, India. Ecotoxicology and Environmental Safety 66(2):258-266. https://doi.org/10.1016/j.ecoenv.2005.11.007

Singh RP, Tripathi RD, Sinha SK, Maheshwari R, Srivastava HS (1997). Response of higher plants to lead contaminated environment. Chemosphere 34(11):2467-2493. https://doi.org/10.1016/s0045-6535(97)00087-8

Verma S, Dubey RS (2003). Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science 164(4):645-655. https://doi.org/10.1016/S0168-9452(03)00022-0

Wang Y, Wang X, Wu X, Zhang L, Wu L, Xu Y, Zhou Z (2013). Effects of slow-release fertilizer loading on growth and construction of nutrients reserves of Phoebe chekiangensis and Phoebe bournei container seedlings. Scientia Silvae Sinicae 49(12):57-63.

Wang Y, Wang X, Zhang L, Wu L, Zhou Z, Xu Y (2013). Effects of different cultivation substrates on growth and root system development of container seedlings of Phoebe chekiangensis and P. bournei. Journal of Plant Resources and Environment 22(3):81-87.

Wierzbicka M (1995). How lead loses its toxicity to plants. Acta Societatis Botanicorum Poloniae 64(1):81-90. https://doi.org/10.5586/asbp.1995.012

Winska-Krysiak M, Koropacka K, Gawronski S (2015). Determination of the tolerance of sunflower to lead-induced stress. Journal of Elementology 20(2). https://doi.org/10.5601/jelem.2014.19.4.721

Xiang Q (1974). A new species in Phoebe genus-Phoebe chekiangensis. Acta Phytotaxonomica Sincia 12(3):295-297.

Yadav SK (2010). Heavy metals toxicity in plants: An overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. South African Journal of Botany 76:167-179. https://doi.org/10.1016/j.sajb.2009.10.007

Yan ZZ, Tam NF (2011). Temporal changes of polyphenols and enzyme activities in seedlings of Kandelia obovata under lead and manganese stresses. Marine Pollution Bulletin 63(5-12):438–444.

Zang M, Xiaolan Q, Lifang Y (2015). Analysis of structure features and species diversity of Phoebe chekiangensis community in sanqingshan mountain of Jiangxi province. Journal of Anhui Normal University (Natural Science) 38(3):267-271.

Zheng S, Jiang L, Teng We, Pan H, Wen M, Hou W, Hu H, Wang L (2015). Growth and physiological mechanism of Machilus pauhoi seedlings in response to lead nitrate stress. Journal of Forestry Engineering 29(3):25-30.

Zhou J, Jiang Z, Ma J, Yang L, Wei Y (2017). The effects of lead stress on photosynthetic function and chloroplast ultrastructure of Robinia pseudoacacia seedlings. Environmental Science and Pollution Research 24(11):10718-10726. https://doi.org/10.1007/s11356-017-8713-2

Published
2020-08-31
How to Cite
YANG, Y., LI, W., HE, X., HU, D., & FEI, Y. (2020). Effects of lead on growth, osmotic adjustment, antioxidant activity and photosynthetic responses in Phoebe chekiangensis seedlings. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(3). https://doi.org/10.15835/nbha48311933
Section
Research Articles