Molecular responses of arbuscular mycorrhizal fungi in tolerating root rot of trifoliate orange

Shen CHENG; Li TIAN; Ying-Ning ZOU; Qiang-Sheng WU; Kamil KUČA; Popy BORA

doi:10.15835/nbha48211916

Authors

Shen CHENG Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN)
Li TIAN Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN)
Ying-Ning ZOU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN)
Qiang-Sheng WU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN) http://orcid.org/0000-0002-3405-8409
Kamil KUČA University of Hradec Kralove, Faculty of Science, Department of Chemistry, Hradec Kralove 50003 (CZ)
Popy BORA Assam Agricultural University, Department of Plant Pathology, Jorhat (IN)

DOI:

https://doi.org/10.15835/nbha48211916

Keywords:

arbuscular mycorrhizal fungi; citrus; pathogen; RNA-Seq

Abstract

Arbuscular mycorrhizal fungi (AMF) enhance plant disease resistance, while the underlying mechanisms in the molecular levels are not yet known. In this study, five-leaf-old trifoliate orange seedlings were inoculated with Funneliformis mosseae for 14 weeks and subsequently were infected by a citrus root rot pathogen Phytophthora parasitica by 7 days. The transcriptome results by Illumina HiSeq 4000 revealed that the percentage of Q30 bases reached 92.99% or above, and 29696 unigenes were annotated in a total of 63531 unigenes. 654 and 103 differentially expressed genes (DEGs) were respectively annotated in AMF-inoculated versus non-AMF-inoculated plants under non-infection and infection with P. parasitica, respectively, whilst these DEGs were related to defense mechanisms, signal transduction mechanisms and secondary metabolites biosynthesis. Forty-two genes were functionally annotated as the putative 'defense mechanism', whilst AMF inoculation induced 1 gene down-regulated and 3 genes up-regulated under P. parasitica infection. AMF inoculation stimulated more genes linked to signal transduction mechanism down-regulated than non-AMF plants. Eight genes were involved in secondary metabolites biosynthesis in AMF versus non-AMF seedlings under P. parasitica-infection conditions. Such transcriptome database provided total information in the molecular levels regarding mycorrhizal roles in tolerating Phytophthora parasitica infection.

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