MAMP-triggered resistance induced by elicitor protein PeBA1 derived from Bacillus amyloliquefaciens NC6 in common bean (Phaseolus vulgaris L.) against green peach aphid (Myzus persicae Sulzer)

  • Abdul BASIT Guizhou University, Department of Plant Pathology, Agriculture College, Guiyang 550025
  • Tala NAZIR Guizhou University, Department of Plant Pathology, Agriculture College, Guiyang 550025
  • Abdul HANAN Guizhou University, Department of Plant Pathology, Agriculture College, Guiyang 550025
  • Muhammad Z. MAJEED University of Sargodha, Department of Entomology, College of Agriculture, Sargodha 40100
  • Muhammad FARHAN Guizhou University, Department of Plant Pathology, Agriculture College, Guiyang 550025
  • Dewen QIU Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Beijing 100081
  • Yong WANG Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Beijing 100081
Keywords: elicitor protein; jasmonic acid pathway; induced resistance; life-history traits; Myzus persicae; PeBA1; plant defense hormones; salicylic acid pathway

Abstract

Bacterial microbe-associated molecular patterns (MAMPs) play an important role in innate plant immunity. This in vitro study evaluated the putative role of protein elicitor PeBA1 derived from Bacillus amyloliquefaciens NC6 strain in eliciting induced resistance type responses in common bean (Phaseolus vulgaris) plants against green peach aphid Myzus persicae. Nymphal developmental time of aphids was significantly prolonged and the fecundity was significantly reduced by different concentrations of PeBA1 elicitor (i.e. 40.51, 24.91 and 16.38 µg mL-1) applied at three different temperature regimes (i.e. 21, 27 and 30 °C). Moreover, foliar application of PeBA1 elicitor protein strongly up-regulated the expression levels of salicylic acid (SA) pathway-associated genes, while the expression levels of jasmonic acid (JA) pathway-associated genes exhibited a moderate induction. Quantification by LC/MSMS revealed a linear increase of both SA and JA plant defense hormones along with the time of exposure. Our findings suggest that the bacterial elicitor protein PeBA1 could be used as an effective biological pest management tool against phloem-feeding insect pests such as green peach aphids M. persicae.

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Published
2020-06-30
How to Cite
BASIT, A., NAZIR, T., HANAN, A., Z. MAJEED, M., FARHAN, M., QIU, D., & WANG, Y. (2020). MAMP-triggered resistance induced by elicitor protein PeBA1 derived from Bacillus amyloliquefaciens NC6 in common bean (Phaseolus vulgaris L.) against green peach aphid (Myzus persicae Sulzer). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(2), 705-715. https://doi.org/10.15835/nbha48211901
Section
Research Articles